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Citations for Roche :
1851 - 1900 of 2327 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... isolated cell wall proteins were probed with anti-HA peroxidase-conjugated antibody (1:1,250 dilution; 11667475001, Roche, Basel, Switzerland).
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bioRxiv - Biochemistry 2023Quote: ... the cells were lysed in 300 µL hypotonic lysis buffer supplemented with protease inhibitor tablet (Roche, see Vesicle isolation). The lysates were split into two and half was treated with cGAMP ...
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bioRxiv - Developmental Biology 2023Quote: ... Livers were then dissected out of the larvae and subjected to TUNEL assay according to manufacturer’s instructions (In Situ Cell Death Detection kit, Fluorescein; Roche). Nuclei were counterstained with Hoechst (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: The cells were resuspended with lysis buffer (20mM Hepes pH7.4, 10mM MgCl2, 100mM KOAc) containing a protease-inhibiting reagent (Roche) (1mL per mg of cells) ...
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bioRxiv - Immunology 2023Quote: ... The final sodium concentrations in the cell culture media were confirmed by potentiometry with a Cobas 8000 analyzer (Roche). T cells were stimulated with plate-bound CD3 (1 μg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... These CPER products were then transfected into Huh7.5 cells using X-tremeGENE™ HP DNA (Roche 6366236001, Basel, Switzerland).
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Emergence of RNA-guided transcription factors via domestication of transposon-encoded TnpB nucleasesbioRxiv - Genetics 2023Quote: Flash-frozen cell pellets were resuspended in 1.2 ml RIP lysis buffer supplemented with cOmplete Protease Inhibitor Cocktail (Roche) and SUPERase•In RNase Inhibitor (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... TUNEL staining was performed as in (46) using the TMR red in situ cell death detection kit (Roche, 12156792910).
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bioRxiv - Cancer Biology 2023Quote: ... FFPE slides were steamed for 48 minutes in Cell Conditioning 1 (CC1) solution (Roche Diagnostics, Cat. No. 950-500) for antigen retrieval ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue slides were steamed for 32 minutes in Cell Conditioning 1 (CC1) solution (Roche Diagnostics, Cat. No. 950-500) for antigen retrieval ...
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bioRxiv - Cancer Biology 2023Quote: RNA extraction from A549 cells grown in 6-well plates was done using High Pure RNA isolation kit (Roche). Transcriptor First strand kit (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... Approximately 20 x 106 cells were used for isolating genomic DNA with High Pure PCR Template Preparation Kit (Roche) as described by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: MTT activity was determined using the Cell Proliferation Kit I according to the recommendations of the manufacturer (Roche, Spain). Optical density was determined at 575[nm with a reference wavelength of 690[nm using a Varioskan Flash spectrophotometer (Thermo Scientific ...
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bioRxiv - Immunology 2024Quote: ... mRNA was isolated from cells and reverse transcribed into cDNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche). Gene expression was quantified using FastStart Roche SybrGreen (Roche) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were resuspended in a 1:1 ratio (w/v) in PB supplemented with Complete Protease Inhibitor Cocktail (Roche) and afterward dropwise frozen in liquid nitrogen before lysed in 6875D LARGE FREEZER/MILL (SPEX SamplePrep LLC) ...
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bioRxiv - Physiology 2024Quote: ... RNA oligonucleotides were delivered to MLO-Y4 cells using the X-treme Gene siRNA Transfection Reagent (Roche; Cat#04476093001) according to a protocol provided by the manufacturer ...
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bioRxiv - Neuroscience 2024Quote: ... 30 μm sections were stained according to instruction of In situ Cell Death Detection Kit (Roche, Basel, Switzerland, #11684817910). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... at 10 mL/g of cell pellet) including protease inhibitors (complete protease inhibitor cocktail tablet, Roche Life Sciences, UK). The suspension was lysed using an Emulsiflex extruding homogenizer (Avestin ...
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bioRxiv - Cell Biology 2024Quote: ... and the cell pellet was gently resuspended in 1 ml ice-cold vPBS containing EDTA-free protease inhibitor (Roche). The cells were again pelleted by centrifugation (1000 x g ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were then washed with PBS 3 times and lysed with RIPA buffer supplemented with protease inhibitor cocktail (Roche), 0.1% Benzonase (Millipore-Sigma) ...
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bioRxiv - Molecular Biology 2023Quote: ... MIO-M1 cells were transfected with 20 µg/ml of pEGFP or pEGFP-SIK2 vector using FugeneHD (Roche, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK cells were lysed in 1xPBS pH 7.4 containing Protease Inhibitor Cocktail tablet (1 tablet/10 mL) (Roche Diagnostics) and separated in 4–16% gradient Native pre-cast gel (SERVA Electrophoresis GmbH) ...
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bioRxiv - Molecular Biology 2024Quote: Cells were collected 48 hours after transfection in 1xPBS pH 7.4 with Protease and Phosphatase Inhibitor Cocktail (Roche Diagnostics). The lysates were sonicated for 10 seconds at 40–50% power ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cell viability was determined by using the WST-1 assay according to the manufacturer’s instructions (Roche Diagnostics, Mannheim, Germany). After removing the cell supernatants for cytotoxicity measurements ...
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bioRxiv - Neuroscience 2024Quote: ... 5-diphenyl tetrazolium bromide (MTT) in accordance with the manufacturer’s recommendations (Cell Proliferation Kit I; Roche Diagnostics, Basel, Switzerland). In brief ...
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bioRxiv - Microbiology 2024Quote: ... The cell pellets were lysed in 8M urea/50mM Tris pH 8.1/100mM NaCl+ protease inhibitors (Roche cat # 4693116001) and quantified by BCA assay (Pierce cat # 23223) ...
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bioRxiv - Genomics 2024Quote: ... whole blood (14.0 – 17.0 mL per subject) was collected using Cell-Free DNA Collection tubes (Roche Diagnostics; Rotkreuz, Switzerland), with no in-clinic processing (e.g. ...
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bioRxiv - Cancer Biology 2024Quote: Total proteins were extracted from cultured NB cells using RIPA buffer supplemented with protease and phosphatase inhibitor cocktail (Roche). The cell lysates were pre-cleared with protein A or protein G beads (Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell volume was determined again and 2 volumes cold buffer A++ (Buffer A + cOmplete protease inhibitors (Roche, cat 04693159001) and 0.15% NP40) ...
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bioRxiv - Cancer Biology 2024Quote: Cells were harvested and lysed with 1x RIPA buffer (CST, #9806) supplemented with 1x protease inhibitor cocktail (Roche, #693116001) and 1x phosphatase inhibitor cocktail (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... After washes, the cells were stained with the DNA stain DAPI (4, 6 diamidino-2-phenylindole dihydrochloride) (Roche, #1023627001) and mounded with Prolong gold anti-fade mound media (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with indicated concentrations of SN-38 or DMSO for 72 h before MTT assay (Roche, 11465007001), according to the manufacturer instructions ...
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bioRxiv - Genetics 2024Quote: ... The testis cells were immediately dissociated through homogenization in 1X PBS containing proteinase inhibitor cocktail (PIC, cOmplete tablets Roche) (PBS+PIC) ...
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bioRxiv - Cancer Biology 2021Quote: ... The let-A and I1B fragments were amplified from S2 cell genomic DNA using the Expand Long Template PCR System (Roche) and cloned in pBacPAK8_EGFP under a metallothionein promoter ...
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bioRxiv - Biochemistry 2020Quote: For Figures 2 and 6: Cells or embryos were lysed in RIPA buffer supplemented with a cocktail of protease inhibitor (Roche). Total protein was resolved on SDS polyacrylamide gels (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were grown for 96 h and cellular viability was determined at indicated time points using the cell proliferation reagent WST1 (Roche) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... Cells were lysed after 24 hours and protein was extracted with RIPA buffer with 1x protease inhibitors EDTA-free (Roche). Protein expression was measured through LICOR Western blotting as described above.
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bioRxiv - Molecular Biology 2020Quote: ... Lentiviral preparations were prepared using standard techniques by co-transfection of 293T cells with the lentiviral construct together with pMDLg/pRRE and pMD2.G using Fugene (Roche). Culture supernatants were harvested at 72 h after transfection and concentrated by ultracentrifugation using standard techniques ...
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bioRxiv - Cell Biology 2020Quote: ... centrifuged and 25 µL of cell-/ debris-free supernatant was used per well for the LDH assay (Roche, Basel, Switzerland).
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bioRxiv - Cell Biology 2020Quote: Expression level of Nud1-HA3 and Nud17A-HA3 was determined by western blot analysis of whole cell extracts as previously described (Ten Hoopen et al., 2012) using monoclonal antibody 12CA5 (Roche) at 1:1000 dilution and monoclonal antibody B-5-1-2 (Sigma ...
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bioRxiv - Cell Biology 2020Quote: Cells were pelleted and lysed with 1.5% n-dodecyl-D-maltoside (DDM) in PBS with cOmplete™ protease inhibitor (Roche) for 15 min on ice ...
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bioRxiv - Cell Biology 2020Quote: ... Cell viability was assessed by addition of 5 μl of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (i.e., MTT) using a Cell Proliferation Kit I (Roche Diagnostics, Mannheim, Germany), in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Cell viability was determined by MTT (C, N-diphenyl-N′-4,5-dimethyl thiazol-2-yl tetrazolium bromide) assay (Roche, Switzerland), a standard colorimetric assay which uses the metabolic reduction of the tetrazolium salt to form the colored formazan product [33] ...
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bioRxiv - Biochemistry 2021Quote: ... or a fixed amount of JR-FL or LAI pseudo-virus (1,000 pg of CA-p24) was added to the cells (70-80% confluency) in the presence of 400 nM saquinavir (Roche) to block secondary rounds of infection and 40 µg/ml DEAE in a total volume of 200 µl ...
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bioRxiv - Developmental Biology 2021Quote: ... The crosslinking reaction was stopped adding Glycine to a final concentration of 0,125 M and the cell pellet was washed three times with cold PBS with protease inhibitors (Complete mini EDTA –free proteinase inhibitor cocktail; Roche). Fixed samples were stored at -80C ...
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bioRxiv - Developmental Biology 2020Quote: Cells were lysed with ice-cold RIPA lysis buffer containing EDTA-free protease inhibitor cocktail and PhosSTOP phosphatase inhibitor (Roche), with DNA-shearing by 21-gauge needle ...
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bioRxiv - Genomics 2020Quote: ... cell pellets of approximately 1-5 million cells digested overnight at 50C in 500 μl lysis buffer containing 100 μg/ml proteinase K (Roche), 10 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: Total RNA from 4 replicates of BCBL-1 WT clone B4 and 57KO clone #6 cells were isolated by TriPure Reagent (Roche) 24 h after induction with VA ...
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bioRxiv - Neuroscience 2022Quote: ... Real time PCR for tuft cell markers and antimicrobial peptide genes was performed using Fast Start Universal SYBR Green Master (Roche) and ViiA™7 Real-Time PCR System (Life Technologies) ...
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bioRxiv - Neuroscience 2021Quote: ... and then washed in PBS buffer before incubation with terminal deoxynucleotide transferase (In Situ Cell Death Detection kit; Roche, Switzerland) for 1 h at 37 °C in a solution containing TMR red dUTP ...