Labshake search
Citations for Roche :
1051 - 1100 of 2582 citations for 8 9 5 α CHOLESTEN 24 METHYLENE 3 β OL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2024Quote: ... 5 mM magnesium chloride and protease inhibitors (Roche). A FastPrep-24 (MP Bio ...
-
bioRxiv - Microbiology 2024Quote: ... 5 µg/mL DNAse and protease inhibitors (Roche) and lysed by sonication ...
-
bioRxiv - Microbiology 2024Quote: ... 5 µg/mL DNAse and protease inhibitors (Roche). Lysate was incubated with 1% N-Dodecyl-β-D-maltoside for 1 hour at 4°C with mixing and then spun down at 20,000g for 20 minutes at 4°C to remove cell debris ...
-
bioRxiv - Neuroscience 2024Quote: ... midazolam (5 mg/kg body weight; Dormicum, Roche) and medetomidine (0.5 mg/kg body weight ...
-
bioRxiv - Cell Biology 2024Quote: ... treated with 5 μg/mL Proteinase K (Roche) and post-fixed in 4% (vol/vol ...
-
bioRxiv - Developmental Biology 2024Quote: ... + 5% SDS + 2x cOmplete protease inhibitor cocktail (Roche). After centrifugation at 14k rpm for 10 min at RT ...
-
bioRxiv - Biochemistry 2023Quote: ... 5 mM DTT and 1x Protease inhibitor (Roche)) ...
-
bioRxiv - Genetics 2023Quote: ... and 5 µL 2x KAPA HiFi ReadyMix (Roche). The following indexing primers were used (X indicates the positions of the 8 bp indices):
-
bioRxiv - Genetics 2023Quote: ... 5 µL KAPA HiFi HotStart ReadyMix (KAPA Biosystems) and 1.8 µL H2O HyPure ...
-
bioRxiv - Developmental Biology 2023Quote: ... and 5 U/mL deoxyribonuclease I (Roche 1010459001)) ...
-
bioRxiv - Neuroscience 2023Quote: ... and laminin at 5 μg/ml (Roche # 11243217001). Cells were cultured in the presence of differentiation factors for 40 days ...
-
bioRxiv - Developmental Biology 2023Quote: ... and 5 U/ml dispase II (4942078001, Roche). Hydrogels were seeded at a concentration of 500 cells per 200 μL gel (2.5 cells/μL) ...
-
bioRxiv - Plant Biology 2023Quote: ... and 5 µg/mL DAPI (Cat# 10236276001, Roche).
-
bioRxiv - Microbiology 2024Quote: ... 5 μg/mL PK (Roche, catalog number 03115852001) was added to the reactions and kept on ice for 10 min ...
-
bioRxiv - Plant Biology 2024Quote: ... 5 µL 2X SYBR green master mix (Roche) and nuclease-free water added to a final volume of 10 μL per well ...
-
bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked using 5% BSA (#3117332001, Roche) in TBS pH 8 buffer solution ...
-
bioRxiv - Neuroscience 2022Quote: N2a cells were collected for expression validation from the 12-well plate at 24 h post transfection in RIPA buffer (Cell Signalling Technologies; Catalog No. 9806) in the presence of phosphatase inhibitor (Roche; Catalog No. 04906837001) and protease inhibitor (Roche ...
-
bioRxiv - Biophysics 2021Quote: ... Pellets were suspended in 50 ml of denaturation buffer (8 M GdmCl, 50 mM Tris-HCl, pH 8.0) with one protease inhibitor tablet (Roche) per L medium ...
-
bioRxiv - Cell Biology 2020Quote: Fixed cells were permeabilized using ice-cold Hi-C lysis buffer (10 mM TRIS-HCl pH 8 [Sigma], 10 mM NaCl [Sigma], 0.2 % Nonidet P-40 substitute [Sigma], 1x Complete EDTA-free Protease inhibitor [Roche]) for 30 min at 4 °C ...
-
bioRxiv - Microbiology 2019Quote: ... beads containing bound methylated DNA were resuspended in 250 μl digestion buffer (50 mM Tris pH 8, 10 mM EDTA, 0.5% SDS) with 100 μg Proteinase K (Roche) and incubated at 65°C for 4 h ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were resuspended in TBSI (50 mM Tris, 100 mM NaCl, pH 8, plus Complete Protease Inhibitor Cocktail, Roche) and broken using 0.1 mm silica spheres (Lysing Matrix B ...
-
bioRxiv - Molecular Biology 2021Quote: ... washed in cold PBS and incubated on ice in cold swelling buffer (10 mM Tris-HCl pH 8, 10 mM NaCl, 0.2% NP-40, 1 mM AEBSF and 1x complete mini EDTA-free proteasome inhibitor, Roche) for 10 min ...
-
bioRxiv - Microbiology 2020Quote: ... washed and homogenized in lysis buffer (20 mM Tris, pH 8, 0.5 mM EDTA and cOmplete protease inhibitor cocktail tablets, EDTA-free (Roche)) using a BeadBeater (Biozym ...
-
bioRxiv - Molecular Biology 2021Quote: ... washed with cold PBS and lysed for 20 minutes at 4°C in lysis buffer (10mM Tris pH 8, 10mM NaCl, 0.2% NP-40, supplemented with cOmplete proteinase inhibitors (Roche)) prior to snap freezing in 1ml lysis buffer on dry ice ...
-
bioRxiv - Cancer Biology 2019Quote: ... Cells were then vigorously resuspended in 1ml of fresh ice-cold lysis buffer (50mM Tris pH 8, 150mM NaCl, 0.5% NP-40, 1 tablet of complete protease inhibitor (04693159001, Roche)) and transferred to 9 ml of pre-chilled lysis buffer and incubated for 20 min on ice ...
-
bioRxiv - Plant Biology 2019Quote: ... 1.2 mM EDTA, 16.7 mM Tris-Cl [pH 8], 167 mM NaCl, 1x Complete EDTA-free protease inhibitor cocktail [Roche]) and immunoprecipitated a second time using FLAG M2 antibody (Sigma-Aldrich ...
-
bioRxiv - Immunology 2021Quote: ... Pellets of cross-linked cells were resuspended in shearing buffer (10 mM Tris-HCl pH 8, 0.1% SDS, 1 mM EDTA) supplemented with Complete Protease Inhibitor Cocktail (Roche), and chromatin was sheared to an average fragment size of 200-250 bp ...
-
bioRxiv - Molecular Biology 2020Quote: ... and subjected to 8 cycles of lysis in a MagNALyser (Roche – 6000 rpm, 1 min on, 2 min off). Lysate was transferred to fresh microfuge tubes and clarified by centrifugation (13,000 rpm ...
-
bioRxiv - Molecular Biology 2020Quote: ... and resuspended in proteome lysis buffer (50 mM Tris HCl pH 8.0, 8 M urea, 75 mM NaCl, 100 mM sodium butyrate, cOmplete protease inhibitors – Roche). Cells were then mechanically lysed (MagNAlyzer Roche – 6000 rpm ...
-
bioRxiv - Biochemistry 2020Quote: ... Elution buffer (50 mM Na2HPO4, 0.3 M NaCl, 250 mM imidazole; pH 8) containing EDTA-free protease inhibitors (cOmplete, Roche) was used to elute the proteins ...
-
bioRxiv - Microbiology 2019Quote: ... cells were resuspended in 500 μL of urea lysis buffer (8 M urea in 50 mM Tris pH 8.2, 75 mM NaCl, Roche Complete EDTA-free Protease Inhibitor Cocktail tablet ...
-
bioRxiv - Cell Biology 2021Quote: ... membranes were removed from neurons cultured in MatTek dishes at 8-10 days in vitro (DIV) by incubating cells with extraction buffer (1M sucrose, 0.15% Triton X-100 (Roche, Basel ...
-
bioRxiv - Cell Biology 2021Quote: Cell pellets or mouse tissues were resuspended in ice-cold 8 M urea buffer (8 M urea, 50 mM Tris pH 7.4, 50 mM NaCl) supplemented with protease and phosphatase inhibitors (Roche). The resuspended samples were sonicated ...
-
bioRxiv - Neuroscience 2021Quote: ... resuspended in cold lysis buffer (25 mM Tris, pH 8, 0.02% w/v NaN3, Complete protease inhibitor, Roche, 04693132001) and lysed using the CF cell disruptor (Constant Systems Ltd ...
-
bioRxiv - Cancer Biology 2021Quote: ... for TWIST1 and in a CC1 buffer of pH 8 (CC1 = Tris-Borate/ EDTA, Ventana Medical Systems ROCHE Diagnostics) for TWIST2 ...
-
bioRxiv - Cell Biology 2022Quote: ... 10mM Tris-HCl pH 8, 1 mM DTT, 0.2% NP-40, 1x protease inhibitor [Roche], 1x phosphatase inhibitor [Roche]), sonicated and centrifugated for 10 minutes at 14,000 RPM at 4°C ...
-
bioRxiv - Molecular Biology 2019Quote: ... Cell pellets were incubated in 1 ml ice-cold lysis buffer (10 mM Tris-HCl pH 8, 10 mM NaCl, 0.2% NP-40, protease inhibitor cocktail (Roche) for 15 min on ice ...
-
bioRxiv - Biochemistry 2019Quote: ... washed with PBS and suspended in Ni-wash buffer (20 mM Tris/HCl pH 8, 10 mM imidazole, 200 mM NaCl, 10 mM DTT, 2 mM PMSF, 0.5x Roche Protease Inhibitor cocktail ...
-
bioRxiv - Molecular Biology 2020Quote: ... Released nuclei were washed (10 mM Tris-HCl pH 8, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 1x PIC (Roche)) for 5 minutes at 4°C ...
-
bioRxiv - Genomics 2020Quote: ... Isolated nuclei were then lysed with lysis buffer (50mM Tris-HCl, pH 8.1, 10 mM EDTA, pH 8, 1% SDS, 1X protease inhibitor cOmplete EDTA-free cocktail, Roche). Nuclei were sonicated using a Bioruptor ultrasonic cell disruptor (Diagenode ...
-
bioRxiv - Genomics 2019Quote: All 5’ fragments were amplified off the array using HSSF-ATGC and DO_15R_PU (Supplemental Table 8) with KAPA HiFi HotStart Uracil+ ReadyMix PCR Kit (Kapa Biosystems) and stopped before plateauing ...
-
bioRxiv - Molecular Biology 2020Quote: ... The digested RNA samples were then analyzed by electrophoresis (5.5% PAGE with 8 M urea) and northern blotting using the DIG Northern Starter Kit (Roche). Poly(A ...
-
bioRxiv - Genomics 2021Quote: ... a junction PCR was performed using an intron-spanning primer pair (Junction_fwd and Junction_rev) and 8 μl cDNA with the KAPA HotStart HiFi Ready Mix (KAPA Biosystems) for 15 cycles ...
-
bioRxiv - Cell Biology 2019Quote: Cells were harvested and lysed in ice-cold lysis buffer (20 mM Tris pH 8, 200 mM NaCl, 1 mM EDTA, 0.5% NP40) with protease inhibitor cocktail (Roche). Protein content of extracts was estimated using Pierce 660 nm Protein Assay Reagent (Thermo Fisher ...
-
bioRxiv - Cell Biology 2020Quote: ... lower hind limb muscles from C57BL/6 mice aged 6-8 weeks were dissected and digested with collagenase (Roche). Isolated cells were plated on matrigel-coated dishes and allowed to grow in DMEM containing 20% fetal bovine serum (FBS ...
-
bioRxiv - Immunology 2021Quote: ... Add 120 ul of fresh ChIP SDS Lysis Buffer (0.5% SDS, 50 mM Tris, pH 8, 10 mM EDTA, 1 × protease inhibitor cocktail (Roche)) per 5 million cells ...
-
bioRxiv - Immunology 2022Quote: ... were lysed in 400 μl lysis buffer in urea buffer (8 M urea, 50 mM Tris pH 8.0, 1 mM TCEP) with protease (cOmplete mini EDTA free, Roche) and with phosphatase inhibitors (PhosStop ...
-
bioRxiv - Biophysics 2022Quote: ... Frozen bacteria containing full-length or ΔUBLΔUBA UBQLN constructs were resuspended in Buffer A (50 mM Tris pH 8, 1 mM MgCl2, 1 mM PMSF, and 0.2 mg/mL DNase I and Roche Complete Mini protease inhibitor cocktail) ...
-
bioRxiv - Cell Biology 2022Quote: ... The pellet was resuspended with 20 mL lysis buffer (50 mM NaH2PO4, 0.5 M NaCl, pH 8) and 1 cOmplete Mini Protease Inhibitor Cocktail tablet (Roche) per 1 L of expression ...
-
bioRxiv - Genomics 2022Quote: ... before being re-pelleted by centrifuging for 3 minutes at 4°C at 500g and resuspended in 180µl TMS buffer (10mM Tris-HCl pH 8, 1mM MgCl2, 1% SDS, 1× Complete Protease Inhibitor Cocktail (PIC, Roche)) with 0.4µl benzonase (E1014 ...