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Citations for Roche :
7251 - 7300 of 7401 citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2024Quote: ... Kidney fragments from each replicate was then re-suspended in a normalised volume of extraction buffer (10 mM Tris, 150 mM NaCl, 1% Triton X-100, 25 mM EDTA, Roche protease inhibitor) such that 1 mg kidney/µl solution ...
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bioRxiv - Pathology 2024Quote: ... the aortic sections were incubated overnight at 4°C in a water-humidified chamber with anti-DIG-AP antibody diluted in blocking buffer (Roche, 1:500), followed by a three-time wash with TBST on the next day ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The probe was detected by incubating samples overnight at 4°C with a 1:1500 dilution of Anti-DIG-POD antibody (Roche, Catalog# 11207733910) in Blocking Buffer ...
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bioRxiv - Plant Biology 2024Quote: ... The membranes were blocked in 5% (w/v) nonfat dry milk and probed with anti-HA antibodies (Roche 11867431001; 1:10,000 dilution), followed by horseradish peroxidase-coupled secondary anti-rat antibody (Sigma-Aldrich A9037 ...
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bioRxiv - Pathology 2024Quote: Multicellular BBB organoid arrays were formed for 48 h and consequently pre-incubated with zosuquidar (RO1033295-198-004, Roche Basel, 1 µM) in Endothelial Basal Medium (EBM-2 ...
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bioRxiv - Developmental Biology 2024Quote: ... Digoxigenin- or fluorescein-labeled riboprobes were synthesized as described in previous work (58) and detected with anti-digoxigenin-POD or anti-fluorescein-POD antibodies (1:2000, Roche/Sigma-Aldrich) blocked with 10% heat-inactivated Horse serum and 10% Western Blotting Blocking Reagent (Roche) ...
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bioRxiv - Immunology 2024Quote: ... diluted in 1 mL PBS and homogenized by bead-beating with 1 mm ceramic beads once for 40 s 6000 rpm using a MagNA Lyser (Roche Diagnostics GmbH). After 7 days of C ...
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bioRxiv - Genomics 2024Quote: ... 0.5% N-Lauroylsarcosine) and resuspended in 100 μl of complete LB3 (LB3 containing 1 × Roche cOmplete Mini Protease Inhibitor Cocktail (Roche, Cat.N: 04693159001)) ...
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bioRxiv - Genetics 2024Quote: Paired-end sequencing libraries for QTL-Seq analysis were prepared with >1 µg of DNA using a KAPA HyperPrep PCR-free kit (Roche, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Total RNA was extracted from HCT116 and THP-1 cell lines using the Roche High Pure FFPE Extraction Kit (Roche Life Sciences) and amplified using the NuGen Ovation FFPE Amplification System v3 (NuGen San Carlos) ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1 tablet of protease and phosphatase inhibitors per 50 mL (cOmplete™ ULTRA Tablets Mini EDTA-free and PhosSTOP, Roche Diagnostics) and sonicated with Vibracell (10 cycles ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed in ice-cold 1X PBS before being scraped into 200 µL of ice-cold 1X PBS containing 0.1% NP-40 (1 tablet of the cOmplete Protease Inhibitor Cocktail (Roche, Cat No. 11697498001) and 1 tablet of the PhosSTOP Phosphatase Inhibitor Cocktail (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... 25 µg/ml digitonin and 1 M hexylene glycol) supplemented with protease inhibitor cocktail (cOmplete Mini EDTA-free tablets, Roche, Basel, Switzerland) for 10 min at 7°C rotating ...
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bioRxiv - Bioengineering 2024Quote: ... Specific cell types within these sections were identified using a series of primary antibodies: 1:500 rabbit anti-ERG (Roche, Basel, Switzerland) for endothelial cells ...
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bioRxiv - Cell Biology 2024Quote: ... and 1% Triton X-100) with either 1mM CaCl2 or 1mM EGTA supplemented with complete protease and phosphatase inhibitors (Roche, Mannheim, Germany). Lysates were subjected to two rounds of sonication for 10 s each ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were blocked in 5% milk in PBS with 0.001% Tween-20 (PBST) before incubation with the specified antibodies prepared in 5% milk (GFP (Roche, 11814460001, 1:1,000), FLAG-HRP (Merck ...
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bioRxiv - Evolutionary Biology 2024Quote: ... subsequently resuspended in 200µl of Lysis Buffer (1% SDS, 10 mM EDTA, 50 mM Tris-HCl pH 8, 1x cOmplete™, EDTA-free Protease Inhibitor Cocktail (Roche #04693132001)) and frozen with liquid nitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... pH 8.0 50 mL / 5 M NaCl 30 mL / 5 g Sodium Deoxycholate / 1 g SDS / 1 L SDS g / NP-40 10 mL / MQ up to 1 L) containing 1x protease inhibitor (cOmplete™, EDTA-free Protease Inhibitor Cocktail [Roche, 16829900]). After centrifugation at 10,000 G ...
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bioRxiv - Microbiology 2020Quote: ... bacteria were harvested by centrifugation at 6,000 g for 10 min at 4°C and resuspended in L Buffer (25 mM Tris-HCl, 500 mM NaCl, 10 mM Imidazole, 1 mM PMSF, 5 % Glycerol, pH 8, containing Roche protease inhibitor cocktail). Bacteria were then lysed by sonication ...
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bioRxiv - Cell Biology 2020Quote: Mammalian cell lysates were prepared in radioimmuno-precipitation assay (RIPA) buffer supplemented with 1 tablet of EDTA-free protease inhibitor (Roche, Cat No. C762Q77) per 25ml buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were then quickly washed twice in wash+ buffer (20 mM HEPES pH7.5 + 150 mM NaCl + 0.1% BSA + 0.5 mM Spermidine + 1 large Roche complete EDTA-free tablets in water). Samples were resuspended in 150 μl Wash+C (wash+ + 100 mM CaCl2 ...
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bioRxiv - Developmental Biology 2020Quote: ... Pelleted nuclei were resuspended in 333 μl of nuclear lysis buffer (50 mM Tris-HCl pH 7.5, 10 mM EDTA, 1% SDS, 1x Roche Complete protease inhibitors cocktail), kept 5 min on ice and diluted with 667 μl of ChIP dilution buffer (16.7 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... expressing polymerase module and SII-tagged Mpe1 were lysed in Buffer A (50 mM HEPES pH 8, 75 mM NaCl, 1 mM TCEP) supplemented with 3x protease inhibitor tablets (Roche, cat. No. 11836153001) and 1 ml BioLock ...
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bioRxiv - Genetics 2020Quote: ... After 24 hours cells were then fixed with a 1% (v/v) formaldehyde solution supplemented with cOmplete™ Mini EDTA-free Protease Inhibitor Cocktail (Roche®, UK) for 15 minutes with gentle agitation at room temperature ...
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bioRxiv - Immunology 2021Quote: ... 72°C for 1 min) including the TruSeq primer mix and the KAPA Hifi Uracil+ Poly-merase Master Mix (Kapa Biosystems, Cat. #KK2801). The PCR product was purified and size controlled by Agilent Technologies 2100 Bioanalyzer (High Sensitivity DNA Chip) ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 μm sections from the TMA-2 human adenocarcinoma cohort were duplex stained using a Ventana autostainer with antibodies for cytokeratin AE1/AE3 (1:250, #NCL-L-AE1/AE3, Leica detected with Roche discovery yellow chromogen) and SOX9 (1:10000 ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were pelleted in a centrifuge and lysed with lysis buffer (20mM Tris pH 7.5, 1% Triton, 1mg/mL Lysozyme, 5mM DTT and protease inhibitor cocktail, Roche #11-836-170 001) for 1 hour at 4 °C ...
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bioRxiv - Physiology 2021Quote: 50 islets per sample were washed in PBS twice and then lysed and sonicated in RIPA buffer (10mM HEPES, 50mM β-glycerol phosphate, 1% Triton X-100) supplemented with complete mini protease inhibitor cocktail (Roche, Laval, QC) and phosphatase inhibitors (2mM EGTA ...
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bioRxiv - Physiology 2020Quote: ... Inhibitors were added fresh on the day of use and included 1 cOmplete™ protease inhibitor cocktail EDTA free tablet (Roche, Basel, Switzerland) and Phosphatase Inhibitor Cocktail 3 both purchased from Sigma-Aldrich (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... along with the appropriate antibody (mouse HA 12CA5, 7.5 μl 0.4 mg/ml, Roche; mouse M2 FLAG, 5 μl 1 mg/ml, Sigma), before incubating at 4 °C on a rotating wheel at 14 rpm for 15-18 h ...
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bioRxiv - Microbiology 2021Quote: ... Cells were harvested and resuspended in lysis buffer A (50 mM Tris-HCl, pH 7.5, 300 mM NaCl, 1 mM PMSF, and Roche EDTA free protease inhibitor). Following sonication and centrifugation ...
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bioRxiv - Cell Biology 2021Quote: ... and the cDNA template was digested for 30 min at 37 °C after the addition of 1 µl DNase I (10 U/µl; Roche Diagnostics, Rotkreuz, Switzerland), 5 µl 1M Tris/HCl buffer (pH 8.0) ...
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bioRxiv - Neuroscience 2022Quote: ... incubated with high salt HKT buffer (25mM HEPES pH 7.2, 1M NaCl, 1% Triton X-100, 2mM DTT, 1x Protease Inhibitor (Roche, cOmplete™ EDTA free)) for 10 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... cells were collected and incubated in HKT lysis buffer (25mM HEPES pH 7.2, 150mM KCl, 1% Triton X-100, 2mM DTT, 1x Protease Inhibitor (Roche, cOmplete™ EDTA free)) for 30 minutes on ice prior to homogenization with a 25-gauge syringe needle ...
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bioRxiv - Cell Biology 2022Quote: Cells were washed once with ice-cold PBS and stored at -80 °C or immediately lysed using lysis buffer (50 mM HEPES pH 7.4, 150 mM NaCl, 1% NP-40, 2x Roche EDTA-free protease inhibitors). Lysates were collected by scraping and were cleared by centrifugation at 16,100 g for 10 minutes at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: A total amount of 1 μg total RNA per sample was used for sequencing libraries generation by using KAPA mRNA HyperPrep Kit (KAPA Biosystems, Roche, Basel, Switzerland) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Plant Biology 2021Quote: ... total protein was isolated from Agrobacterium infiltrated leaves using extraction buffer (50mM Tris-Cl, 1% β-Mercaptoethanol and protease inhibitor cocktail (Roche, Basel, Switzerland)) ...
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bioRxiv - Plant Biology 2021Quote: ... pH 7.4) for 30 min and subsequently incubated 1 h with anti-HA-peroxidase high-affinity monoclonal rat antibody (3F10; Roche [catalog no. 12013819001]) diluted 1:1000 in 2.5% (w/v ...
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bioRxiv - Neuroscience 2020Quote: ... followed by mechanical and enzymatic digestion with 150 μL collagenase/dispase (1 mg/mL) and 300 μL DNAse (10 mg/mL; both Roche diagnostics, IN, USA) for 45 min at 37 °C with mild agitation ...
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bioRxiv - Biochemistry 2020Quote: ... the expression medium containing the secreted fusion protein was supplemented with 1/4 tablet of protease inhibitor (complete EDTA-free protease inhibitor cocktail tablets, Roche Diagnostic GmbH, 45148300) and transferred to a 15 ml Falcon tube containing 200 μl of washed ...
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bioRxiv - Biochemistry 2020Quote: ... The spheroplasts were resuspended in ice-cold D-buffer (0.6 M sorbitol, 10 mM Tris-HCl pH 7.4) containing protease inhibitors (complete protease inhibitor cocktail, Roche; 1 tablet per 50 mL), and lysed by 10 strokes in a Dounce homogenizer ...
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bioRxiv - Genetics 2022Quote: ... Samples were suspended in Laemmli sample buffer supplemented with a protease and phosphatase inhibitor cocktail (PIC; 1 mM PMSF, cOmplete™ Protease Inhibitor Cocktail [Roche, Base, Switzerland], PhosSTOP [Roche, Base, Switzerland]), and 10 mM N-ethyl maleimide (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... 2X SSC was replaced with 1X DNase buffer for 5 min and then a 1:50 dilution of DNase I in DNase buffer (DNase I recombinant, RNase-free, Roche, Cat. No. 04716728001), and incubated for 1 hour at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed with cold PBS and resuspended in NE1 buffer (20 mM HEPES-KOH pH7.9, 10 mM KCl, 1mM MgCl2, 1 mM DTT, 0.1% triton X-100 supplemented with Roche complete protease inhibitor EDTA-free) and rotated for 10 minutes at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... The membrane was then incubated in antibody detection solution consisting of 1:20000 anti-HA rat mAb conjugated to horseradish peroxidase (HRP) (Roche [3F10], Cat. # 1201381900) diluted in MTBST 0.05% for 1 hour at room temperature on a tube rotator ...
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bioRxiv - Cell Biology 2022Quote: ... cells were lysed in IP buffer (150 mM NaCl, 10mM Tris-HCl pH 8, 1 mM DTT, 0.2% NP-40, 1x protease inhibitor [Roche], 1x phosphatase inhibitor [Roche]), sonicated and centrifugated for 10 minutes at 14,000 RPM at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed twice using ice-cold D-PBS before scraping in ice-cold RIPA lysis buffer (50 mM Tris [pH 7.4], 150 mM NaCl, 1% NP-40 alternative, complete protease inhibitor and phosphatase inhibitor [Roche Applied Science, Mannheim, Germany]). The lysate was incubated on ice for 30 minutes with gradual agitation before clarification of samples by centrifugation at 10,000 ×g for 10 minutes at 4°C ...
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bioRxiv - Cell Biology 2020Quote: Pellets from exponentially grown cultures of Pea2-9myc expressing yeast cells were resuspended in yeast extraction buffer (50 mM HEPES, 150 mM NaCl, 1 mM EDTA) with 1x protease inhibitor cocktail (Roche Diagnostics, Penzberg, Germany), and vortexed together with glass beads (3-fold amount of glass beads and extraction buffer to pellet weight ...
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bioRxiv - Cancer Biology 2021Quote: ... one 10 cm plate of Hepa1c1c7 cells at 80% confluence was lysed in 1 mL Pierce IP Lysis Buffer (supplemented with 100 U/mL SUPERase-IN and 1X Roche protease inhibitor cocktail) and incubated on ice for 10 min ...
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bioRxiv - Physiology 2020Quote: ... and immunodetection of the DIG-labeled hybrids was performed with a peroxidase-conjugated anti-DIG antibody (1:2000; raised in sheep; Roche Diagnostics, Mannheim, GER). The fluorescence signal was developed with tyramide signal amplification (TSA ...