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Citations for Roche :
1301 - 1350 of 2467 citations for 8 9 5 α CHOLESTEN 24 METHYLENE 3 β OL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2022Quote: ... and incubated with 5 mL Liberase (0.5 mg/mL, Roche) per kidney ...
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bioRxiv - Neuroscience 2022Quote: ... and 5 mM EDTA with cOmplete mini protease inhibitors (Roche) and phosphatase inhibitor cocktails 2 and 3 (Sigma) ...
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bioRxiv - Physiology 2023Quote: ... 5 mM EDTA: pH = 7.4) supplemented with protease inhibitors (Roche) and PhosStop tablets (Roche) ...
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bioRxiv - Genomics 2023Quote: ... The KAPA Library Quantification DNA Standards #1–5 (Roche #07960387001) were used for the standard curve ...
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bioRxiv - Biochemistry 2023Quote: ... 5% glycerol supplemented with EDTA-free Complete Protease Inhibitor (Roche) and Salt-Active Nuclease (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... 5% glycerol and a protease and phosphatase cocktail inhibitor (Roche). Lysates were rotated at 4°C for 20 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... 5% glycerol and a protease and phosphatase cocktail inhibitor (Roche). Lysates were vortexed to homogenize and incubated on ice for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... 5% glycerol) with cOmplete EDTA-free Protease Inhibitor Cocktail (Roche). After lysate centrifugation at 48.384g for 45 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... 5 mg/ml NEM and protease inhibitors (Roche, Mannheim, Germany)) ...
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bioRxiv - Plant Biology 2023Quote: ... and 5 µL of Master mix SYBR Green I (Roche). Reactions were run in triplicates using a LightCycler® 480 II (Roche ...
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bioRxiv - Microbiology 2023Quote: ... with 5 nanomoles of oligo [dT] (average length 18nt) (Roche) in TE (10mM Tris-HCl pH 8 ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM 2-mercaptoethanol (BME) and protease inhibitor cocktail (Roche) using a combination of dounce homogenization and sonication ...
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bioRxiv - Developmental Biology 2024Quote: ... 5 mM MgCl2 and 1x cOmplete protease inhibitor cocktail (Roche) and lysed using a Microfluidizer ...
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bioRxiv - Biophysics 2023Quote: ... 5 mM MgCl2 and cOmplete protease inhibitor EDTA-free (Roche), pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... 5 mM MgCl2) completed with complete protease inhibitor cocktail (Roche) and disrupted with a Bioruptor Sonicator by sonication (2 min ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked in TBS containing 5% BSA (Roche, 10735086001) for 1 hour at room temperature or overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... with glass coverslip (coated with 5 μg/cm2 fibronectin (Roche) for an hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... containing 5% beta-mercaptoethanol and protease and phosphatase inhibitors (Roche Complete Mini protease inhibitor ...
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bioRxiv - Neuroscience 2023Quote: ... 5% glycerol plus a protease inhibitor cocktail (PIC) (Roche, 046693132001). One % Triton X-100 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... 500 mM NaCl, 1 mM MgCl2, 10 mM Imidazole, 0.5% IGEPAL® CA-630, 2 mM β-Mercapto-ethanol supplemented with Roche cOmplete inhibitor cocktail tablets) at a ratio of 15 ml of buffer/g of biomass ...
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bioRxiv - Neuroscience 2022Quote: ... iMN9D cells were lysed in cold immunoprecipitation (IP) buffer (50 mM Tris HCl pH 8, 150 mM NaCl, 0.1% Igepal CA-630) containing protease inhibitors (Roche Diagnostics, COEDTAF-RO). Following 30 min incubation at 4 °C on rotator ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 106 cells were lysed for 15 min on ice in 100 μL pre-chilled lysis buffer (50 mM Tris, pH 8, 150 mM NaCl, 1% Triton-X100, 1x cOmplete EDTA-free protease inhibitor cocktail [Roche, Basel, Switzerland]). Lysates were pelleted at 20,000 x g for 15 min at 4 °C ...
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bioRxiv - Biophysics 2021Quote: ... To isolate expressed aSyn the cell pellets were resuspended in lysis buffer (10mM Tris-HCl pH 8, 1mM EDTA and EDTA-free complete protease inhibitor cocktail tablets (Roche, Basel, Switzerland), 0.2 mM phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were collected via centrifugation and lysed in lysis buffer (1.5M KCl, 20 mM Tris pH 8.0, 20 mM Imidazole pH 8, 1 mM DTT, 1 tablet of Roche protease inhibitor cocktail). Sonication was performed to further lyse cells and to shear DNA ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The quenched PFA solution was then removed and the tissue was resuspended in ice-cold Hi-C Lysis Buffer (10mM pH=8 Tris-HCl, 10mM NaCl, 0.2% NP-40 and 1x Roche Complete protease inhibitor). The lysis was helped with a Dounce Homogenizer Pestle A on ice (series of 10 strokes in 10’ intervals) ...
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bioRxiv - Plant Biology 2021Quote: ... The final clean nuclear pellet was resuspended in 1 mL nuclear lysis buffer (20 mM Tris pH 8, 2 mM EDTA, 0.1% SDS, 1 mM PMSF, 1X Roche protease inhibitor cocktail) and sonicated on the Covaris E220 (150W peak power ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting nuclear pellet was resuspended in wash buffer (10 mM Tris pH 8, 0.25 M sucrose, 10 mM MgCl2, 1 mM EDTA, 1% Triton X-100, 1X Roche protease inhibitor cocktail) and nuclei were cleaned and pelleted at 12,000 x g for 10 min at 4°C ...
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bioRxiv - Biophysics 2019Quote: ... to isolate expressed aSyn the cell pellets were resuspended in lysis buffer (10mM Tris-HCl pH 8, 1mM EDTA and EDTA-free complete protease inhibitor cocktail tablets (Roche, Basel, Switzerland), 0.2 mM phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Genomics 2022Quote: ... The nuclei were then centrifuged at 500 g for 8 minutes and resuspended in Wash Buffer (20 mM HEPES pH7.5, 150 mM NaCl, 0.5 mM spermidine, Roche Complete Protease Inhibitor Cocktail). The nuclei were either used fresh or were frozen in Wash Buffer with 10% DMSO and stored at -80°C ...
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bioRxiv - Molecular Biology 2023Quote: ... were sequenced in the forward direction on 1/8 plate of a 454 Life Sciences Genome Sequencer FLX machine (Roche, Branford, CT) using the Macrogen facilities (South Korea).
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bioRxiv - Biochemistry 2023Quote: ... Protein was extracted using NETN buffer (20 mM Tris-HCl pH 8, 420 mM NaCl, 1 mM EDTA, 0.5% IGEPAL, 1 mM DTT, and Roche Protease Inhibitor Cocktail) and were resolved in 8% Bis-Tris SDS-PAGE gels ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from the mPFC tissues of CamK2A-Cre;Gpr158fl/fl mice and the controls (n = 4) at the age of 8 weeks with Tripure Isolation Reagent (Roche, Mannheim, Germany). RNA-sequencing (RNA-seq ...
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bioRxiv - Systems Biology 2023Quote: ... Then the pulverized samples were solubilized with 1000 μL extraction buffer (4% SDS, 100 mM Tris pH 8 supplemented with a cocktail of 1X protease inhibitors (Complete mini, Roche Diagnostics GmbH), 5 mM tris(2-carboxyethyl)phosphine (TCEP) ...
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bioRxiv - Neuroscience 2023Quote: ... in Tris-EDTA buffer pH 7.8 at 95°C for 64 min in standard cell condition solution one (CC1) using a Ventana Discovery ULTRA (Roche Indianapolis IN). Blocking was then performed in an inhibitor solution for 12 minutes at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM EDTA pH 8 and 10 mM NaF completed with 1 × protease inhibitor cocktail (Pierce, #A32955) and 1 × phosphatase inhibitor cocktail (Roche PhosSTOP, #490684001). The total protein concentration was determined using Bradford assay (Protein Assay Reagent ...
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bioRxiv - Molecular Biology 2024Quote: Beads were then washed twice with Low Salt Wash Buffer (150 mM NaCl, 0.1 % SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl pH 8, 1x Roche protease inhibitor mixture), twice with High Salt Wash Buffer (500 mM NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclear pellet was suspended in 1 mL of TAP buffer (100 mM NaCl, 20 mM Tris-HCl pH 8, 2.5 mM EDTA, 10 % glycerol, 1 % Triton, 1x of PhosSTOP, 1x Roche protease inhibitor mixture) and given 20 strokes with the Dounce Homogenizer ...
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bioRxiv - Molecular Biology 2024Quote: ... twice with High Salt Wash Buffer (500 mM NaCl, 0.1 % SDS, 1 % Triton X-100, 2 mM EDTA, 20 mM Tris-HCl 8, 1x Roche protease inhibitor mixture), and twice with TE wash buffer (10 mM Tris-HCl pH 8 ...
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bioRxiv - Physiology 2020Quote: ... Sigma Aldrich) with 3% bovine serum albumin (BSA, 735078, Roche Diagnostics GmbH, Mannheim, Germany), 20 µg/ml gentamicin (G1397 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and measured each at least 3 times (technical replicates) in the LightCycler96 (Roche, Germany). We detected expression using SYBRgreen marker (Roche ...
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bioRxiv - Cancer Biology 2019Quote: ... and 1-3 ng of ChIP DNA with KAPA Library Preparation Kit (KAPA Biosystems) and NimbleGen SeqCap Adaptor Kit A or B (Roche ...
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bioRxiv - Microbiology 2020Quote: ... DENV-3 RNA was amplified by qRT-PCR (LightCycler Multiplex RNA Virus Master, Roche), using primers to the conserved 3’UTR ...
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bioRxiv - Molecular Biology 2022Quote: ... the cobas p 612 pre-analytical unit (scenario 3 and 4; Roche Diagnostics International), and the cobas p 501 post-analytical unit (scenario 4a ...
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bioRxiv - Plant Biology 2021Quote: ... Probes were labelled with the DIG Oligonucleotide 3’-End Labelling Kit 2nd generation (Roche) with probe sequences listed in Table S1 ...
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bioRxiv - Neuroscience 2021Quote: ... at the ratio of 1:3 using X-tremeGENE HP DNA Transfection Reagent (Roche) following the manufacturer’s recommended protocol ...
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bioRxiv - Immunology 2022Quote: ... containing 3 mL of RPMI media with 70 μg / mL of Liberase TM (Roche) and 30 μg / mL of Dnase I (Roche) ...
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bioRxiv - Immunology 2019Quote: Mouse lungs were homogenized in HEPES buffer with Liberase Blendzyme 3 (70ug/ml; Roche) and DNaseI (30ug/ml ...
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bioRxiv - Genomics 2021Quote: Probes 1-3 were labelled with digoxigenin-11-dUTP or biotin-11-dUTP (Roche) using BioPrime® Array CGH ...
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bioRxiv - Biophysics 2020Quote: ... The oocytes were treated with collagenase A (3 mg/ml; Roche (Grenzach-Wyhlen, Germany)) for 105 min in Ca2+-free Barth’s solution containing (in mM ...
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bioRxiv - Plant Biology 2022Quote: ... and digoxigenin-labeled using a DIG oligonucleotide 3’end labeling kit (Roche Applied Science). Ten picomoles of each probe were used for each slide ...