Labshake search
Citations for Roche :
601 - 650 of 5547 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2020Quote: ... Then WST-1 assay was performed by adding WST-1 reagent (cell proliferation reagent, Roche) directly to the culture wells ...
-
bioRxiv - Immunology 2021Quote: ... and the enzymatic activity was evaluated by chemiluminescence (β-Gal reporter gene assay, Roche Diagnostics). Anti-CD4 neutralizing mAb L3T4 was used as a control for the blockage of cell fusion (5 µg/mL was preincubated in HeLa-P5 cells for 30 min at 37°C before co-culture with Env+-HeLa cells).
-
bioRxiv - Plant Biology 2020Quote: ... The HRM assay was performed with LightCycler® 480 System II (Roche Life Science, Germany). The HRM data was analyzed using the Melt Curve Genotyping and Gene Scanning Software (Roche Life Science ...
-
STARCH SYNTHASE 4 is required for normal starch granule initiation in amyloplasts of wheat endospermbioRxiv - Plant Biology 2021Quote: ... Glucose was measured in the supernatant using the hexokinase/glucose-6-phosphate dehydrogenase assay (Roche), for calculation of starch content in glucose equivalents ...
-
bioRxiv - Cell Biology 2022Quote: ... Intron-spanning primers were designed using the Universal Probe Library (UPL) Assay design centre (Roche). Primer sequences and UPL probes were total SNCA F-tgggcaagaatgaagaaggagc ...
-
bioRxiv - Cancer Biology 2022Quote: Cells were lysed in radioimmunoprecipitation assay (RIPA) buffer supplemented with a cocktail of protease (Roche) and phosphatase inhibitors (Sigma-Aldrich) ...
-
bioRxiv - Genomics 2020Quote: ... SYBR Green assay was performed on Light Cycler 480 instrument (2x Probe Master Mix, Roche). All primer sequences are listed in Table S11 ...
-
bioRxiv - Microbiology 2022Quote: ... (2) the quantitative Roche Spike Elecsys Anti-SARS-Cov-2 S assay (Roche, IND, USA), which measures spike total antibody concentrations ...
-
bioRxiv - Physiology 2020Quote: Apoptosis was determined by terminal deoxynucleotidyl nick-end labeling (TUNEL) per assay manufacturer’s protocol (Roche Diagnostics ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were lysed in radioimmunoprecipitation assay (RIPA) buffer containing cOmplete EDTA-free protease inhibitor (Roche) and 10U/ml benzonase nuclease (Santa Cruz) ...
-
bioRxiv - Immunology 2021Quote: ... RT-qPCR assays were performed with SYBR FAST qPCR Master Mix (Kapa Biosystems, Cat# KK4602), using primers indicated in Supplementary Table 2 ...
-
bioRxiv - Immunology 2023Quote: Anti-nucleocapsid IgG was measured using the Elecsys Anti-SARS-COV-2 assay (Roche; 09203095190) run on a Cobas e411 analyser (Roche ...
-
bioRxiv - Neuroscience 2023Quote: ... Gene expression was determined using assays designed with the Universal Probe Library from Roche (www.universalprobelibrary.com). For each qPCR assay ...
-
bioRxiv - Plant Biology 2023Quote: ... and the released glucose was assayed using the hexokinase/glucose-6-phosphate dehydrogenase assay (Roche). For mature grains ...
-
bioRxiv - Genetics 2023Quote: ... The RT-qPCR assay was carried out in a LightCycler480 Real Time PCR system (Roche) using the TaqMan gene expression assay ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... All assays were carried out in triplicate on the Roche Lightcycler 96 platform (Roche, Switzerland) with four quantification standards included.
-
bioRxiv - Neuroscience 2023Quote: ... The free fatty acids (FFA) levels were determined using a colorimetric assay (Roche, Mannheim, Germany). Cholesterol was determined by colorimetric assay (Erba Lachema ...
-
bioRxiv - Biochemistry 2022Quote: The thermal shift assay was performed on a LightCycler 480 Real-Time PCR System (Roche) in 96-well white plates (Armadillo plate ...
-
bioRxiv - Genetics 2023Quote: ... a Bromodeoxyuridine (BrdU) based proliferation assay was conducted according to the manufacturer’s introductions (Roche, # 11647229001). The incorporation of BrdU was detected calorimetrically after six to eight hours of incubation time at a wavelength of 370 nm and normalized to cells devoid of BrdU ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were lysed in radioimmunoprecipitation assay buffer containing complete protease inhibitor cocktail (Roche, Basel, Switzerland) on ice and ultracentrifuged ...
-
bioRxiv - Physiology 2024Quote: ... The cell proliferation assay was performed according to the manufacturer’s instructions (Roche Applied Sciences, 11647229001).
-
bioRxiv - Genetics 2020Quote: ... PCR KIT (Roche). For each reaction ...
-
bioRxiv - Molecular Biology 2024Quote: ... followed by the detection Kit ChromoMap DAB kit (760-4304, Roche). Then ...
-
bioRxiv - Cell Biology 2020Quote: ... Equal amounts of protein were separated on 10%-15% SDS-PAGE gels and transferred to PVDF membranes (Roche, 03010040001). The membranes were probed with the indicated primary antibodies followed by the appropriate HRP-conjugated secondary antibodies ...
-
bioRxiv - Microbiology 2022Quote: ... The pellet (crude membrane preparation) was resuspended in 20mM Tris pH 8.0 plus protease inhibitor cocktail (Roche EDTA free), flash-frozen in liquid N2 and kept at -80°C.
-
bioRxiv - Microbiology 2020Quote: ... The membrane was incubated with horseradish peroxidase (HRP)-conjugated rat anti-HA (clone 3F10) monoclonal antibodies (Roche, Indianapolis, IN) at a dilution of 1:5,000 for 2 hours ...
-
bioRxiv - Cell Biology 2020Quote: ... Membranes were immunoblotted for 1 hr at 22°C using the following antibodies: rat anti-HA (1:3000; Roche), mouse anti-V5 (1:5000 ...
-
bioRxiv - Cell Biology 2021Quote: ... transferred to PVDF membranes (Amersham Hybond) and probed with following antibodies: mouse anti-HA-HRP (1:1000) (12013819001, Roche) rabbit anti-EMC6 (1:300 ...
-
bioRxiv - Molecular Biology 2022Quote: ... The gel was briefly rinsed with 1 x TBE and RNA was transferred to positively charged Nylon membrane (Roche) using Fastblot (Biometra ...
-
bioRxiv - Cell Biology 2022Quote: ... The protein lysate was electrophoresed into 6-10% SDS-PAGE gels and transferred to a PVDF membrane (Roche, 03010040001). After blocking with 5% BSA ...
-
bioRxiv - Microbiology 2020Quote: ... separated by electrophoresis in a 0.8% agarose gel and transferred onto a nylon membrane (Hybond N+, Roche Molecular Biochemicals). The membrane was hybridized with digoxigenin-labeled DNA probes synthesized with a PCR DIG probe synthesis kit (Roche Molecular Biochemicals ...
-
bioRxiv - Immunology 2020Quote: ... the pellet was resuspended in 50 mL membrane wash buffer supplemented with 1X protease inhibitor (Roche cat no. 04693159001) and 10mg/mL lysozyme and let shake on ice for 30 minutes before sonication at 70% amplitude ...
-
Toxoplasma GRA15 limits parasite growth in IFNγ-activated fibroblasts through TRAF ubiquitin ligasesbioRxiv - Immunology 2020Quote: ... The membrane was blotted overnight at 4°C with rat antibody against the HA tag (Roche, 1/500 dilution), TRAF2 and TRAF6 rabbit antibodies (Suppl ...
-
bioRxiv - Developmental Biology 2022Quote: ... Transfer membranes were then incubated with a 1:10,000 dilution of primary mouse anti-6xHis antibody (Roche, cat. #11922416001) for 1 h ...
-
bioRxiv - Microbiology 2021Quote: ... The membrane was incubated with gentle rocking in DIG Easy Hyb solution (Roche Cat. No. 11 796 895 001) at the respective probe hybridisation temperature (Thyb ...
-
bioRxiv - Cell Biology 2023Quote: ... the membranes were blocked using non-fat dry milk and stained with an anti-GFP primary (Roche; 1:1000) and a goat anti-mouse secondary antibody coupled to horseradish peroxidase (HRP ...
-
bioRxiv - Molecular Biology 2022Quote: ... The membrane was transferred to a blocking buffer (1 M maleic acid solution pH7.4, 1% nucleotide blocking reagent (Roche)) for 30 minutes and then incubated in antibody-solution (anti-dioxigenin-AP fragments in blocking buffer) ...
-
bioRxiv - Biochemistry 2023Quote: ... the membrane was incubated with 1 nM DIG (Digoxigenin)-labeled Telomere probes in Hybridization solution (DIG Easy Hyb, Roche) at 42°C ...
-
bioRxiv - Pathology 2023Quote: ... Approximately 30-50 μg of proteins were separated by 10% SDS-PAGE and transferred onto a PVDF membrane (Roche). The membrane was blocked with 5% non-fat milk in PBS for 1 h at room temperature and then incubated with the specific primary antibodies at 4°C overnight ...
-
bioRxiv - Developmental Biology 2022Quote: ... The membranes were incubated in a blocking solution containing maleic acid buffer (pH 7.5) and 1 % blocking reagent (Roche). Subsequently ...
-
bioRxiv - Neuroscience 2022Quote: ... the membranes were incubated overnight at 4° C on a rotator with primary antibody in Western Blocking Reagent (Roche), if not stated otherwise ...
-
bioRxiv - Molecular Biology 2023Quote: ... membranes were incubated overnight at 4°C with monoclonal mouse anti-GFP antibody (Roche; Basel, CH; dilution 1:500) or monoclonal rat anti-HA antibody (Roche ...
-
bioRxiv - Plant Biology 2024Quote: ... the membranes were incubated with primary antibody for 2.5 h (GFP, Abcam, Cambridge, UK: rabbit, 1:10000; HA (Roche), rat ...
-
bioRxiv - Molecular Biology 2021Quote: ... The real-time qPCR primers were designed using the universal probe library assay design center (Roche) and UCSC Genome browser (Table 2) ...
-
bioRxiv - Immunology 2022Quote: Full-length HIV clones were quantified by reverse transcriptase (RT) enzyme-linked immunosorbent assay (ELISA) (Roche) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... PTPN2 mRNA expression was assessed using a Taqman SNP genotyping assay and on a LightCycler (Roche).
-
bioRxiv - Plant Biology 2021Quote: ... collected and the enzymatic assay was performed using red-β-D-galactopyranoside (CPRG, Roche Applied Science) as substrate as described (Yeast Protocols Handbook ...
-
bioRxiv - Cell Biology 2022Quote: ... Gene expression was determined using assays designed with the Universal Probe Library (UPL) from Roche (www.universalprobelibrary.com). For each qPCR assay ...
-
bioRxiv - Cell Biology 2022Quote: ... The real-time qPCR primers were designed using the universal probe library assay design center (Roche). All samples were amplified in triplicates in a 384-well plate using the following cycling conditions ...
-
bioRxiv - Plant Biology 2021Quote: ... Quantitative real-time PCR (RT-qPCR) assay was performed using FastStart Essential DNA Green Master (Roche) on a CFX Connect™ Real-Time System (Bio-Rad) ...