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Citations for Roche :
1201 - 1250 of 2467 citations for 8 9 5 α CHOLESTEN 24 METHYLENE 3 β OL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... biotin-16-dUTP or tetramethyl-rhodamine-5-dUTP (Roche) using BioPrime Array CGH and purified using BioPrime Purification Module (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... DRG tissues were incubated in 5% Collagenase A (Roche) at 37°C for 70mins and in 0.25% Trypsin (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... 5 mM Trolox) and protease inhibitors cocktail (PIC, Roche) in radioimmunoprecipitation assay (RIPA ...
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bioRxiv - Neuroscience 2023Quote: ... and 5 units/mL of DNase I (Roche Diagnostics) (digestion media ...
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bioRxiv - Genomics 2023Quote: ... protease inhibitors (1 tablet per 5 mL, Roche 4693159001)) was added to the embryo pellet ...
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bioRxiv - Neuroscience 2023Quote: ... injectable diazepam (Valium® 5 mg/mL from Roche) was diluted to 0.2 mg/mL with saline prior to administration ...
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bioRxiv - Microbiology 2023Quote: ... 5 mM 2-mercaptoethanol) with protease inhibitor cocktail (Roche) and 2 mM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Immunology 2023Quote: ... Following antibodies were diluted in 1-5% BSA (Roche): anti-cGAS (Cell Signaling ...
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bioRxiv - Microbiology 2023Quote: ... Samples were digested with 5 μg trypsin (Roche Diagnostics) overnight at 37 °C and peptides were purified with a Sep-Pak 1-cc C18 cartridge (Waters) ...
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bioRxiv - Molecular Biology 2023Quote: ... 5% glycerol with protease and phosphatase cocktail inhibitor (Roche)) was added ...
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bioRxiv - Plant Biology 2023Quote: ... 5% glycerol and EDTA-free protease inhibitor cocktail (Roche) and lysed by sonication ...
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bioRxiv - Plant Biology 2023Quote: ... 5 μl of SYBR Green Select Master Mix (Roche), and ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 5 mg/mL liberase DL (Roche, 05401160001) and 2.5 mg/mL DNase (Roche 10104159001 ...
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bioRxiv - Plant Biology 2024Quote: ... 5 mM ATP) plus the Protease inhibitor cocktail (Roche), 1 mM PMSF ...
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bioRxiv - Neuroscience 2024Quote: ... and 5 ul LightCycler SYBR Green Mastermix 2X (Roche). LightCycler 96-well plates were run on a LightCycler 96 machine (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 5 mM type 1 collagen (Roche Diagnostics) to allow spheroid formation and seeded onto the ULA plates at a concentration of 5 × 103 cells per well ...
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bioRxiv - Microbiology 2024Quote: ... 5 μl of KAPA HiFi 2X master mix (Roche) and 3 μl of diluted overnight culture grown in 96-well plates ...
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bioRxiv - Immunology 2024Quote: ... 5% glycerol) supplemented with protease inhibitors (Complete mini, Roche), and immunoprecipitation was performed with 2μg of anti-survivin antibodies (RnD AF886 ...
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bioRxiv - Cancer Biology 2019Quote: ... Pellets were resuspended in 5 mL of membrane isolation buffer (5 mM TrisHCl pH 7.6, 5 mM MgCl2, 1 mM EGTA, protease inhibitor cocktail cOmplete [Roche Applied Science, Penzberg, Germany]) with a glass dounce tissue grinder (Wheaton ...
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bioRxiv - Molecular Biology 2019Quote: ... washed once with ice cold Phy Buffer (150 mM Potassium Acetate, 5 mM Magnesium Acetate, 20 mM HEPES-KOH pH 7.4, 5 mM DTT, protease inhibitor cocktail [Roche], 10 µg/mL cycloheximide). Cell pellets were then re-suspended in 1 mL Phy Buffer ...
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Tryptophan Metabolites And Their Predicted Microbial Sources In Fecal Samples Of Healthy IndividualsbioRxiv - Microbiology 2024Quote: ... Libraries were amplified for 13 PCR cycles in 50 μl reactions containing 150 pmol of P1.1 (5’-AATGATACGGCGACCACCGAGA) and P3 (5’-CAAGCAGAAGACGGCATACGAGA) primer and Kapa HiFi HotStart Library Amplification kit (Cat# kk2612, Roche Sequencing and Life Science). Library quantification and size estimation were performed using a Fragment Analyzer (Agilent Technologies ...
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bioRxiv - Developmental Biology 2021Quote: ... 3 % SDS with protease inhibitors (cOmplete Mini EDTA-free Protease Inhibitor Cocktail, Roche) at room temperature for 1 hour in a total volume of 3 mL ...
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bioRxiv - Microbiology 2021Quote: ... 3 mM DTT and 1 mM PMSF) supplemented with protease inhibitor cocktail (Roche). Zirconium beads equivalent to 100 µL volume was added in microcentrifuge tubes and resuspended cells were lysed by 6 rounds of bead beating on a bullet blender ...
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bioRxiv - Cell Biology 2022Quote: ... collected in a 1.5 ml tube and blocked overnight in 3% BSA (Roche) before incubating in primary antibody (3% BSA in PBT ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was extracted from 3 million HEK293T?cells using TriPure reagent (Roche) and purified using RNeasy MinElute Cleanup Kit (Promega ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 mM MgCl2 with with 2X cOmplete Protease Inhibitor Cocktail EDTA-free (Roche)) for 8 minutes ...
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bioRxiv - Biophysics 2019Quote: ... Following re-suspension in 3 ml of medium containing protease inhibitor (Roche Diagnostics), the cells were cracked open using a ball bearing homogenizer with a 0.2507-inch bore and 0.2496-inch diameter ball ...
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bioRxiv - Immunology 2019Quote: ... Single-cell suspensions of lung cells were prepared by Liberase Blendzyme 3 (Roche) digestion of perfused lungs as previously described48 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were eluted by digesting the RNA with 3 µg RNase A (Roche) and 30U RNase T1 (Roche ...
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bioRxiv - Biochemistry 2022Quote: ... and 0.2% bromophenol blue) and treated with 3 mg/ml Proteinase K (Roche) for 1 h at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Each sample was subject to 3 rounds of homogenisation using a MagnaLyser (Roche) at 6,000 rpm for 15 seconds ...
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bioRxiv - Molecular Biology 2022Quote: ... Poly-guanine was added at the cDNAs 3’ end using Terminal Transferase (Roche) and dGTPs ...
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bioRxiv - Biophysics 2022Quote: GGCAGCGCTACCATAACGGA-3’) (11) by RT-qPCR was performed using LightCycler 480 II (Roche). GAPDH mRNAs were used as a loading control (14) ...
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bioRxiv - Developmental Biology 2023Quote: ... After a brief enzymatic treatment with a cocktail of Liberase Blendzyme 3 (Roche), trypsin B (BI) ...
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bioRxiv - Molecular Biology 2023Quote: ... 3×10−4 M MTG and 300 μg/ml human transferrin (Roche, 10652202001)) in a triple vent petri 10 cm dishes (Thermo fisher ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: To 2 mL of each urine sample adjusted to pH 7 by addition of 1.5 mL of phosphate buffer, 50 μL of internal standard solution (methyltestosterone, 200 ng/mL) and 50 μL of β-glucuronidase from Escherichia coli (Roche Diagnostics, Mannheim, Germany) were added ...
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bioRxiv - Biochemistry 2022Quote: ... The pellet was resuspended in 50 mL lysis buffer (20 mM Tris pH7.5, 200 mM NaCl, 2 mM β-mercaptoethanol, 10% glycerol, protease inhibitor cocktail (Roche, as directed by manufacturer), 20 mM imidazole) ...
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bioRxiv - Cell Biology 2020Quote: ... cells were collected via centrifugation and resuspended in lysis buffer (40mM Tris pH 8, 300mM NaCl, 10% glycerol, 10mM imidazole, 1mM DTT, 1mM PMSF and 1x Roche PIC). Cells were lysed by sonication and after clearing the lysate ...
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bioRxiv - Molecular Biology 2021Quote: ... resuspended in lysis buffer (100 mM Tris pH 8, 300 mM NaCl, 5ml Mg2Cl, 1mM ß-mercaptoethanol and protease inhibitor tablets (complete tablets, Roche) and lysed by sonication ...
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bioRxiv - Cell Biology 2019Quote: ... Cell pellets were resuspended in approximately five volumes of lysis buffer (50 mM NaH2PO4 pH 8, 1 M NaCl, 10 mM Imidazole, 2 mM PMSF and protease inhibitor cocktail (Roche #4693132001). Cells were lysed by sonication on ice for 12 x 30 seconds at 30 W with one minute on ice in between ...
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bioRxiv - Evolutionary Biology 2022Quote: ... were ligated to the mate pair fragments and 8 cycles of PCR was used to enrich for the final library (KAPA Biosystems). The prepared libraries were quantified using KAPA Biosystems’ next-generation sequencing library qPCR kit and run on a Roche LightCycler 480 real-time PCR instrument ...
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bioRxiv - Neuroscience 2019Quote: ... DRGs were dissected out from 8-week old adult mice and incubated with collagenase A (1 mg/ml; Roche, Basel, Switzerland) for 90 minutes and then with 1X TrypLE (Life Technologies ...
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bioRxiv - Immunology 2019Quote: ... Cells were lysed (10mM Tris-HCl [pH 8], 0.25% Triton X-100, 10mM EDTA, 100mM NaCl, Roche 1X Complete Protease Inhibitor) and nuclei were collected and re-suspended in 300μl SDS lysis buffer (1% SDS ...
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bioRxiv - Genomics 2019Quote: We amplified 5 ng of the HSS library with the following primers: STARR-Seq-AG-f and STARR-Seq-AG-r (Supplemental Table 8) using KAPA HiFi 2x Readymix (Kapa Biosystems) with a 65 °C annealing temperature and 30 s extension ...
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bioRxiv - Genomics 2019Quote: All 192 bp enhancers were amplified from the array using HSSF-ATGC and HSS-R-clon (Supplemental Table 8) with KAPA HiFi HotStart Uracil+ ReadyMix PCR Kit (Kapa Biosystems) with SYBR Green (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Illumina compatible adapters (IDT, Inc) were ligated to the mate pair fragments and amplified with 8-12 cycles of PCR (KAPA Biosystems).
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bioRxiv - Neuroscience 2020Quote: Brain tissues were homogenized in Tris-buffer (50 mM Tris pH 8, 0.25 M sucrose, EDTA-free cOmplete protease inhibitor cocktail (Roche Diagnostics 05056489001)) and centrifuged at 3600 rpm and 4°C for 30 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... 25 million cells were resuspended in 1 ml lysis buffer (1% SDS, 50 mM Tris-HCl pH 8, 20 mM EDTA, 1x cOmplete (Roche, 4693132001) protease inhibitor) ...
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bioRxiv - Microbiology 2022Quote: ... cell pellet was resuspended in prechilled buffer A (20 mM Tris/HCl, pH: 8) supplemented with cOmplete™ protease inhibitor (Roche) and 5 mM EDTA ...
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bioRxiv - Physiology 2022Quote: ... 2 mM EDTA, 20 mM Tris pH 8, 150 mM NaCl, 1 mM PMSF, 1X cOmplete™ Protease Inhibitor Cocktail, Roche) was added to the fragmented chromatin ...