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Citations for Roche :
951 - 1000 of 3852 citations for hsa mir 635 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... and reverse transcribed to cDNA using anchored-oligo(dT)18 primer and Transcriptor Reverse transcription (Roche). DYNLL1 was tested for splicing defects ...
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bioRxiv - Physiology 2020Quote: ... Eluted DNA was amplified with specific primers (Table S2) using SYBR Green Master (Roche, Basel, Switzerland), and the resulting signals were normalized to input activity.
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bioRxiv - Physiology 2022Quote: ... Primers were designed using Universal Probe Library Assay Design Center (Roche Applied Science, Burgess Hill, UK) or ...
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bioRxiv - Plant Biology 2022Quote: ... thaliana using the primers as indicated in Supplemental Table S9 with high-fidelity Taq enzyme (Roche) and cloned into pGGA000 or pGGC000 GreenGate entry vector (Lampropoulos et al. ...
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bioRxiv - Immunology 2023Quote: ... All primer extensions and amplifications were performed using the KAPA Long Range HotStart Ready Mix (Roche). The resulting libraries underwent purification using KAPA HyperPure beads (Roche) ...
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bioRxiv - Microbiology 2024Quote: ... 2.5 μL of 5 μM reverse primer and 10 μL of KAPA SYBR FAST (KAPA Biosystems). The qRT-PCR was done in CFX96 Real-Time System C1000 Touch Thermal Cycler (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... for 4hrs and amplified with index primers using KAPA HiFi HotStart Uracil+ Ready Mix (KAPA Biosystems). The samples after PCR amplification were then purified using Ampure XP Beads (Beckman Coulter ...
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bioRxiv - Plant Biology 2023Quote: ... RNA (1.5 μg) was reverse-transcribed using polyT primers and an Expand Reverse Transcriptase kit (Roche) according to the manufacturer‟s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The real-time qPCR primers were designed using the universal probe library assay design center (Roche) and UCSC Genome Bioinformatics (table 3) ...
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bioRxiv - Molecular Biology 2023Quote: ... Duplicates of cDNA samples and specific primers were mixed with Lightcycler SYBR Green I Master (Roche). Reactions were carried out on a Lightcycler 480 Instrument II (Roche).
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bioRxiv - Systems Biology 2023Quote: ... The concentration of individual samples was confirmed using the KAPA Library Quantification Standards with Primer (Roche Sequencing Solutions Inc ...
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bioRxiv - Plant Biology 2023Quote: ... qPCR was performed with specific primers (Table S2) and LightCycler 480 SYBR Green I Mastermix (Roche) using the LightCycler 480 II real-time PCR device (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... Duplicates of cDNA samples and specific primers were mixed with Lightcycler SYBR Green I Master (Roche). Reactions were carried out on a Lightcycler 480 Instrument II (Roche ...
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bioRxiv - Genomics 2021Quote: ... Final libraries were PCR-amplified during 5 cycles with Kapa HIFI PCR kit (Roche, Basel, Switzerland) before standard library quality control with standard sensitivity NGS Fragment kit (Agilent ...
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bioRxiv - Neuroscience 2021Quote: ... PCR was performed with Power SYBR Green PCR Master Mix (Thermofischer) on a Lightcycler 96 (Roche) with the following primers ...
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bioRxiv - Systems Biology 2020Quote: ... One microliter of this suspension was used for colony PCR (KAPA2G Robust HotStart PCR Kit, Roche). Samples with a small amplicon size were considered positive hits ...
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bioRxiv - Systems Biology 2020Quote: ... One microliter of this suspension was used for colony PCR (KAPA2G Robust HotStart PCR Kit, Roche), whereby a ∼500-bp amplicon was amplified by a primer pair in which the 3’ end of one primer was complementary to the mutant allele ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR amplification was performed by adding 15µL PCR mix containing 0.5U KAPA HiFi HotStart (Kapa Biosystems), 1x KAPA Buffer (Kapa Biosystems) ...
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bioRxiv - Neuroscience 2020Quote: ... The resulting Hi-C library was amplified by PCR (KAPA Biosystems HiFi HotStart PCR kit, KK2502), and sequenced by Illumina 50 bp paired-end sequencing.
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bioRxiv - Microbiology 2020Quote: ... PCR was performed using the Expand High Fidelity PCR System dNTPack (Roche Diagnostics GmbH, Mannheim, Germany), with 4.5 mM MgCl2 and 3.5 U of enzyme mix used for each reaction ...
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bioRxiv - Molecular Biology 2021Quote: ... One-step quantitative real-time PCR was performed on a Lightcycler 480 II PCR system (Roche) by using SYBR Green qPCR SuperMix (Bio-rad) ...
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bioRxiv - Molecular Biology 2021Quote: ... Real-time quantitative PCR (qRT-PCR) reactions were performed using a Roche LightCycler (Roche, Grenzacherstrasse, Switzerland) and Roche SybrGreen Master Mix reagent (SybrGreen ...
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bioRxiv - Genomics 2020Quote: ... Both PCR steps were run on a real-time PCR instrument (LightCycler 480 II from Roche). DNA samples from Alu carriers (and negative controls ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Standard PCR-free libraries were prepared using KAPA Hyper PCR-free library reagents (KK8505, KAPA Biosystems). Total genomic DNA was sheared into fragments of approximately 200-600 base pairs (bp ...
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bioRxiv - Synthetic Biology 2020Quote: ... All PCRs in this section were carried out using Kapa HiFi HotStart PCR Mix (Kapa Biosystems) in 20 μl reactions ...
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bioRxiv - Synthetic Biology 2020Quote: ... All PCRs in this section were carried out using Kapa HiFi HotStart PCR Mix (Kapa Biosystems), 20 ng template and appropriate primers in 20 μl reactions ...
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bioRxiv - Synthetic Biology 2020Quote: ... All PCRs in this section were carried out using Kapa HiFi HotStart PCR Mix (Kapa Biosystems) in 20 μl reactions ...
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bioRxiv - Genetics 2020Quote: ... PCR amplification of targeted sequence were performed with KAPA HiFi HotStart ReadyMix PCR kit (Roche, Germany) and the following program ...
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bioRxiv - Developmental Biology 2020Quote: ... Quantitative PCR was performed using KAPA SYBR Green fast Master PCR Kit (Roche SFUKB-KK (04707516001). Generation of specific PCR products was confirmed by melting-curve analysis ...
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bioRxiv - Biochemistry 2021Quote: ... All PCR were performed using the Expand High Fidelity PCR System (Roche Diagnostics GmbH, Mannheim, Germany) and primers were designed using the online tool at www.leishgedit.net ...
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bioRxiv - Cell Biology 2021Quote: ... SYBR-green based quantitative real-time PCR (qRT–PCR) was performed on a LightCycler 480 (Roche). Ct values were normalized to the housekeeping gene TBP for human samples and to Actb or Ccny for the mouse samples ...
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bioRxiv - Genomics 2021Quote: ... The resulting libraries were amplified with 4 PCR cycles using KAPA HiFi Uracil+ PCR enzyme (Roche). Libraries were quality controlled on a TapeStation 2200 HSD1000.
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bioRxiv - Cell Biology 2019Quote: ... qRT-PCR was performed using the light Cycler 480 II real-time PCR machine from Roche and SYBR green detection.
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bioRxiv - Genomics 2021Quote: ... and whole transcriptome amplification (WTA) by PCR was performed using KAPA Hifi PCR Mastermix (Kapa Biosystems). WTA product was purified using Agencourt Ampure beads (Beckman Coulter ...
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bioRxiv - Microbiology 2021Quote: ... The PCR was conducted using a Kapa 2G Robust PCR kit (Kapa Biosystems, Woburn, MA, USA) in 25 µ L reactions containing ...
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bioRxiv - Developmental Biology 2021Quote: ... Quantitative real-time PCR (qRT-PCR) was performed using a LightCycler® 96 System (Roche Diagnostics) with the Thunderbird SYBR Green PCR Mix (Toyobo) ...
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bioRxiv - Molecular Biology 2022Quote: ... qRT-PCR was performed with a LightCycler 480 Real-Time PCR system (Roche Diagnostics, Mannheim, Germany) using the specific primers and LightCycler-DNA Master SYBR Green I (Roche Diagnostics ...
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bioRxiv - Plant Biology 2024Quote: ... Polymerase chain reactions (PCRs) were done employing the KAPA HiFi HotStart ReadyMix PCR Kit (#KK2601; Roche Sequencing ...
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bioRxiv - Plant Biology 2024Quote: ... q-PCR reactions were performed using FastStart Universal SYBR® Green Master (ROX) PCR mix (Roche) in a StepOne machine (Applied Biosystems) ...
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bioRxiv - Genetics 2024Quote: ... and PCR amplified using the KAPA HiFi Uracil PCR Kit (Catalogue Number: ROC-07959052001, Kapa Biosystems). The final libraries were assessed with the Agilent 2200 Tapestation System using D1000 Kit (Catalogue Number:5067-5582) ...
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bioRxiv - Cancer Biology 2023Quote: ... Quantitative real-time PCR (qRT-PCR) was performed with FastStart Essential DNA Green Master (06402712001, Roche) with 25 ng of cDNA per reaction (also ...
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bioRxiv - Neuroscience 2023Quote: Real-time PCR analysis was performed with use of LightCycler 96 real-time PCR system (Roche). The PCR parameters were following ...
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bioRxiv - Biochemistry 2023Quote: ... All PCRs were performed using the Expand High Fidelity PCR System (Roche Diagnostics GmbH, Mannheim, Germany) and primers were designed using the online tool at www.leishgedit.net ...
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bioRxiv - Genomics 2023Quote: ... PCR amplifications were performed with Expand Long Template Enzyme mix (Expand Long Template PCR system, Roche). Oligos are available in Table S4.
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bioRxiv - Microbiology 2023Quote: ... PCR was performed using the Expand High Fidelity PCR System dNTPack (Roche Diagnostics GmbH, Mannheim, Germany).
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bioRxiv - Cell Biology 2023Quote: ... Real-time PCR analysis was performed using a LightCycler® 96 Real-Time PCR System (Roche). Primers were designed for each gene with Primer3-0.4.0 software (http://bioinfo.ut.ee/primer3-0.4.0/) ...
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bioRxiv - Plant Biology 2023Quote: ... Real-time PCR analysis was performed using a LightCycler® 96 Real-Time PCR System (Roche). RT-qPCR reaction was prepared in triplicates by mixing 2.5 μl of a 25-fold diluted cDNA ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR from genomic DNA was performed using KAPA2G Fast Hotstart Ready Mix PCR Kit (Kapa Biosystems). PCR products were cloned in pGEM-T Easy Vector (Promega) ...
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bioRxiv - Synthetic Biology 2021Quote: ... KAPA2G Robust PCR kits (Roche KK5005) were used to perform colony PCR and multiplex PCR for a screening of Yeast Artificial Chromosomes (YACs) ...
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bioRxiv - Developmental Biology 2019Quote: ... dissolved in PCR grade water (Roche) at 3µg/µl and stored in aliquots at −80°C ...