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Citations for Roche :
1701 - 1750 of 6394 citations for Fipronil Sulfone 3 Cyano Pyrazole 3 4 5 13C4 99%; 3 Cyano 5 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Cells were harvested by centrifugation at 7000g at 4°C for 10 minutes and resuspended in 25 mL of cold TBS buffer (50 mM Tris-HCl, 500 mM NaCl, pH 8) with 5% glycerol and EDTA-free protease inhibitors cocktail (Roche). Cells were lysed by sonication and purified using Glutathione-Sepharose 4B (Cytiva ...
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bioRxiv - Neuroscience 2022Quote: ... homogenized on ice in 5 volumes (w/v) of T-per extraction buffer complemented with protease inhibitor tablets (Complete Mini Protease Inhibitor Tablets, Roche) and phosphatase inhibitor cocktail tablets (phosSTOP ...
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bioRxiv - Cell Biology 2022Quote: ... 30 s at 72°C) and final extension 5 min at 72°C using KAPA Taq DNA polymerase (KAPA BIOSYSTEMS). PCR products were visualized on 2% agarose gels containing SYBR Safe DNA gel stain (Invitrogen) ...
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bioRxiv - Biochemistry 2022Quote: ... pH 7.5 and various concentrations of ADP-ribose or hit compounds were mixed on ice in 384-well PCR plate (Roche). Fluorescent signals were measured from 25 to 95 °C in 0.2 °C/30/Sec steps (excitation ...
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bioRxiv - Cell Biology 2022Quote: ... Glycerol was added to a final concentration of 10% as well as Turbonuclease (5 µL) and a protease inhibitor cocktail tablet (Roche). The cells were lysed by sonication (70% amplitude,10 s pulse/10 s pulse off for 2.5 min) ...
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bioRxiv - Molecular Biology 2021Quote: ... un-probed input RNA was also subjected to Sanger sequencing: RNA was dissolved in 5 μl water and supplemented with 1 μl of 10 mM corresponding ddNTP (Roche), then reverse-transcribed into cDNA and analysed by PAGE as described below.
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bioRxiv - Molecular Biology 2021Quote: ... pre-let-7 and pre-miR-16 molecules (miRVana) were radioactively 5’-labeled with 32P isotopes by incubation with 32P-ƴATP and the phosphorylating enzyme T4 PNK (Roche) for 30 min at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... 30 mM imidazole, 1mM DTT, 0.75% CHAPS, 1 μM DNases, 5◻mM MgCl2 and EDTA-free protease inhibitor cocktail [Roche]). Purification was similar as the above instead of the elution buffer used (50 mM HEPES ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then washed 5 times with ice-cold PBS and lysed in RIPA buffer supplemented with cOmplete Protease Inhibitor Cocktail (Roche). Cell lysis was facilitated by sonication and following centrifugation ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked in TBST with 5% milk protein and probed with the following antibodies: 1:1000 anti-HA (Roche), then 1:1500 goat anti-rat IgG-HRP (Dako) ...
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bioRxiv - Neuroscience 2020Quote: ... nt 588-682 from sequence NM_001110222 detected with the mDCX probe (Universal ProbeLibrary probe #68, Roche, Cat.No. 04688678001; 5′-(Fam)-CTGCTCCT-(Dark Quencher)-3′) ...
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bioRxiv - Neuroscience 2020Quote: ... nt 172–244 from sequence NM_011443 detected with the mSOX2 probe (Universal ProbeLibrary probe #63, Roche, Cat.No. 04688627001; 5′-(FAM)-AGGAGGAG-(Dark Quencher)-3′) ...
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bioRxiv - Neuroscience 2020Quote: ... nt 33-140 from sequence NM_016967 detected with the mOLIG2 probe (Universal ProbeLibrary probe #21, Roche, Cat.No. 04686942001; 5′-(Fam)-CAGAGCCA-(Dark Quencher)-3′) ...
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bioRxiv - Neuroscience 2020Quote: ... nt 376-486 from sequence NM_008742 detected with the mNT3 probe (Universal ProbeLibrary probe #49, Roche, Cat.No. 04688104001; 5′-(Fam)-GGCCACCA-(Dark Quencher)-3′) ...
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bioRxiv - Neuroscience 2020Quote: ... nt 225-323 from sequence NM_001244198 detected with the mPAX6 probe (Universal ProbeLibrary probe #67, Roche, Cat.No. 04688660001; 5′-(Fam)-CTCCAGCA-(Dark Quencher)-3′) ...
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bioRxiv - Neuroscience 2022Quote: ... supplemented with 0.5% Nonidet P 40 Substitute (NP40; Fluka Analytical) and cOmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails (Roche)] ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were centrifuged at 300 g for 5 minutes and red blood cells were lysed from samples using RBC lysis buffer (Roche) in 5 ml for 10 minutes at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: Total DNA was isolated from L3 larvae or 5-days old adults of Iduna homozygous mutants and control sequencing strain using the Roche genomic DNA extraction kit (Roche). To confirm the mutant line ...
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bioRxiv - Biochemistry 2019Quote: ... were lysed with lysis buffer (25mM Tris, 150mM NaCl, 1mM EDTA, 1% NP-40, 5% Glyceral) with proteinase inhibitor cocktail (Roche) on ice for 30 min ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... anti-PD-L1 (10 mg/kg for the first dose with each subsequent dose at 5 mg/kg; atezolizumab, Roche), anti-TGF-β (10 mg/kg ...
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bioRxiv - Cancer Biology 2020Quote: ... Nuclei were pelleted at 1350xg for 5 min at 4°C and lysed in 5 mL LB2 (10 mM Tris-Cl pH 8.0, 5 M, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 1 mM PMSF, Roche protease inhibitors) for 10 min at RT with rotation ...
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bioRxiv - Genomics 2020Quote: ... was synthesised commercially and then labelled with digoxigenin-11-2’-deoxyuridine-5’-triphosphate (DIG-11-dUTP) using terminal transferase (Roche) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... They were then incubated with TNB (5% FBS in TNT) for 15 min before incubation with peroxidase-conjugated anti-DIG antibody (DIG-POD, Roche) 1:200 in TNB for 2 hr at RT ...
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bioRxiv - Immunology 2020Quote: The reaction mix per sample for quantitative real-rime PCR (qPCR) contained: 5 µl of the LightCycler 480 SYBR Green I Master Mix (04707516001, Roche), 2.5 µl cDNA and 2.5 µl primers in a total reaction volume of 10 ul ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA quantification by real-time PCR was performed on a 20-fold dilution of the ligation mixture with a QuantStudio 5 system using the LC480 SYBR Green Master mix (Roche) and primers hybridizing in the adapter and Ty1 LTR (Supplementary Table 3) ...
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bioRxiv - Microbiology 2020Quote: ... thus one negative control is placed after every 5 samples on a Light cycler 480 multiwell plates 96-well plate (Roche).
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bioRxiv - Systems Biology 2020Quote: ... supplemented with 0.5% Nonidet P 40 Substitute (NP40; Fluka Analytical) and cOmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails (Roche)] ...
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bioRxiv - Microbiology 2019Quote: ... Each sample was run in triplicate using a 10 μl reaction volume consisting of: 5 μl of CyberGreen Mastermix (Roche), 0.5 μl forward primer (30 μM) ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 0.5% Nonidet P 40 Substitute (NP40; Fluka Analytical) and cOmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails (Roche)] ...
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bioRxiv - Cell Biology 2020Quote: ... anti-SPD-5 (1:1000, a generous gift from B. Bowerman, Hamill et al., 2002) and anti-GFP (1:500, Roche) overnight at 4°C and with secondary antibodies Alexa488 (1:500 ...
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bioRxiv - Molecular Biology 2020Quote: Cells were lysed in lysis buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 1% Nonidet P-40, 1 mM sodium orthovanadate and 1× complete protease inhibitor cocktail from Roche) at 4°C for 15 minutes ...
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bioRxiv - Developmental Biology 2021Quote: Pitx1 WISH were performed on 40-45 somite stage mouse embryos (E12.5) using a digoxigenin-labeled Pitx1 antisense riboprobe transcribed from a cloned Pitx1 probe (PCR DIG Probe Synthesis Kit, Roche), as previously described in (Kragesteen et al ...
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CRISPR-Csx28 forms a Cas13b-activated membrane pore required for robust CRISPR-Cas adaptive immunitybioRxiv - Biochemistry 2021Quote: ... The membrane fraction was resuspended in membrane resuspension buffer (50 mM sodium phosphate buffer, 300 mM NaCl, 5% glycerol, EDTA-free protease inhibitor (Roche), 2% DDM ...
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bioRxiv - Biochemistry 2021Quote: ... a linear DNA tether of the same length was amplified using a digoxigenin-modified primer instead of NTA (5’-DIG-NHS-TCCAAAGGTGAAGAACTGTTCACC, Integrated DNA Technologies, Inc. USA) to bind to anti-digoxigenin Fab fragment antibodies (11214667001, Roche).
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bioRxiv - Biochemistry 2021Quote: Cell extracts were prepared in 1% NP40 lysis buffer (20 mM Hepes pH 7.5, 150 mM NaCl, 1% NP40, 50 mM NaF, 1 mM Na3VO4, 10% glycerol, and protease inhibitor cocktail from Roche) at 4 °C for 15 mins ...
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bioRxiv - Immunology 2021Quote: ... was used for the preparation of sequencing libraries corresponding to surface marker and cytokine while additional part (5 µl) was used for the construction of sequencing library of sample hashtags using KAPA HiFi Master Mix (Roche). The 2nd-round P7/read 2-incorporated PCR primers used for protein library preparation were listed in Table S2 with the P5/read 1-incorporated primer the same to that used in cDNA library ...
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bioRxiv - Biophysics 2021Quote: ... supplemented with 0.5% Nonidet P 40 Substitute (NP40; Fluka Analytical) and cOmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails (Roche)] ...
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bioRxiv - Genomics 2021Quote: ... Nuclei were pelleted by spinning at 500 g for 5 min at 4°C and resuspended in 1 mL cold CUT&RUN wash buffer (20 mM HEPES pH 7.5, 0.2% Tween-20, 150 mM NaCl, 0.1% BSA, 0.5 mM spermidine, 10 mM sodium butyrate, Roche Protease Inhibitor Cocktail). 10 μL concanavalin-coated bead slurry (Bangs Laboratories (Fishers ...
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bioRxiv - Microbiology 2021Quote: ... the bacterial cells were suspended in 20 ml cold buffer A (500mM NaCl, 50mM Tris pH 7.5, 5% glycerol, 10 mM imidazole) supplemented with cOmplete™ protease inhibitor cocktail (Roche), 100 ug/ml lysozyme ...
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bioRxiv - Microbiology 2021Quote: ... Cells pellets were immediately snap frozen on dry ice for about 5 mins and then thawed and prepared for lysis using a Magnalyser (Roche) at 7000 rpm for 1 minute followed by cooling on ice for 4 min ...
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bioRxiv - Genomics 2021Quote: ... PCR products from the 5 reactions were mixed for library preparation using FastStart High Fidelity PCR System dNTPack (Roche, 3553400001) and Nextera XT Index Kit (illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... The reactions were diluted 5 times in water before quantitiative real-based protocol time PCR on a LightCycler LC480 (Roche), using SYBR-based protocol based Mesa Blue qPCR Mastermix (Eurogentec ...
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bioRxiv - Biochemistry 2022Quote: ... 0.1 mM ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA) and 5% (v/v) glycerol and Supplementarylemented with a protease inhibitor cocktail tablet and a phosphatase inhibitor tablet (Roche). Lysates were briefly sonicated on ice and clarified by centrifuged at 20,817g for 20 min at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... N’-tetraacetic acid (EGTA) and 5% (v/v) glycerol and supplemented with a protease inhibitor cocktail tablet and a phosphatase inhibitor tablet (Roche). Lysates were briefly sonicated on ice and clarified by centrifuged at 20,817g for 20 min at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Wells were then washed twice with PBT 0.2% and blocked with PBT 0.2% containing 5% bovine serum albumin (Roche, Cat# 10735094001). Primary antibody staining with anti-Wg (1:500 ...
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bioRxiv - Genetics 2022Quote: ... 50000 cells were lysed in NPB buffer (PBS containing 5% BSA, 1mM DTT, 0.2% IGEPAL, 1□×_Roche Complete Protease Inhibitor) and incubated on ice for 15 min to isolate nuclei ...
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bioRxiv - Molecular Biology 2022Quote: ... were co-transfected with SAE2-T2A-SAE1 cDNA in the pcDNA5/FRT/TO vector and the Flp-recombinase cDNA in the pOG44 vector at a 5:1 pcDNA5/FRT/TO DNA: pOGG44 DNA ratio using FuGene6 (Roche) at a ratio of 4:1 FuGene (μl) ...
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bioRxiv - Biochemistry 2022Quote: ... or octamer-mix were done at 900 MHz 1H Larmor frequency at 298 K in NMR buffer (25 mM NaPi, pH 7, 5% D2O, with 1x protease inhibitors (complete EDTA-free cocktail, Roche)) with 600 mM NaCl for the titrations with H2A-H2B and H3-H4 and 300 mM NaCl for titration with octamer-mix ...
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bioRxiv - Plant Biology 2022Quote: ... The abundance of specific transcript was measured by probing 1 μL cDNA by quantitative real-time PCR in a total volume of 10 μl containg 5 μL SYBR-green master mix (Roche), 0.5 μM forward and reverse primer and water ...
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bioRxiv - Cancer Biology 2022Quote: ... 2-10 5 micron sections were extracted using the High Pure FFPE RNA Isolation kit (Roche Life Sciences, Penzberg, Germany) under RNase free conditions following the manufacturer’s protocol ...