Labshake search
Citations for Roche :
7151 - 7200 of 8217 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... then lysed on ice for 15 minutes in non-denaturing lysis buffer (150 mM KCl, 10 mM MgCl2, 5mM HEPES, 1% IGEPAL) supplemented with protease inhibitors (Roche Complete EDTA-free, Roche, Laval, Canada). The samples containing lysis buffer were then collected and spun down for 5 minutes in pellet the cell debris ...
-
bioRxiv - Microbiology 2023Quote: ... 50 mM Tris-HCl, 10% glycerol, 1% IGEPAL [Sigma, St Louis, MO], and cOmplete EDTA-free protease inhibitor cocktail [Roche] [pH 7.4]). Cells were vortexed ...
-
bioRxiv - Molecular Biology 2023Quote: ... Viral loads were measured at all study visits using the Roche Cobas Taqman HIV-1 Test v2.0 (Roche Diagnostics, Branchburg, NJ, USA). CD4 T cell counts were also enumerated at all study visits using the 4-colour MultiTEST/Trucount assay (Becton Dickinson ...
-
bioRxiv - Molecular Biology 2023Quote: ... were sequenced in the forward direction on 1/8 plate of a 454 Life Sciences Genome Sequencer FLX machine (Roche, Branford, CT) using the Macrogen facilities (South Korea).
-
bioRxiv - Developmental Biology 2023Quote: ... The qPCR was performed using PerfectStartTM Green qPCR Super Mix (TransGen Biotech, 1) on a Real-time PCR Detection System (Roche, LightCycle480 II). RPLP0 served as an internal control ...
-
bioRxiv - Microbiology 2023Quote: ... Proteins were eluted with 12 ml of elution buffer (20 mM Tris, 500 mM NaCl, 6 M guanidine, 600 mM imidazole, 1 mM MgCl2 and 1X Roche protease inhibitor) and exchanged into #1 to #5 dialysis buffers in order to remove the imidazole and the Guanidine ...
-
bioRxiv - Genomics 2023Quote: ... 3 million crosslinked cells were resuspended in 1 mL of ice-cold Hi-C lysis buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 0.2% NP-40, and 1× Roche protease inhibitors Cat#11697498001) and rotated at 4 °C for 30 minutes ...
-
bioRxiv - Cell Biology 2022Quote: ... and lysed in immunoprecipitation buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1% TritonX-100 supplemented with Roche protease inhibitor cocktail). Lysates were centrifuged (5,000x g ...
-
bioRxiv - Cell Biology 2023Quote: ... Livers were collected on ice and incubated in digestion buffer (0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum) at room temperature ...
-
bioRxiv - Microbiology 2022Quote: ... The cell pellet was washed once with 50 mM Tris-HCl (pH 7.0) and resuspended in 1 mL of 50 mM Tris-HCl (pH 7.0) containing protease inhibitors (cOmplete, Roche Applied Science, Mannheim, Germany). The cells were disrupted with glass beads using a FastPrep system (Qbiogene ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were harvested and either lysed using sonication in HED buffer (25 mM HEPES, 10% glycerol, 1 mM DTT, and EDTA-free protease inhibitor (Roche, Basel, Switzerland)) containing 0.1 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Membranes were incubated overnight at 4°C in Odyssey blocking buffer containing 0.1% Tween-20 at 4°C and 1/1000 dilution of anti-c-myc mouse monoclonal antibody (Catalogue number 11 667 149 007, Roche, Bâle, Switzerland) or anti-β-tubulin mouse antibody (Catalogue number T9026 ...
-
bioRxiv - Immunology 2023Quote: ... and PLN from non-diabetic 6w– 8w female mice were minced and enzymatically dissociated with 1 mg/mL collagenase D (Roche Applied Science) and 25 U/mL DNase I in DMEM for 30 min at 37°C ...
-
Identification of plants functional counterparts of the metazoan Mediator of DNA Damage Checkpoint 1bioRxiv - Cell Biology 2023Quote: ... Samples were incubated with primary antibodies diluted 1:100 (rabbit pAb α-γH2A.X from SigmaAldrich, H5912 and mouse mAb α-GFP from Roche, 11814460001) in 1% BSA (in PBS ...
-
bioRxiv - Neuroscience 2023Quote: Lysates from cultured neurons were collected in NP40 lysis buffer (150 mM NaCl, 100 mM Tris-HCl, 1% NP40 in PBS) containing 1X protease inhibitors (cOmplete ULTRA tablets, Roche, cat# 05892970001). Samples were run on mini-protean TGX gels (Biorad ...
-
bioRxiv - Immunology 2023Quote: ... Cationic lipid N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl-sulfate (Dotap, Liposomal Transfection Reagent, Cat#1202375, Roche, Nonnenwald, Penzberg, Germany) was used for the conjugation of phosphorothioated miRNAs and Dotap-formulated miRNAs were used for in vitro delivery of miRNAs ...
-
bioRxiv - Molecular Biology 2023Quote: ... then treated with blocking reagent (Genostaff) for 15 min and with alkaline phosphatase-conjugated anti-DIG antibody (1:2,000; Roche Diagnostics, Basel, Switzerland) for 1 h at room temperature ...
-
bioRxiv - Cancer Biology 2023Quote: ... a maximum of 1 μg of sheared DNA was used for library preparation using the KAPA HTP Prep Kit (KAPA Biosystems, KK8234) following manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: Cell viability of cell lines in the presence of increasing concentrations of different drugs was assayed using the cell proliferation reagent WST-1 (Roche, Mannheim, Germany) after 72 h-treatments as previously reported 35 ...
-
bioRxiv - Developmental Biology 2023Quote: ... for 5 min or TRAP staining with a TRAP/ALP Stain Kit (FUJIFILM Wako Pure Chemical Corporation) for 30 min or ALP staining with NBT/BICP solution (Roche; 1:100) for 15 min followed by AR staining prepared from 1% AR Solution at pH 6.3-6.4 (MUTO PURE CHEMICALS CO. ...
-
bioRxiv - Molecular Biology 2024Quote: ... qPCR amplification of a 1:10 dilution of cDNA was performed in triplicate on a LightCycler® 480 II thermal cycler (Roche, France) using the LightCycler® 480 SYBR Green I Master mix and specific primers (Table S2) ...
-
bioRxiv - Developmental Biology 2024Quote: ... the extirpated tongues from non- transgenic and Lgr5-EGFP-IRES-CreERT2;R26-mTmG reporter mice were injected with approximately 100-200 μl 1% Dispase II (neutral protease; Roche, Indianapolis, IN) dissolved in Balanced Salt Solution (BSS ...
-
bioRxiv - Microbiology 2024Quote: ... the RNA-seq directional libraries were generated for 1 μg rRNA-depleted RNA using the KAPA Stranded mRNA-Seq Kit (Kapa Biosystems, KK8421). The rRNA-depleted RNA was fragmented ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Probes were detected by incubation overnight at 4°C in a 1:5000 dilution of Anti-DIG-AP antibody (Roche, Catalog# 11093274910) in Blocking Buffer ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... dissected samples were homogenized with a pestle in 1% sodium dodecyl sulfate in PBS supplemented with a protease inhibitor cocktail (cOmpleteTM mini, EDTA free, Roche, Basel, Switzerland). The resultant lysate was centrifuged at 20,000× g for 15 min ...
-
bioRxiv - Developmental Biology 2024Quote: ... then treated with blocking reagent (Genostaff) for 15 min and alkaline phosphatase-conjugated anti-DIG antibody (1:2,000; Roche Diagnostics, Basel, Switzerland) for 1 h at room temperature ...
-
bioRxiv - Pathology 2024Quote: ... Kidney fragments from each replicate was then re-suspended in a normalised volume of extraction buffer (10 mM Tris, 150 mM NaCl, 1% Triton X-100, 25 mM EDTA, Roche protease inhibitor) such that 1 mg kidney/µl solution ...
-
bioRxiv - Pathology 2024Quote: ... the aortic sections were incubated overnight at 4°C in a water-humidified chamber with anti-DIG-AP antibody diluted in blocking buffer (Roche, 1:500), followed by a three-time wash with TBST on the next day ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... The probe was detected by incubating samples overnight at 4°C with a 1:1500 dilution of Anti-DIG-POD antibody (Roche, Catalog# 11207733910) in Blocking Buffer ...
-
bioRxiv - Pathology 2024Quote: Multicellular BBB organoid arrays were formed for 48 h and consequently pre-incubated with zosuquidar (RO1033295-198-004, Roche Basel, 1 µM) in Endothelial Basal Medium (EBM-2 ...
-
bioRxiv - Developmental Biology 2024Quote: ... Digoxigenin- or fluorescein-labeled riboprobes were synthesized as described in previous work (58) and detected with anti-digoxigenin-POD or anti-fluorescein-POD antibodies (1:2000, Roche/Sigma-Aldrich) blocked with 10% heat-inactivated Horse serum and 10% Western Blotting Blocking Reagent (Roche) ...
-
bioRxiv - Immunology 2024Quote: ... diluted in 1 mL PBS and homogenized by bead-beating with 1 mm ceramic beads once for 40 s 6000 rpm using a MagNA Lyser (Roche Diagnostics GmbH). After 7 days of C ...
-
bioRxiv - Genomics 2024Quote: ... 0.5% N-Lauroylsarcosine) and resuspended in 100 μl of complete LB3 (LB3 containing 1 × Roche cOmplete Mini Protease Inhibitor Cocktail (Roche, Cat.N: 04693159001)) ...
-
bioRxiv - Genetics 2024Quote: Paired-end sequencing libraries for QTL-Seq analysis were prepared with >1 µg of DNA using a KAPA HyperPrep PCR-free kit (Roche, Basel, Switzerland) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... Total RNA was extracted from HCT116 and THP-1 cell lines using the Roche High Pure FFPE Extraction Kit (Roche Life Sciences) and amplified using the NuGen Ovation FFPE Amplification System v3 (NuGen San Carlos) ...
-
bioRxiv - Cancer Biology 2024Quote: ... and 1 tablet of protease and phosphatase inhibitors per 50 mL (cOmplete™ ULTRA Tablets Mini EDTA-free and PhosSTOP, Roche Diagnostics) and sonicated with Vibracell (10 cycles ...
-
bioRxiv - Cancer Biology 2024Quote: ... cells were washed in ice-cold 1X PBS before being scraped into 200 µL of ice-cold 1X PBS containing 0.1% NP-40 (1 tablet of the cOmplete Protease Inhibitor Cocktail (Roche, Cat No. 11697498001) and 1 tablet of the PhosSTOP Phosphatase Inhibitor Cocktail (Roche ...
-
bioRxiv - Cell Biology 2024Quote: ... 25 µg/ml digitonin and 1 M hexylene glycol) supplemented with protease inhibitor cocktail (cOmplete Mini EDTA-free tablets, Roche, Basel, Switzerland) for 10 min at 7°C rotating ...
-
bioRxiv - Bioengineering 2024Quote: ... Specific cell types within these sections were identified using a series of primary antibodies: 1:500 rabbit anti-ERG (Roche, Basel, Switzerland) for endothelial cells ...
-
bioRxiv - Cell Biology 2024Quote: ... and 1% Triton X-100) with either 1mM CaCl2 or 1mM EGTA supplemented with complete protease and phosphatase inhibitors (Roche, Mannheim, Germany). Lysates were subjected to two rounds of sonication for 10 s each ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... subsequently resuspended in 200µl of Lysis Buffer (1% SDS, 10 mM EDTA, 50 mM Tris-HCl pH 8, 1x cOmplete™, EDTA-free Protease Inhibitor Cocktail (Roche #04693132001)) and frozen with liquid nitrogen ...
-
bioRxiv - Developmental Biology 2024Quote: ... pH 8.0 50 mL / 5 M NaCl 30 mL / 5 g Sodium Deoxycholate / 1 g SDS / 1 L SDS g / NP-40 10 mL / MQ up to 1 L) containing 1x protease inhibitor (cOmplete™, EDTA-free Protease Inhibitor Cocktail [Roche, 16829900]). After centrifugation at 10,000 G ...
-
bioRxiv - Genomics 2020Quote: ... for 3 mins at 80°C in hybridization solution (10 mM Tris-HCl pH 7.2, 70% formamide, 0.5% Roche 11096176001 blocking reagent). Hybridization was carried out for 2 hours at RT in the dark ...
-
bioRxiv - Plant Biology 2020Quote: ... in a total volume of 6 μL containing 0.5 mM of each specific primer and 3 μl of SYBR Green I Master Mix (Roche Applied Science). The second derivative maximum method was used to determine Cp values and PCR efficiencies were determined using LinRegPCR software (http://LinRegPCR.nl) ...
-
bioRxiv - Cell Biology 2020Quote: ... and AdRb (5′-TCCTCGGCCG-3′) were ligated to the DpnI digested fragments in an overnight reaction at 16° C using T4 DNA ligase (Roche, 799009). After incubation the ligase was heat-inactivated at 65° C for 10 minutes ...
-
bioRxiv - Microbiology 2024Quote: ... and phoD-R1083 (5’-CTG SGC SAK SAC RTT CCA-3’) (Ragot et al., 2015) in 25 µL reactions with KAPA2G Robust HotStart PCR kit (KAPA Biosystems, Roche ...
-
bioRxiv - Microbiology 2024Quote: ... and phoD-R1083 (5’-CTG SGC SAK SAC RTT CCA-3’) (Ragot et al., 2015) in 25 µL reactions with KAPA2G Robust HotStart PCR kit (KAPA Biosystems, Roche; 0.5 U KAPA2G Robust HotStart DNA Polymerase ...
-
bioRxiv - Genomics 2024Quote: ... Samples were diluted in low-TE buffer and were subjected to 3 different miniaturized library prep methods: KAPA HyperPlus (Roche, KK8514), DNA Prep (Illumina ...
-
bioRxiv - Biochemistry 2023Quote: 106 cells were washed with 3 mL of cold PBS and resuspended in 50 µL solution containing 100 µg/mL neuraminidase from Clostridium perfringens (Roche, #11585886001). Cells were incubated for 1 h at 37 °C and washed twice with PBS before incubation with LiLA.
-
bioRxiv - Cancer Biology 2023Quote: ... along with a panel of 3 reference genes (PUM1, RPL13A, ACTB) was performed using TaqMan qPCR chemistry on the Light Cycler 480 II (Roche Diagnostics). According to previously established methods 7 ...