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Citations for Roche :
851 - 900 of 3664 citations for hsa mir 635 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The PCR products were purified using the High Pure PCR product Purification Kit (Roche, Denmark). Subsequently the purified PCR products were sent for sequencing at LGC Genomics (Berlin ...
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bioRxiv - Zoology 2021Quote: ... Fungal ITS1 fragments were amplified by PCR using the FastStart High Fidelity PCR System (Roche) for environment matrixes and the Hot Start High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Immunology 2021Quote: ... Quantitative real-time PCR (qRT-PCR) was performed on LightCycler® 480 (Roche, Basel, Schweiz) using AbsQuant 2nd Derivative Max for obtaining the Ct value ...
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bioRxiv - Synthetic Biology 2019Quote: ... PCR reactions were carried out with the Expand High Fidelity PCR System (Roche Applied Science) or Q5 High-Fidelity DNA Polymerase (NEB) ...
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bioRxiv - Plant Biology 2021Quote: ... qRT-PCR was performed with the LightCycler 480 Real-Time SYBR Green PCR System (Roche), used primers are listed in (Supplemental Dataset 5) ...
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bioRxiv - Plant Biology 2020Quote: ... qRT-PCR experiments were performed in a Light-Cycler480 Real-Time SYBRgreen PCR system (Roche). 500 ng of RNA was reverse-transcribed with the qScript® cDNA Supermix kit (QuantaBio ...
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bioRxiv - Microbiology 2020Quote: ... joining the individual fragments by overlap PCR using the Expand Long Template PCR System (Roche)104 and adding the PCR product to B ...
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bioRxiv - Microbiology 2019Quote: ... All PCR reactions were carried out using the high fidelity KAPA hifi PCR kit (Roche). Plasmids were verified by Sanger DNA sequencing prior to further use.
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bioRxiv - Evolutionary Biology 2022Quote: ... Standard PCR- free libraries were prepared using KAPA Hyper PCR-free library reagents (KAPA Biosystems). Total genomic DNA was sheared into fragments of approximately 200-600 bp and purified using AMPure XP beads ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative real-time PCR (qRT-PCR) was performed using FastStart Universal SYBR Green Master (Roche) and the StepOnePlus thermocycler (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product was purified using the High Pure PCR Product Purification Kit (Roche, Germany) and subjected to electrophoresis in 1% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... The PCR amplicon was purified using the High Pure PCR Product Purification Kit (Roche, Germany) to remove the contaminants and the amplicon was sequenced with forward and reverse primers using Big Dye Terminator v3.1 Cycle Sequencing kit on Applied Biosystems 3730 x l Genetic Analyzer ...
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bioRxiv - Microbiology 2021Quote: ... The PCR was undertaken using a Kapa 2G Robust PCR kit (Kapa Biosystems, Woburn, MA) in 25 µL reactions containing ...
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bioRxiv - Genetics 2022Quote: ... PCR was done using KAPA HiFi HotStart ReadyMix PCR Kit (Kapa Biosystems, Wilmington, Massachusetts, US). A microcapillary nucleic acid fragment analysis for quality control of the cDNA library was conducted using a Bioanalyzer ...
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bioRxiv - Pathology 2020Quote: ... Quantitative PCR measurement was performed using the Cobas z 480 PCR system (Roche, Bâle, Switzerland). Data were analyzed with the LightCycler 480 SW 1.5 software (Roche ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR amplification was performed using KAPA HiFi HotStart Ready Mix PCR kit (Roche KK2602/07958935001); 2 μL forward and reverse primer pairs (10 μM each) ...
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bioRxiv - Microbiology 2021Quote: ... PCRs were routinely performed with the High-Fidelity Template PCR system (Roche Diagnostics, Barcelona, Spain) using an MJ Mini personal thermal cycler (Bio Rad ...
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bioRxiv - Immunology 2021Quote: ... Quantitative real-time PCR (qRT-PCR) was performed on a LightCycler® 480 (Roche Diagnostics). Assays were made in triplicates and results normalized according to the expression levels of TBP ...
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bioRxiv - Genetics 2020Quote: ... and 0.5 U KAPA2G Fast PCR enzyme (KAPA2G Fast PCR kit; KAPA Biosystems, Wilmington, USA). Amplification was performed on the Takara PCR Thermal Cycler (Takara ...
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bioRxiv - Plant Biology 2022Quote: ... qRT-PCR was performed with the LightCycler 480 Real-Time SYBR Green PCR System (Roche), used primers are listed in Table S2 ...
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bioRxiv - Physiology 2022Quote: ... Quantitative PCR of cDNAs was performed using the Quantitect SYBR green PCR Master mix (Roche) with 10 ng of cDNA (by NanoDrop ...
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bioRxiv - Molecular Biology 2022Quote: ... qRT-PCR was performed on a Light-Cycler 480 real-time PCR machine (Roche, Switzerland) by using PrimeScript™ RT reagent (Perfect Real Time ...
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bioRxiv - Molecular Biology 2022Quote: ... Quantitative real-time PCR was performed on a LightCycler 480 II PCR system (Roche, Switzerland) with SYBR Green qPCR Master Mix (TaKaRa Bio) ...
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bioRxiv - Molecular Biology 2022Quote: ... We conducted a PCR reaction using the Expand Long Range PCR Kit (Roche, Basel, Switzerland) and the TuGluCl2in primers ...
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bioRxiv - Microbiology 2023Quote: ... PCR was done with the Kapa HiFi PCR kit (KAPA Biosystems, Cape Town, South Africa) using 5 μl of extracted DNA as template and the following cycling conditions ...
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bioRxiv - Microbiology 2023Quote: ... vector fragments and inserts were amplified by PCR using the KAPA HIFI PCR Kit (Roche) and assembled using a Gibson Mix for 30 min at 50°C ...
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bioRxiv - Molecular Biology 2024Quote: ... maxi- and minicircles were PCR amplified and labelled using PCR DIG Probe Synthesis Kit (Roche). The detection was done using DIG Luminiscent Detection Kit (Roche ...
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bioRxiv - Immunology 2023Quote: ... Whole transcriptome amplification (WTA) was achieved via PCR using KAPA Hifi PCR Mastermix (Kapa Biosystems). The WTA product was purified using Agencourt Ampure beads (Beckman Coulter) ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were blocked for 1 hr at room temperature (RT) in 3% BSA (Roche; 10735086001), 0.05% Tween (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCR reactions were performed using LightCycler 480 SYBR Green I Master kit (Roche-04707516001) on a LightCycler 96 system (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... for 1 hr at RT and incubated with anti-DIG-POD (1:500, 11207733910, Roche) and rabbit anti-RFP (1:1000 ...
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bioRxiv - Plant Biology 2021Quote: ... Gene transcript abundance was quantified by RT-qPCR using FastStart Essential DNA Green Master (Roche) on a Lightcycler 96 (Roche) ...
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bioRxiv - Cancer Biology 2021Quote: ... cell proliferation was measured using Real-time Excelligence system (RT-CES, F Hoffman La-Roche) measured every hour ...
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCR was carried out using a LightCycler 480 SYBR Green I Master Mix (Roche). Rpl32 (LOC5577996 ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were blocked in 1% blocking solution (10 min at RT; Roche Applied Sciences) and then incubated overnight at 4°C with sheep anti-DIG alkaline phosphatase conjugated antibody (1:2,000 ...
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bioRxiv - Cell Biology 2019Quote: ... RT qPCR was performed using the LightCycler® 480 Probes Master mix (Roche Applied Science) on a LightCycler® 480 (Roche Applied Science) ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was then amplified in RT-qPCRs using LightCycler 480 SYBR Green I Master (Roche) and transcript-specific primers on a Roche LightCycler 480 thermocycler following standard protocol (45 cycles ...
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bioRxiv - Plant Biology 2019Quote: ... then analyzed by RT-qPCR using LightCycle FastStart DNA MasterPLUS SYBR Green I kit (Roche) with a LightCycler 480 (Roche ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR assays were performed with SYBR FAST qPCR Master Mix (Kapa Biosystems, Cat# KK4602), using primers indicated in Supplementary Table 2 ...
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bioRxiv - Bioengineering 2022Quote: ... The RT-qPCR reactions were performed using a Roche Diagnostics Lightcycler 2.0 (Roche, Indianapolis, IN), using a protocol previously described in detail42,63 ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed using the LightCycler®480 SYBR Green I Master-Ki (Roche): Primers were used for GAPDH (forward ...
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bioRxiv - Plant Biology 2023Quote: ... was used to perform the RT-qPCR on a Roche LightCycler 96 thermocycler (Roche, USA). The relative gene expression levels were calculated using the 2-ΔΔCT method as described (Livak and Schmittgen ...
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bioRxiv - Cell Biology 2023Quote: ... RT-qPCR mix was prepared using 1X Light Cycler 480 SYBR Green Master (Roche, Germany) and primer pairs at 5uM (Table 2) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... RT-qPCR was performed using a Roche LightCycler® 480 System (Roche Diagnostics, Rotkreuz, Switzerland) thermocycler using qPCR MasterMix No-ROX PyroTaq EvaGreen 5x for HRM (CMB-Bioline ...
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bioRxiv - Genetics 2022Quote: ... 10% of cleared lysate was set aside to serve as input samples and the remainder was incubated at 4°C with anti-GFP antibodies (Roche #11814460001) for 3 h under gentle rotation ...
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bioRxiv - Immunology 2019Quote: ... Individuals were considered to have AITD if they had a positive TPOAb titer (3 times more than the threshold set by the manufacturer [18 IU/mL for Abbott assay and 100 IU/mL for Roche assay]) or had a TSH level more than 10 mIU/L ...
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bioRxiv - Immunology 2021Quote: Single cells were sorted directly into PCR tubes containing 25 μl PCR lysis buffer (1x Buffer 1 from the Expand Long Template PCR System [Roche], 0.5 mg/ml Proteinase K) and lysed for 1h at 55°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The real-time qPCR primers were designed using the universal probe library assay design center (Roche) and UCSC Genome browser (Table 2) ...
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bioRxiv - Plant Biology 2019Quote: ... 0.5 μM of both forward and reverse primers and 1 × FastStart SYBR Green Master mix (Roche). Steady state levels of transcripts were quantified using primers listed in Supplemental Table 2 ...
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bioRxiv - Neuroscience 2020Quote: ... we used gene-specific primers and Light Cycler® 480 SYBR Green I Master (Roche Diagnostics). Polymerase chain reaction conditions were as follows ...