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Citations for Roche :
2301 - 2350 of 2456 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... cDNA expressing vectors along with retroviral packaging vectors Gag-Pol and VSG-G were transfected into HEK293T cells using X-tremeGENE 9 DNA Transfection reagent (Roche #6364787001). Virus-containing supernatant was collected 48 hours after transfected and passed through a 0.45μm filter ...
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bioRxiv - Cell Biology 2022Quote: ... sgRNA expressing vectors along with lentiviral packaging vectors Delta-VPR and VSV-G were transfected into HEK293T cells using X-tremeGENE 9 DNA Transfection reagent (Roche #6364787001). cDNA expressing vectors along with retroviral packaging vectors Gag-Pol and VSG-G were transfected into HEK293T cells using X-tremeGENE 9 DNA Transfection reagent (Roche #6364787001) ...
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bioRxiv - Biochemistry 2022Quote: ... coli cells were lysed by sonication on ice in PBS pH 7.4 with protease inhibitors (cOmplete, EDTA-free, Roche Applied Science). The protein was purified over Glutathione Sepharose 4 Fast Flow (GE Healthcare ...
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bioRxiv - Bioengineering 2022Quote: ... the cells were rinsed with PBS and incubated with 5% (v/v) normal donkey serum and 1% (w/v) BSA (Roche, 10.7350.86001) in PBS for 1 hour at room temperature to block the non-specific antibody binding ...
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bioRxiv - Bioengineering 2022Quote: ... Encapsulated or adherent cells were plated in a 96 well plate for 24 or 48h when WST-1 reagent (Roche, Switzerland) was added ...
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bioRxiv - Cell Biology 2022Quote: ... IFNγ-R2-eGFP plasmid was transfected in HeLa or Caco-2 cells with X-tremeGENE HP DNA transfection reagent (Roche, 6366236001) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Tissues or cells were lysed in NP-40 lysis buffer (Beyotime P0013F) supplemented with protease inhibitor cocktail (complete Mini, Roche 04693124001), phosphatase inhibitor cocktail (PhosSTOP ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells were maintained in growing media (DMEM + Glutamax, 10% FBS, pen/strep 5ml) supplemented with 0.8mg/ml G418 (Roche, G418-RO) for maintenance or removed for exposure to chemicals ...
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bioRxiv - Molecular Biology 2022Quote: ... and pellet cells were then resuspended in 400 μL of lysis buffer (PBS 1x supplemented with Roche protease inhibitor and benzonase). Cells were lysed by sonication (30% Amp ...
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bioRxiv - Molecular Biology 2022Quote: ... The recombinant plasmid pcDNA3.1-Orm2 was transiently transfected into LO2 cells for 48 h with X-treme GENETMHP DNA Transfection Reagent (Roche, Basel, Switzerland).
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bioRxiv - Microbiology 2022Quote: ... cell pellet was resuspended in prechilled buffer A (20 mM Tris/HCl, pH: 8) supplemented with cOmplete™ protease inhibitor (Roche) and 5 mM EDTA ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then resuspended in 10 ml of cold PBS and incubated with cOmplete mini EDTA-free protease inhibitor (Roche, 11836170001) for 30 min at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... the cells were harvested from the matrices by digesting the collagen with liberase DL research grade (24 U; Roche, Basel, Switzerland). The cells were then fixed with 4% PFA ...
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bioRxiv - Bioengineering 2024Quote: ... the cells were harvested from the matrices by digesting the collagen with liberase DL research grade (24 U; Roche, Basel, Switzerland). The cells were then fixed with 4% PFA ...
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bioRxiv - Cell Biology 2024Quote: ... assay was performed following our previous publication(4, 55) and the instruction of In Situ Cell Death Detection Kit TMR red (Roche, 12156792910). Cells were cultured on coverslips for 36 h ...
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bioRxiv - Molecular Biology 2024Quote: ... treated with λPPase or that purified from Calyculin A-treated cells was subjected to proteolytic digestion with 0.01 ng/µl trypsin (Roche, sequencing grade) for 0 ...
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bioRxiv - Neuroscience 2024Quote: ... Tissues were washed three times with TBS-T and were incubated with a TUNEL reaction mixture (In Situ Cell Death Detection Kit, TMR red, Roche 12156792910) for 1 h at 37 °C ...
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bioRxiv - Genomics 2023Quote: hTERT RPE-1 cells at 70% confluence were harvested by trypsinization after 2-hour treatment with colcemid (100 ng/ml, Roche 10295892001) to arrest cells in mitosis ...
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bioRxiv - Immunology 2024Quote: Cells proteins were lysed and isolated from cell pellet by RIPA buffer (Thermo, 89901) with cOmplete mini EDTA free protease inhibitor (Roche, 11836170001) on ice incubation for 30 min and centrifugation at 15,000 rpm for 15 min at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... and a sgRNA preceded by a rrk1 leader and followed by a Hammerhead Ribozyme as described in [17] was digested and prepared for gap repair as follows: the vector pJB166 was purified from DH5α bacterial cells using the Genopure plasmid midi kit (Roche, 03143414001) and diluted to 1 μg/μL ...
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bioRxiv - Cell Biology 2023Quote: The tissues from treated mice and HFs were used for TUNEL apoptotic detection (In Situ Cell Death Detection Kit, TMR Red, Roche 12156792910) After fixation and antigen retrieval procedures ...
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bioRxiv - Immunology 2023Quote: ... Wells were pre-coated with 100 μL per well of capture anti-histone antibody contained in the Cell Death Detection ELISA kit (1:40 in 1x coating buffer; Roche 11544675001) or anti-myeloperoxidase (MPO ...
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bioRxiv - Immunology 2023Quote: ... Brain and spinal cord samples specifically processed for the detection of intracellular MBP within phagocytic cells were predigested with 0.5 mg/mL Collagenase/Dispase® (Roche, #11097113001), 0.02 mg/mL DNase I (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: The cellular metabolic viability was measured by NADH-dependent mitochondrial dehydrogenase activity using Cell Proliferation Kit II (XTT) (Roche, Basel, Switzerland) according to the manufacturer’s protocol by measuring the absorbance at 492nm in BioTek Synergy HTX multimode reader ...
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bioRxiv - Cancer Biology 2023Quote: ... This plasmid was digested with PacI and transfected into HEK293 cells using X-tremeGENETM HP DNA transfection reagent (Roche, Basel, Switzerland) to produce infectious viral particles ...
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bioRxiv - Immunology 2023Quote: ... Apoptosis also was detected using a terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP)-biotin nick end-labeling (TUNEL) assay with Cell Death Detection Kit (Roche, Germany). Nuclei were stained with 4-6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Biophysics 2023Quote: ... Recombinant baculoviruses were generated by transfecting 2.5 μg of a transfer bacmid into Sf9 cells (2.5 mL at a density of 106 cells/mL) using 3 μL of X-tremeGENE™ HP DNA Transfection Reagent (Roche) and 100 μL Transfection Medium (Expression Systems) ...
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Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeatsbioRxiv - Molecular Biology 2023Quote: ... each well of cells from 24-well plates was lysed with 120 μL of RIPA buffer with protease inhibitor (Roche, 11836153001). All protein lysates were denatured with 12% β-mercapto-ethanol in 6x SDS-loading dye ...
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bioRxiv - Molecular Biology 2023Quote: ... 250-500k cells were washed twice (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM spermidine, 1× Roche cOmplete protease inhibitors) and attached to 10 uL ConA-coated magnetic beads (Bangs Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were further incubated for 48 h and subsequently subjected to Gentamycin (G418) selection (1 μg/ml, Roche, G418-RO). After 4 days cells were sorted on a fluorescence-activated cell sorter (FACS ...
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bioRxiv - Microbiology 2023Quote: ... 300 mM NaCl and 2 mM β -mercaptoethanol) per 5 × 108 cells in the presence of EDTA-free anti-protease cocktail (complete from Roche). Lysis was performed with two cycles of freezing (− 180 °C ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were rinsed with PBS and incubated with 5% (v/v) normal donkey serum and 1% (w/v) BSA (Roche, 10.7350.86001) in PBS for 1 hour at room temperature to block the non-specific antibody binding ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were resuspended in lysis buffer (8 M urea, 50 mM EPPS pH 8.5, 150 mM NaCl, Roche protease inhibitor tablet) and lysed by bead beating ...
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bioRxiv - Cell Biology 2023Quote: Primary human macrophages were washed once with ice cold PBS prior to harvest of cells with TBS supplemented with protease inhibitors (Complete®, Roche) and phosphatase inhibitors (PhosStop® ...
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Quantitative Comparison of Presenilin Protein Expression Reveals Greater Activity of PS2-γ-SecretasebioRxiv - Cell Biology 2023Quote: ... Cells were scrapped into 100 µl of RIPA lysis buffer (Astral Scientific 786-490) supplemented with protease inhibitor cocktail (Roche 11697498001) and transferred to microfuge tubes ...
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bioRxiv - Developmental Biology 2023Quote: TUNEL staining was performed according to the manufacturer’s protocol with minor modifications (In Situ Cell Death Detection Kit, TMR red; catalogue number 12156792910; Roche, Mannheim, Germany). Briefly ...
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bioRxiv - Pathology 2023Quote: Cells or tissues were homogenized in RIPA buffer supplemented with protease inhibitors and phosphatase inhibitor cocktails (Roche Diagnostics, Indianapolis, IN, USA). Western blot analysis was performed according to the routine procedure ...
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bioRxiv - Neuroscience 2023Quote: ... 300,000 cells were washed twice (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM spermidine, 1× Roche cOmplete protease inhibitors) and attached to 10 ConA-coated magnetic beads (Bangs Laboratories ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed 2X with cold DPBS++ and RIPA buffer (Pierce #89900) containing complete EDTA-free protease inhibitor cocktail (Roche #11873580001) was added to wells ...
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bioRxiv - Cell Biology 2023Quote: ... Infected red blood cells were lysed 10 min on ice with 0.1% saponin in PBS complemented with 1x Protease Inhibitors Cocktail (Roche; PBS+PI). Parasites were pelleted and washed with PBS+PI to remove haemoglobin ...
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bioRxiv - Microbiology 2023Quote: ... The cell pellet was resuspended in chilled lysis buffer (Bugbuster) containing one dissolved cOmplete™ EDTA-free Protease Inhibitor cocktail tablet (Roche). The cell lysates were clarified by centrifugation (11,000 × g ...
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bioRxiv - Genomics 2023Quote: ... sgRNA- or cDNA-expressing vectors and lentiviral packaging vectors Delta-VPR and CMV VSV-G were transfected into HEK293T cells using XTremeGene 9 transfection reagent (Roche #636478700). The supernatant containing virus was collected 48 h after transfection and passed through a 0.45-µm filter ...
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bioRxiv - Cancer Biology 2023Quote: Cells were washed with phosphate-buffered saline (PBS) and lysed in whole cell lysis (WCL) buffer supplemented with cOmplete protease inhibitor cocktail (Roche Diagnostics), 100 μM sodium orthovanadate (NaO ...
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bioRxiv - Cancer Biology 2023Quote: ... unconcentrated cell culture supernatants were incubated with a rabbit monoclonal anti-glycodelin antibody (clone 12C9, kindly provided by ROCHE, Penzberg, Germany) for 1 h at room temperature prior to incubation with immune cells ...
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bioRxiv - Cancer Biology 2024Quote: Cellular proteins were obtained by extracting whole-cell lysates using RIPA lysis buffer enhanced by addition of phosphatase and protease inhibitors (Roche 11836153001). To remove debris ...
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bioRxiv - Cell Biology 2024Quote: The cells were lysed in cold Lysis buffer supplemented with cOmplete mini EDTA-free protease inhibitor cocktail (Cat. No. 11836170001, Roche, Switzerland) and phosphatases inhibitor PhosSTOP™ (Cat ...
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bioRxiv - Molecular Biology 2024Quote: Whole cell extracts from cultured cells were prepared by direct lysis and sonication of cells in 2% SDS sample buffer containing phosphatase and protease inhibitors (Roche, Germany). Cell extracts were separated in denaturing 8% or 15% (used only for CDKN1A ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed and lysed in radioimmunoprecipitation assay buffer (RIPA) buffer supplemented with Complete® protease inhibitor cocktail (Roche, Basel, Switzerland) and Phosphatase Inhibitor Cocktail II (Abcam ...
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bioRxiv - Immunology 2024Quote: ... were resuspended in mHBSS at a density of 107 cells/ml and pretreated with 2 mM Pefabloc SC Plus (Roche #11873601001) for 15 min at 37 °C with rotation set to 10 RPM in a BSA-coated centrifugation tube ...
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bioRxiv - Cell Biology 2024Quote: ... HL-60s were differentiated into a neutrophil-like state by taking an aliquot of cells in their culturing medium and supplementing with an equal volume of Nutridoma-CS (Roche #11363743001) and DMSO diluted in RPMI such that that the final concentrations were 0.2 million/mL HL-60 cells ...