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Citations for Roche :
7001 - 7050 of 8217 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 30 mM mgCl2 x 6 H2O and 5 mg/mL glucose-6-phosphate dehydrogenase (Roche Diagnostics) in 0.1 M potassium phosphate buffer pH 7.4 ...
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bioRxiv - Molecular Biology 2020Quote: ... 9 µl of qPCR reaction mix containing 5 ul LightCycler 480 Probes Master (Roche, Cat#04707494001), 3.4 ul of Nuclease Free H2O (Ambion ...
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bioRxiv - Biochemistry 2021Quote: ... 5% v/v glycerol) supplemented with HP plus protease inhibitor mix (Serva) and DNase I (Roche). Cell lysates were prepared by sonication and cleared by centrifugation at 80000g at 4°C for 30-45 minutes ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... the medium was added with 10 μL of 5 mg/mL MTT solution (Roche, Basel, Switzerland), followed by adding the formazan diluted in about 200 μL of dimethylsulfoxide (Genview ...
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bioRxiv - Synthetic Biology 2021Quote: ... 5 mM EDTA) containing 1x protease inhibitor cocktail (cOmplete™, EDTA-free Protease Inhibitor Cocktail, Roche). Cell debris was removed by centrifugation (8,000 g ...
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bioRxiv - Molecular Biology 2023Quote: ... and filtered prior to loading onto a prepacked 5 mL cOmpleteTM His-Tag purification column (Roche) equilibrated in HBS ...
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bioRxiv - Microbiology 2023Quote: ... resuspendend in 10 ml of PBS buffer with 5 μg/ml DNaseI (from bovine pancreas, Roche) and 10 μg/ml lysostaphin and incubated for 20 min at 37°C to allow cell lysis ...
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bioRxiv - Neuroscience 2023Quote: ... and 0.2 μl (5 U/μl) FastStart™ Taq DNA Polymerase (Roche Diagnostics GmbH, Mannheim, Germany) in a total volume of 25 μl ...
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bioRxiv - Immunology 2023Quote: ... and equal amounts of protein from lysate were incubated with 5 μg anti-FLAG antibody (Roche) overnight ...
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bioRxiv - Neuroscience 2022Quote: ... and 5 mM EDTA) containing a protease inhibitor cocktail (cOmplete™, Mini Protease Inhibitor Cocktail, Roche) and phosphatase inhibitors (Phosphatase Inhibitor Cocktail 2 ...
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bioRxiv - Microbiology 2024Quote: ... 2.5 μL of 5 μM reverse primer and 10 μL of KAPA SYBR FAST (KAPA Biosystems). The qRT-PCR was done in CFX96 Real-Time System C1000 Touch Thermal Cycler (Bio-Rad) ...
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bioRxiv - Genetics 2024Quote: ... Total RNA was extracted from 5-8 pairs of ovaries using an RNA extraction kit (Roche). 50 ng of total RNA was used to make cDNA using the Transcriptor First Strand cDNA Synthesis kit (Roche ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM EDTA pH 8.0) with 1X complete protease inhibitors (cOmplete Protease Inhibitor Cocktail, Roche 11697498001) by vortexing ...
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bioRxiv - Plant Biology 2022Quote: ... 5 mM imidazole and 500 μL cOmplete Hig-tag purification Resin (obtained from Roche, ref 05893682001). The mixture was gently shaken at 4°C for 4 h in dark ...
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bioRxiv - Molecular Biology 2023Quote: ... or pre-extracted for 5 minutes on ice with CSK/0.5% Triton buffer (supplemented with Roche Complete Protease inhibitor and PhosSTOP Phosphatase inhibitors ...
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bioRxiv - Microbiology 2023Quote: ... After the membrane was blocked with 5% BSA (Bovine Serum Albumin Fraction V, Roche Diagnostics, Germany) in TBST buffer (1X Tris-Buffered saline ...
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bioRxiv - Biochemistry 2022Quote: ... and 63 mM adenosine 5’-triphosphate disodium salt trihydrate brought to pH 7.0 (Roche: ATPD-RO), were stored at −20°C.
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bioRxiv - Cell Biology 2023Quote: ... Each RT-qPCR reaction (10 μl) contained 5 μl of SYBR Green Select Master Mix (Roche), 300 nM of the corresponding forward and reverse primers ...
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bioRxiv - Genomics 2023Quote: ... final extension 5 min at 62 °C) using the KAPA HiFi HotStart Ready Mix (Kapa Biosystems). Amplified libraries were purified ...
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bioRxiv - Cancer Biology 2024Quote: ... tissue sections were thawed at room temperature for 30 min and blocked with 5% BSA (Roche) + 0.2% Tween 20 (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... 5 µl of purified DNA is processed using the KAPA HyperPlus kit (Roche Sequencing, CA, USA) according to manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... 0.3% Tween-20 pH 7.5) for 5 min at 37°C and blocking solution (#11585762001, Roche) for 45 min at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... 10 µl qPCR reactions were used for qPCR: 5 µl SYBR Green I Master Mix (Roche), 0.5 µl of 10 µM forward and reverse primer (final concentration 0.5 µM each primer) ...
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bioRxiv - Biophysics 2024Quote: ... and 5 mM ATP) containing an ATP regeneration system [0.1 mg/mL pyruvate kinase (10109045001, ROCHE) and 2.5 mM phosphoenolpyruvate (860077 ...
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bioRxiv - Cancer Biology 2021Quote: ... Total cell lysates from COS-1 cells 24 hr post transfection were prepared using 300 μl of KAc interaction buffer (Roche Applied Science). GST fusion proteins were bound to glutathione-Sepharose beads (GE Healthcare ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cells were dissociated by incubation in DPBS (without Mg2+ or Ca2+) for 20 min at 37°C and then in 1:200 Liberase TH (Roche, Basel, Switzerland) in DPBS for 20 min at 37°C ...
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: ... Yeast pellets were resuspended in 2 x 500 μl of ice-cold lysis buffer (50mM Hepes, 150mM NaCl, 1% NP-40, 1mM EDTA) containing protease inhibitors (complete Protease Inhibitor Cocktail, Roche Life Sciences), 2 mM phenylmethylsulfonyl fluoride (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... Beads with cells were washed twice in 1 ml Digitonin Buffer (20 mM HEPES-KOH pH 7.5; 150 mM NaCl; 0.5 mM Spermidine; 1x Roche cOmplete™; 0.05% digitonin), and resuspended in 50 µl Digitonin Buffer with addition of 2.5 µl CUTANA pAG-MNase (20x stock) ...
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bioRxiv - Microbiology 2021Quote: ChIP followed by qPCR or Illumina sequencing was performed as described58 using the 3F10 rat anti-HA antibody (1:500; Roche no. 11867423001) to immunoprecipitate HA tagged AP2-HS ...
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bioRxiv - Molecular Biology 2021Quote: ... MGC803 cells were harvested and used to prepare lysate with cell lysis buffer with 1× Protease Inhibitor Cocktail (REF04693159001, Roche, Mannheim, Germany). The lysate was incubated with Glutathione Sepharose beads (20 μL for one group ...
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bioRxiv - Developmental Biology 2020Quote: ... Lysates were made from 1∼2 mL packed worms in lysis buffer (25 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% NP-40, 10% glycerol, 1x cocktail of protease inhibitors from Roche (Complete, EDTA free), 40 mM NaF ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The final PBMC pellet was taken up in 200 μl lysis buffer (PBS + 1% Triton-X100 + Complete Mini protease inhibitor cocktail tablet (Roche, Mannheim, Germany)) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Tissues were homogenized in 1 ml N-PER (brain) or T-PER (other organs) + Complete Mini protease inhibitor cocktail tablet (Roche, Mannheim, Germany) in FastPrep Lysing Matrix D tubes on a FastPrep homogenizer ...
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bioRxiv - Cell Biology 2022Quote: ... minced into ∼1-mm pieces and sequentially digested twice for 30-min in endotoxin-free collagenase (Liberase; Roche Diagnostics GmbH, Mannheim, Germany) to obtain osteocyte-enriched bone samples ...
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bioRxiv - Microbiology 2020Quote: ... 1 mL digestion mix (4.8 mg/ml Collagenase IV; Worthington Biochem, and 200 μg/ml DNase I; Roche Diagnostics, in 1x PBS) was added after which the tissue was incubated at 37°C for 45 min ...
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bioRxiv - Molecular Biology 2021Quote: ... and amplified using a reporter specific forward primer with illumina sequencing adaptors and a reverse primer binding the partial illumina read 1 sequence with the remaining sequencing adaptors using Kapa HiFi HotStart DNA Polymerase (Kapa Biosystems, #KK2601) using 18X cycles for GFP and 23X for Firefly reporter ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Cells in 6-well plates were lysed with RIPA lysis buffer containing 1mM PMSF and 1% phosphatase inhibitor cocktail (PhosSTOP, Roche group, Swiss). The supernatant was collected after centrifuging at 14,000 rpm for 15 min at 4℃ ...
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bioRxiv - Biochemistry 2020Quote: ... The gel piece was vacuum dried and treated with trypsin (15 ng/μl in 25 mM ammonium bicarbonate, 1 mM CaCl2; Promega or Roche sequencing grade) at 37°C for 16 hours ...
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bioRxiv - Bioengineering 2021Quote: ... Total RNA of 1 μg from each sample was reverse transcribed with random hexamers using Transcriptor First Strand cDNA Synthesis Kit (Roche Diagnostics, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... and the presence of enzymes were confirmed by immunoblot using the anti-His6 antibody conjugated to peroxidase (BMG–His-1 monoclonal antibody) (Roche, Mannheim, Germany).
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bioRxiv - Biophysics 2021Quote: ... Pellets were resuspended in lysis buffer (1 M NaCl, 20 mM Tris, 20 mM imidazole, Roche EDTA-free protease inhibitor, pH 7.5) and lysed by sonication ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 106 cells were lysed for 15 min on ice in 100 μL pre-chilled lysis buffer (50 mM Tris, pH 8, 150 mM NaCl, 1% Triton-X100, 1x cOmplete EDTA-free protease inhibitor cocktail [Roche, Basel, Switzerland]). Lysates were pelleted at 20,000 x g for 15 min at 4 °C ...
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bioRxiv - Plant Biology 2021Quote: ... and digested into peptides using one round of overnight incubation at 37°C with 1:50 (enzyme:protein) trypsin (Roche, Cat. No. 03708969001) and a second round of incubation for 4 hours at 37°C with trypsin and Lys-C (Wako Chemicals ...
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bioRxiv - Neuroscience 2020Quote: Cells were washed with ice-cold PBS and lysed in RIPA buffer (50 mM pH 8.0 Tris-HCl, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 1mM NaVO4, 1mM NaF, Roche complete protease inhibitor tablet). Lysates were cleared by centrifuging at 16,000 Xg at 4°C for 10 m ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse hind limb muscles (tibialis anterior, quadriceps and gastrocnemius) were minced and incubated in a 1 mg/ml collagenase/dispase solution (Roche, Basel, Switzerland) with agitation at 37°C for 1 hour ...
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bioRxiv - Developmental Biology 2021Quote: ... Revelation of the dig-labeled ISH probe was then performed using an anti-Digoxigenin antibody coupled to POD (11207733910; Roche, 1/400) and revealed using a FITC-tyramide (1/400) ...
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bioRxiv - Biophysics 2022Quote: ... and mixed with 1 mL Ni Sepharose 6Fast Flow (Cytiva, Tokyo Japan) (MinD) or cOmplete His-Tag Purification Resin (Roche, Basel, Switzerland) (MinE and msfGFP-MinC) ...
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bioRxiv - Developmental Biology 2022Quote: Single color staining of embryos hybridized with DIG-labelled riboprobes was performed using anti-DIG antibody conjugated to alkaline phosphatase (Roche, 1:6000) and BM purple (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were incubated overnight with 1:1,000 diluted alkaline phosphatase-conjugated anti-DIG sheep antibody (11-093-274-910; Roche Diagnostics, Basel, Switzerland) and then reacted with 0.375 mg/mL nitroblue tetrazolium and 0.188 mg/mL 5-bromo-4-chloro-3-indolylphosphate (NBT/BCIP ...
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bioRxiv - Microbiology 2022Quote: ... by the capillary method and hybridised with digoxigenin-labelled blaOXA- 58 and blaNDM-1-specific probes with an NBT/BCIP colour detection kit (Roche, Mannheim, Germany) according to the manufacturer’s instructions ...