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Citations for Roche :
1351 - 1400 of 2327 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Target cells with PBMCs in complete medium or supplemented with 5% Triton X-100 (Roche Diagnostics) were used to determine minimum and maximum release ...
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bioRxiv - Cancer Biology 2020Quote: Cells were lysed in RIPA buffer supplemented with Complete Phosphatase and Complete Protease inhibitor cocktails (Roche). After dilution in Laemmli buffer with DTT and boiled for 5 min ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were arrested at metaphase by incubating with colcemid (N-methyl-N-deacetyl-colchicine, Roche, 10295892001) for the last 14 hours before harvesting the cells ...
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bioRxiv - Cell Biology 2021Quote: Fluorescent TUNEL staining was performed using the Fluorescein In Situ Cell Death Detection Kit (11684795910, Roche), with the fluorescein detected by antibody staining using rabbit anti-FITC ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates (0.5 mg) were incubated with anti-HA antibody-coupled agarose beads (Roche, Basel, Switzerland) for 16 h at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... TUNEL staining was performed according to manufacturer’s guideline (In-Situ Cell Death Detection Kit, Fluorescein, Roche). All staining was performed on 3 hearts per group ...
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bioRxiv - Cancer Biology 2022Quote: Genomic DNA was extracted from 106 cells using High Pure PCR Template Preparation Kit (Roche, 11796828001). 150 ng of purified DNA was digested in Reaction Buffer (200 mM sodium acetate [pH 4.5] ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa cells were reverse transfected with 1:3 or 1:6 X-tremeGENE 9™ (Roche) transfection reagent according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... The cells were directly harvested in RIPA buffer including 1X EDTA free protease inhibitor cocktail (Roche) and cleared by centrifugation at 15,000g for 15 min ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293T cells were suspended in RIPA lysis buffer (T&I) containing a protease inhibitor cocktail (Roche). After centrifugation ...
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bioRxiv - Immunology 2022Quote: ... Cells of the LP were isolated from fibrous matter using collagenase IV (680 UI/ml, Roche) and DNAse I (1 mg/ml ...
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bioRxiv - Immunology 2022Quote: ... the cells were the lyzed in digest buffer with proteinase inhibitor cocktail and phosSTOP (Roche, Switzerland) on ice ...
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bioRxiv - Biochemistry 2022Quote: ... Cell membranes were solubilized in the lysis buffer plus 1% LMNG and Protease Inhibitor Cocktail (Roche) for 1 h at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were transfected with these mixtures using 2.4μL of X-tremeGENE HP DNA Transfection Reagent (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Cell pellets were resuspended in 100 or 250 μL Permeabilization Buffer containing protease (Roche, cat# 11836170001) and phosphatase (Roche ...
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bioRxiv - Neuroscience 2020Quote: ... The cell pellet was resuspended in CER I buffer supplemented with phosphatase and protease inhibitors (Roche) to isolate cytosolic fraction and lysed using CER II buffer ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed once with PBS before 350 µl of external lysis buffer (Roche, Penzberg, Germany) was added to the cells ...
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bioRxiv - Molecular Biology 2020Quote: ... cell pellets were resuspended in 750μL of ice-cold 0.05% Nonidet P-40 (Roche Diagnostics, Germany) in PBS and 200μL of the lysate was removed as “whole cell lysate” ...
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bioRxiv - Molecular Biology 2020Quote: ... the cells were lysed with the Lysis Binding Buffer from the Magnapure LC Kit 03038505001 (Roche) containing > 3 M guanidine thiocyanate (GTC) ...
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bioRxiv - Microbiology 2021Quote: ... The cDNAs were transfected into cells using the X-tremeGENE HP DNA transfection reagent (Roche, 06366546001). Then the transfected cells were infected by concentrated SARS-CoV-2 pseudotyped virus 48 h later ...
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bioRxiv - Neuroscience 2020Quote: ... The cell lysates (1 μg/μL) were enzymatically digested with 50 μg/mL pronase E (Roche) at 37°C for 1 hour followed quenching with protease inhibitors and SDS-PAGE loading buffer ...
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bioRxiv - Cell Biology 2020Quote: ... HeLa cells were transfected with Lpd-specific or scramble shRNA as control using Fugene HD (Roche) transfection reagent ...
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bioRxiv - Immunology 2021Quote: ... Th17-cell differentiation was induced using a cytokine cocktail of IL-6 (20 ng/mL; Roche), IL-1β (10 ng/mL ...
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bioRxiv - Molecular Biology 2022Quote: ... cell culture supernatants were harvested and proteins were purified from the supernatants using tandem Ni2+ (Roche) and Strep-Tactin (IBA ...
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bioRxiv - Cell Biology 2022Quote: Cells were lysed on ice in RIPA buffer supplemented with Protease Inhibitor Cocktail (Cat#. 11873580001, Roche). Lysates were cleared by centrifuging at 15,000g and 4°C for 20min (Sorvall Legend Micro 17R Centrifuge ...
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bioRxiv - Cancer Biology 2019Quote: Total RNA was extracted from cell lines using the High Pure RNA Isolation Kit (Roche, Basel). 1 μg of extracted RNA was reverse transcribed into cDNA using the Transcriptor First Strand Synthesis kit (Roche) ...
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bioRxiv - Plant Biology 2019Quote: ... according to the manufacture’s protocol of the in situ Cell Death Detection kit (Roche Diagnostics, Germany). Imaging was done with Nikon ECLIPSE 80i fluorescence microscope (Nikon ...
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bioRxiv - Cancer Biology 2019Quote: ... Total cell lysate was harvested with RIPA buffer containing protease inhibitors (protease inhibitor cocktail tablets, Roche) on the third day ...
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bioRxiv - Cancer Biology 2020Quote: Whole-cell protein lysates were harvested in lysis buffer (RIPA buffer supplemented with protease inhibitor (Roche), sodium vanadate and sodium molybdate ...
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bioRxiv - Biochemistry 2020Quote: ... coli cell pellets were resuspended in protein buffer containing cOmplete protease inhibitor cocktail (Roche, Basel, Switzerland), lysed by sonication and centrifuged for 45 min at 38,000 g to remove cell debris ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cells were washed twice with PBS and lysed with RIPA buffer containing protease inhibitor cocktail (Roche). Protein quantification was performed using the method of the Pierce™ BCA protein assay kit according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then collected and lysed in the presence of a protease inhibitor cocktail (Roche) and phosphatase inhibitors ...
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bioRxiv - Cancer Biology 2020Quote: ... Cellular extracts were prepared by incubating the cells in lysis buffer containing protease inhibitor cocktail (Roche). Anti-FLAG immunoaffinity columns were prepared using anti-FLAG M2 affinity gel (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The cell proliferation was continuously monitored by using the xCELLigence RTCA Instrument (xCELLigence RTCA, Roche, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Single cell suspensions were then prepared by incubating minced tissue in 1 mg/ml collagenase (Roche) and 50 U/ml DNase I (Roche ...
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bioRxiv - Immunology 2020Quote: ... -C and -G in a pX330 vector was transfected into HAP1 cells using X-tremeGENE (Roche). Single cells were FACS sorted based on W6/32 negativity to obtain knockout clones for HLA-A ...
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bioRxiv - Developmental Biology 2020Quote: ... was performed with the In Situ Cell Death Detection Kit/Fluorescein (Roche, 11 684 795 910). Slides were first pretreated with a 2:1 mix of Ethanol:Acetic Acid 5min -20C ...
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bioRxiv - Molecular Biology 2021Quote: ... Apoptosis was analyzed using TUNEL assay by an In Situ Cell Death Detection kit (11684795910, Roche). As counterstain for IF and IHC stainings Hoechst or DAPI staining was used ...
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bioRxiv - Microbiology 2019Quote: ... the cells were broken using an Emulsilfex C3 (Avestin) in presence of proteases inhibitor (Complete, Roche). The lysate was used to bind to 200ul of IgG Sepharose beads (Amersham Biosciences ...
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bioRxiv - Immunology 2019Quote: ... Cellular content of the preparations was assessed by cell counting using a Casey TT counter (Roche) in combination with multi-colour flow-cytometry.
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bioRxiv - Cell Biology 2021Quote: Apoptosis in kidneys was detected using the In Situ Cell Death Detection Kit (Sigma Aldrich/Roche). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cells were lysed in RIPA Lysis Buffer (CWBIO, CW2333S) containing EDTA-free cOmplete(Roche, 4693132001) with sonication on ice ...
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bioRxiv - Bioengineering 2021Quote: HEK293T cells were lysed in RIPA lysis buffer (Applygen) supplemented with complete protease inhibitor cocktail (Roche) 48 h after transfection ...
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bioRxiv - Pathology 2021Quote: ... Cell apoptosis was examined utilizing a TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) kit (Roche). In brief ...
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bioRxiv - Biophysics 2021Quote: Cells were harvested and lysed in RIPA lysis buffer containing a protease inhibitor mix (Roche #4693159001). Lysates were centrifuged at 10,000 x g for 10 min and 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... whole cell lysate of primary myoblasts was prepared by lysing buffer containing protease inhibitor cocktail (Roche). Protein concentration was determined with the BCA Protein Assay Reagent (Pierce Biotechnology ...
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bioRxiv - Biophysics 2021Quote: ... Monoclonal cell lines were identified by immunocytochemistry using a rat anti-HA antibody (Roche Applied Science). HEK293 cells stably overexpressing rat sACt were as described (58).
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bioRxiv - Immunology 2021Quote: ... Th17 cell polarization was induced using a cytokine cocktail of IL-6 (20 ng/mL; Roche), IL-1β (10 ng/mL ...
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bioRxiv - Immunology 2021Quote: ... Cellular mRNA of cells not exposed to virus was isolated with an mRNA Capture kit (Roche) and cDNA was synthesized with a reverse-transcriptase kit (Promega) ...
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bioRxiv - Genetics 2020Quote: ... Cells were permeabilized with 0.02% digitonin in the Wash Buffer along with protease inhibitors (Roche # 04693159001) for 5 mins at RT ...