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Citations for Roche :
851 - 900 of 2467 citations for 8 9 5 α CHOLESTEN 24 METHYLENE 3 β OL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... 10 mM NaCl, 1% SDS, 0.1 mM dithiothreitol, 20 μM PMSF, 0.5% β-mercaptoethanol, 1X proteinase inhibitor from Roche) and centrifuged for 10 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The Ni-NTA agarose was rotated for 30 min at 4°C with 10 mL of elution buffer (50 mM Na-phosphate buffer pH 8.0, 500 mM NaCl, 1.4 mM β-mercaptoethanol, protease inhibitors [1 Roche Mini EDTA-free tablet] ...
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bioRxiv - Genetics 2020Quote: ... 5 μL of glycogen (20 mg/ ml) and 5 μl of proteinase K (20 mg/ ml; Roche) were added to the samples and incubated at 37 °C for 2 hours ...
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bioRxiv - Immunology 2023Quote: ... 5 mM CaCl2 and 5 mM MgCl2 with 100× concentrated EDTA-free protease inhibitor cocktail (Roche, pallet). The suspensions were treated with Dounce and added with 25 mU ml−1 apyrase ...
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Dual MAPK and HDAC inhibition rewires the apoptotic rheostat to trigger colorectal cancer cell deathbioRxiv - Cancer Biology 2021Quote: ... 1 mM EDTA pH 8 and 1 tablet of protease inhibitor cocktail Roche cOmplete (Roche, Basel, Switzerland) and 1 tablet of phosphatase inhibitor PhosSTOP (Roche)) ...
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bioRxiv - Cell Biology 2022Quote: ... Tissues were lysed with 8 M urea in PBS supplemented with protease and phosphatase inhibitor cocktail (Roche) at room temperature ...
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bioRxiv - Plant Biology 2019Quote: ... the membrane was probed with a 1:20000 dilution of mouse anti-GFP antibody (JL-8, Roche) or mouse anti-alpha-tubulin antibody (B-511 ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 mM Tris pH 8) containing protease inhibitors (Roche mini EDTA-free, 1 tablet in 10 ml) and phosphatase inhibitors (2mM Sodium Ortho-Vanadate ...
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bioRxiv - Developmental Biology 2021Quote: ... we lysed samples in 8 μl of lysis buffer PBND containing 0.1 mg/ml Proteinase K (Roche) (Scavizzi et al. ...
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bioRxiv - Microbiology 2022Quote: ... and lentivirus vectors at a 2:8:10 μg ratio to HEK293T cells using Xtreme-Gene9 (Roche) and lentivirus-containing supernatants were collected 72hr post-transfection ...
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bioRxiv - Cell Biology 2024Quote: ... 0.5 ml ice-cold 8 % (v/v) paraformaldehyde solution in vPBS containing EDTA-free protease inhibitor (Roche) or ice-cold 8% (v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... and 0.1 mM EDTA (pH 8)) supplemented with 2 mM Na3VO4 and complete protease inhibitor mixture (Roche). Lysates were first centrifuged at 4 °C for 15 min at 20.000 g and subsequently subjected to ultracentrifugation at 4° C for 30 min at 100.000 g ...
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bioRxiv - Cell Biology 2020Quote: C2 myoblasts were transfected 18-24 h after plating at 70-80% confluence by using FuGENE 6 (Roche Diagnostics, Indianapolis, IN) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... for 2 hours at room temperature and left to incubate for 24 hours with blocking buffer containing a 1:200 dilution of anti-DIG (Roche, 11207733910) or anti-fluorescein antibody conjugated with POD (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... tissue sections were rinsed three times within 24 h with PBS plus Triton X-100 (PBST; 0.1% (vol/vol) Triton X-100 (Roche, Mannheim, Germany)) under gentle agitation at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... A total of 24 μL of the qPCR mastermix was aliquotted in each well of a 96-well lightcycler plate (Roche 96) and 1 μL of RT reaction was aliquoted in each well ...
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bioRxiv - Evolutionary Biology 2022Quote: ... A universal primer was annealed to the barcoded adapter and one of 24 indexed primers was annealed to the universal adapter followed by PCR amplification using KAPA HiFi Hotstart Readymix (Kapa BioSystems). This design ensured that only DNA sequences with both ligated adapters will be sequenced ...
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bioRxiv - Genomics 2019Quote: ... a total of 12 µg of DNA was split into 24 50 µL PCR reactions (each with 500 ng of input DNA) with KAPA2G Robust HostStart ReadyMix (Kapa Biosystems) for three cycles with a 65 °C annealing and 40 s extension ...
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bioRxiv - Molecular Biology 2021Quote: ... treated with 5.8 μl of proteinase K (24 mg/mL, P4850) for 45 min at 55°C followed by 50 μg/ml RNase A (Roche, 10109169001) for 1 h at 37°C plus 1 h at 65°C ...
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bioRxiv - Cell Biology 2021Quote: Adult fish were fasted for 24 hours then euthanized with tricaine and the glycemia was immediately measured using the Accu-Chek Aviva glucometer (Roche Diagnostics) with blood collected at the tail.
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bioRxiv - Bioengineering 2022Quote: ... Encapsulated or adherent cells were plated in a 96 well plate for 24 or 48h when WST-1 reagent (Roche, Switzerland) was added ...
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bioRxiv - Microbiology 2022Quote: Single cell suspensions from the ear were obtained by using tweezers to separate the dorsal and ventral sides of the mouse ear and placing the sheets dorsal-side down in a 24 well plate with 1 mL/well of RPMI1640 containing 0.25 mg/mL of Liberase TL (Roche, Diagnostics Corp.) and 10 μg/mL of DNase I (Sigma-Aldrich) ...
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Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeatsbioRxiv - Molecular Biology 2023Quote: ... each well of cells from 24-well plates was lysed with 120 μL of RIPA buffer with protease inhibitor (Roche, 11836153001). All protein lysates were denatured with 12% β-mercapto-ethanol in 6x SDS-loading dye ...
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bioRxiv - Microbiology 2024Quote: ... The qPCR experiments were performed using specific oligonucleotide primers previously described[24] and hot-start polymerase (SYBR Green Fast Master Mix; Roche Diagnostics). The amplification cycles were performed using a C1000 Touch Thermal cycler (Biorad) ...
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bioRxiv - Bioengineering 2024Quote: ... the cells were harvested from the matrices by digesting the collagen with liberase DL research grade (24 U; Roche, Basel, Switzerland). The cells were then fixed with 4% PFA ...
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bioRxiv - Bioengineering 2024Quote: ... the cells were harvested from the matrices by digesting the collagen with liberase DL research grade (24 U; Roche, Basel, Switzerland). The cells were then fixed with 4% PFA ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and 3 μL of Proteinase K (20 mg/mL) (Roche Diagnostics Australia Pty ...
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bioRxiv - Cancer Biology 2020Quote: ... Endogenous peroxidase activity was quenched in 3% hydrogen peroxide (Roche) in PBS for 15 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... followed by collagenase D (3 mg/mL, COLLD-RO, Roche) two times at 37 degrees ...
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bioRxiv - Genomics 2022Quote: ... and 3 μL of Proteinase K (20 mg/mL) (Roche Diagnostics Australia Pty ...
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bioRxiv - Genetics 2019Quote: ... and 3 μL of Proteinase K (20 mg/ mL) (Roche Diagnostics Australia Pty ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and 3 μL of Proteinase K (20 mg/mL) (Roche Diagnostics Australia Pty ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and 3 mL of proteinase K (20 mg/mL) (Roche Diagnostics Australia Pty ...
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bioRxiv - Developmental Biology 2023Quote: Dissected embryos were incubated with a liberase-blendzyme 3 (Roche) solution for 90min at 33 °C ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 3% bovine serum albumin (Roche Diagnostics, Meylan, France) and 1.3 mg/ml collagenase A (Merck ...
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bioRxiv - Cell Biology 2021Quote: ... 500 mM NaCl, 4 mM β-mercaptoethanol, 10 mM ATP, 2.5 mM MgCl2, cOmplete EDTA-free protease inhibitor cocktail-Roche). The beads were eluted with NTA elution buffer (1x PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Mitotic chromosomes were resuspended in 40ml MNase buffer (20mM HEPES, pH7.7; 20mM KCl; 5mM β-mercaptoethanol; 1xProtease Inhibitors cocktail (EDTA free, Roche), 20mM β-glycerophosphate,1mM Sodium orthovanadate ...
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bioRxiv - Molecular Biology 2019Quote: ... The cell pellet was resuspended in cold extraction buffer (10 mM Tris pH 7.5, 2 mM NaV, 50 mM NaF, 50 mM β-glycerophosphate, PhosSTOP Phosphatase inhibitor (Roche), cOmplete EDTA-free Protease Inhibitor Cocktail (Roche) ...
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bioRxiv - Cell Biology 2022Quote: ... 25mM Tris-HCl pH=7.5, 1% Triton-X, 10mM Na3VO4, 40mM β-glycerophosphate, 10mM NaF and protease cocktail inhibitor from Roche). 1mg of total protein lysate was incubated with 1µg/mL of purified GST or GST-SidI overnight at 4°C in a rotary mixer ...
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bioRxiv - Microbiology 2021Quote: ... Pellets were thawed on ice and suspended in 2 mL of lysis buffer A (50 mM NaPO4, pH 7.3, 300 mM NaCl, 2 mM β-mercaptoethanol, 20% glycerol and Roche cOmplete protease inhibitor (1 tablet per 10 mL)) ...
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bioRxiv - Cell Biology 2023Quote: ... A 145cm2 culture dish at 80% confluency was lysed in 600ul lysis buffer buffer (150mM NaCl, 25mM Tris pH 8.0, 20mM dodecylmaltoside, 5mM β-mercaptoethanol with cOmplete mini protease inhibitor cocktail (Roche) and PhosSTOP (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... The cell pellet containing expressed untagged RAD15 was thawed in two volumes of lysis buffer (3M NaCl, 100mM Tris pH7,5, 10% Glycerol, 4mM EDTA, 20 mM β-mercaptoethanol, protease inhibitors (Roche)) and sonicated on ice (10 pulses of 10 seconds) ...
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bioRxiv - Molecular Biology 2019Quote: ... 25 mM Tris-HCl pH 7.4, 5 mM EDTA, 5 mM MgCl2, 1% NP-40, 0.5 mM DTT, Roche mini-tablet protease inhibitor ...
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bioRxiv - Immunology 2020Quote: Isolated B cells from Gal9KO mice were lysed in NP40 lysis buffer (50 mM HEPES pH 8.0, 150 mM NaCl, 5 mM dithiothreitol, 5 mM EDTA, 0.1% Nonidet-P40, Roche cOmplete mini EDTA-free protease inhibitor ...
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bioRxiv - Cell Biology 2021Quote: ... Protein extracts were clarified by centrifugation and incubated with α-Pk clone SV5-Pk1 (AbSerotec) orα-HA clone 12CA5 (Roche) antibodies for 1 hour ...
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bioRxiv - Plant Biology 2021Quote: ... 100 µl starch degradation mix (16.8 units ml-1 amyloglucosidase [#10102857001; Roche, Mannheim, Germany] and 12 units ml-1 α-amylase [#10102814001; Roche, Mannheim ...
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bioRxiv - Plant Biology 2023Quote: ... the AIRs were treated with 1 unit per AIR mg of type I α-amylase (Roche 10102814001, Roche GmbH, Germany). The de-starched AIRs ...
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bioRxiv - Plant Biology 2023Quote: ... the AIRs were treated with 1 unit per AIR mg of type I α-amylase (Roche 10102814001, Roche GmbH, Germany). The de-starched AIRs ...
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bioRxiv - Genetics 2023Quote: ... 25 ng of probe DNA was radioactively labelled with α– dCTP 32P using High Prime (Roche, 11 585 592 001), then hybridised to the membrane overnight at 65 °C in Church solution containing 10 μg/ml sonicated Herring Sperm DNA and 10 μg/ml tRNA ...
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bioRxiv - Developmental Biology 2021Quote: ... 5 mM NaF and protease inhibitors (Roche)] ...