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Citations for Roche :
1601 - 1650 of 6394 citations for Fipronil Sulfone 3 Cyano Pyrazole 3 4 5 13C4 99%; 3 Cyano 5 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Hematocrit (0.5 to 0.64) and blood glucose concentration (5.7 to 8.6 mmol/L, Accu-Chek Performa Nano, Roche, Mannheim, GER) were measured shortly before injecting the radiotracer.
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bioRxiv - Molecular Biology 2023Quote: ... Pellets were resuspended in microsome buffer (50 mM MOPS, 0.5 M sorbitol, 5 mM EGTA, complete protease inhibitor (Roche)) and repelleted by centrifugation at 100□000 g for 30 min at 4°C ...
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bioRxiv - Genomics 2023Quote: ... resuspended in cold lysis buffer (10 mM Tris, pH 7.5, 10 mM NaCl, 5 mM MgCl2, 0.1 mM EGTA, Protease Inhibitor (Roche, 04693159001)) and incubated on ice for 10’ to isolate the cell nuclei ...
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bioRxiv - Genomics 2023Quote: ... Cells were centrifuged at 500 x g for 5 min at 4C and 500 uL 1X PBS + 1% BSA + DAPI + 0.5U/uL Protector RNAse Inhibitor (Roche) was added ...
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bioRxiv - Cell Biology 2023Quote: ... 500 mM NaCl, 0.4% [wt/vol] SDS, 5 mM EDTA, 1 mM DTT and 1x cOmplete Protease inhibitor cocktail, Roche) for 20 min on a rocker at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... 50 mM NaCl, 5 mM EDTA, 50 mM Tris at pH 7.4 and cOmplete EDTA-free Protease Inhibitor Cocktail [Roche]). After agitation at 4°C for 30 min ...
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bioRxiv - Immunology 2023Quote: ... These cells were cultured with healthy donor T cells (E:T ratio of 5:1) and different concentrations of Mosunetuzumab (Genentech, Roche): 0 ...
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bioRxiv - Biochemistry 2023Quote: ... Mitochondrial pellets were solubilized in lysis buffer with 1 % digitonin (25 mM HEPES pH 7.5, 150 mM NaCl, 5 % glycerol, 1 mM TCEP, supplemented with PhosStop (Roche), cOmplete protease inhibitor cocktail EDTA-free (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 mM EDTA, 20 mM HEPES, 0.2% NP-40, 10% glycerol, 400U Ribolock inhibitor, 1x protease/phosphatase inhibitor, Roche). Lysates were centrifuged (12000 rpm ...
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bioRxiv - Genomics 2023Quote: ... Library construction was performed on the 5 ng of pooled PCR amplicons using the KAPA HyperPrep Kit (Roche 07962363001) and NEXTFLEX Unique Dual Index Barcodes (7.5µM final concentration ...
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bioRxiv - Biochemistry 2023Quote: Cell pellets were snap frozen in Eppendorf tubes and lysed with glass beads in 500 μl GFP pull-down buffer (20mM HEPES pH 7.4, 150mM KOAc, 5% glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free, Roche) using a FastPrep (MP biomedicals) ...
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bioRxiv - Biochemistry 2023Quote: Cell pellets were snap frozen in Eppendorf tubes and lysed with glass beads in 500 μl GFP pull-down buffer (20mM HEPES pH 7.4, 150mM KOAc, 5% glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free ...
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bioRxiv - Neuroscience 2023Quote: ... cells and sections were processed for BrdU immunodetection following the manufacturer’s recommendations (5-Bromo-2′-deoxy- uridine Labelling and Detection Kit I, Roche). The slides were mounted for fluorescent microscopy in ProLong® Diamond Antifade Mountant with DAPI (Life Technologies).
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bioRxiv - Biochemistry 2023Quote: ... and individually transferred in 1.5 ml vials filled with 5 volumes (V/W) of ice-cold 25 mM Hepes pH=7.4 in PBS completed with protease (Mini, Roche) and phosphatase (Phos-stop ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed in with glass beads in 500 µl SPOT PD buffer (20mM HEPES pH 7.4, 150mM KOAc, 5% Glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free, Roche) using a FastPrep (MP biomedicals) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed in with glass beads in 500 µl SPOT PD buffer (20mM HEPES pH 7.4, 150mM KOAc, 5% Glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free ...
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bioRxiv - Microbiology 2023Quote: ... Each 5 μl reaction contained 2.5 μl of LightCycler 480 SYBR Green I Master mix 2X (Roche Applied Science), 0.5 μl of each primer (300 nM final ...
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bioRxiv - Microbiology 2023Quote: ... 0.5 mM EDTA, 0.1 % NP-40, 20 mM KCl, 150 mM NaCl, plus a protease inhibitor cocktail from Roche) and centrifuged for 2 minutes and 2,500 g at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: A 20% w/v homogenate was prepared with a piece of snap-frozen liver tissue in homogenization buffer (50 mM Tris-HCl [pH 7.4], 250 mM sucrose, 25 mM KCl, 5 mM MgCl2, 3mM imidazole, supplemented with protease inhibitors [Roche]). To remove nuclei and debris ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed with 500 μl acid-washed glass beads in 500 μl GFP-Trap pulldown buffer (20 mM HEPES, pH 7.4, 150 mM KOAc, 5% glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed with 500 μl acid-washed glass beads in 500 μl GFP-Trap pulldown buffer (20 mM HEPES, pH 7.4, 150 mM KOAc, 5% glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free, Roche) using a FastPrep (MP biomedicals) ...
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bioRxiv - Molecular Biology 2023Quote: ... The pellet was thawed in an equal volume of lysis buffer (1M NaCl, 25mM Tris pH7,5, 5% Glycerol,1mM DTT, protease inhibitors (Roche)) and sonicated on ice (10 pulses of 10 seconds) ...
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bioRxiv - Molecular Biology 2023Quote: ... The pellet was thawed in two volumes of lysis buffer (3M NaCl, 100mM Tris pH7,5, 10% Glycerol, 0.5mM EDTA, 5 mM β-mercaptoethanol, protease inhibitors (Roche)) and sonicated on ice (10 pulses of 10 seconds) ...
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bioRxiv - Synthetic Biology 2023Quote: ... The amplification of the oligonucleotide pool was performed with ∼5 ng of template DNA and KAPA HiFi polymerase (Roche) with the annealing temperature at 53°C and extension time of 15 sec for 14-20 cycles ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and 1X Triton buffer (100mM Tris-HCl, pH8.8, 100mM NaCl, 5mM EDTA and 5% Triton-X) with protease inhibitors (11836170001, Roche) for 16 hours at 4° C ...
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bioRxiv - Biochemistry 2023Quote: ... under gravity flow, washed with PER2 buffer (1 x TBS pH 7.5, 1mM EDTA, 5% glycerol, EDTA-free protease inhibitors (Roche)) and eluted with 3x flag peptide in PER2 buffer ...
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bioRxiv - Microbiology 2023Quote: ... and resuspended in lysis buffer (50 mM PBS pH 7.4, 1 mM EDTA, 5% glycerol, 1 mM PMSF and protease inhibitors from Roche). Cell lysis and protein extraction was achieved through mechanical lysis using glass beads and Precellys®24 Homogenizer (3 cycles of 30 seconds at 5500 rpm ...
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bioRxiv - Cell Biology 2023Quote: ... Protein was purified from these cells by freeze-thaw lysis in Hepes buffer (20mM Hepes; pH 7.5, 150mM NaCl, 5% glycerol) with Complete protease inhibitor tablets (Roche) or Phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Genetics 2023Quote: ... the cell pellet was resuspended in 750 μl Tris lysis buffer (50 mM Tris, 0.2% NP-40, 150 mM NaCl, 5 mM EDTA, 5.7 mM PMSF, and 1 x Roche cOmplete). For whole organ samples (either whole detunicated testis or thymus) ...
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bioRxiv - Biochemistry 2023Quote: ... The pellet was thawed in two volumes of lysis buffer (3M NaCl, 100mM Tris pH7,5, 10% Glycerol, 0.5mM EDTA, 5 mM β-mercaptoethanol, protease inhibitors (Roche)) and sonicated on ice (10 pulses of 10 seconds) ...
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bioRxiv - Developmental Biology 2024Quote: ... heads were rinsed for 5 minutes in PBST five times and incubated in Blocking Reagent (Roche, 1:5000 dilution) for 60 minutes ...
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bioRxiv - Cell Biology 2024Quote: Cells or tissues were washed with PBS and lysed in an appropriate amount of protein lysis buffer (TritonX100 -1%, Tris 7.4 -50mM, EDTA -5mM, NaCl -150mM, and glycerol -5%) containing Protease Inhibitor Cocktail (Roche). We used Phosphatase Inhibitor Cocktail (Millipore ...
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bioRxiv - Neuroscience 2024Quote: ... .5 µL of a 10 µM primer mix and 7.5 µL of Fast Start SYBR Green Master Mix (Roche). Each reaction was performed in triplicate ...
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bioRxiv - Physiology 2024Quote: ... Sections were dried and denatured for 10 min at 80°C in hybridization buffer (0.1M Tris, pH 7.2, 25mM MgCl2, 70% formamide [Sigma-Aldrich, Saint Louis, MO], 5% blocking reagent [Roche] with 1.0 μg/mL of Cy-3-labeled telomere-specific [CCCTAA] peptide nucleic acid [PNA] probe [TelC-Cy3 ...
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bioRxiv - Developmental Biology 2024Quote: ... 5 ng amplified DNA and 1 μM forward and reverse primers and run on a LightCycler 480 machine (Roche) using the following protocol ...
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bioRxiv - Neuroscience 2024Quote: ... The template plasmid was diluted to 5×107 copies/µL with nuclease-free water plus yeast RNA (Roche, 10109223001), and this solution was further diluted to 2.5 ...
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bioRxiv - Neuroscience 2024Quote: ... 5-diphenyl tetrazolium bromide (MTT) in accordance with the manufacturer’s recommendations (Cell Proliferation Kit I; Roche Diagnostics, Basel, Switzerland). In brief ...
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bioRxiv - Molecular Biology 2024Quote: ... 150 mM NaCl, 0.5% NP40, 0.5% Triron-X100, 5% glycerol, 1x Roche Complete EDTA free inhibitor cocktail, 1x Roche PhosSTOP) for 1h on ice by agitation ...
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bioRxiv - Microbiology 2024Quote: ... with their respective dilution in 5% skimmed milk in PBS-tween 0.1% were used: anti-HA-HRP (3F10) (N°12013819001, Roche) 1/10,000 ...
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bioRxiv - Cell Biology 2024Quote: ... containing 5% beta-mercaptoethanol and protease and phosphatase inhibitors (Roche Complete Mini protease inhibitor, Sigma-Aldrich #11836153001, and Roche Complete Ultra phosphatase inhibitor ...
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bioRxiv - Plant Biology 2023Quote: ... Samples were then ground to a fine powder in a mortar and pestle over liquid nitrogen and resuspended in freshly prepared extraction buffer (2mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% v/v NP40, 5% v/v glycerol, 1mM PMSF, 1ml Roche Protease Inhibitor cocktail per 30g input tissue ...
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bioRxiv - Neuroscience 2024Quote: ... underwent lysis through the addition of 200 µl of a 50 mM Tris-HCl buffer (pH 7.8) containing 5% SDS and cOmplete ULTRA protease inhibitor (Roche). The samples were then subjected to Bioruptor® treatment (Diagenode ...
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bioRxiv - Developmental Biology 2022Quote: Paralarval brains were enzymatically dissociated by adding 20 µl of Collagenase/Dispase (100 mg/ml, Roche) to 500 µl L15-adapted medium and incubating for two hours at 25°C ...
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bioRxiv - Cell Biology 2021Quote: ... Eluted materials were transferred to new tubes and treated with RNase A (100 μg/ml; Roche) for 2.5 hours at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... Glutamax and Pen/Strep and with 100 U/mL recombinant human IL-2 (Roche; Sigma # 10799068001). For the analysis of reverse transcription products ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were cultured in RPMI + IL-2 (final concentration 100 U/mL, Roche, 10799068001), IL-7 (final conc ...
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bioRxiv - Pathology 2021Quote: ... Then hearts were perfused and digested with perfusion buffer plus 0.067 mg/ml Liberase Blendzyme 4 (Roche) for 30min ...
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bioRxiv - Cancer Biology 2023Quote: ... + 4% fetal bovine serum (FBS, Hyclone) and 0.01 mg/mL insulin-transferrin-sodium-selenite solution (ITSS; Roche) as previously described 28,29 ...
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bioRxiv - Immunology 2020Quote: ... and a 30-gauge needle was used to inflate each pancreas through the common bile duct with 4 mL of media supplemented with 0.8 mg/mL of collagenase P (Roche, cat. number 11-249-002-001) and filtered at 0.22 μm prior to injection ...
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bioRxiv - Pathology 2020Quote: ... Coverslips were incubated overnight at 4 °C with a 1:100 dilution with one of the following primary antibodies: HA (Roche, #867423001), KDEL (Abcam ...