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Citations for Roche :
1751 - 1800 of 2327 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Every 10 million cells were lysed by 300μL 1% SDS include freshly made Protease Inhibitor (PI) cocktail (Roche #4693132001), sonicated with Diagenode Bioruptor Pico and diluted with 2.7mL PBA (1x PBS + 0.5% BSA ...
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bioRxiv - Biochemistry 2019Quote: ... The Bacmid DNA was purified and transfected into SF9 insect cells with Xtreme gene 9 DNA transfection reagent (Roche). The first virus generation ...
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bioRxiv - Cell Biology 2019Quote: ... and packaging plasmids PEx-QV and pMD-G were co-transfected into HEK 293T cells using Fugene 6 (Roche). After 4 days ...
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bioRxiv - Cell Biology 2020Quote: Cells were lysed in hypotonic buffer (20 mM HEPES pH=8.0, 1x cOmplete EDTA-free protease inhibitor cocktail (Roche) and sonicated three times for 2 minutes ...
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bioRxiv - Cancer Biology 2021Quote: Apoptotic cells were detected using the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL assay; Roche Applied Sciences) according to the manufacturer’s recommendations.
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bioRxiv - Cell Biology 2020Quote: ... cDNA was synthesized from 400 ng (melanoblasts) or 1µg (cell lines and tissues) input RNA using Transcriptor first-strand cDNA synthesis kit (04897030001, Roche) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... The cells were lysed with 400 μL of NAD+/NADH extraction buffer containing a cocktail of protease inhibitor (Roche Diagnostic ...
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bioRxiv - Cell Biology 2021Quote: Cells were lysed in RIPA buffer or high-salt RIPA buffer supplemented with cOmplete EDTA-Free protease inhibitor (Roche) and PhosSTOP phosphatase inhibitor (Roche ...
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bioRxiv - Cancer Biology 2020Quote: ... total RNA was extracted from LOUCY cells using the High Pure RNA Isolation Kit (Roche Life Sciences, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... 12 μg of the above vector with 6 μg pCMV8.9 and pCMV-VSVG packaging vectors were transfected into the 293 packaging cell line using X-tremeGENE 9 (Roche), and the resulting viral supernatants were harvested as previously described13 ...
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bioRxiv - Cell Biology 2021Quote: ... NPCs were washed by phosphate buffer saline (PBS) and stained by in situ Cell Death Detection Kit (Roche, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Pelleted cells were resuspended in 1x PBS buffer (pH 7.4) with 5 mM BME and proteinase inhibitor cocktail (Roche). For purification of PABPC1-His ...
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bioRxiv - Cell Biology 2021Quote: ... and thiamine-dependent repression was validated by immuno blotting of whole cell extracts using anti-HA antibodies (Roche, 3F10).
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bioRxiv - Microbiology 2020Quote: ... After 24 h cells were lysed in lysis buffer (0.5 (v/v) % NP-40 in PBS supplemented with protease inhibitors (Roche) and phosphatase inhibitors (Roche) ...
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bioRxiv - Microbiology 2021Quote: Cells were transfected with mammalian construct DNA according to manufacturer instructions with X-tremeGENE 9 DNA Transfection Reagent (Roche).
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bioRxiv - Microbiology 2021Quote: ... RNA was isolated from 200 µl of virus-containing cell culture supernatants using TriPure isolation reagent (Roche Applied Science) and purified according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Protein from 2D hPSC-cardiac cell cultures was extracted using RIPA lysis buffer supplemented with protease inhibitor cocktail (Roche). Protein lysate concentration was estimated using a BCA assay (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were lysed in immunoprecipitation buffer (50mM Tris–HCl, 0.5% NP-40 PBS with protease inhibitor cocktail (Roche)) 24hr after transfection and subjected to GFP-trap (ChromoTek ...
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bioRxiv - Biophysics 2020Quote: ... a 500 mL cell pellet of Sf9 cells was thawed in 50 mL lysis buffer (25 mM HEPES pH 7.4, 1 M KCl, 10% glycerol, Roche protease inhibitor ...
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bioRxiv - Cancer Biology 2021Quote: Cell pellets and tumor-bearing mammary glands were homogenized in 1% SDS solution containing protease (cat# 11836153001, Roche AG) and phosphatase inhibitor cocktail (cat# 04906845001 ...
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bioRxiv - Cell Biology 2020Quote: ... cell nuclei were counterstained with 100 ng/ml of DAPI for 10 min at room temperature (Roche Applied Science). Slides were mounted on coverslips using Fluoromount mounting medium (DAKO) ...
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bioRxiv - Cell Biology 2021Quote: ... membranes were removed from neurons cultured in MatTek dishes at 8-10 days in vitro (DIV) by incubating cells with extraction buffer (1M sucrose, 0.15% Triton X-100 (Roche, Basel ...
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bioRxiv - Cell Biology 2021Quote: ... The pelleted cells were homogenized in 1X RIPA lysis buffer containing freshly added phosphatase and protease inhibitor cocktail (Roche). To remove cellular debris ...
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bioRxiv - Cell Biology 2022Quote: ... The labeled cells were directly harvested in 1ml of RIPA buffer including 1X EDTA-free protease inhibitor cocktail (Roche). The lysate was cleared by centrifugation at 20,000g for 15 min ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were lysed in lysis buffer (50 mM HEPES pH 7.4, 150 mM NaCl, 1% NP-40, 2x Roche EDTA-free protease inhibitors ...
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bioRxiv - Cell Biology 2022Quote: ... A single-cell solution was obtained by tissue digestion using 1mgml−1 collagenase D and 0.1mgml−1 DNase I (Roche) in HBSS (Lonza ...
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bioRxiv - Immunology 2022Quote: ... The cells were lysed in TBS/1% (v/v) IGEPAL-630 (sigma) containing 1X Complete protease cocktail inhibitors (Roche). The TCR-CD3 complex was purified from the lysate using Dynabeads M-280 streptavidin (Invitrogen) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Transfected Expi293F cell pellets were lysed in PBS + 1% Triton-X100 and Complete protease inhibitor tablets (Roche, Mannheim, Germany) for 15 minutes on ice ...
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bioRxiv - Microbiology 2022Quote: ... Cell pellets were then collected in RIPA buffer containing Complete Mini protease inhibitor cocktail (Roche Diagnostic Systems, Laval, Canada) and lysed through five cycles of freeze-thaw ...
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bioRxiv - Cancer Biology 2022Quote: ... DF1 cells were transfected with the indicated RCAS plasmid using X-tremeGENE 9 DNA transfection reagent (Roche, Catalog# 06365809001) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 x 105 DAOY wild type or 2.5 x 105 HD-MBO3 cells were lysed using RIPA buffer supplemented with protease (Complete Mini, Roche) and phosphatase inhibitors (PhosSTOP ...
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bioRxiv - Neuroscience 2020Quote: Lysates for whole cell protein analysis were obtained by resuspending cells with Tris-buffered Saline (TBS) supplemented with 0.05% NP40 with the addition of phosphatase and protease inhibitors (Roche) on ice ...
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bioRxiv - Molecular Biology 2020Quote: ... The TUNEL reaction was performed in a humid chamber for 2.5 h at 37 °C with the in Situ Cell Death Detection Kit (Roche) using 100 μL of reaction mixture per slide ...
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bioRxiv - Immunology 2021Quote: ... For experiments in which islets were analyzed using flow cytometry single cell suspensions were generated by digestion with 0.4 Wunsch Units/ml Collagenase D (Roche) and 250 µg/ml DNAse I (Roche ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were then lysed in RIPA buffer supplemented with a Complete EDTA-free protease inhibitor cocktail tablet (Roche) and Halt protease ...
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bioRxiv - Microbiology 2021Quote: ... cytotoxicity was determined by lactate dehydrogenase measurements on cell-free supernatants using the Cytotoxicity Detection Kit Plus assay (Roche) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: Protein was purified first by resuspending cell pellets in lysis buffer (50 mM Tris-HCl, 300 mM NaCl, pH 8.0, and protease inhibitor tablets (Roche)) and lysed by pulse sonication on ice for 5 minutes in three cycles of 1 s on and 1 s off ...
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bioRxiv - Microbiology 2022Quote: ... 1.5 million S2 cells were transfected with 2 μg of plasmid using Xtreme-GENE HP DNA transfection reagent (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: Apoptosis in the migrating primordium was identified using terminal transferase-mediated dUTP nick end-labeling (TUNEL) assay according to manufacturer’s instruction with minor modifications (In situ Cell Death Detection Kit, TMR Red, Roche). Embryos at 30-42 hpf were dechorionated and fixed in 4% PFA overnight at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: Real-time cell proliferation was measured using an E-plate 16 and a RTCA DP Analyser (xCelligence System, Roche), which uses gold microelectrodes fused to the bottom surface of a microtiter plate well ...
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bioRxiv - Molecular Biology 2019Quote: ... The E.coli cells were resuspended in lysis buffer (20mM HEPES, pH 7.5, 500mM NaCl, 5% glycerol supplemented with 1X PIC (Roche), 100ug/ml lysozyme and lysed by sonication and centrifuged ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were lysed with TNE (Tris) buffer containing protease (Complete Mini) and phosphatase inhibitors (PhosStop Roche, 50mM Sodium Fluoride). Protein concentrations were determined by Bradford assay and lysates stored at −80°C until use.
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bioRxiv - Neuroscience 2020Quote: TUNEL assay (90) was performed using an in situ cell death detection kit conjugated with fluorescein isothiocyanate (Roche, 11684795910). DAPI (Vectashield Antifade Mounting Medium with DAPI ...
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bioRxiv - Genomics 2019Quote: ... Cells were then harvested and genomic DNA was extracted with a High Pure PCR Template Preparation Kit (Roche 11796828001). MinION runs were performed on R9 with 400-500ng purified DNA per sample following library preparation with Ligation Sequencing Kit 1D (ONT SQK-LSK108) ...
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bioRxiv - Genetics 2020Quote: ... and one day later 2 micrograms of plasmid DNA was transfected into cells using Xtremegene HP transfection reagent (Roche) according to the manufacturer’s specifications ...
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bioRxiv - Cell Biology 2019Quote: Cells were lysed in GFP trap buffer (50 mM Tris-HCl, 0.5% NP-40 and Roche protease inhibitor cocktail) and the lysate was added to pre-equilibrated GFP trap beads (ChromoTek) ...
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bioRxiv - Microbiology 2021Quote: HEK293T cells transiently transfected with WT EphA2-eYFP were selected with 1.6 mg/ml G-418 solution (Roche; 4727878001) for 12 days to generate a stable cell line ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were harvested 48 hours post transfection and lysed in IP Lysis Buffer (Pierce) supplemented with protease inhibitors (Roche) for 45 minutes at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: Cultured cells were lysed in RIPA buffer containing protease inhibitor cocktail (1 tablet/15 mL) (Roche Diagnostics, Mannheim, Germany), sonicated and stored at −80°C until analysis ...
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bioRxiv - Microbiology 2019Quote: ... Whole cell extracts were prepared after transfection and incubated with indicated antibodies together with Protein A/G beads (Roche) overnight ...