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Citations for Roche :
1801 - 1850 of 2327 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: TUNEL assay [47] was performed using an in situ cell death detection kit conjugated with fluorescein isothiocyanate (Roche, 11684795910). DAPI (Vectashield Antifade Mounting Medium with DAPI ...
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bioRxiv - Cell Biology 2021Quote: ... except that 5 mg of synchronized HeLa cell lysate in 8 M Urea with protease inhibitor cocktail (Roche, 4693116001) was diluted by PBS buffer to a final urea concentration of 1 M before incubating with the conjugated beads ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were harvested and ruptured with a Dounce homogenizer in the buffer A containing cOmplete protein inhibitor cocktail (Roche) and 1mM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Neuroscience 2021Quote: ... PFA was removed and cells were washed 3x with PBS then blocked in a buffer containing 0.2% Triton X-100 (Roche) in 50% normal goat serum (NGS ...
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bioRxiv - Neuroscience 2021Quote: ... Whole cell tissue lysis: Lysis was performed in RIPA buffer supplemented with protease inhibitors (Complete Mini, Roche Applied Science), 0.1 mM PMSF ...
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bioRxiv - Cancer Biology 2021Quote: Cultured cells were scraped and collected in radioimmunoprecipitation assay buffer RIPA buffer including PhosStop and protease inhibitor cocktail (Roche). Xenograft and HCC mouse samples were homogenized in RIPA buffer using metal beads and the LT tissuelyzer (Qiagen) ...
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bioRxiv - Genetics 2021Quote: ... Cell pellets were suspended in equilibration buffer (20 mM Tris pH 8.0, 500 mM NaCl) plus protease inhibitors (Roche) and lysed using a high pressure Emulsiflex ...
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bioRxiv - Immunology 2020Quote: ... These were identified by immunohistochemical staining (IHC) with antibodies against T cells: CD3 (Roche Cat # 790-4341, pre-diluted), CD4 (Roche ...
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bioRxiv - Molecular Biology 2021Quote: (Thawed) Cell pellets were lysed on ice for at least 10 min with 1x protease inhibitors cocktail (PIC; Roche) and 1x quenchers (10 mM NaN3 ...
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bioRxiv - Genomics 2021Quote: ... followed by lysing the cells on ice for 3 min in 50 μl of ATAC-seq RSB containing 0.1% NP40 (Roche), 0.1% Tween-20 (Roche) ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell lysates were extracted with phosphate-buffered saline (PBS) containing 2 % Triton X-100 and protease inhibitors (Roche Complete ...
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bioRxiv - Microbiology 2022Quote: ... 2013)) were transfected in HeLa cells 24 h before infection using X-tremeGENETM HP DNA Transfection Reagent (Roche, 6366244001), according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: Cells were lysed with SLB (10 mM Tris, 150 mM NaCl, 0.5% NP-40) with protease inhibitors (Roche #11873580001) and phosphatase inhibitors (Roche #04906837001 ...
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bioRxiv - Immunology 2022Quote: ... T cells were isolated by dissociating tumor tissue in the presence of collagenase D (1 mg/ml) (Roche-Merck) for 10 min at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... The TUNEL method was used to quantify apoptosis following the manufacturer’s instructions (In Situ Cell Death Detection kit; Roche). The percentage of TUNEL-positive cells in each kidney was quantified as described above ...
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bioRxiv - Cell Biology 2022Quote: ... cells were obtained by lysing cells in PBS containing 0.1% Triton X-100 (PBS/TX) as well as protease and protein inhibitor cocktails (Roche) for 5 min on ice ...
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bioRxiv - Physiology 2022Quote: ... In each reaction 50 ng luciferase reporter and 150 ng expression vectors were transfected into C2C12 myoblasts in 24-well plates (50,000 cells per well) using X-tremeGENE 9 (Roche). 5 ng modified pRL null plasmid (25 ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were harvested and re-suspended in buffer A supplemented with 10 μM ZnCl2 and protease inhibitor (Complete, Roche). Cells were then homogenized by sonication and the crude extract was centrifuged for 30 min at 18,000□×□g ...
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Stromal STAT5-mediated trophic activity regulates hematopoietic multipotent progenitor niche factorsbioRxiv - Cell Biology 2022Quote: Cells were lysed using RIPA lysis and extraction buffer with protease inhibitors and phosphatase inhibitors from Roche (Indianapolis, IN). Protein concentration were determined with the Bio-Rad protein assay (Hercules ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were washed twice with ice cold PBS and scraped into cold PBS with phosphatase inhibitors (PhosSTOP, Roche). Pelleted cells were snap-frozen and lysed with 8M urea buffer with 100nM TEAB (Sigma) ...
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bioRxiv - Developmental Biology 2022Quote: ... TUNEL staining was performed following citrate buffer antigen retrieval using an in situ cell death detection kit (Roche, 11684795910). Positive control slide was treated with deoxyribonuclease to induce DNA damage (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were lysed by passive lysis buffer (25mM Tris-HCl, 150mM NaCl, 0.5% CA630) containing protease inhibitor cocktail (Roche). The cells were lysed in SDS loading buffer and boiled for 5-10min ...
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bioRxiv - Genomics 2022Quote: ... Trim24 knockout TC-1 ES cells (background 129S6/SvEvTac) carrying an RMCE selection cassette (described previously82) were selected under hygromycin (250μg/ml, Roche) for 10 days ...
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bioRxiv - Synthetic Biology 2022Quote: ... ∼600 µL of 0.5 mm diameter glass beads were added and cells were lysed by bead beating on a MagNA Lyser (Roche) three times for 45 s each at 4500 RPM with 2 min rests at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Cells were lysed with lysis buffer (final concentration: 50 μg/ml lysostaphin, 0.8% NP-40, 1x protease inhibitor (Roche), 250 U/ml benzonase and 1 mM MgCl2 in PBS ...
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bioRxiv - Physiology 2022Quote: ... fixed and proceeded with colorimetric immunoassay for the quantification of cell proliferation according to manufacturer instructions (Roche, Germany, 11647229001).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were subsequently washed with phosphate-buffered saline (PBS) and re-suspended in PBS containing protease inhibitors (Roche, Canada). Cells were heated at 54°C for 3 min in a thermal cycler (VeritiPro ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue was incubated with the probe overnight and Arc positive cells were detected with anti-digoxigenin-HRP conjugate (Roche Applied Science Ref # ...
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bioRxiv - Immunology 2022Quote: ... was added and cells were centrifuged at 350g for 7 min at 4°C and resuspended in PBS with 0.5% BSA (Roche) and 1% FBS (Sorting Buffer) ...
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bioRxiv - Immunology 2022Quote: ... Single cell suspensions of two lung samples from mice of the same sex were pooled and washed with a 2X volume of ACK Lysing Buffer (Quality Biological) as above to lyse red blood cells and then were resuspended in PBS with 0.5% BSA (Roche) and 2 mM EDTA (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... cell lysates were prepared in radio-immunoprecipitation assay (RIPA) buffer supplemented with PhosSTOP and complete protease inhibitor cocktail (Roche). Protein concentration was determined by Bradford assay using the Protein Assay Dye Reagent Concentrate (Bio-Rad ...
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Autocrine Sfrp1 inhibits lung fibroblast invasion during transition to injury induced myofibroblastsbioRxiv - Cell Biology 2022Quote: Cultured cells were first washed twice with sterile 1X PBS and subsequently scraped from the wells using a cell scratcher in 200µl lysis buffer (RIPA buffer supplemented with 1x Roche complete mini protease inhibitor cocktail and Phospho-Stop phosphatase inhibitor) ...
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bioRxiv - Molecular Biology 2022Quote: ... flow cytometry analysis was performed on cell suspensions from kidney homogenates that were digested with 0.2 mg/ml liberase (Roche) and 0.1 mg/ml DNase I (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... MCF7 cells were transfected with FAM-labelled hairpins using X-tremeGENE 9 DNA Transfection Reagent (Roche, USA; 1.0 µL). After 16 hours ...
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bioRxiv - Bioengineering 2023Quote: ... cells were released by incubating gels in a cell culture incubator in a protease solution of 2.5 mg/mL collagenase D (Roche) in PBS until gels were fully degraded ...
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bioRxiv - Neuroscience 2022Quote: Cells were collected by first washing them with ice-cold 1x PBS supplemented with 0.5x protease inhibitor cocktail (Roche) and then collecting them with a cell scraper in PBS/0.5x protease inhibitor in an eppendorf tube ...
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bioRxiv - Biochemistry 2023Quote: The cells were resuspended with lysis buffer (20mM Hepes pH7.4, 10mM MgCl2, 100mM KOAc) containing a protease-inhibiting reagent (Roche) (1mL per mg of cells) ...
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bioRxiv - Developmental Biology 2023Quote: ... Livers were then dissected out of the larvae and subjected to TUNEL assay according to manufacturer’s instructions (In Situ Cell Death Detection kit, Fluorescein; Roche). Nuclei were counterstained with Hoechst (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed twice with ice cold PBS then scraped in 500 μL of PBS + protease inhibitors (PIs) (Roche). Cells were centrifuged at 8000 rpm (4◦C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cells were lysed using 200 μL/well cell lysis buffer (100 mM Tris-HCl pH 8.8, 1 % SDS) and protease inhibitors (cOmplete, Roche) on ice for one h ...
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bioRxiv - Biochemistry 2023Quote: ... the cells were lysed in 300 µL hypotonic lysis buffer supplemented with protease inhibitor tablet (Roche, see Vesicle isolation). The lysates were split into two and half was treated with cGAMP ...
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bioRxiv - Developmental Biology 2023Quote: ... B-16 cells were collected after 48 hours of transfection and RNA was extracted using (Roche, RNA isolation Kit). Total RNA was quantified on a nanodrop (Thermofisher ...
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bioRxiv - Immunology 2023Quote: ... These cells were cultured with healthy donor T cells (E:T ratio of 5:1) and different concentrations of Mosunetuzumab (Genentech, Roche): 0 ...
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bioRxiv - Immunology 2023Quote: ... Cells were lysed by sonication in 700 mM NaCl;20 mM HEPES 7.5 containing 1 protease inhibitor tablet (Roche) + 5ul Turbonuclease ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid minigenes were transfected 24 h after cell seeding using X-tremeGENE 9 DNA Transfection Reagent (Roche; Mannheim, Germany). HepG2 ...
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bioRxiv - Immunology 2023Quote: ... the cells were re-stimulated with 5 μg/mL CTH522 or 1 μg/mL phytohemagglutinin (PHA; Roche, cat.: 11249738001) as positive control ...
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bioRxiv - Genomics 2023Quote: ... Cells were washed twice in cold PBS and scraped off the plates in cold PBS containing protease inhibitors (Roche, Complete 11836170001 and Halt ...
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bioRxiv - Immunology 2023Quote: 50,000 sorted cells were washed with PBS and resuspended in Buffer 1 (1X eBioscience Perm/Wash Buffer, 1X Roche cOmplete EDTA-free Protease Inhibitor ...
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bioRxiv - Biochemistry 2023Quote: ... 3 μg pAcBAC3 plasmid was used to transfect RAW 264.7 cells by mixing the DNA with 6 μg X-tremeGENE HP DNA Transfection Reagent (Roche) according to the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2023Quote: ... Minced lung was enzymatically dissociated to a single cell suspension using 1 mg/mL collagenase A (Roche, Cat# 10103578001), 2-4U/mL elastase (Worthington ...