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Citations for Roche :
1401 - 1450 of 7633 citations for ssc mir 758 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The DNA fragmentation assay used the Cell Death Detection ELISA kit (initially from Roche, later Millipore-Sigma). Phosphatidylserine was detected by FITC-conjugated annexin V (Sigma) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... by using In Situ Cell Death Detection Kit (TMR red cat no. 1684795, Roche, Indianapolis, IN, USA) according to manufacturer’s recommendation ...
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bioRxiv - Neuroscience 2021Quote: ... TUNEL staining was performed according to manufacturer’s instructions (In situ cell death detection kit TMR red, Roche). For the positive control ...
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bioRxiv - Developmental Biology 2019Quote: TUNEL was performed as described in [68] using In Situ Cell Death Detection Kit provided by Roche Diagnostics ...
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bioRxiv - Microbiology 2019Quote: ... as well as Southern blot using DIG-High Prime DNA labeling and Detection starter kit II (Roche to verify only single insertion of the NAT cassette into each of the strains (577bp) ...
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bioRxiv - Genomics 2021Quote: Lactate dehydrogenase (LDH) release in the apical medium was measured with the Cytotoxicity Detection Kit (Roche 04744926001) as described previously (36) ...
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bioRxiv - Bioengineering 2021Quote: Cell membrane integrity was measured via the lactate dehydrogenase activity (LDH) release assay (Roche Cytotoxicity Detection Kit). Media from the bottom channel was continuously collected in 15 minutes intervals (both for the unstretched and stretched mimicking VILI) ...
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bioRxiv - Plant Biology 2021Quote: ... For post-hybridization and signal detection the procedure mentioned in the kit was followed (Roche Diagnostics, Germany).
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bioRxiv - Microbiology 2019Quote: ... were performed using the DIG High Prime DNA Labelling and Detection Starter Kit I (Roche, Basel, Switzerland). HindIII produces 8 kb ...
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bioRxiv - Developmental Biology 2020Quote: ... TUNEL stainings were performed as IF experiments with the TMRRed In situ dell death detection kit (Roche).
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bioRxiv - Cancer Biology 2020Quote: ... All the cells in culture were negative on periodically testing for mycoplasma using Mycoplasma Detection Kit (Roche). All the human and mouse cells ...
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bioRxiv - Genetics 2022Quote: ... Southern blotting was performed using DIG High Prime DNA Labeling and Detection Starter Kit II (Roche 11585614910) and DIG Wash and Block Buffer Set (Roche 11585762001 ...
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bioRxiv - Immunology 2022Quote: ... LDH release into the supernatants was quantified by colorimetric assay using the Cytotoxicity Detection kit Plus (Roche), which measures the conversion of tetrazolium salt in formazan ...
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bioRxiv - Cancer Biology 2020Quote: ... a standardized automated protocol using the OptiView DAB IHC Detection Kit (BenchMark GT automated immunostainer, Ventana, Roche) was performed ...
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Synthetic lethal targeting of TET2-mutant hematopoietic stem and progenitor cells by XPO1 inhibitorsbioRxiv - Cancer Biology 2022Quote: ... TUNEL apoptosis assay was performed on embryos with the In-Situ Cell Death Detection kit (POD: Roche) according to the manufacturer’s recommendations at 37°C for 5 hours ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... blastocytes were subject to the TUNEL reaction using the In-Situ Cell Death Detection Kit (Roche, 11684809910) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: TUNEL assay was carried out following the manufacturer’s instructions (In Situ Cell Death Detection Kit, Roche-11684795910).
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bioRxiv - Molecular Biology 2023Quote: ... the LDH present in cell-free supernatants was detected in triplicates using the Cytotoxicity Detection kit (Roche) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 200 µL of cell culture supernatant was collected and the Cytotoxicity Detection Kit (LDH) (Roche, Indianapolis, IN) was then utilized to detect LDH levels in the culture supernatant ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell-free supernatants were collected and assessed via LDH release assay with a Cytotoxicity Detection Kit (Roche). The percent LDH release was calculated according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... ADCC in no click or click mAbs conditions was evaluated using the Cytotoxicity Detection Kit (LDH, Roche) as instructed by the manufacturer.
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bioRxiv - Microbiology 2023Quote: ... according to the manufacturer and revealed using the DIG Luminescent Detection Kit and DIG Easy Hyb (Roche).
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bioRxiv - Cancer Biology 2023Quote: ... The BrdU-incorporation assay was performed using the BrdU Labeling and Detection Kit I (Roche, #11296736001, RRID:AB_2814711) according to the manufacturer’s instruction.
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bioRxiv - Microbiology 2020Quote: ... whereas for site 15 we used 0.7 g of sediment per sample and diluted the final extracts 10 times in ultrapure PCR water (Roche). DNA templates were used in quantitative PCR (qPCR ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA was diluted 1:5 in PCR-grade dH2O and 1.5 µl of the cDNA-mix was used per 20 µl real-time PCR reaction with 0.25 µM of each primer and 2 x FastStart Essential DNA Green Master Mix (Roche). For each target and reference gene ...
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bioRxiv - Cell Biology 2022Quote: ... diluted 10 times and 1 μl was used in 5-μl PCR reaction on a LightCycler 480 (Roche Diagnostics) with Luna Master Mix (New England Biolabs ...
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bioRxiv - Cancer Biology 2023Quote: ... miR-122 inhibition was carried out by transfection with AntagomiR 122 (Miravirsen, SPC-3649; Roche, formerly Santaris Pharma). For Smad4 silencing in mouse primary hepatocytes ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Post-tagmentation gap filling and PCR amplification were performed using the KAPA HiFi HotStart PCR Kit (Kapa Biosystems) with the following cycling parameters ...
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bioRxiv - Genomics 2019Quote: ... PCR optimisation was carried out on the full-length cDNA using the KAPA HiFi PCR kit (Kapa Biosystems) and 10—12 cycles was sufficient to generate the material required for SMRTbell library preparation ...
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bioRxiv - Immunology 2021Quote: ... The second semi-nested PCR was performed using a KAPA HiFi HotStart PCR Kit (7958897001, Roche, Basel, Switzerland) under the following conditions ...
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bioRxiv - Genetics 2022Quote: ... purpureum was labeled with DIG using PCR as described in the PCR-DIG Probe Synthesis Kit (Roche Diagnostics). 35S rDNA was labeled with DIG or biotin-16-dUTP (Roche ...
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bioRxiv - Genetics 2020Quote: ... and 90 ng of each used for PCR using KAPA HiFi HotStart Ready Mix PCR kit (Kapa Biosystems) in 50 ul reactions ...
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bioRxiv - Microbiology 2023Quote: ... PCR was performed according to the manufacturer’s instructions of the KAPA HiFi HotStart PCR Kits (Roche, Basel Switzerland) using barcoded primers and a two-step PCR protocol (NEXTflex™ 16S V4 Amplicon-Seq Kit ...
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bioRxiv - Cell Biology 2022Quote: DNA sequencing by NGS consisted of two PCR amplification steps with KAPA HiFi HotStart ReadyMix PCR Kit (Roche). The first step (18 cycles ...
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bioRxiv - Neuroscience 2023Quote: ... remaining tissue was lysed and gDNA amplified by means of PCR (KAPA2G Fast HotStart PCR kit, KAPA Biosystems) as described above ...
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bioRxiv - Genomics 2023Quote: ... A digoxigenin labeled probe was generated by PCR following the manufacturer’s instructions (PCR DIG Probe Synthesis Kit,Roche). The 993 bp probe (Cd709 chr1 ...
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bioRxiv - Cell Biology 2022Quote: ... then precipitated and cleaned before resuspension in hybridization buffer (65% formamide, 5X SSC, 5X Denhardts with 1% blocking reagent (Roche), 0.5 mg/mL salmon sperm DNA added fresh) ...
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bioRxiv - Genetics 2022Quote: ... Samples were blocked for 30 minutes in a solution of 1% BSA in 2x SSC with 0.1% tween at room temperature and then rhodamine-conjugated anti-digoxigenin (Roche, #11207750910) or FITC-conjugated anti-biotin (AbCam ...
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bioRxiv - Developmental Biology 2021Quote: ... 5X SSC, 100 µg/ml heparin, 100 µg/ml yeast RNA, 0.1% TritonX-100, 0.1% CHAPS, 2% Roche blocking reagent) for more than an hour at 60–65 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... Blots were washed with high stringency (0.5× SSC, 70°C) and developed with alkaline phosphatase-conjugated anti-DIG antibody (Roche, Switzerland) with CDP-Star substrate in accordance with the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... blots were hybridized with 10 pmol/ml DIG-labeled (CAG)7 (5′-gcAgCagcAgca-3′) at 70°C for 4 h in hybridization buffer (5 × SSC, 1% block solution [Roche, Switzerland] ...
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bioRxiv - Genetics 2021Quote: ... The RT-PCR reaction was carried out in a Light Cycler 480 fluorescence quantitative meter (Fritz Hoffmann-La Roche Co. Ltd., Basel, Switzerland).
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bioRxiv - Cancer Biology 2020Quote: ... EZ-ECL chemiluminescence detection reagent was then added (Geneflow) and membrane exposed to detection film (Roche).
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bioRxiv - Neuroscience 2022Quote: ... Real time PCR for tuft cell markers and antimicrobial peptide genes was performed using Fast Start Universal SYBR Green Master (Roche) and ViiA™7 Real-Time PCR System (Life Technologies) ...
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bioRxiv - Cancer Biology 2019Quote: Real-time PCR analysis of cDNA samples was performed with specific primers and probes designed by using Assay Design Center (Roche). Primers and probes used here are available upon request ...
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bioRxiv - Immunology 2023Quote: ... Real-time PCR was performed using qPCR primer pairs listed in Table S2 and LightCycler480 SYBR Green I Master Mix (Roche) in a LightCycler480 instrument ...
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bioRxiv - Immunology 2021Quote: The presence of lactate dehydrogenase (LDH) in cell culture supernatants was measured using the Cytotoxicity Detection kit (Roche), following manufacturer’s instructions ...
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bioRxiv - Pathology 2020Quote: ... for 20 min at 37 °C and then visualized by the DAB Map detection kit (Roche Diagnostics KK). As the negative control the primary antibody was omitted and was being replaced by the non-immune serum ...
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bioRxiv - Genomics 2020Quote: Southern blots were performed using the DIG High Prime DNA Labeling and Detection Starter Kit II (Roche 11585614910) and the DIG Wash and Block Buffer Set (Roche 11585762001) ...
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bioRxiv - Cancer Biology 2021Quote: The TUNEL staining was performed using an In Situ Cell Death Detection Kit (Roche Diagnostics GmbH, Mannheim, Germany). The paraffin sections were stained with TUNEL reaction mixture for 1 h at 37°C and then mounted using DAPI containing mount medium ...