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Citations for Roche :
1551 - 1600 of 2327 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: TUNEL assay was carried out following the manufacturer’s instructions (In Situ Cell Death Detection Kit, Roche-11684795910).
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bioRxiv - Neuroscience 2022Quote: ... Transient transfection was achieved by transfecting cells with plasmid DNAs using XtremeGene HP DNA Transfection reagent (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: Cell lysates were prepared in radioimmunoprecipitation assay buffer supplemented with protease inhibitor cocktail tablets (Roche, Basel, Switzerland), and total protein was measured using a Bicinchoninic Acid Protein Assay Kit (Beyotime Biotechnology ...
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bioRxiv - Cancer Biology 2022Quote: Cells were harvested and lysed using the RIPA lysis buffer supplemented with protease inhibitor cocktail (Roche, 04693124001). The frozen tumor tissues were homogenized with RIPA lysis buffer (with protease inhibitor ...
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bioRxiv - Cell Biology 2023Quote: ... and the cell pellet was resuspended in 150 μl of the hypotonic Cell Fraction Buffer (10 mM HEPES, 1.5 mM MgCl2,10 mM KCl, pH 7.9 plus PIM (Roche)) ...
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bioRxiv - Immunology 2023Quote: Cell pellet or tissue were lysed in RIPA lysis buffer containing 1× proteinase inhibitor cocktail (Roche, 5056489001), 2 mM PMSF and 1 mM DTT ...
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Reduction of Spermine Synthase Suppresses Tau Accumulation Through Autophagy Modulation in TauopathybioRxiv - Neuroscience 2023Quote: ... On the fifth day, harvest the cells with RIPA buffer (R0278, Thermo) with proteinase inhibitors (11836170001, Roche) and phosphatase inhibitors (04906837001 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... fixed cells were treated with 333 µL PBS supplemented with 1 µL RNAse (10 mg/mL) (Roche) at 37°C for 30 min ...
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bioRxiv - Biochemistry 2023Quote: Resuspended cells were homogenized by gentle stirring and supplemented with an EDTA-free protease inhibitor cocktail (Roche). The cell suspension was passed three times through an M-110 PS microfluidizer (Microfluidics ...
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bioRxiv - Biochemistry 2023Quote: Resuspended cells were homogenized by gentle stirring and supplemented with an EDTA-free protease inhibitor cocktail (Roche). The cell suspension was passed three times through an M-110 PS microfluidizer (Microfluidics ...
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bioRxiv - Biochemistry 2023Quote: ... Cells (1×105) were then trypsinized and plated on collagen pre-treated cover glasses (Roche Applied Sciences) followed by treatment with GO or Bleo ...
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bioRxiv - Molecular Biology 2023Quote: ... the LDH present in cell-free supernatants was detected in triplicates using the Cytotoxicity Detection kit (Roche) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... gRNA constructs were transfected into HeLa S3 cells (https://www.atcc.org/products/ccl-2.2) with X-tremeGENE 9 (Roche) overnight ...
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bioRxiv - Microbiology 2023Quote: ... and the cell suspension were digested in HBSS containg 25 μg /ml of DNAse I (#11284932001, Roche) and 50 μg/ml Liberase (#5401119001 ...
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bioRxiv - Neuroscience 2023Quote: ... in a PBS-based cell lysis buffer complete with EDTA-free protease inhibitors (#04693159001; Roche, Mississauga, Canada) and 1 mmol/L phenylmethylsulfonyl fluoride (#PMSF123.5 ...
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bioRxiv - Cell Biology 2023Quote: SA2 cDNAs were cloned directly from HCT116 cells by PCR using KAPA HiFi HotStart PCR kit (Roche) (Fwd ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed with 1 ml lysis buffer (0.5 % Nonident-P40, 2 % protease inhibitor cocktail (Roche, Switzerland) and 1 % phosphatase inhibitor cocktails I and II (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: The viable cell density (VCD) and viability were measured using a Cedex HiRes Analyzer (Roche, Basel, Switzerland) from daily samples ...
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bioRxiv - Biophysics 2023Quote: ... and harvested cell pellets resuspended in 250 μl lysis buffer containing a complete protease inhibitor cocktail (Roche). After centrifugation (15,000 × g for 5 min at 4°C) ...
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bioRxiv - Genomics 2023Quote: Cell-free fDNA and gDNA libraries were constructed using the Kapa Hyper Prep kit (cat: 07962363001, Roche) with custom dual index duplex UMI adapters (IDT) ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were lysed by scrapping in RIPA buffer supplemented with EDTA-free protease inhibitors (Roche, cat. # 04693159001). Lysed cells were centrifuged at maximal speed for 10 min ...
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bioRxiv - Cell Biology 2023Quote: ... gRNA constructs were then sequence-verified and transfected into penta KO cells with X-tremeGENE 9 (Roche) overnight and single cells that were positive for GFP were sorted by fluorescence activated cell sorting (FACS ...
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bioRxiv - Molecular Biology 2023Quote: ... 200 µL of cell culture supernatant was collected and the Cytotoxicity Detection Kit (LDH) (Roche, Indianapolis, IN) was then utilized to detect LDH levels in the culture supernatant ...
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bioRxiv - Microbiology 2022Quote: Antigen retrieval was conducted using a Tris based buffer-Cell Conditioning 1 (CC1)-Catalog # 950-124 (Roche). The SARS-CoV-2 spike primary antibody was of rabbit origin ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell-free supernatants were collected and assessed via LDH release assay with a Cytotoxicity Detection Kit (Roche). The percent LDH release was calculated according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... tissue was finely minced and cell dissociation was performed using a mixture of liberase TH (Roche, 05401135001) and DNase-I (Roche ...
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bioRxiv - Microbiology 2023Quote: ... Cell pellets were resuspended in sodium phosphate buffer (PBS) with a Complete Protease Inhibitor Cocktail Tablet (Roche) and lysed using French press at 10,000 psi ...
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bioRxiv - Molecular Biology 2023Quote: Cell lysates were prepared in 1x PXL lysis buffer supplemented with complete protease inhibitors (Roche, cat. #11873580001). All lysates were prepared from equal cell numbers per condition ...
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bioRxiv - Cancer Biology 2023Quote: The cell pellets originating from primary hepatocytes were lysed in cold HEN Buffer supplemented with PhosSTOP (Roche) and cOmplete™ ...
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bioRxiv - Cell Biology 2023Quote: ... washed with PBS and permeabilised at 1 × 107 cells/ml in MELB with cOmplete protease inhibitors (Roche) and 0.025% digitonin (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids were transiently transfected in HeLa cells for 48 hours using X-tremeGENE 9 DNA reagent (Roche). For silencing experiments ...
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bioRxiv - Microbiology 2023Quote: Cell lysates were prepared in lysis buffer containing 1% Nonidet P-40 and protease inhibitor cocktail (Roche) (Cao ...
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bioRxiv - Physiology 2023Quote: ... cells/tissue were lysed in mammalian protein extraction reagent (M-PER) supplemented with protease inhibitor cocktail (Roche). Soluble proteins and insoluble matrix were separated by centrifugation at 10,000 × g ...
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bioRxiv - Immunology 2023Quote: Cells were lysed with RIPA complete lysis buffer supplemented with EDTA-free protease and phosphatase inhibitors (Roche) and 25 U of Benzonase Nuclease ...
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bioRxiv - Immunology 2023Quote: ... Cells were lysed with lysis buffer (500 μL) containing the complete protease inhibitor cocktail (Roche, cat#11697498001). The biotinylated surface proteins were excluded with NeutrAvidin agarose gel (Pierce ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were lysed in RIPA buffer supplemented with protease and phosphatase inhibitors (mini cOmplete and phosSTOP, Roche) on ice for 10 min.
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bioRxiv - Molecular Biology 2024Quote: Harvested cell pellets were lysed in 1xRIPA buffer supplemented with 1x EDTA-free cOmplete protease inhibitor (Roche) and 1x PhosphoSTOP phosphatase inhibitor (Roche ...
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bioRxiv - Cell Biology 2024Quote: Cells were lysed in reducing Laemmli SDS sample buffer containing PhosSTOP (Phosphatase Inhibitor Cocktail Tablets, Roche, Switzerland) at 96°C for 10 min and the proteins separated on NuPAGE® Novex® 4–12% Bis–Tris Gels ...
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bioRxiv - Cell Biology 2023Quote: HeLa cells were lysed with lysis buffer (0.5% Triton X-100 in PBS) containing protease inhibitors (Roche) for 15 min on ice ...
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bioRxiv - Immunology 2024Quote: Antigen retrieval was conducted using a Tris based buffer-Cell Conditioning 1 (CC1)-Catalog # 950-124 (Roche). The MHCII primary was of mouse origin ...
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bioRxiv - Cancer Biology 2024Quote: ... Proliferation rate in vitro was assessed using an xCELLigence Real-Time Cell Analyzer (RTCA) system (Roche Diagnostics)49 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell extracts were obtained by washing 3 × in PBS and either lysing for RNA (TriPure reagent, Roche) or protein extraction (50 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2024Quote: ... Isolated hepatocytes were seeded at a density of 4-500,000 and 1,000,000 cells in rat tail collagen I (5 μg/cm2, Roche) pre-coated 6-well plates and T25 flasks ...
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bioRxiv - Developmental Biology 2024Quote: ... Cell lines were transfected with siRNAs using xTremeGene siRNA transfection reagent (1ul/well, Roche, #4476093001; standard protocol). After addition of siRNA transfection mix ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then transfected with the respective plasmids using X- tremeGENE™ HP DNA transfection reagent (Roche, #6365779001) for 48 h (24 h for Toca-1 and Cdc42).
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bioRxiv - Cell Biology 2020Quote: ... the cells were incubated with the anti-HA high affinity antibody (3F10) (1:500 dilution, Roche – cat. 11867423001), followed by incubation with an anti-rat secondary antibody conjugated to Alexa Fluor 488 (1:2000 dilution ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was extracted from 3 million HEK293T cells or 0.75 million primary neurons using TriPure reagent (Roche) and purified using RNeasy MinElute Cleanup Kit (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were lysed using ice-cold Farnham lab buffer supplemented with complete protease inhibitor cocktail (#4693159001, Roche, Switzerland). Chromatin was sheared with Bioruptor (Diagenode ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was isolated from asynchronous RPE-1 cells using the High Pure RNA Isolation kit (Roche, 11828665001) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then lysed using 0.4 mL lysis buffer containing 2x protease inhibitors (Complete, Mini, EDTA-free; Roche), 1x phosphatase inhibitors (PhosStop ...