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Citations for Roche :
7301 - 7350 of 7401 citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2020Quote: ... The final pellet was resuspended in 150 μl of NTE buffer (137 mM NaCl, 1 mM EDTA, 10 mM Tris, pH 7.4) with protease inhibitor (Roche, cOmplete Mini tablets, 4693159001). In ultracentrifugation library experiments ...
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bioRxiv - Cell Biology 2020Quote: ... and then digested for 1 h at 37°C in a vigorously shaking solution consisting of 16 mg of Collagenase D (Roche, Pleasanton, CA, USA), 3.0 U of Dispase II (Roche) ...
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bioRxiv - Cell Biology 2020Quote: ... 1% NP-40, 0.5% Na-deoxycholate, 0.1% SDS, 5 mM EDTA, 50 mM NaF, 1 mM PMSF supplemented with Roche 1X Halt Protease inhibitor cocktail), incubated for 1 hour at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were harvested and lysed using immunoprecipitation buffer (25 mM Tris pH 7.4, 150 mM NaCl, 0.4% NP40, 5% glycerol, 1X protease inhibitor cocktail from Roche and 1 mM of PMSF). Approximately 1.5 mg of lysate from each sample was incubated with brachyury antibody at 4°C for 4 h ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were pooled together and resuspended in 1.5 mL immunoprecipitation buffer (25 mM Tris pH 7.4, 150 mM NaCl, 0.4% NP40, 5% glycerol, 1X protease inhibitor cocktail from Roche and 1 mM of PMSF). Cells were then incubated for 30 minutes on ice prior to centrifugation at high speed (15,000 rpm ...
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bioRxiv - Neuroscience 2021Quote: Cells were washed in cold D-PBS prior to lysis on ice using lysis buffer (1% NP40, 100mM Tris pH8, 100mM NaCl, 10% glycerol, 5mM EDTA, cOmplete™ EDTA-free Protease Inhibitor Mixture, Roche Applied Science) supplemented with 20 mM N-ethylmaleimide ...
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bioRxiv - Developmental Biology 2021Quote: ... according to the manufacturer’s instruction and 1 microG of total RNA was reverse transcribed into cDNAs using the first-strand synthesis kit (Roche, Tucson, AZ, USA). Real-time quantitative PCR analyses were performed according to a previously published procedure 39 ...
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bioRxiv - Immunology 2020Quote: ... at 37° C 5% CO2 in 96 well plates in the presence of IL2 (GIBCO 100 IU/mL or Roche 1 ng/mL). Additional conditions included 1 ng/mL TGFβ (GIBCO) ...
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bioRxiv - Cell Biology 2020Quote: ... 10 mM pyrophosphate, 40 mM Hepes pH 7.4, 2.5 mM MgCl2 and 1 tablet of EDTA-free protease inhibitor [Roche] (per 25 ml of buffer)) ...
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bioRxiv - Immunology 2021Quote: ... Lungs were mechanically dissociated by using a scalpel and transferred to collagenase solution consisting of DMEM supplemented with collagenase D (Roche, 1 mg/ml) and DNase1 (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... sections underwent a series of saline sodium citrate buffer washes and were incubated with anti-DIG-AP fab fragments (Roche, 11093274910, 1:1000) O/N at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... and washed once with HK buffer (25mM HEPES pH 7.2, 150mM KCl, 1mM DTT, 1 mM EDTA, Protease Inhibitors (Roche, EDTA-free cOmplete tabTM)) ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatant was aspirated and the pellet was resuspended in a pre-warmed digestion mix containing 1 mg ml-1collagenase/dispase (Roche Diagnostics, Indianapolis, USA) and 10 μg ml-1DNase I (Roche Diagnostics ...
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bioRxiv - Cell Biology 2022Quote: ... Heart tissue was homogenized on ice using Tissue Tearor at medium speed for 1 minute in lysis buffer (RIPA plus 0.1% SDS with Roche EDTA-free protease inhibitor) and allowed to sit on ice 10 minutes followed by sonication using Branson sonifier at 10% amplitude ...
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bioRxiv - Cell Biology 2024Quote: ... 4 s off for 1 min at power 10 microns of amplitude) in ice-cold buffer (50 mM Hepes pH 7.5, 150 mM NaCl, 1 mM DTT, cOmplete (La Roche Ltd., Basel, Switzerland, 05056489001)) and spun down at 13,000 g at 4 °C for 15 min ...
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bioRxiv - Cell Biology 2024Quote: ... Pellet was resuspended in 7 mL of buffer (50 mM Hepes pH 7.4, 150 mM NaCl, 1 mM DTT, cOmplete (La Roche Ltd., Basel, Switzerland, 05056489001)) and subjected to 20 strokes in Dounce homogenizer ...
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bioRxiv - Cell Biology 2024Quote: ... Pellet was resuspended in 7 mL of buffer (50 mM Hepes pH 7.4, 150 mM NaCl, 1 mM DTT, cOmplete (La Roche Ltd., Basel, Switzerland, 05056489001)) and subjected to 20 strokes in Dounce homogenizer ...
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bioRxiv - Genomics 2024Quote: ... the tissue fragments were transferred into a Douncer homogenizer containing a protease inhibitor cocktail (PIC, Roche, 1 tablet in 50 ml of PBS) solution immersed in ice and homogenized using pestles A and B (from less to more plunger adjustment) ...
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bioRxiv - Immunology 2024Quote: ... Brains were homogenized using a glass Potter and digested for 45 min at room temperature (RT) in Hanks’ balanced salt solution (HBSS) medium with collagenase D (1 mg/ml, Roche Diagnostics cat # 11088882001) and deoxyribonuclease (DNase ...
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bioRxiv - Microbiology 2024Quote: ... samples were collected and plasma HIV RNA levels tested by quantitative rRT-PCR (Roche Cobas Ampliprep/Cobas Taqman HIV-1 test, Roche Diagnostics, Mannheim, Germany) at the NICD.
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bioRxiv - Genomics 2023Quote: ... DNA-Seq libraries were prepared from 1 mg of DNA extracted from young leaves using the Kapa LTP library prep kit (Kapa Biosystems, MA, USA). After quantity and quality evaluation with the High Sensitivity chip of a Bioanalyzer 2100 (Agilent Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... both vascular and parenchymal pellets were resuspended in cold 1% NP40 in PBS buffer with protease and phosphatase inhibitors added (Roche 04693159001 and 04906837001). The resuspended pellets were then vortexed for 20 s ...
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bioRxiv - Neuroscience 2022Quote: ... brain sections as previously described (46) (Iba-1 staining was conducted on a Ventana Benchmark using OptiView and UltraView detection kits provided by Roche (Roche Molecular Systems, Inc). Sections were deparaffinized in xylene and rehydrated through an ethanol series ending in distilled water ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were then mounted to glass slides and coverslipped with Vectashield Vibrance (Vector H-1700) Primary antibodies used for immunohistochemistry (IHC) are listed here with dilutions indicated in parentheses: rat anti-HA (Roche 11867423001, 1:500), chicken anti-GFP (Abcam ab13970 1:1500) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein expression was determined by solubilizing 2–4 x 106 cells in lysis buffer (1% Triton X-100, 150 mM NaCl, 0.5 mM EDTA) plus 2X protease inhibitor cocktail (Roche Applied Science, Boulder, CO). Samples were analyzed by SDS-PAGE and Western analysis was performed using either mouse anti-FLAG antibody (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... a left lobe was digested for 1 hour at 37°C in RPMI containing 13 mg/mL DNase I (Roche, Randburg, South Africa) and 50 U/mL collagenase IV (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... then lysed in ice-cold NP-40 lysis buffer (25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% NP-40, 5% glycerol, Roche cOmplete protease inhibitor cocktail) by trituration followed by incubation on ice for 10 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated in anti-DIG POD (sheep anti-digoxigenin peroxidase conjugated antibody, RRID AB_514500; Roche, diluted 1:200 in the blocking buffer) overnight ...
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bioRxiv - Neuroscience 2023Quote: ... Pellets were resuspended in lysis buffer (20mM Tris-HCL pH 8.0, 300mM NaCl; 4ml/100ml culture) with freshly added protease inhibitors (Roche #11697498001: 1 tablet/50ml) and lysozyme (0.1mg/ml) ...
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bioRxiv - Developmental Biology 2023Quote: ... Pelleted nuclei were resuspended in 333 µl of nuclear lysis buffer (50 mM Tris-HCl pH 7.5, 10 mM EDTA, 1% SDS, 1x Roche Complete protease inhibitors cocktail), kept 5 min on ice and diluted with 667 µl of ChIP dilution buffer (16.7 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: RNA was obtained from epithelioids 1 week after medium change to mFAD or from mouse esophageal epithelium peeled and incubated with Dispase I (Roche catalog no. 04942086001) diluted at 1 mg/ml in PBS for 15 min ...
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bioRxiv - Immunology 2023Quote: ... The gut was then added to a 1.5 mL Eppendorf tubed filled with 1 mL pre-warmed 37°C collagenase/dispase solution (Roche 10 269 638 001) and incubated for 90 seconds before adding to a 40-micron filter as above ...
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bioRxiv - Cell Biology 2022Quote: ... 10 mM Na-β-Glycerophosphate and 1% (v/v) TritonX-100 and immediately prior to use the buffer was supplemented with cOmplete™ protease inhibitor cocktail (Roche. Cat# 04693116001).
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bioRxiv - Immunology 2023Quote: Total hemolymph was harvested by bleeding ten L3 wandering larvae in 100 μL of Phosphate Buffer Saline (PBS) supplemented with Complete Inhibitor (Roche, diluted 1/50), PMSF (1 mM ...
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bioRxiv - Genetics 2023Quote: ... Washed cells were carefully resuspended in 570 μL Buffer A with additives (0.2 mM spermidine, 0.5 mM spermine, 1 mM PMSF, ½ cOmplete ULTRA protease inhibitors tablet, Roche, per 5 mL Buffer A) and permeabilized with 0.1% digitonin in 30 ° C water bath for 5 min ...
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bioRxiv - Microbiology 2023Quote: Cell pellets were resuspended in 300 μL of buffer 1 (20 mM K-HEPES pH 7.9, 50 mM KCl, 10% glycerol and Roche EDTA-free protease inhibitors). Samples were sonicated on ice using a probe-type sonicator (4 cycles of 15 s with 15 s resting on ice ...
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bioRxiv - Biophysics 2024Quote: ... The pellet was resuspended in 50 ml lysis buffer (10 mM Tris-HCl, pH 8.0, 1 mM EDTA, Roche Complete protease inhibitor cocktail) and the lysate was boiled for 20 min ...
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bioRxiv - Immunology 2024Quote: ... lungs were minced with scissors and then enzymatically digested for 45 min at 37°C in RPMI-1640 medium supplemented with 1 mg/ml Collagenase D (Roche-Diagnostics, Indianapolis, IN), 1 mg/ml hyaluronidase ...
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bioRxiv - Immunology 2024Quote: ... THP-1-monocyte-derived IFN-γ-primed macrophages were induced by co-stimulation with PMA and 100 U/ml human IFN-γ (Roche, 11040596001) for 24 h ...
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bioRxiv - Synthetic Biology 2024Quote: ... 10 mM Tris-HCl pH 7.5/140 mM NaCl/1% NP-40/1% sodium deoxycholate/0.1% sodium dodecyl sulfate (SDS)) and 150 μL of Complete Mini protease inhibitor cocktail (Roche Diagnostic Systems, Laval, Canada). Harvested cells were sonicated using three 12-second bursts of a Sonic Dismembrator (model 500 ...
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bioRxiv - Neuroscience 2024Quote: ... were homogenized with a pre-chilled Dounce homogenizer using RIPA buffer (50 mM Tris-HCl, 250 mM sucrose, 1 mM EDTA, and Roche protease inhibitor tablet). Tissue lysates were cleared by cold centrifugation ...
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bioRxiv - Neuroscience 2024Quote: NPCs or iPSCs were lysed with HKT buffer (25mM HEPES pH 7.2, 150mM KCl, 1% Triton X-100, 2mM DTT, 1x protease inhibitor (Roche, cOmplete™ EDTA free), 1mM EDTA ...
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bioRxiv - Cancer Biology 2024Quote: ... Crosslinked cells were lysed in 1 ml of Dig-Wash buffer (0.02% digitonin, 20 mM HEPES-potassium hydroxide buffer, pH 7.5, 150 mM sodium chloride, 0.5 mM spermidine, 1 Roche cOmplete EDTA-free tablet (11873580001) per 50 ml buffer and 0.1% BSA ...
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bioRxiv - Neuroscience 2024Quote: Fresh-frozen brain samples were homogenized in 10x volume/weight of lysis buffer (1% NP40 (Thermo 85124) in ice cold PBS with protease and phosphatase inhibitors (Roche 04693132001 and 04906837001)) using a 3 mm bead (Qiagen 69997 ...
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bioRxiv - Cancer Biology 2024Quote: ... A total of 19 hindguts were minced with a blade and incubated for 30 min at 37°C in a solution of Collagenase D (Roche, 1 mg/mL). After filtering in a 40 μM strainer and washing with BGJb ...
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bioRxiv - Cell Biology 2024Quote: ... Cell pellet was resuspended in 700 uL of ice-cold isotonic buffer (20 mM HEPES, pH 7.4, 250 mM sucrose and 1 mM EDTA) supplemented with EDTA-free protease (Roche, cOmplete EDTA-free, 05056489001) and phosphatase inhibitors (Roche ...
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bioRxiv - Genetics 2024Quote: ... Nuclei were extracted by resuspending in 100 μL nuclei extraction buffer per 1-2.5 M cells (20 mM HEPES pH 7.5, 10 mM NaCl, 0.5 mM spermidine, 0.1% BSA, 0.1% NP-40, and 1 tablet Roche mini protease inhibitor per 10-15 mL) and incubating for 10 minutes on ice ...
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bioRxiv - Cell Biology 2020Quote: ... 1% Triton X-100, 150mM NaCl, 50mM Tris pH 7.5, 10mM MgCl2, 5mM NaF, 1mM sodium orthovanadate, and Roche EDTA-free protease inhibitor cocktail). Unstimulated control cells were lysed in solution by adding an equal volume of 2x cold lysis buffer for a final volume of 500 μL ...
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bioRxiv - Neuroscience 2021Quote: ... whole brain samples were homogenized in RIPA lysis buffer supplemented with protease inhibitors (cOmplete mini + EDTA, Roche, 1 mM PMSF, 10 μg/ml leupeptin) and phosphatase inhibitors (10 mM sodium fluoride ...
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bioRxiv - Systems Biology 2021Quote: The digested lysates were mixed 1:1 with 2x IP buffer (200 mM Tris, pH 8.0; 600 mM NaCl; 4% Triton X-100; 2x Roche Complete EDTA-free protease inhibitors), and then kept on ice for no more than a few hours prior to antibody addition ...