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Citations for Roche :
651 - 700 of 5547 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... All SYBR Green assays were completed with LightCycler® 480 SYBR Green I Master (Roche, Switzerland). Melt curve analysis was always included in SYBR Green amplifications.
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bioRxiv - Microbiology 2020Quote: ... The IHC assay was carried out on a Discovery ULTRA automated staining instrument (Roche Tissue Diagnostics) with a Discovery ChromoMap DAB (Ventana Medical Systems ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative PCR assays were performed in duplicate on the LightCycler 480 platform (Roche, Indianapolis, IN, USA).
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bioRxiv - Microbiology 2020Quote: ... The infectivity of viral stocks was determined using a colorimetric reverse transcriptase assay (Roche Life Sciences). VSV-G complemented NL4.3 R-E-viral stocks were generated as previously reported [38] and RT activity measured using a PCR based method [41].
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bioRxiv - Microbiology 2021Quote: ... The IHC assay was carried out on a Discovery ULTRA automated staining instrument (Roche Tissue Diagnostics) with a Discovery ChromoMap DAB (Ventana Medical Systems ...
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bioRxiv - Immunology 2021Quote: Real-time PCR assays for the detection of mRNAs were performed using Light Cycler System (Roche) and 384-Well Reaction Plates (Roche) ...
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bioRxiv - Cancer Biology 2020Quote: H1975 cells were lysed in RIPA lysis assay buffer (Pierce) supplemented with enzyme inhibitor cocktail (Roche). The samples were separated on a 4–20% SDS-polyacrylamide gel (Biorad) ...
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bioRxiv - Physiology 2022Quote: ... Primers were designed using Universal Probe Library Assay Design Center (Roche Applied Science, Burgess Hill, UK) or ...
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bioRxiv - Cell Biology 2022Quote: ... cells were lysed with the radioimmune precipitation assay buffer containing a cocktail of protease inhibitors (Roche). After removal of the cell debris ...
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bioRxiv - Molecular Biology 2023Quote: ... The real-time qPCR primers were designed using the universal probe library assay design center (Roche) and UCSC Genome Bioinformatics (table 3) ...
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bioRxiv - Genetics 2023Quote: ... or TaqMan gene expression assays (see details below) on the LightCycler 480 (Roche Diagnostics, Mannheim, Germany). Human tissue was obtained from the NIH NeuroBioBank’s Brain and Tissue repository at the University of Maryland ...
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bioRxiv - Biochemistry 2023Quote: ... Denaturation assays were performed in a 384-well plate in a qPCR machine (Roche Lightcycler II) using a temperature range of 25-99 °C ...
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bioRxiv - Microbiology 2024Quote: ... The IHC assay was carried out on a Discovery ULTRA automated-staining instrument (Roche Tissue Diagnostics) with a Discovery ChromoMap DAB kit (Ventana Medical Systems ...
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bioRxiv - Cancer Biology 2024Quote: ... 4 tetrazolium]-bis(4-methoxy-6-nitro)-benzene sulfonic acid hydrate (XTT)-based colorimetric assay (Roche) according to manufacturer-recommended protocols ...
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bioRxiv - Microbiology 2024Quote: ... The qPCR assay was prepared with Kapa Fast SYBR Green Master Mix (Kapa Biosystems, Cat. KK4824) and run on the QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Genetics 2024Quote: ... The assay was performed using 5.0 μL of FastStart Universal Probe Master (Rox; Roche, Basel, Switzerland), 0.2 μL of Custom TaqMan SNP Genotyping Assay (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... High Sensitivity DNA Analysis Kit and KAPA SYBR FAST qPCR Kit (Roche). WGS was performed on the HiSeq X (HCS HD 3.5.0.7 ...
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bioRxiv - Cancer Biology 2022Quote: ... RNase free kit (Roche). RNA amount was quantified and cDNA was prepared using TaqMan Reverse Transcription Reagents (Applied Biosystems) ...
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bioRxiv - Cell Biology 2020Quote: ... the membranes were hybridized overnight at 40°C with digoxigenin (DIG)-labeled DNA probes in DIG Easy Hyb solution (Roche). After low and high stringency washes ...
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bioRxiv - Microbiology 2020Quote: ... Membranes was incubated secondary antibody for an hour in 5% milk and developed using Lumi-light western blot substrate (Roche) to detect HRP ...
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bioRxiv - Cell Biology 2022Quote: ... From each cell lysate 10 µg of protein were loaded to an SDS-Page (10 % self-made gel) and then blotted to a PVDF western blot membrane (Ref: 3010040001, Roche). The membrane was incubated with rabbit monoclonal antibody against total ERK (Ref ...
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bioRxiv - Molecular Biology 2022Quote: ... was run on a 15% polyacrylamide gel containing 7M urea and 10 µg/ml APM and then transferred to positively charged nylon membranes (Roche). Pre-hybridization and hybridization steps were performed with Dig Easy Hyb (Roche) ...
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bioRxiv - Plant Biology 2022Quote: GFP was detected on the membranes using anti-GFP mouse-IgG monoclonal (clones 7.1 and 13.1) antibody (Roche, Basel, Switzerland) in a 1/500 dilution in 5% (w/v ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked in TBST with 5% milk protein and probed with the following antibodies: 1:1000 anti-HA (Roche), then 1:1500 goat anti-rat IgG-HRP (Dako) ...
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bioRxiv - Microbiology 2021Quote: ... release into growth medium (as direct indicator of cell membrane integrity loss) was performed with the Cytotoxicity Detection KitPLUS (Roche) according to the supplier’s manual.
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CRISPR-Csx28 forms a Cas13b-activated membrane pore required for robust CRISPR-Cas adaptive immunitybioRxiv - Biochemistry 2021Quote: ... The membrane fraction was resuspended in membrane resuspension buffer (50 mM sodium phosphate buffer, 300 mM NaCl, 5% glycerol, EDTA-free protease inhibitor (Roche), 2% DDM ...
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bioRxiv - Developmental Biology 2021Quote: ... An equal amount of each protein sample was electrophoresed on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene difluoride (PVDF) membranes (3010040001; Roche). The membrane was blocked with 5% non-fat dry milk for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... and proteins were transferred to an activated (100% ethanol, 1 min; followed by two washing steps with water) PVDF membrane (Roche Diagnostics GmbH ...
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bioRxiv - Microbiology 2022Quote: ... Nucleic acids were spotted (for dot-blot) or electro transferred (for northern-blot) to positively-charged nylon membranes (Roche Diagnostics), and hybridized with DIG-labeled full-length riboprobes as previously described [8 ...
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bioRxiv - Microbiology 2022Quote: ... Nucleic acids were spotted (for dot blotting) or electro-transferred (for Northern blot) to positively-charged nylon membranes (Roche Diagnostics), and hybridized with DIG-labeled riboprobes as previously described (22).
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bioRxiv - Biochemistry 2024Quote: ... the proteins were transferred to a PVDF membrane and immunodetected with an anti-Myc-HRP antibody (Myc-HRP, 1:5000, Roche). The remaining 10% of the immunoprecipitated samples together with 50 μg of the input were subjected to the same procedure but probed with an anti-GFP antibody (JL-8 ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was then washed and incubated 1 hs at RT with goat anti-mouse secondary antibody conjugated to HRP (Roche) at 1/20000 dilution in PBST - 5% milk.
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bioRxiv - Plant Biology 2023Quote: ... membranes were blocked with 5% skim milk and then incubated in the primary anti-GFP mouse monoclonal antibody (Roche, 11814460001), followed by goat anti-mouse HRP conjugate (BioRad ...
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bioRxiv - Microbiology 2024Quote: ... The membranes were blocked for 2 hours at room temperature in 1% blocking solution (1% Western Blocking Reagent (WESTBL-RO, Roche) in TBS ...
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bioRxiv - Microbiology 2024Quote: ... T the gels were electroblotted on positively charged nylon membranes (Amersham Hybond-N RPN 203 N) followed by UV crosslinking and hybridised in DIG EasyHyb buffer (Roche) at 68 °C with gentle agitation overnight ...
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bioRxiv - Microbiology 2024Quote: ... The heat-denatured RNAs were separated on a 1% agarose gel (95% 1× MOPS buffer and 5% formaldehyde) and transferred to a positively-charged nylon membrane (Roche) by capillary flow ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The presence of proteins in the input was determined by SDS-PAGE/western blot and probing the membranes with anti-GFP (11814460001/54732800, Roche) or anti-RFP (AB_2631395 ...
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bioRxiv - Cell Biology 2020Quote: Mammalian cells were lysed in Radioimmunoprecipitation assay buffer (RIPA) buffer with the addition of protease (Roche cOmplete), and phosphatase inhibitors (GB Sciences) ...
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bioRxiv - Immunology 2021Quote: Macrophage cell lysates were lysed in radioimmunoprecipitation assay (RIPA) buffer containing protease inhibitor cocktails and PhosSTOP (Roche). Protein concentration was measured using the BCA Protein Assay Kit (Pierce) ...
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bioRxiv - Immunology 2021Quote: ... The primers were designed using the Roche Universal Probe Library (UPL) Assay Design tool (Roche, Basel, Switzerland) with the indicated UPL probes ...
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bioRxiv - Immunology 2022Quote: Plasma viral loads were assessed using Amplicor HIV-1 Monitor Ultrasensitive Assay (Roche Molecular System, Branchburg, NJ).
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bioRxiv - Cell Biology 2020Quote: ... and LacZ activity was measured using a liquid β-galactosidase assay employing chlorophenolred-ß-D-galactopyranoside (Roche) as a substrate.
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bioRxiv - Cancer Biology 2021Quote: ... cell viability was evaluated using a colometric formazan-based cell viability assay (WST-1; Roche, Sigma-Aldrich).
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bioRxiv - Neuroscience 2022Quote: ... The reverse transcriptase activity of the viruses was measured by using the colorimetric Reverse Transcriptase Assay (Roche).
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bioRxiv - Microbiology 2020Quote: A colorimetric assay based on the measurement of LDH activity released by damaged cells was used (Roche). Experiments were performed in 96-well plate and the manufacturer’s instructions were followed ...
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bioRxiv - Plant Biology 2020Quote: ... Gene primers and probes were designed using Universal Probe Library (UPL) assay design centre (Roche Diagnostics Ltd.). RT-qPCR was performed using FastStart Universal Probe Master Mix (Roche ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cell viability was measured by trypan blue exclusion or WST-1 assays (Roche Diagnostics GmbH, Mannheim, Germany).
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bioRxiv - Physiology 2020Quote: ... The plasma was separated and stored at −80°C for insulin assay according to manufacturer’s instructions (Roche).
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bioRxiv - Microbiology 2020Quote: ... The immunohistochemistry (IHC) assay was carried out on a Discovery ULTRA automated staining instrument (Roche Tissue Diagnostics) with a Discovery ChromoMap DAB (Ventana Medical Systems ...
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bioRxiv - Genetics 2021Quote: ... which was performed with LightCycler 480 SYBR Green І Master Gene Expression Assays (Roche Molecular BiocheMHcals, Germany), is quantitated for the expression of β-actin/Akt/mTOR/p70S6K/eIF4E by employing the 2−ΔΔCT value models [31] ...