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Citations for Roche :
901 - 950 of 5547 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... They were then lysed in radio-immunoprecipitation assay (RIPA) buffer supplemented with PhosSTOP phosphatase and CompleteMini protease inhibitors (Roche, Switzerland) for protein extraction ...
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bioRxiv - Plant Biology 2023Quote: ... Primers and Taqman probes were designed using Probe Finder software in the Universal Probe Library (UPL) Assay Design Center (Roche). All PCR reactions were performed using a StepOne System (Applied Biosystems) ...
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bioRxiv - Microbiology 2023Quote: Poly dA/oligo dT substrate for Pol-Prim stimulation assays was created by mixing 100 nanomoles of poly [dA] (average length 250-500nt) (Roche) with 5 nanomoles of oligo [dT] (average length 18nt ...
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bioRxiv - Cancer Biology 2023Quote: ... Hybridization of the DIG-telomere probe and chemiluminescent detection was performed according to the protocol from the TeloTAGGG Telomere Length Assay (Roche). Briefly ...
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bioRxiv - Plant Biology 2023Quote: ... Quality and quantity of sequencing libraries were evaluated and quantified using Bioanalyzer and KAPA Library Quantification assay (Roche, Basel Switzerland). Paired-end sequencing (2 × 75 bp ...
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bioRxiv - Genetics 2024Quote: Total protein was isolated from ∼15 larval brains or adult fly heads by homogenization of fly tissue in Radioimmunoprecipitation Assay (RIPA) buffer containing protease inhibitors (Roche). Approximately 20-30µg of total protein was separated using 4-20% SDS-PAGE (Biorad) ...
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bioRxiv - Bioengineering 2024Quote: ... Total cell lysates were extracted from CAR T cells using radioimmunoprecipitation assay (RIPA) buffer (Thermo) containing protease inhibitor cocktail (Roche). Total protein concentration was determined using Pierce BCA assay (Thermo) ...
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bioRxiv - Immunology 2024Quote: Proteins were extracted from tissues or cells in radioimmunoprecipitation assay (RIPA) buffer (Beyotime) containing a complete protease inhibitor cocktail (Roche), resolved by SDSPAGE ...
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bioRxiv - Physiology 2024Quote: ... the legs of each genotype were homogenized in Radioimmunoprecipitation assay buffer containing a complete Protease Inhibitor Cocktail (Roche, Basel, Switzerland). A Filter Aided Sample Preparation (FASP ...
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bioRxiv - Bioengineering 2024Quote: ... miRNA amplification and expression were performed with TaqMan miRNA assay (Thermo) as instructed and measured on LightCycler® 96 (Roche).
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bioRxiv - Cancer Biology 2021Quote: ... 30-diaminobenzidine tetrahydrochloride (DAB) or Purple Kit (Chromo Map DAB or Purple Kit, Ventana, Roche; DAB (Dako) and nuclei were counterstained with Carazzi’s hematoxylin ...
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bioRxiv - Cancer Biology 2021Quote: ... The exome captured by the SeqCap wash kit and SeqCap pure capture bead kit (both from Roche). The Illumina Novaseq platform was used to sequence 150 bases (paired-end sequencing ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was isolated using a commercial extraction kit (High Pure PCR template preparation kit; Roche Diagnostics). Indexed paired-end libraries were preparedwith the Nextera XT DNA library preparation kit (Illumina Inc ...
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SARS-CoV-2 Point Mutation and Deletion Spectra, and Their Association with Different Disease OutcomebioRxiv - Microbiology 2022Quote: ... Purified DNA pools were further processed using the DNA library preparation kit Kapa Hyper Prep kit (Roche), during which each pool was indexed using SeqCap Adapter Kit A/B (Nimblegen ...
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bioRxiv - Plant Biology 2022Quote: ... The EMSA was carried out utilizing the manufacturer’s instructions (DIG-gel shift kit 2nd generation kit, Roche).
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bioRxiv - Biochemistry 2024Quote: ... The amount of acetic acid released was determined using an F-kit acetate kit (Roche, Basel, Switzerland). The synergistic effect between AoAXEB and TlXyn (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... Colorimetric in situ hybridization assays used sense and anti-sense RNA probes labeled with DIG RNA labeling mix (Roche, Basel, Switzerland), following a published procedure (Sharma et al. ...
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bioRxiv - Immunology 2020Quote: ... The proliferation rate of lymphocytes was measured using 5-Bromo-2-deoxyuridine (BrDU) assay as per the manufacturer instructions (Cell Proliferation ELISA BrDU, Roche, USA).
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bioRxiv - Biochemistry 2020Quote: A fluorescence-based thermal stability assay (differential scanning fluorimetry, DSF) (51) was performed using a LightCycler®480 II instrument (Roche). 20-μl samples were analyzed in 20 mM HEPES ...
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bioRxiv - Molecular Biology 2020Quote: ... were added and after an additional 6 hours BrdU was added and a cell proliferation assay was performed according to the manufacturer’s instructions (Roche Applied Sciences).
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bioRxiv - Cell Biology 2021Quote: Transduced MEFs were seeded in triplicate in a 96 well flat bottom tissue culture plate and WST-1 assay (Roche, Switzerland) performed as per manufacturer’s protocol at day 0 ...
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bioRxiv - Plant Biology 2021Quote: ... RT-qPCR primers (Table S1 and Table S2) were designed using the Universal Probe Library Assay Design Centre (Roche Life Sciences) and primer efficiency was determined using pooled cDNA samples ...
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bioRxiv - Microbiology 2020Quote: ... and subjected to a protease protection assay by resuspension in 250 μl of freshly prepared 1 mg/mL Pronase (Roche, 10165921001)/0.01% Saponin/PBS at 12°C for 1h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Total proteins were extracted from RAW264 using radioimmunoprecipitation assay (RIPA) buffer containing protease inhibitors (Complete Mini, Roche Diagnostics GmbH, Mannheim, Germany) and phosphatase inhibitors (PhosSTOP EASYPack ...
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bioRxiv - Cancer Biology 2021Quote: The terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was used for detecting cell apoptosis according to the manufacturer’s instructions (Roche Diagnostics, USA). In brief ...
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bioRxiv - Immunology 2020Quote: ... The specific primers for each target (listed below) and probes were designed by Universal ProbeLibrary Assay Design Center (Roche Applied Science). Primers used in this study were 5′-CCCTCTCTTTATCAACAAACTTGC-3′ and 5′-TTGTTTTCATGTTGGACCAGA-3′ for IFN-α ...
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bioRxiv - Microbiology 2022Quote: ... with the IDT E Assay First Line Screening primers and probe (Cat # 10006804) on a LightCycler 96 System (Roche, Cat# 05815916001).
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bioRxiv - Immunology 2024Quote: ... Sera were analysed for antibody directed against the SARS-CoV-2 Spike protein receptor-binding domain (S-RBD) and nucleocapsid (N) using an established automated electrochemiluminescence assay (Elecsys Anti-SARS-CoV-2 S and N, Roche diagnostics) [54] ...
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bioRxiv - Neuroscience 2024Quote: Weighed brain tissue samples were processed for protein assays by addition of 10X volume NP-40 buffer with added cOmplete Protease Inhibitor (Roche #04693132001) and PhosStop (Roche 04906837001 ...
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bioRxiv - Neuroscience 2023Quote: Weighed frozen tissue samples were processed for biochemical assays by adding 5X or 10X volume of chilled 1% NP40 + PBS homogenization buffer with added cOmplete Protease Inhibitor (Roche #04693132001) and PhosStop (Roche 04906837001 ...
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bioRxiv - Cancer Biology 2023Quote: ... and the protein lysates were prepared in radioimmunoprecipitation assay buffer supplemented with a protease inhibitor mixture (Roche Applied Science, IN, USA) and 2mM phenylmethylsulfonyl fluoride (Sigma-Aldrich) ...
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bioRxiv - Genetics 2023Quote: Whole-worm protein extracts were generated by sonication in radioimmunoprecipitation assay (RIPA) lysis buffer with cOmpleteTM Protease Inhibitor Cocktail (Roche #4693116001) and β-glycerophosphate (Sigma-Aldrich G6251) ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed and lysed in radioimmunoprecipitation assay buffer (RIPA) buffer supplemented with Complete® protease inhibitor cocktail (Roche, Basel, Switzerland) and Phosphatase Inhibitor Cocktail II (Abcam ...
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bioRxiv - Systems Biology 2024Quote: Troponin T analysis was performed by the Clinical Chemistry Departments at Skånes University Hospital Malmö on lithium heparin plasma using the Elecsys troponin T hs assay (Roche Diagnostics, Germany) run on a Cobas 6000 analyzer (Roche Diagnostics ...
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bioRxiv - Cell Biology 2024Quote: Samples were lysed in ice cold radioimmunoprecipitation assay (RIPA) buffer containing 1:10 PhosSTOP™ (#4906845001, Roche Diagnostics, Indianapolis, IN, USA) and 1:20 cOmplete™ ULTRA Protease Inhibitor Cocktail (#06538304001 ...
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bioRxiv - Molecular Biology 2024Quote: Remaining isolated mitochondria from our calcium and respiratory assays were pelleted and lysed in RIPA buffer supplemented with phosphatase inhibitors (Roche, 4906837001) and protease inhibitors (Sigma ...
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bioRxiv - Physiology 2024Quote: ... myotubes were washed using PBS then harvested by adding 50 μl Radioimmunoprecipitation Assay (RIPA) buffer containing protease inhibitors (Roche, Basel, Switzerland). Myotubes where scraped from the well and lysates centrifuged at 12000g for 5 min and supernatant collected ...
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bioRxiv - Bioengineering 2024Quote: ... perfusate from the device was collected from the outlet access ports and analyzed by the Cytotoxicity Detection KitPLUS assay (LDH, Roche, 04744926001) using the manufacturer-provided protocol ...
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bioRxiv - Neuroscience 2024Quote: Tissue extracts were obtained by lysing E16.0 mouse cortical samples in radioimmunoprecipitation assay (RIPA) buffer supplemented with a protease inhibitor solution (cOmplete, EDTA-free Protease Inhibitor Cocktail; Roche, #11873580001) and 100mM PMSF at 4°C for 2 hours ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were rinsed with ice-cold PBS and lysed with radioimmunoprecipitation buffer assay (RIPA) containing protease and phosphatase inhibitor cocktail (Roche; #05892791001) and incubation on ice for 10 min ...
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bioRxiv - Genetics 2024Quote: ... The IPC qPCR assay was performed in duplicate using 10 μl of 2x SYBR Green I Master (Roche Diagnostics, Mannheim, Germany), 0.2 μl each MSR_129 primer ...
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bioRxiv - Bioengineering 2021Quote: ... Microcarriers were subjected to total RNA extraction using a commercially available kit (High Pure kit, Roche, Indianapolis, IN). Total RNA was then used to make complementary DNA (cDNA ...
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bioRxiv - Cancer Biology 2021Quote: ... The detection kit for the antibodies is the UltraView DAB detection Kit (Ventana Medical Systems Inc./ Roche Diagnostic). A counter-staining of the nuclei was used for 12 minutes by Hematoxylin ...
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bioRxiv - Cell Biology 2021Quote: ... RNA sequencing libraries were prepared using a stand specific kit RNA-seq kit with ribosome depletion (KAPA Biosystems) and NEBNext Adapters and Multiplex Oligos for Illumina ...
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bioRxiv - Physiology 2024Quote: ... TUNEL staining was performed using a commercial kit (in situ Cell Death Detection Kit, TMR red – Roche, Switzerland). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... using Kapa SYBR Fast Universal Kit (Roche) with an annealing temperature of 60°C and with primers listed in Supplementary Table S1 ...
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bioRxiv - Developmental Biology 2021Quote: ... using Library Quantification Kit (KK4854, Kapa Biosystems), and multiplexed by pooling equimolar amounts of each library prior to sequencing ...
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bioRxiv - Plant Biology 2022Quote: ... using SYBR Fast Universal kit (Kapa Biosystems). The average threshold cycle (Ct ...
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bioRxiv - Molecular Biology 2020Quote: ... or Kapa Library Quantification kit (Kapa Biosystems). One hundred cycle paired end sequencing was performed on an Illumina HiSeq 4000 (single copy strains ...
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bioRxiv - Pathology 2020Quote: ... DAB Map detection kit (Roche Diagnostics KK) was used for visualization ...