Labshake search
Citations for Roche :
1501 - 1550 of 7633 citations for ssc mir 758 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2021Quote: ... First-strand cDNA synthesis and RT-qPCR was carried out using One step RT QPCR reagent (Roche). Resulting signal was detected on Applied Biosystems Real Time PCR System ...
-
bioRxiv - Immunology 2020Quote: ... Real-time PCR was performed in a final reaction volume of 20 µL containing 10 µL FastStart universal probe master (Roche Diagnostics), 50 ng/µL lambda DNA (New England Biolabs) ...
-
bioRxiv - Physiology 2019Quote: The expression pattern of Mr-GS in various tissues at different time points was studied using qRT-PCR in Roche LightCycler 480 (Roche, USA). All used primers were presented in Table 1 ...
-
bioRxiv - Physiology 2024Quote: ... Real-time PCR was performed by using ChamQ Universal SYBR qPCR Master Mix (Q711-02, Vazyme) on the Roche LightCycler96 (Roche, 05815916001) system ...
-
bioRxiv - Physiology 2023Quote: ... Real-time PCR was performed by using ChamQ Universal SYBR qPCR Master Mix (Q711-02, Vazyme) on the Roche LightCycler96 (Roche, 05815916001) system ...
-
bioRxiv - Molecular Biology 2020Quote: Cryosections (10μM) of ventricular tissue were fixed in 4% paraformaldehyde and stained with In-situ Cell Death Detection kit (Roche). Sections were imaged on a laser-scanning confocal microscope (LSM 510 ...
-
bioRxiv - Genetics 2020Quote: ... Staining for apoptotic cells was performed using the AP-In situ Cell Death Detection Kit (Roche Diagnostics, Penzberg, Germany) following manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2021Quote: ... NPCs were washed by phosphate buffer saline (PBS) and stained by in situ Cell Death Detection Kit (Roche, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2021Quote: ... The troponin T detection kit (Elecsys® Troponin T Gen 5 STAT) and troponin T were purchased from Roche. 1X phosphate-buffered saline (PBS ...
-
bioRxiv - Molecular Biology 2022Quote: Southern blot analysis was performed using the DIG High Prime DNA Labeling and Detection Starter Kit II (Roche 11585614910) and the DIG Wash and Block Buffer Set (Roche 11585762001) ...
-
bioRxiv - Cancer Biology 2022Quote: Pax6a digoxigenin (DIG) antisense RNA probes were generated from linearised plasmids using an RNA labelling and detection kit (Roche) (Scholpp and Brand ...
-
bioRxiv - Molecular Biology 2020Quote: ... The TUNEL reaction was performed in a humid chamber for 2.5 h at 37 °C with the in Situ Cell Death Detection Kit (Roche) using 100 μL of reaction mixture per slide ...
-
bioRxiv - Microbiology 2021Quote: ... cytotoxicity was determined by lactate dehydrogenase measurements on cell-free supernatants using the Cytotoxicity Detection Kit Plus assay (Roche) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... detection of bound conjugate was achieved by the use of an enhanced chemiluminescence assay (ECL) kit (Roche Applied Science). Images were acquired using a BIO-RAD ChemiDoc imaging system.
-
bioRxiv - Developmental Biology 2022Quote: Apoptosis in the migrating primordium was identified using terminal transferase-mediated dUTP nick end-labeling (TUNEL) assay according to manufacturer’s instruction with minor modifications (In situ Cell Death Detection Kit, TMR Red, Roche). Embryos at 30-42 hpf were dechorionated and fixed in 4% PFA overnight at 4°C ...
-
bioRxiv - Pathology 2022Quote: The supernatant from cultured NRVMs was collected to determine the content of LDH via cytotoxicity detection kit (Roche, USA), following the manufacturer’s instructions.
-
bioRxiv - Synthetic Biology 2020Quote: ... Washing and detection steps were carried out as outlined in the manufacturer’s protocol for the DIG High Prime DNA Labeling and Detection Starter Kit II (Roche).
-
bioRxiv - Neuroscience 2020Quote: TUNEL assay (90) was performed using an in situ cell death detection kit conjugated with fluorescein isothiocyanate (Roche, 11684795910). DAPI (Vectashield Antifade Mounting Medium with DAPI ...
-
bioRxiv - Cell Biology 2020Quote: ... Apoptotic cells were detected in dissected guts using the TMR red In Situ Cell Death Detection Kit (Roche; 12156792910) following standard kit staining protocol ...
-
bioRxiv - Immunology 2020Quote: ... Parasite cultures were confirmed to be free of mycoplasma and acholeplasma using an ELISA-based Mycoplasma Detection Kit (Roche) which contains polyclonal antibodies specific for M ...
-
bioRxiv - Genetics 2020Quote: ... Southern blot was performed according to the manufacturer’s protocol of DIG HIGH prime DNA labeling and detection starter kit 1 (Roche). 10 µg aliquot of genomic DNA was digested overnight with DraIII ...
-
bioRxiv - Cell Biology 2021Quote: TUNEL assay [47] was performed using an in situ cell death detection kit conjugated with fluorescein isothiocyanate (Roche, 11684795910). DAPI (Vectashield Antifade Mounting Medium with DAPI ...
-
bioRxiv - Developmental Biology 2022Quote: ... The TUNEL method was used to quantify apoptosis following the manufacturer’s instructions (In Situ Cell Death Detection kit; Roche). The percentage of TUNEL-positive cells in each kidney was quantified as described above ...
-
bioRxiv - Developmental Biology 2022Quote: ... TUNEL staining was performed following citrate buffer antigen retrieval using an in situ cell death detection kit (Roche, 11684795910). Positive control slide was treated with deoxyribonuclease to induce DNA damage (Sigma ...
-
bioRxiv - Immunology 2022Quote: ... Apoptotic cells were detected in dissected guts using the TMR red In Situ Cell Death Detection Kit (Roche; 12156792910) following standard kit staining protocol ...
-
bioRxiv - Molecular Biology 2022Quote: The psyllid midguts were diagnosed for cell-death or apoptosis using In situ cell-detection kit TMR-red (Roche) following the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2023Quote: ... Livers were then dissected out of the larvae and subjected to TUNEL assay according to manufacturer’s instructions (In Situ Cell Death Detection kit, Fluorescein; Roche). Nuclei were counterstained with Hoechst (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2023Quote: ... cells and sections were processed for BrdU immunodetection following the manufacturer’s recommendations (5-Bromo-2′-deoxy- uridine Labelling and Detection Kit I, Roche). The slides were mounted for fluorescent microscopy in ProLong® Diamond Antifade Mountant with DAPI (Life Technologies).
-
bioRxiv - Physiology 2023Quote: ... Cell death and DNA fragmentation were detected with a TUNEL In Situ Cell Death Detection Kit (Roche, Cat. 12156792910). Frozen pancreata for histology were removed and fixed as described above ...
-
bioRxiv - Cell Biology 2023Quote: ... The TUNEL assay was performed using the in situ Cell Death Detection Kit (11684817910, Roche Applied Science, Penzberg, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... The PCR DIG Probe Synthesis kit for probe synthesis and the CSPD luminescent detection kit for visualization of the bound probe were used according to the manufacturer’s instructions (Roche Molecular Biochemicals ...
-
bioRxiv - Physiology 2023Quote: ... Cell apoptosis was measured using an in situ cell death detection kit and following the manufacturer protocol (Roche, 12156792910). Images were obtained using an inverted fluorescent microscope (Leica DMi8 ...
-
bioRxiv - Molecular Biology 2023Quote: ... TUNEL staining was performed as in (46) using the TMR red in situ cell death detection kit (Roche, 12156792910).
-
bioRxiv - Neuroscience 2024Quote: ... 30 μm sections were stained according to instruction of In situ Cell Death Detection Kit (Roche, Basel, Switzerland, #11684817910). Briefly ...
-
bioRxiv - Microbiology 2021Quote: ... Genomic DNA was isolated using a commercial extraction kit (High Pure PCR template preparation kit; Roche Diagnostics). Indexed paired-end libraries were preparedwith the Nextera XT DNA library preparation kit (Illumina Inc ...
-
bioRxiv - Developmental Biology 2021Quote: Mice ear notches or embryo tail tips were genotyped by PCR using Kapa2G Robust HotStart PCR Kit (Kapa Biosystems, KK5517) and Lbx1 primers (Table S1) ...
-
bioRxiv - Microbiology 2019Quote: ... PCR amplicons were purified using the High Pure PCR Product Purification Kit of ROCHE™ (Roche Diagnostics Gmb, Mannheim, Germany). Sequencing was performed using MiSeq™ Illumina® (2 x 300 bp ...
-
bioRxiv - Bioengineering 2019Quote: ... the entire miniprepped volume was used as template in a PCR reaction using the KAPA HiFi PCR Kit (Kapa Biosystems). A ten-fold dilution of this PCR product was used as template in a barcoding PCR adding Illumina adapter sequences and dual barcodes ...
-
bioRxiv - Microbiology 2020Quote: ... DNA probes for 5S rRNA and SPA0079 were amplified with PCR using primers described in Table S2 and PCR DIG Probe Synthesis Kit (#11636090910, Roche). The reaction was performed with a total volume of 50 μl containing 0.2 μM of forward primer ...
-
bioRxiv - Developmental Biology 2020Quote: ... PCR optimisation was carried out on the full-length cDNA using the KAPA HiFi PCR kit (Kapa Biosystems, Boston USA) and 12 cycles was sufficient to generate the material required for SMRTbell library preparation ...
-
bioRxiv - Plant Biology 2021Quote: ... 50 ng of DNA was subjected to PCR amplification using the Kapa HiFi HotStart PCR kit (Kapa Biosystems, Wilmington, USA). The individual PCR reactions were performed in 20 μL final volume and contained:
-
bioRxiv - Microbiology 2021Quote: Alkali-labile digoxigenin labelled deoxyuridine triphosphate (DIG-dUTP) was incorporated in Southern blot probes by PCR using PCR DIG Probe Synthesis kit (Roche) with the following specifications (refer to sequences in Supplementary table 3) ...
-
bioRxiv - Physiology 2021Quote: ... All mice were genotyped by polymerase chain reaction (PCR) using KAPA Taq PCR Kits (Kapa Biosystems, Inc., Wilmington, MA, USA). Specific primers purchased from Integrated DNA Technologies (Coralville ...
-
bioRxiv - Neuroscience 2022Quote: ... PCR was carried out using the KAPA HiFi Hot Start Ready Mix PCR Kit (Cat# KK2602, KAPA Biosystems, Wilmington, MA). Briefly ...
-
bioRxiv - Plant Biology 2023Quote: ... Paromomycin probe was labeled with dUTP-DIG during PCR reaction (PCR DIG probe synthesis kit, Roche; see table primers list). Membrane was pre-hybridized 1h at 50°C in a DIG eazy Hyb (Roche ...
-
bioRxiv - Molecular Biology 2024Quote: ... A first PCR reaction was prepared from genomic DNA using the KAPA HiFi HotStart PCR Kit (KAPA Biosystems/Roche Sequencing) in a total reaction volume of 20 µL ...
-
bioRxiv - Molecular Biology 2024Quote: ... A first PCR reaction was prepared from genomic DNA using the KAPA HiFi HotStart PCR Kit (KAPA Biosystems/Roche Sequencing) in a total reaction volume of 20 µL ...
-
bioRxiv - Immunology 2024Quote: ... One hundred ng of genomic DNA was used for PCR amplification of the target loci using KAPA HiFi HotStart PCR kit (Roche) and the primer sets in Table S1 ...
-
bioRxiv - Cell Biology 2019Quote: ... The concentration of indexed libraries was quantified by RT–qPCR using the Universal Kapa Library Quantification Kit (KAPA Biosystems). Final libraries were normalized and sequenced on an Illumina HiSeq 2000 sequencer.
-
bioRxiv - Plant Biology 2023Quote: ... RT-qPCR was performed using a Roche LightCycler 480 system with a SYBR Fast qPCR Kit (KAPA Biosystems, KK4601). The expression levels of the target genes were normalized against that of tomato ACTIN2 ...