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Citations for Roche :
1501 - 1550 of 6394 citations for Fipronil Sulfone 3 Cyano Pyrazole 3 4 5 13C4 99%; 3 Cyano 5 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... 50 mM Tris-HCl pH 8.0, 5 mM MgCl2, 0.25% IGEPAL CA-630, 1 mM DTT, cOmplete protease inhibitor [Roche]) and mixed with 200 µL of 3 µg µL−1 nuclear extract and 500 µL PBB+ buffer ...
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bioRxiv - Cell Biology 2022Quote: ... 50 mM Tris-HCl pH 8.0, 0.25% IGEPAL CA-630, 1 mM DTT, 5 mM MgCl2, cOmplete protease inhibitor by Roche). Per replicate and construct ...
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bioRxiv - Cell Biology 2022Quote: ... 50 mM Tris-HCl pH 7.5, 0.5% IGEPAL CA-630, 5 mM MgCl2, 1 mM DTT, 1x protease inhibitor EDTA free [Roche]) and mixed with 50 µL PBB-buffer-equilibrated MyOne Streptavidin C1 Dynabeads (Thermo Scientific) ...
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bioRxiv - Biophysics 2022Quote: ... 200 mM NaCl, 5 mM Beta glycerophosphate, 0.1 mM sodium orthovanadate, 2 mM TCEP, 0.4% NP40, 1X Roche EDTA free mini complete protease inhibitor) ...
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bioRxiv - Genetics 2023Quote: Quantitative RT-PCR was performed in a final volume of 5 μl with SYBR Green I Master (Roche) and analyzed with a Lightcycler 480 (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... Beads were washed three times with 200 µL of washing buffer (50 mM Tris-HCl at pH 7.5, 150 mM KCl, 5 mM MgCl2, 0.05% NP-40, EDTA-free cOmplete mini protease inhibitor [Roche, 11836153001] ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA extracted from the parasites at passages 7 and 20 were digested with 5 U Rsa1 (Roche) at 37 °C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... Bacterial pellets were collected via centrifugation at 4°C and 5,000g and resuspended in ice-cold lysis buffer (50 mM MES pH 6.8, 1 mM EGTA, 0.2 mM MgCl2, 5 mM DTT, Roche complete protease inhibitor ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed twice with PBS and lysed for 20 min on ice in lysis buffer (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 5 mM EDTA, 0.5% NP-40, supplemented with protease inhibitors [Roche]). Centrifugation at 13,000×g for 10 min at 4°C was then used to remove cell debris ...
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bioRxiv - Microbiology 2023Quote: ... after which they were resuspended in 1.0 ml of ice-cold lysis buffer (10 mM Tris-HCl, pH 7.7; 10 mM NaCl; 5 mM MgCl2; 0.1 mM EGTA; protease inhibitors [Roche]) and incubated on ice for 10 min before nuclei were pelleted (400 × g ...
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bioRxiv - Molecular Biology 2024Quote: ... Pellets were resuspended in 500 µL cold cell lysis buffer (5 mM PIPES pH8.0, 85 mM KCl, 0.5% NP-40, 1x protease/phosphatase inhibitor, Roche) and lysed (10 min on ice) ...
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bioRxiv - Molecular Biology 2024Quote: ... 400 mM NaCl, 1.5 mM MgCl2, 0.2 mM EDTA, 1 mM DTT, 5% glycerol, 1x protease/phosphatase inhibitor cocktails, Roche). Nuclear lysates were diluted with 2 volumes of dilution buffer (20 mM HEPES pH7.9 ...
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bioRxiv - Pathology 2023Quote: Human normal and IPF lungs were minced with a razor blade in a 100 mm petri dish in a cold DMEM medium containing 0.2 mg/ml Liberase DL and 100 U/ml DNase I (Roche, Indianapolis, IN, USA). The mixture was transferred into 15 ml tubes and incubated at 37 °C for 35 min in a water bath under continuous rotation to allow enzymatic digestion ...
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bioRxiv - Pathology 2023Quote: ... The lungs were immediately harvested and minced with a razor blade in a 100 mm petri dish in a cold DMEM medium containing 0.2 mg/ml Liberase DL and 100 U/ml DNase I (Roche, Indianapolis, IN, USA). The mixture was transferred into 15 ml tubes and incubated at 37 °C for 35 min in a water bath under continuous rotation to allow enzymatic digestion ...
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bioRxiv - Developmental Biology 2019Quote: ... These fragments were pooled and dissociated using 1:100 Liberase™ (2.5mg/ml, Roche 5401135001) in CMFB at 30°C with gentle agitation at 300 rpm for 30 minutes on a ThermoMixer™ Eppendorf Mixer ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then treated using 50□μl of 100□mg/ml lysozyme (Roche Molecular Systems). Furthermore ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were cultured as normal and incubated in 100 ng ml−1 Colcemid (Roche, MA) for 2 hours prior to harvest ...
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bioRxiv - Immunology 2023Quote: ... Tumors were cut into small pieces and incubated with Liberase (100 µg/ml, Roche #5401020001) and DNAse I (50 µg/ml ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... harvested lungs were minced and digested with Liberase TM (100 µg/mL; Roche, Indianapolis, IN), collagenase XI (250 µg/ml) ...
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bioRxiv - Cancer Biology 2019Quote: ... each well was treated with enzyme mixture: 750 µl Collegenase/Dispase 4 mg/ml (Roche, #10269638001), 30 mg BSA (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: Embryos were incubated in 1μg/ml of 4′,6-diamidino-2- 532 phenylindole (Roche, Cat# 10236276001) at RT for 10min and washed in PBS 1x ...
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bioRxiv - Immunology 2022Quote: ... were coated O/N at 4 °C with 50 μl of fibronectin (50 μg/ml; Roche) or VCAM-1-Fc (10 μg/ml R&D System) ...
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bioRxiv - Microbiology 2020Quote: ... IFAs were carried out on methanol-fixed cells using primary antibodies mouse mAb α-GFP (Roche Diagnostics #11814460001), 1:100 and rabbit α-PfHP1 12 ...
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bioRxiv - Neuroscience 2023Quote: ... were activated in methanol for 30 seconds and then incubated with transfer buffer (3.02 g Tris (Roche, 107089176009), 14.4 g glycine (Sigma ...
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bioRxiv - Genetics 2021Quote: ... with 3x protease inhibitors (cOmplete Protease Inhibitor Cocktail EDTA-free - 1 mM phenylmethylsulfonyl fluoride, 4 mM benzamidine, 2 μg/ml leupeptin, and 1 μg/ml pepstatin, Roche Diagnostics, cat. number 1187358001) and 3x phosphatase inhibitors (Millipore Inhibitor Cocktail Set ...
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bioRxiv - Physiology 2022Quote: ... This was followed by a perfusion at 4 mL/min for 40 min with the same solution containing 1 mg/mL of collagenase A (Roche Diagnostics GmbH, Mannheim, Germany) plus 300 µM ethylene glycol tetraacetic acid (EGTA ...
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bioRxiv - Cancer Biology 2021Quote: ... one 10 cm plate of Hepa1c1c7 cells at 80% confluence was lysed in 1 mL Pierce IP Lysis Buffer (supplemented with 100 U/mL SUPERase-IN and 1X Roche protease inhibitor cocktail) and incubated on ice for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... 10 mM NaCl, 1.5 mM MgCl2, 1 mM DTT, 100 μg/ml PMSF, 1 μg/ml pepstatin, and Roche Complete Protease Inhibitor Cocktail) with 100 U/ml of OmniCleave nuclease (Epicentre ...
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bioRxiv - Microbiology 2024Quote: ... 1.5 mM MgCl2, 0.1% IGEPAL Nonidet P-40, 1 mM DTT, 100 μg/ml PMSF, 1 μg/ml pepstatin, and Roche Complete Protease Inhibitor Cocktail) and transferred to a Dounce homogenizer and homogenized with 20 slow strokes ...
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bioRxiv - Cell Biology 2021Quote: ... the sections were incubated overnight at 4 °C in digoxigenin antibodies conjugated to horseradish peroxidase (anti-digoxigenin-POD; Fab fragment; 1:100; Roche), rinsed in TBS (0.1 M Tris-HCl with 0.9% NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... washed with PBS and resuspended in lysis buffer (0.5% Triton X-100, 4% w/v SDS, 0.5xPBS) supplemented with protease inhibitor cocktail (Roche, Basel, Switzerland). The lysates were then resuspended in 5 x SDS-PAGE loading buffer containing 25mM DTT ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were harvested using ice cold EBC buffer (140 mM NaCl, 50 mM Tris-HCl, 0.5% NP-40, 5 mM EDTA and protease inhibitor cocktail (Roche)) and briefly sonicated prior to 20 min centrifugation at 20,000 g ...
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bioRxiv - Molecular Biology 2021Quote: Mouse livers were homogenized with a motorized tissue grinder in STM buffer (Tris-HCl pH 7.4, 5 mM MgCl2, 250 mM Sucrose, 1 mM DTT, supplemented with 500 µM PMSF, Protease Inhibitor Cocktail [Roche, Basel ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were lysed in 500 μl lysis buffer (50mMTris-HCl, pH8.0, 100mM NaCl, 10m MEDTA, 5% glycerine, 1% NP- 40 and protease inhibitor cocktail (Roche)) for 40 min at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 mM NaCl, 5 mM MgCl2, 0.5% NP-40, 1 mM DTT, and 1x cOmplete EDTA-free protease inhibitor cocktail [Roche]), washed once with PBS ...
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bioRxiv - Cell Biology 2022Quote: ... 5 mM EDTA) with the addition of a Complete EDTA free protease inhibitor cocktail tablet (Roche Diagnostics, Oslo, Norway) and a PhosSTOP tablet (Roche) ...
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bioRxiv - Neuroscience 2019Quote: ... cells were collected and lysed with a lysing buffer (100mM KCl, 20mM HEPES, 5% Glycerol, 0.1X Triton-X, 1mM DTT, and Complete protease inhibitor (Roche)) ...
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bioRxiv - Cell Biology 2019Quote: ... 2 mM MgCl2, 1 mM EGTA, 0.1% [v/v] NP-40, 1 mM DTT, 5% [v/v] glycerol, Roche Complete Protease Inhibitors and PhosSTOP Protein Phosphatase Inhibitors ...
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bioRxiv - Neuroscience 2019Quote: ... Resin was washed with TBS buffer (150 mM NaCl, 20 mM Tris-Cl, pH 7.4, 5 mM MgCl2, EDTA free protease inhibitors (Complete, Roche) and incubated with purified 6xHis-tag LC3 for 120 min @4°C in TBS buffer either alone or in the presence of purified 6xHis-Snapin ...
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bioRxiv - Neuroscience 2020Quote: ... Each tissue sample was dissociated using 2.4 mL of homogenization buffer (10 nM Tris pH 8, 5 mM MgCl2, 25 mM KCl, 250 mM sucrose, 1 μM DTT, 0.5x protease inhibitor [cOmplete, Roche #4693159001] ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 mM KCl, 5 mM MgCl2, 0.01% NP-40, freshly prepared 20 mM Na2MoO4, and cOmplete protease inhibitors; Roche). 10-20 million cells were lysed in 0.5 mL Felts buffer by rotation at 4°C for 30 minutes and vortexing for 10 seconds ...
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bioRxiv - Plant Biology 2021Quote: ... Each 10 μl reaction was comprised of 5 μl LightCycler 480 SYBR Green I Master mix (Roche Life Science), 2 μl cDNA template ...
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bioRxiv - Biochemistry 2021Quote: ... Pelleted cells were resuspended in 1x PBS buffer (pH 7.4) with 5 mM BME and proteinase inhibitor cocktail (Roche). For purification of PABPC1-His ...
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bioRxiv - Biochemistry 2020Quote: ... re-suspended with buffer A (50mM Phosphate buffer pH 8, 400mM NaCl, 5% glycerol, 1mM DTT, 0.5mM EDTA, protease inhibitor cocktail from Roche) and lysed using a microfluidizer followed by two cycles of centrifugation (12000 × g 20 min) ...
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bioRxiv - Microbiology 2020Quote: ... cells were centrifuged and pellets were resuspended in RB1 (20mM Tris pH7.5, 300mM NaCl, 1mM DTT, 5% glycerol and protease inhibitor (complete EDTA-Free, Roche)) ...
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bioRxiv - Plant Biology 2020Quote: ... 0.5 mM PMSF) supplemented with 5 mg L−1 DNAse I and 0.5 g/L lysozyme (Roche, Mannheim, Germany). Cells were disrupted by sonication (3×2 min ...
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Plant pathogens convergently evolved to counteract redundant nodes of an NLR immune receptor networkbioRxiv - Plant Biology 2021Quote: ... 5% (vol/vol) glycerol and 20 mM imidazole (buffer A) supplemented with cOmplete EDTA-free protease inhibitor tablets (Roche) and lysed by sonication ...
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bioRxiv - Plant Biology 2021Quote: ... 500 nM of each primers and 5 μl of 2X LightCycler® 480 SYBR Green I Master mix (Roche) in 10 μl final volume ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was diluted 5 times and subjected to real-time PCR using LightCycler 480 SYBR Green I Master (Roche) with specific primers for the genes of interest ...
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bioRxiv - Microbiology 2021Quote: ... 60 mM KCl, 1 mM CaCl2, 5 mM MgCl2, 300 mM sucrose, 0.4% NP-40, 1 mM DTT, 1x Roche Complete protease inhibitors cocktail EDTA-free ...