Labshake search
Citations for Roche :
2101 - 2150 of 2327 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... and from WT and Hcar1 KO mice were lysed in RIPA buffer (cell signaling) supplemented with 0.1 mg/ml PMSF and proteinase inhibitor cocktail (11697498001, Roche, USA), and then were homogenized thoroughly (BRANSON Sonifier 150 ...
-
bioRxiv - Cell Biology 2023Quote: Frozen liver tissues that were crushed using a Multi-beads Shocker (Yasui Kikai, Japan) or cultured cells were dissolved in RIPA buffer containing 1× cOmplete proteinase Inhibitor Cocktail (Roche). Protein concentration in the lysates was determined using a BCA Protein Assay Kit (Thermo Fisher Scientific) ...
-
bioRxiv - Biophysics 2024Quote: ... Tumor spheroids were digested into single cell suspensions using 0.028 Wünsch units/ml of Liberase TM Research Grade (Roche, Mannheim, Germany) and 20 μg/ml of DNase I in L-15 with 10 % CS for 1 hour at 37 °C and 250 RPM on an orbital shaker ...
-
bioRxiv - Systems Biology 2024Quote: ... The treated lymphoma cells were cooled down by addition of the three-fold volume of ice-cold DPBS supplemented with 1xPhosSTOP (Roche) and 100µM sodium orthovanadate ...
-
bioRxiv - Neuroscience 2024Quote: ... proteins were extracted by resuspending cells from 6-well plates in 100 μl of RIPA or 7M Urea Buffer containing protease inhibitor (11873580001, Roche) and ...
-
bioRxiv - Biochemistry 2024Quote: ... Cells were then washed with PBS for three times and lysed with RIPA buffer supplemented with protease inhibitor cocktail (Roche) on ice for 10 min ...
-
bioRxiv - Molecular Biology 2024Quote: ... cells were lysed 80 h post seeding in RIPA buffer (Thermo, 89901) containing cOmplete EDTA-free protease inhibitor cocktail (Roche). Lysate were centrifuged at 15000 rpm for 15 min ...
-
bioRxiv - Pathology 2024Quote: Protein lysate samples were prepared from snap frozen aortas and cells in RIPA solution (Cat# P0013B; Beyotime) supplemented with protease inhibitor (Cat# 05892791001; Roche). Denatured protein lysates were resolved by 8% −10% (wt/vol ...
-
bioRxiv - Cancer Biology 2024Quote: Cell lysates were prepared using a 1% triton X-100 containing lysis buffer (CST) supplemented with protease inhibitor cocktail (Roche) on ice for 15 min ...
-
bioRxiv - Molecular Biology 2023Quote: ... and 100 ng of pCAG-mCherry were transfected into 80% confluent HEK293T cells in 24-well plates using FuGene6 (Roche) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2023Quote: Cell viability upon transduction of tBID death reporter was assessed in 96-well plates through WST-1 assay (Roche, Switzerland) according to the manufacturers’ instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... a total of 0.4–0.5 μg of plasmid and dsRNAs were transfected into BmN4 cells (4–6 × 104 cells per glass bottom 35 mm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche) and cells were fixed 5–6 days later ...
-
bioRxiv - Microbiology 2024Quote: ... spleens were collected from naïve C57BL/6 (CD45.1+) and a single cell suspension was obtained by incubation with Liberase Blendzyme 3 (Roche, Bradford, CT) and DNase (Sigma ...
-
bioRxiv - Neuroscience 2024Quote: hiPSC-DANs cell pellets were collected in ice-cold PBS and were homogenised in RIPA buffer containing protease (cOmplete EDTA-free, Roche) and phosphatase inhibitors (PhosSTOP ...
-
bioRxiv - Cancer Biology 2024Quote: ... PDOs were pooled from 6 wells and incubated for 1.5 hours on ice in Cell Recovery Buffer supplemented with phosphatase inhibitors (Roche, 4906845001). PDOs were lysed using RIPA buffer (Boston BioProducts ...
-
bioRxiv - Cancer Biology 2024Quote: ... cells were washed with PBS and lysed in radioimmunoprecipitation assay (RIPA) buffer supplemented with protease (SERVA) and phosphatase inhibitors (Roche). The protein concentration was determined using a Bicinchoninic Acid Protein (BCA ...
-
bioRxiv - Molecular Biology 2023Quote: ... Transfection of 106 cells in a 6-well plate was performed using 5 μl of transfection agent (X-tremeGENE HP Reagent, Roche), 10 μL of bacmid and 85 μL of SF900II-SFM medium (Gibco) ...
-
bioRxiv - Bioengineering 2024Quote: ... were purchased from Lonza and cultured in endothelial media (VascuLife, Lifeline cell systems) on T75 or T150 flasks coated with 50 μg/ml rat tail collagen I (Roche). HUVEC were transduced to stably express cytoplasmic RFP (LentiBrite RFP Control Lentiviral Biosensor ...
-
bioRxiv - Bioengineering 2024Quote: cDNA from cells was diluted 1:10 and qPCR was performed in triplicate using LightCycler 480 SYBR Green I Mastermix (Roche). Samples containing primers specific for lmna ...
-
bioRxiv - Cancer Biology 2024Quote: The Terminal dUTP Nick End Labeling (TUNEL) and In Situ Cell Death Detection Kits (TMR Red #12156792910; Roche Applied Science) were used to examine apoptosis ...
-
bioRxiv - Immunology 2024Quote: The retroviral recombination substrate pINV-12/23 was introduced into primary leukemic cells utilizing X-treme GENE HP DNA Transfection Reagent (Roche). Recombina-tion efficiency of pINV-12/23 was evaluated through flow cytometry analysis for mouse CD90 (mCD90 ...
-
bioRxiv - Biochemistry 2024Quote: ... The pcDNA3 constructs were used for transient transfection in HEK293 cells using an X-tremeGENE HP DNA Transfection Reagent (Roche, 6366236001according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... Lentiviral vectors expressing sgRNAs were transfected into HEK293T cells with lentiviral packaging vectors CMV VSV-G and psPAX2 using XtremeGene 9 transfection reagent (Roche). After 24 h ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were washed with cold PBS and lysed in IP lysis buffer supplemented with protease inhibitors (Roche, Basel, Switzerland, #05892791001). The supernatant was obtained by centrifugation at 10000 × g for 10 min at 4 °C ...
-
bioRxiv - Synthetic Biology 2024Quote: ... transfected HEK293T cells were harvested at the indicated time points and resuspended in ice-cold RIPA buffer with protease inhibitor cocktail (Roche). Total protein was quantified by BCA assay (Pierce) ...
-
bioRxiv - Cell Biology 2024Quote: ... Protein purification started by quickly thawing the cells in ∼15 mL room-temperature 10mM imidazole with protease inhibitor cocktail (Roche) and freshly added 200µM PMSF ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... The wells containing fixed cells were washed once with 1X PBS and stained with DAPI (50 μg/ml) for (Roche, 10236276001) 10 min at RT and protected from light ...
-
bioRxiv - Immunology 2020Quote: ... The proliferation rate of lymphocytes was measured using 5-Bromo-2-deoxyuridine (BrDU) assay as per the manufacturer instructions (Cell Proliferation ELISA BrDU, Roche, USA).
-
bioRxiv - Molecular Biology 2020Quote: ... washed with ice-cold PBS and re-suspended with 500µL of ‘IP-MS Cell Lysis Buffer’ which was supplemented with 1x cOmplete™ Protease Inhibitor Cocktail (Roche, 11697498001) and 1x PhosSTOP™ (Roche ...
-
bioRxiv - Immunology 2019Quote: ... 300 ng of total RNA from three biological replicates per B cell subset was used as input for the KAPA stranded mRNA-seq Kit with mRNA capture beads (KAPA Biosystems). Libraries were assessed for quality on a bioanalyzer ...
-
bioRxiv - Microbiology 2019Quote: ... Twenty-four hours post-transfection cells were rinsed with PBS and lysed in NP40 buffer supplemented with cOmplete™ protease inhibitor cocktail (Roche). Lysates were clarified by centrifugation and incubated with anti-FLAG M2 magnetic beads (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2019Quote: Cells were lysed in M-PER mammalian protein extraction reagent (Thermo, MA, USA) with protease and phosphatase inhibitors (Roche, Basel, Switzerland), and proteins were quantified using protein assays (Bio-Rad Laboratories ...
-
bioRxiv - Molecular Biology 2019Quote: ... DNA was added to a 50 µL aliquot of cells in addition to 50 µg boiled fish sperm carrier DNA (Roche 11467140001). Cells were equilibrated at 30° for 10 minutes after which 0.5 mL 40% Polyethylene glycol (PEG4000 ...
-
bioRxiv - Genomics 2019Quote: ... Quality control for primer target amplification and performance in multiplex PCR was assessed by screening each primer pair in singleplex reactions using gDNA from PC3 or DU145 cells (10 ng) as template (see below) using the HiFi HotStart Uracil+ ReadyMix PCR Kit (Kapa Biosystems). Reactions were supplemented with uracil N-glycosylase (0.2 U ...
-
bioRxiv - Genetics 2019Quote: ... was used as a hypotonic solution and cells were dropped onto a slide with 1% PFA containing protease inhibitors (Roche 1836153). After 3h at 4°C slides were washed and blocked with 0.5x blocking buffer (1.5% BSA ...
-
bioRxiv - Cancer Biology 2020Quote: ... The cells were washed twice in cold PBS and total RNA was extracted using a High Pure RNA isolation kit (Roche 11828665001). Reverse transcribed RNA was used for sequencing by the University of Lausanne ...
-
bioRxiv - Neuroscience 2020Quote: TUNEL staining was performed according to the manufacturer’s instructions (In Situ Cell Death Detection Kit, Fluorescein, Roche, #11 684 795 910). In brief ...
-
bioRxiv - Neuroscience 2021Quote: Cytotoxicity in the cell cultures (primary cortical neurons and cell lines) was assessed using the cytotoxicity lactate dehydrogenase (LDH) detection kit according to the manufacturer’s instructions (Roche, Basel, Switzerland). Furthermore ...
-
bioRxiv - Biochemistry 2022Quote: Transfection of plasmid DNA was conducted in 293T cells using Lipofectamine 3000 (Life Technology) and X-tremeGENE™ HP DNA Transfection Reagent (Roche) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: Sequencing libraries were prepared using 100-500 ng of cell free DNA (cfDNA) or sonicated genomic DNA using KAPA Hyper-Prep Kit (KAPA Biosystems) with Agilent SureSelect XT Target Enrichment System and Human All Exon V5 capture baits (Agilent Technologies) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were harvested from frozen plates by scraping into RIPA buffer containing cOmplete Mini EDTA-free Protease Inhibitor Cocktail (Roche 11836170001) and PhosSTOP Phosphatase Inhibitor Cocktail Tablets (Roche 04906845001 ...
-
bioRxiv - Cancer Biology 2020Quote: Cell (1 × 104) proliferation was measured using the 5-Bromo-2′-deoxy-uridine Labeling and Detection Kit III (Roche, Mannheim, Germany) (Jin ...
-
bioRxiv - Cancer Biology 2020Quote: Real-time measurements of cell migration and invasion were performed using the CIM-Plate 16 module of an xCELLigence System RTCA DP real-time cell analyzer (Roche, UK). For invasion measurement ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Animals were then re-suspended on ice in 50 µl of RIPA buffer with added phosphatase inhibitors (Cell Signalling Technologies #9806) and complete EDTA-free protease inhibitor cocktail (Roche # 04693159001) before sonication ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... assay [31] was performed on cryosections of retinal explants using an in situ cell death detection kit conjugated with fluorescein isothiocyanate (Roche, 11684795910). DAPI (Vectashield Antifade Mounting Medium with DAPI ...
-
bioRxiv - Molecular Biology 2020Quote: ... were added and after an additional 6 hours BrdU was added and a cell proliferation assay was performed according to the manufacturer’s instructions (Roche Applied Sciences).
-
bioRxiv - Cancer Biology 2020Quote: Neurosphere cell cultures and PC3 were synchronized at metaphase by treating with 80 ng/ml Colcemid (Roche, 10-295-892-001) overnight ...
-
bioRxiv - Neuroscience 2020Quote: ... The stained cells were further labelled by TUNEL (TdT-mediated dUTP-X nick end labeling) with an In-Situ Cell Detection Kit (TMR red) from Roche (12156792910). Fluorescent images were collected on a Zeiss Automatic stage microscope with Zen blue software ...
-
bioRxiv - Cell Biology 2022Quote: Murine cells or aortic tissue were lysed in RIPA buffer containing complete protease inhibitor and phosphoSTOP phosphatase cocktails (Roche Applied Science). Lysate was centrifuged at 10,000 g for 10 min at 4°C ...
-
bioRxiv - Biochemistry 2022Quote: ... The plasmid was transfected into Neuro2a cells and clonal lines selected by incubation with Hygromycin (300 mg/ml, Roche (Nutley, NJ)) ...