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Citations for Roche :
6801 - 6850 of 8217 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... one volume of 2X TBS buffer supplemented with 1 mM PMSF and cOmplete EDTA-free protease inhibitor cocktail (Roche Applied Science) was added to one volume of packed worms then added dropwise to liquid N2 to obtain frozen worm ‘popcorn’ ...
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bioRxiv - Cell Biology 2022Quote: ... Nuclei pellet was then resuspended and incubated in 1 mL of nuclear lysis buffer (50mM Tris-HCL pH8.0, 100mM EDTA, 1% SDS, 1mM PMSF, SIGMAFAST™ protease inhibitor cocktail, and Roche PhoStop) on ice for 30 min and 3 freeze-thaw cycles to release chromatin ...
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bioRxiv - Molecular Biology 2022Quote: Cryo-lysed cells were resuspended in lysis buffer (40mM Tris pH 8.0, 200mM sodium citrate) with 1× complete Mini EDTA-free protease-inhibitor solution (Roche Life Science). After centrifugation at 14,000×g for 10 min at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... brains homogenized in 1xPBS containing 1% SDS and protease inhibitors (Complete EDTA-free Protease Inhibitor cocktail, Roche, Sigma Aldrich, Cat#11873580001) and boiled for five min ...
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bioRxiv - Developmental Biology 2022Quote: ... and 1 µg of 330-BFP-CYP3A4-enhancer-R-sgRNA were transfected with X-tremeGENE 9 DNA transfection reagent (Roche 6365809001). After 3-5 days ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by rabbit anti-Rat (AI-4001, Vector) at 1:500 for 32 min and OmniMap™ anti-Rb HRP (760-4311, Roche). Blocking was performed using casein (760-219 ...
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bioRxiv - Biochemistry 2022Quote: ... and subsequent addition of 50 µl lysis buffer supplemented with 1 mM PMSF / ml buffer and 22 µl / ml buffer protease inhibitor cocktail (Complete, Roche Diagnostics). The cell samples were boiled for 3 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... and 10% sheep serum] and incubated with a 1:2000 dilution of alkaline phosphatase-conjugated anti-digoxigenin antibody (Roche Cat# 11093274910) in MABT/Block at 4°C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... AML blast cells were incubated in 1 ml of ice-cold 0.1 M Na2CO3 containing complete protease inhibitor (Roche, Penzberg, Germany) and phosphatase inhibitor PhosSTOP (Roche ...
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bioRxiv - Neuroscience 2021Quote: ... proteinase K treatment was omitted and color development of alkaline phosphatase activity was carried out for 1 h with nitroblue tetrazolium chloride (Roche Diagnostics) and 5-bromo-4-chloro-3-indolyl phosphate (Roche Diagnostics ...
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bioRxiv - Microbiology 2020Quote: ... The pellet of 100 mL was resuspended in 1 mL cold fractionation buffer (40 mM HEPES, 0.1 mM EDTA, 1x cOmplete™ Protease Inhibitor Cocktail (Roche Sigma)) and the pellet of 1 mL lysed with Bug Buster (pellet sample ...
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bioRxiv - Neuroscience 2020Quote: ... 1 μM benzyloxycarbonyl (Z)-Leu-Leu-Leucinal (protease inhibitor, synthesized in-house) and Complete EDTA-free Protease Inhibitor (Roche, cat# 05056489001) in 50 mm MES pH 6.5 with or without 10 μM thiorphan (neprilysin-specific inhibitor ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Viral supernatants were prepared by transfecting 293T cells with GFP- or ABCG-targeting shRNAs encoded in pLKO.1 vectors using X-tremeGENE 360 transfection reagent (Roche, Germany) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... and tissue homogenization was performed using a Bullet Blender in RIPA buffer supplemented with 1% cOmpleteTM protease inhibitor cocktail (Roche Diagnostics), 1% phosphatase inhibitor cocktail 2 (#P5726 ...
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bioRxiv - Genetics 2022Quote: ... Samples were suspended in Laemmli sample buffer supplemented with a protease and phosphatase inhibitor cocktail (PIC; 1 mM PMSF, cOmplete™ Protease Inhibitor Cocktail [Roche, Base ...
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bioRxiv - Pathology 2022Quote: ... labeling probe (359 bp) was synthesized using primers TiPV-Fq and TiPV-NS1-R (Table 1) and DIG-labeling mix reagent (Roche, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed with cold PBS and lysed using buffer A (PBS, 20 mM imidazole, 1% Triton X-100, and a cOmplete® protease inhibitor tablet (Roche). Lysates were clarified for 30 min at 13,000 g at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... the lysed cells were resuspended in LB2 (10 mM Tris-HCl, pH 8.0, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, Roche cOmplete protease inhibitors) and incubated at 35 rpm for 5 min followed by centrifugation ...
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Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: ... sample pellets were resuspended in 200 µL lysis buffer (100 mM triethylammonium bicarbonate buffer (TEAB) with 1% (v/v) sodium deoxycholate (SDC) and 1x protease inhibitor cocktail solution (Roche, Switzerland)) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Endogenous peroxidase activity was blocked by incubation with 3% H2O2 for ten minutes followed by two washes with PBS containing 0.1% Tween-20 and blocking with PBS containing 1% bovine serum albumin (Roche, Mannheim, Germany) and 0.2% skim milk powder (Easis ...
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bioRxiv - Pathology 2020Quote: ... Coverslips were incubated overnight at 4 °C with a 1:100 dilution with one of the following primary antibodies: HA (Roche, #867423001), KDEL (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... and subjected to a protease protection assay by resuspension in 250 μl of freshly prepared 1 mg/mL Pronase (Roche, 10165921001)/0.01% Saponin/PBS at 12°C for 1h ...
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bioRxiv - Molecular Biology 2020Quote: ... rocked at 4°C for 10 min and after centrifugation resuspended in 10 ml ice cold buffer LB2 (10 mM Tris-HCl pH 8.0, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 1x Roche cOmplete protease inhibitors), rocked at 4°C for 5 min and centrifuged again ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μl of each unpurified amplicon was then carried to a second PCR reaction using KAPA HiFi Library Amp (Roche Sequencing). NEBNext Multiplex Oligos (New England Biolabs ...
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bioRxiv - Physiology 2021Quote: ... Collagen was then extracted from the tissue samples using a salt extraction buffer (1 M NaCl, 25 mM EDTA, 50 mM Tris-HCl, pH 7.4) containing protease inhibitors (Roche, Basel, Switzerland). Samples were extracted overnight at 4°C with agitation ...
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bioRxiv - Neuroscience 2020Quote: ... After centrifugation, cells were lysed (750 mM NaCl, 10 mM Tris, 1 mM EDTA pH 7.6, protease inhibitor (Roche, Mannheim, Germany)) ...
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bioRxiv - Genomics 2021Quote: ... Four PCR reactions were then carried out with 1 μl of the purified DNA using the KAPA HotStart HiFi Ready Mix (KAPA Biosystems) for 10 cycles inserting a 15 nt homology sequence for the subsequent cloning step (IF_fwd/IF_rev) ...
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bioRxiv - Immunology 2021Quote: ... and were enzymatically digested in a shaker incubator at 37°C for 40 minutes in RPMI medium containing 1 mg/ml Collagenase D (Roche-Diagnostics), 1 mg/ml hyaluronidase and 50 U/ml DNase I (Sigma Aldrich) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... For large-scale purification cells were resuspended in lysis buffer (50 mM NaPO4 pH=7.0; 250 mM NaCl; 1 tablet complete protease inhibitor EDTA free (Roche, Basel, Switzerland) per 50 mL ...
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bioRxiv - Physiology 2021Quote: ... containing cOmplete EDTA-free protease inhibitor cocktail at a concentration of 1 tablet/7 ml (Roche Applied Science, Indianapolis, IN, USA)) by repeated passage through a 20-gauge needle to obtain plasma membrane-enriched preparations ...
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bioRxiv - Physiology 2020Quote: ... was homogenized by probe sonication in ice-cold membrane buffer (50 mM Tris-HCl pH 7.4, 1 mM EDTA, 250 mM sucrose, and complete protease inhibitors table (Roche Diagnostics, A32965)) ...
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bioRxiv - Microbiology 2021Quote: ... the beads were collected on a magnetic stand for 2 min and washed four times with lysis buffer and four times with wash buffer (50mM Tris pH 7.6, 300mM NaCl, 1% Triton X-100, + complete protease inhibitor/ Roche Cat# 11836153001). The beads were resuspended in 1x Laemmli sample buffer ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... for 3-4 hours at room temperature followed by overnight incubation at 4°C in anti-DIG-AP Fab fragments (1:5000) (Roche 1093274). Embryos were washed with PBST followed by an alkaline tris buffer ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were incubated in 1% Triton X-100 for 30 min on ice followed by treatment with proteinase K (Roche 3115879001) at the indicated concentrations at 37°C for 5 min ...
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bioRxiv - Cell Biology 2020Quote: ... Fusion was carried out by resuspending the mixed cell pellet slowly in 1 mL of 50% PEG 1500 (Roche, Basel, Switzerland) at 37°C over the course of 1 minute and incubated for a further 1 minute at 37°C ...
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bioRxiv - Cell Biology 2020Quote: Cells were lysed in lysis buffer (50 mM Tris-HCl, pH 7.8, 150 mM NaCl, 1% Nonidet-P40 and protease inhibitor cocktail tablets (Roche, Basel, Switzerland)) ...
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bioRxiv - Microbiology 2020Quote: Frozen fecal samples were diluted in lysis buffer [100mg feces per 1 ml of PBS containing protease inhibitors (Roche, Indianapolis, IN)] ...
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bioRxiv - Molecular Biology 2021Quote: ... Planarians were treated with 5% NAC for 5 minutes and 4% fixation for 20 minutes at room temperature followed by bleaching overnight and treated with proteinase K (1 µg/ml, Roche, 03115844001). After 2 hours of blocking with 1% BSA ...
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bioRxiv - Immunology 2020Quote: Skin biopsy specimens were collected and minced before digestion in 1mL of DMEM with 1 mg/ mL collagenase D (Roche Diagnostics) for 3 hours at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: Cos-1 cells were grown in DMEM supplemented with 10% heat inactivated FCS and transfected with Fugene HD (Roche Applied Science) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... 2 mg/ml bovine serum albumin dissolved in TBST) for 1 h and subsequently incubated with anti-DIG-Fab antibody coupled to alkaline phosphatase (Roche: 11093274910) in blocking solution (1 in 5000 ...
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bioRxiv - Genomics 2021Quote: ... Pelleted cells were resuspended in 1 ml of Dig-Wash buffer (0.02% digitonin, 20 mM HEPES-KOH, pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 1 Roche Complete tablet -EDTA (11873580001) per 50 ml buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... Riboprobes were hybridized for 16 hr at 65°C and embryos were incubated with anti-DIG-AP (Roche, dilution 1/4000) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... 1 MM EDTA) supplemented with a cocktail of protease inhibitors (Complete Protease Inhibitor without EDTA, Roche Applied Science, Indianapolis, IN, USA) and phosphatase inhibitors (Phosphatase Inhibitor Cocktail 3 ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were incubated with 1:10 TdT buffer for 10 min and subsequently in reaction mixture [0.12% v/v TdT (3333574001, Roche applied science); 0.25% v/v dUTP (11093070910 ...
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bioRxiv - Immunology 2023Quote: ... 1 mM NaF) with EDTA-free protease inhibitors (cOmplete™ Tablets) and phosphatase inhibitors (PhosSTOP™, both Roche Diagnostics GmbH, Germany) for 1 hour at 4°C under rotation ...
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bioRxiv - Biochemistry 2022Quote: ... Total RNA-seq libraries were then prepared using 1 µg of RNA from each sample and the KAPA RNA HyperPrep Kit with RiboErase (Roche KR1351) according to the manufacturer’s protocol with 10 amplification cycles ...
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bioRxiv - Microbiology 2023Quote: ... cecal and colonic tissues were homogenized in 1 mL of PBS −/− supplemented with the cOmplete EDTA-free protease inhibitor cocktail (Roche Diagnostics). Supernatants were collected by centrifuging the homogenate at 16,000 ×g at 4 °C for 20 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... The minced tissue is lysed in tissue lysis buffer (50 mM Tris-HCl [pH7.5], 150 mM NaCl, and 1% NP-40 (11332473001, Roche Applied Science)) for 15 minutes ...
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bioRxiv - Systems Biology 2023Quote: ... per sample were washed three times with 1 ml wash buffer (20 mM HEPES at pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, and 1 × Roche complete protease inhibitor) at room temperature and resuspended in 225ul of wash buffer ...