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Citations for Roche :
6901 - 6950 of 8217 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed in SDS lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris-HCl pH 8) supplemented with Proteinase Inhibitor Cocktail (Roche, 11697498001) and chromatin was sonicated using the Bioruptor pico (Diagenode ...
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bioRxiv - Neuroscience 2023Quote: ... 300,000 cells were washed twice (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM spermidine, 1× Roche cOmplete protease inhibitors) and attached to 10 ConA-coated magnetic beads (Bangs Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... a 6mm section of the infarcted brains was dissected and homogenised to 300mg wet weight of tissue per 1ml of Lysis Buffer (TBS +1% Triton X-100; Roche Australia).
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bioRxiv - Physiology 2023Quote: ... Then the heart was perfused with the same solution containing 1 mg/mL collagenase Type A (Catalog # 9036-06-0; Roche, Germany) and 0.08 mg/mL protease Type XIV (Cat log # 48655321 ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial lysis and protein digestion were performed simultaneously by adding 50 µL of 1 mg/mL trypsin (Roche Diagnostics, Mannheim, Germany) prepared in a 150 mM NH4HCO3 solution ...
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bioRxiv - Immunology 2023Quote: ... Wells were pre-coated with 100 μL per well of capture anti-histone antibody contained in the Cell Death Detection ELISA kit (1:40 in 1x coating buffer; Roche 11544675001) or anti-myeloperoxidase (MPO ...
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bioRxiv - Microbiology 2023Quote: ... mycelia were resuspended in lysis buffer (10 mM Tris-HCl pH 8.0, 50 mM NaCl, 15 mg/mL lysozyme, 1× protease inhibitor (Roche, Bavaria, Germany)) and incubated at 37°C for 20 min ...
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bioRxiv - Cell Biology 2023Quote: ... CDMs were washed with PBS and treated for 1 h at 37°C with a buffer of 50 µg/ml DNaseI (Roche, #11284932001), 5 mM MgCl2 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed in PBS, incubated in 200ul lysis buffer (50mM Hepes pH 7.4, 150mM NaCl, 1mM MgCl2, 1% NP40, phosSTOP (Roche, Cat# 4906845001), protease inhibitor (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed in PBS and lysed in lysis buffer (150 mm NaCl, 1% IGEPAL CA-630, 50 mm Tris-HCl, pH 8.0, cOmplete EDTA-free Protease Inhibitor Mixture, Roche Applied Science) supplemented with 20 mm N-ethylmaleimide (NEM) ...
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bioRxiv - Genetics 2024Quote: ... Hybridizations of the probe with the RNA were detected with an alkaline phosphatase-conjugated antibody (1:5000; anti-DIG-AP, Fab Fragments, Roche, 11093274910). Finally ...
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bioRxiv - Cell Biology 2024Quote: ... samples were covered with the staining mixture at 37°C for 1 h following instructions of the In Situ Cell Death Detection Kit (Roche, 11684795910). After the TUNEL reaction ...
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bioRxiv - Cell Biology 2024Quote: Samples were lysed in ice cold radioimmunoprecipitation assay (RIPA) buffer containing 1:10 PhosSTOP™ (#4906845001, Roche Diagnostics, Indianapolis, IN, USA) and 1:20 cOmplete™ ULTRA Protease Inhibitor Cocktail (#06538304001 ...
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bioRxiv - Systems Biology 2024Quote: ... and 10 µL (1 µg) was used as input to a second strand synthesis reaction with 2x KAPA HiFi Master Mix (Roche, #07958935001) and an equimolar amount of primer P276R (Table S5) ...
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bioRxiv - Microbiology 2024Quote: ... immunoblotting was performed and HA-tagged SopB was detected using a HA-specific antibody (diluted 1:1000, Roche, Cat. No.: 1867423).
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bioRxiv - Cell Biology 2024Quote: ... The protein samples were separated with SDS-PAGE gel and detected with monoclonal anti-GFP antibody (1:1,000 dilution; 11814460001; Roche, Mannheim, Germany), monoclonal anti-Myc antibody (1:500 dilution ...
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bioRxiv - Cell Biology 2024Quote: ... Beads were then washed three times with 1 mL of lysis buffer with EDTA-free protease-inhibitor cocktail (Roche, Indianapolis, IN), and then three times with 1 mL of lysis buffer without NP-40 or protease-inhibitor cocktail ...
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bioRxiv - Microbiology 2024Quote: ... protein extracts from the respective parasite lines at different stages were analyzed by SDS-PAGE and immunoblotting using anti-GFP mAb (1:1000, Roche, 11814460001), anti-APEX2 antibodies (1:1000 ...
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bioRxiv - Genomics 2024Quote: ... heat-induced epitope retrieval was performed by boiling the sections for 48 minutes with Cell Conditioning 1 buffer (DISC CC1 RUO, Roche, 6414575001) followed by incubation with DISC inhibitor (Roche ...
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bioRxiv - Immunology 2024Quote: ... A total of 100 pmol siRNA was transfected into 1×106 BmN cells using Roch-X gem (Cat:6366236001; Roche, Switzerland). siRNA-NC was used as the negative control ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples subjected to colorimetric signal detection were incubated in anti-Digoxygenin-AP antibody solution (1:4000 dilution; Roche Diagnostics, Mannheim, Germany) overnight while rocking at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... In total of 1 μg total RNA was used for first-strand cDNA synthesis using Transcriptor First Strand cDNA synthesis kit (Roche, Switzerland) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue was washed two times before either storing at -80°C or immediately lysed in SDS lysis buffer (50mM Tris Tis pH 8.1, 10mM EDTA, 1% SDS, cOmplete EDTA-free Protease Inhibitor Cocktail, Roche, Cat# 05892791001) on ice for 15 minutes and subjected to sonication for 4 separate cycles of 30s on and 30s off cycles at high setting (Bioruptor Plus ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated at room temperature for 30 minutes with HRP-labeled sheep anti-FITC antibody (1/500 in TNB, Roche/Sigma). Following washes in TNT ...
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bioRxiv - Pathology 2024Quote: 150 mg wet weight human lung tissue from IPF patients and healthy controls was homogenized in 1 mL ice cold RIPA buffer (defined previously) with complete mini protease inhibitors (Roche 11836153001). Supernatants were collected following centrifugation (10,000 x g for 20 min x 2) ...
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bioRxiv - Pathology 2024Quote: ... and 1 drop of blood was used for direct blood glucose measurements using an Accu Chek Aviva glucose meter (Roche Diagnostics). For 2 of the non-treated and 3 of the anle138b-treated Ob/Ob mice ...
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bioRxiv - Pathology 2024Quote: ... and 1 drop of blood was used for direct blood glucose measurements using an Accu Chek Aviva glucose meter (Roche Diagnostics). During all 3 ISTs of the hIAPP Ob/Ob mice (i.e ...
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bioRxiv - Molecular Biology 2024Quote: ... Sections were blocked in 10% sheep serum and incubated overnight at 370C with anti-DIG-AP antibody (Roche at 1:1000). Signal detection was done using NBT/BCIP substrate (Roche).
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bioRxiv - Neuroscience 2024Quote: Human motor neuron lysates were prepared by resuspending 30 million cells in 1 ml of PBS with protease inhibitors added (Roche, 4693132001). Cell suspension was put through freeze thawing cycle thrice to lyse the cells ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: 200×106 A2780 cells were resuspended in a 2.5 ml lysis buffer (1 ml protease inhibitor 25x (cOmplete™ EDTA-free Protease Inhibitor Cocktail, Roche, Switzerland), 1.5 ml 1x PBS) ...
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bioRxiv - Genetics 2024Quote: ... The resultant pellet was homogenized in three volumes times the heaviest sample of 1% Triton™ X-100 in TBS plus complete protease and phosphatase inhibitors (Roche) and centrifuged at 186 000 g at 4°C for 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... Tissues were resuspended in 900 µL of SDS Lysis buffer (50mM Tris pH 8.1, 10mM EDTA, 1% SDS, cOmplete EDTA-free Protease Inhibitor Cocktail, Roche, #cat 05892791001) and sonicated for four cycles of 10 minutes (30 seconds ON 30 seconds OFF ...
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bioRxiv - Biophysics 2024Quote: ... The PCR cycle was determined for each library using 1/10 of the adaptor-ligated DNA as the template with the KAPA SYBR FAST qPCR Master Mix (Roche, KK4603) by taking the Ct that reaches 1/2 of the maximum intensity and subtracting 2 cycles (Ct -2 cycles) ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.5% (w/v) DOC and 0.1% (w/v) SDS) in the presence of protease (1:40 cOmplete™ Protease Inhibitor Cocktail (Roche, #11836145001)) and phosphatase inhibitors (1:100 Phosphatase inhibitor cocktail 2 (Sigma Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... 105 g cell pellet was collected and resuspended in 1.5X pellet volume of lysis buffer (50 mM Tris-HCl, pH 8.0, 1 mM EDTA, Protease inhibitor Roche cOmplete mini tablet) and lyzed by French press (1200 Psi.) ...
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bioRxiv - Bioengineering 2024Quote: ... The membrane was blocked in 5% milk in PBS with 0.1% Tween 20 (PBS-T) for 1 h at room temperature (RT) before being probed with either rat anti-HA-tag antibody (Roche, 11867423001) diluted 1:1000 in 5% milk in PBST ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were amplified with target-specific primers (see Supp. Table 1 for primersequences) using a LightCycler 480 II real time PCR system (Roche Diagnostics) as previously described (22).
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bioRxiv - Cell Biology 2024Quote: ... Samples were analyzed by standard SDS-PAGE and Western blotting using the following primary antibodies: anti-c-Myc (1:1000, Roche 11667149001), anti-HA (1:1000 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Binding reactions were carried out in wash buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM DTT, EDTA-free protease inhibitors Roche, 2.5 % glycerol) in a total volume of 15 μL with purified protein and 77.3 nM DNA ...
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bioRxiv - Developmental Biology 2024Quote: ... The DIG hapten was detected by incubating the samples in a 1:4,000 dilution of anti-DIG-rhodamine antibody (Roche, Basel, Switzerland) in PBS-T for 90 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Control siRNA (qiagen) and CXCR4 siRNA (SMARTPool, Dharmarcon) were diluted in DOTAP (1,2-dioleoyl-3-trimethylammonium-propane; Roche Applied Sciences). The mix was gently mixed and incubated at room temperature for 15 min ...
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bioRxiv - Molecular Biology 2022Quote: In vitro transcribed RNA was prepared from linearized plasmid containing the human PTH 3’-UTR (68) using a Biotin RNA Labeling Mix (Roche) and T7 RNA polymerase ...
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bioRxiv - Molecular Biology 2020Quote: ... were generated by transfection of 3×FLAG-tagged LRRK2 (WT) plasmid followed by pharmacological selection using an antibiotic G-418 (Roche). Transfection of plasmids and siRNA was performed using Lipofectamine 3000 (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... samples were incubated with 27 µl labeling solution plus 3 µl enzyme solution (In Situ Cell Death Detection Kit, AP, Roche) at 37°C overnight ...
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bioRxiv - Biochemistry 2020Quote: ... The thorax and the abdomen were then washed with 3 ml of pre-chilled 0.1 M KBP pH 7.4 homogenization buffer (HB) containing 1x protease inhibitor (Roche Complete ULTR). The complex was homogenized in 20 ml of HB using 40 ml glass Dounce homogenizer with a loose B pestle (Wheaton Science ...
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bioRxiv - Genetics 2020Quote: ... These pieces were then added to the 3-4 ml of digestion media containing 0.1 mg/ml liberase (Roche, 501003280), 100 U/ml DNase I (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: Aag2 cells were transfected with a plasmid expressing 3×flag tagged Zuc using X-tremeGENE HP DNA Transfection Reagent (Roche), and fixed 48 hours after transfection ...
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bioRxiv - Molecular Biology 2021Quote: A 3×flag tagged Zuc expression plasmid was transfected into Aag2 cells using X-tremeGENE HP DNA Transfection Reagent (Roche). Cells were lysed and lysates incubated with M2-Flag beads (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... after lysis of the insect cell pellet by 3 cycles of freeze-thaw in the presence of Complete protease inhibitor cocktail (Roche), and removal of debris the lysate was loaded onto a 20-40% sucrose density gradient ...
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bioRxiv - Developmental Biology 2020Quote: ... 5 pmol of the forward and reverse gene-specific primers each (Supplemental Table 3) in Light Cycler SYBR Green I Master mix (Roche) on LightCycler 480 II (Roche) ...