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Citations for Roche :
1451 - 1500 of 2327 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... cells were stained with DAPI (1/2000 in sterile PBS from 1 mg/ml stock, Roche). Sorted cells were re-suspended in medium to reach a 2.5 million cells/ml concentration ...
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bioRxiv - Bioengineering 2024Quote: ... cells were harvested from their matrices using liberase DL research grade (24 U; Roche, Basel, Switzerland) and stained with the following antibodies (diluted 1:500 in PBS ...
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bioRxiv - Biophysics 2024Quote: ... 5% glycerol) per 1 L of cells with one EDTA-free protease inhibitor cocktail tablet (Roche). Cells were lysed using a Misonix Sonicator 3000 (110 W for 2 min total ON-time ...
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bioRxiv - Cell Biology 2024Quote: ... D-Mel (d.mel-2) cells were transfected using X-tremeGene HP DNA transfection reagent (#06366236001, Roche). Two days after transfection ...
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bioRxiv - Cell Biology 2024Quote: RNA extraction from cultured cells has been performed using the High Pure RNA Isolation Kit (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... plasmids were transfected into HeLa cells using X-tremeGENE HP DNA transfection reagent (Roche, Laval, QC) according to manufacturer’s protocols.
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bioRxiv - Cell Biology 2023Quote: ... and pLentiCRISPRv2-PFN1sgRNA (500 ng each) into HEK293FT cells using X-tremeGENE HP transfection reagent (Roche). Lentivirus was harvested 72 hours later and subsequently used to infect JR20 cells supplemented with 4 μg/mL of Polybrene ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was purified from one million cells with the High Pure RNA Isolation kit (Roche) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Protein lysates were obtained by lysing cells in 0.1% SDS and protease inhibitor cocktail tablets (Roche #04693124001). Cell lysates were separated by SDS-PAGE (Bio-Rad 4-20% Mini-PROTEAN TGX Precast Gel ...
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bioRxiv - Molecular Biology 2020Quote: Proliferative capacity of cells was analysed using 5-bromo-2’-deoxy-uridine (BrdU) labelling (Roche, Basel, Switzerland). Cells seeded on coverslips were pulsed with 10 μM BrdU for 60 min at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Whole cell extracts were prepared by lysis in RIPA buffer supplemented with protease and phosphatase inhibitors (Roche). Protein concentration was quantified by BCA assay (Thermo Fisher ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were lysed with lysis buffer (500 μL) containing the cOmplete protease inhibitor cocktail (Roche, cat#11697498001). The biotinylated surface proteins were excluded with NeutrAvidin agarose gel (Pierce ...
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bioRxiv - Cell Biology 2020Quote: ... at pH 7.4] and seeded at a density of 50–75 × 103 cells/plate on laminin (Roche) coated plates ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were harvested on ice in Pierce IP lysis buffer and EDTA-free protease inhibitor cocktail (Roche), sonicated ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed 3x with ice cold PBS and lysed with lysis buffer with protease inhibitors (Roche). Cell lysate was centrifuged for 30 min at 15000 g and supernatant was applied to streptavidin-conjugated magnetic beads (Dynabeads® MyOne™ Streptavidin C1 ...
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bioRxiv - Cell Biology 2020Quote: Mammalian cells were lysed in Radioimmunoprecipitation assay buffer (RIPA) buffer with the addition of protease (Roche cOmplete), and phosphatase inhibitors (GB Sciences) ...
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bioRxiv - Microbiology 2020Quote: ... The DNA fragmentation assay used the Cell Death Detection ELISA kit (initially from Roche, later Millipore-Sigma). Phosphatidylserine was detected by FITC-conjugated annexin V (Sigma) ...
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bioRxiv - Immunology 2021Quote: Macrophage cell lysates were lysed in radioimmunoprecipitation assay (RIPA) buffer containing protease inhibitor cocktails and PhosSTOP (Roche). Protein concentration was measured using the BCA Protein Assay Kit (Pierce) ...
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bioRxiv - Immunology 2021Quote: The cell pellets were lysed in 8M urea/50mM Tris pH 8.1/100mM NaCl + protease inhibitors (Roche) and quantified by BCA assay (Pierce) ...
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bioRxiv - Immunology 2021Quote: ... Cells were lysed in IP Lysis Buffer (87787, Pierce™) supplemented with protease inhibitors (Complete mini, Roche), and IP was performed with antibodies against survivin (RnD AF886 ...
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bioRxiv - Neuroscience 2020Quote: ... cells were fixed with 4%PFA and 100 μl of Annexin-V-Alexa 568 labeling solution (Roche) and 50 μM Sytox Green dye (Invitrogen ...
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bioRxiv - Evolutionary Biology 2021Quote: ... by using In Situ Cell Death Detection Kit (TMR red cat no. 1684795, Roche, Indianapolis, IN, USA) according to manufacturer’s recommendation ...
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bioRxiv - Immunology 2022Quote: ... and pLenti-Luc-GFP (6 µg) into HEK293T cells using X tremeGENE HP DNA Transfection Reagent (Roche) according to the manufacturer′s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... TUNEL staining was performed according to manufacturer’s instructions (In situ cell death detection kit TMR red, Roche). For the positive control ...
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bioRxiv - Developmental Biology 2019Quote: TUNEL was performed as described in [68] using In Situ Cell Death Detection Kit provided by Roche Diagnostics ...
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bioRxiv - Microbiology 2019Quote: ... cells were resuspended in buffer B1 supplemented with complete EDTA-free protease inhibitor cocktail (Roche, Basel, Switzerland), 1 mg/ml lysozyme ...
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bioRxiv - Microbiology 2019Quote: ... a first stage consisted in lysing the cell pellets by beat beating with MagNA Lyser Instrument (Roche Diagnostics ...
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bioRxiv - Microbiology 2019Quote: ... Cells were subsequently solubilised in 1% Triton X-100 in PBS containing 1x cOmplete protease inhibitor (Roche) and incubated on ice for 20 min ...
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bioRxiv - Microbiology 2019Quote: ... Infected red blood cells were lysed on ice with chilled hypotonic buffer (1 mM HEPES/NaOH, Roche cOmplete EDTA-free protease inhibitor ...
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bioRxiv - Cancer Biology 2019Quote: ... Cytofluorimetric recordings of phosphatidylserine exposure on the cell surface (increased staining of FITC-conjugated Annexin-V; Roche) and loss of plasma membrane integrity (7-AAD staining ...
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bioRxiv - Genetics 2019Quote: Total protein was extracted from transfected HEK293 cells lysed in RIPA buffer (Pierce) containing protease inhibitors (Roche) 24 hours post transfection and size separated using SDS-PAGE ...
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bioRxiv - Systems Biology 2019Quote: ... The Th17 cultured cells were stimulated in presence of IL-6 (20ng/ml, Roche, Cat# 11138600 001); IL-1β (10ng/ml ...
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bioRxiv - Biochemistry 2021Quote: ... coli cell lysate (in Buffer C supplemented with a cocktail of protease inhibitors (cOmplete, EDTA-free (Roche)) ...
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bioRxiv - Immunology 2021Quote: Mouse lung cells were obtained by digestion of lung tissue with 0.05 mg/ml Liberase TL (Roche) and 0.5 mg/ml DNaseI (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Cells were resuspended in AYE media containing a complete mini EDTA-free protease inhibitor cocktail tablet (Roche) and adjusted to an OD600 of 20 ...
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bioRxiv - Microbiology 2021Quote: Cells were washed in PBS and lysed in NTEN buffer supplemented with phosphatase and protease inhibitors (Roche). Protein concentrations were determined using a BCA assay (Pierce) ...
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bioRxiv - Bioengineering 2021Quote: Cell membrane integrity was measured via the lactate dehydrogenase activity (LDH) release assay (Roche Cytotoxicity Detection Kit). Media from the bottom channel was continuously collected in 15 minutes intervals (both for the unstretched and stretched mimicking VILI) ...
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bioRxiv - Neuroscience 2021Quote: ... for 5 minutes and cells were washed in cold PBS supplied with protease inhibitor cocktail (11873580001, Roche). Cells were stored as dry cell pellet at -80°C until further processed ...
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bioRxiv - Neuroscience 2020Quote: ... The cell suspension was filtered through a 70 µm filter with DNAse (66 U/mL) (Roche Diagnostics). Cell pellet was resuspended in 25% density gradient and centrifuged at 521 g for 20 min at 18° C ...
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bioRxiv - Cell Biology 2021Quote: Cells were re-suspended in lysis buffer containing 2x concentration of PIC (Roche complete, EDTA-free #05056489001) and 2 mM PMSF ...
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bioRxiv - Cell Biology 2021Quote: ... 2 mM β-mercaptoethanol and supplemented with complete EDTA-free cocktail tablets (1 tablet/50ml cells; Roche), 0.01 mg/ml DNase (Sigma ...
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bioRxiv - Systems Biology 2020Quote: ... The cell pellets were then lysed by adding 10 M urea containing complete protease inhibitor cocktail (Roche) and Halt™ Phosphatase Inhibitor (Thermo) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell pellets were lysed in 250 μl IP Lysis Buffer (Pierce) supplemented with complete protease inhibitors (Roche) for 30 minutes at 4 °C ...
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bioRxiv - Microbiology 2021Quote: Culture density was measured with a haemocytometer (BSFs) or a CASY model TT cell counter (Roche Diagnostics) with a 60 µm capillary and exclusion of particles with a pseudo diameter below 2.0 µm (PCFs).
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bioRxiv - Microbiology 2020Quote: ... GISAID: EPI_ISL_413570) obtained from cell culture using the MagNA Pure LC Total Nucleic Acid Isolation Kit (Roche). We determined the slope by linear regression in GraphPad Prism and defined the required levels for PCR efficiency (E ...
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bioRxiv - Cell Biology 2020Quote: Cells were lysed and proteins were purified by Complete™ Lysis-M EDTA-free kit (Roche, 04719964001). Protein samples were run on Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: Total proteins were extracted from cells using RIPA lysis buffer with a protease inhibitor cocktail (Roche, Germany). The proteins were quantified using Thermo Scientific Pierce BCA Protein Assay ...
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bioRxiv - Cell Biology 2021Quote: ... Assay was performed according to protocol in Cell proliferation kit II (XTT) (Roche, #11 465 015 001).
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bioRxiv - Biochemistry 2022Quote: ... Cells were transfected with a total of 1 µg of DNA using Fugene 6 (Roche, Branchburg, NJ) according to the manufacturer’s instructions
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bioRxiv - Molecular Biology 2022Quote: ... the cell culture suspension was supplemented with DNase I and EDTA-free protease inhibitor cocktail tablet (Roche) and lysed by passing through an EmulsiFlex-05 cell disruptor (Avestin ...