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Citations for Roche :
701 - 750 of 5547 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: Protein was extracted from cells using Radioimmunoprecipitation assay (RIPA) lysis buffer containing 1X cOmplete Protease InhibitorCocktail (Roche) and 1X Phenylmethylsulfonyl fluoride (Sigma Aldrich ...
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bioRxiv - Genomics 2020Quote: ... This final probe set was used for the preparation of a SeqCap EZ Developer assay by Roche NimbleGen Technologies ...
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The loss-of-function of SOCS2 increases the inflammatory response to Staphylococcus aureus infectionbioRxiv - Immunology 2023Quote: ... Total protein was extracted with radio immune precipitation assay (RIPA) buffer containing phenylmethylsulphonyl fluoride (PMSF) (Roche, Switzerland) and a protease inhibitor cocktail (Roche ...
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bioRxiv - Molecular Biology 2022Quote: Radioimmunoprecipitation Assay (RIPA) buffer and cOmplete mini-proteinase inhibitors (1 tablet per 10 ml extraction solution, Roche) was used to homogenize striatal tissue and cells ...
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bioRxiv - Molecular Biology 2022Quote: Cell lysates were prepared in radioimmunoprecipitation assay buffer supplemented with protease inhibitor cocktail tablets (Roche, Basel, Switzerland), and total protein was measured using a Bicinchoninic Acid Protein Assay Kit (Beyotime Biotechnology ...
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bioRxiv - Cell Biology 2022Quote: ... For LC-MS/MS based experiments the kinase assay buffer contained 80 μM cold ATP (10519979001, Roche)) ...
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bioRxiv - Genetics 2022Quote: ... and the barcoded sequencing libraries were quantified using a standard curve-based qPCR assay (KAPA Biosystems, USA) and Agilent Bioanalyzer 2100 (Agilent ...
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bioRxiv - Cell Biology 2024Quote: ... The final pooled library was quantified using a KAPA qRT-PCR Library Quantification assay (Kapa Biosystems, 07960140001). RNA-seq libraries were sequenced on a NextSeq 2000 platform (Illumina ...
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bioRxiv - Genetics 2024Quote: ... TaqMan real-time polymerase chain reaction assays (PCR) consisted in 2.5 ul of Fast Master Mix (Roche), 0.125 ul of assay ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Roche, #11465007001) and CellTiter-Glo Luminescent Cell Viability (CTG ...
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bioRxiv - Cell Biology 2024Quote: ... protein lysates were prepared in a radioimmunoprecipitation assay (RIPA) buffer supplemented with a complete protease inhibitor (Roche). Proteins were separated by 6–15% SDS-PAGE ...
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bioRxiv - Microbiology 2023Quote: ... followed by library preparation with KAPA RNA HyperPrep kit (Roche, kit code KK8541), according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples (containing 20 μg of total protein) were mixed with loading buffer and separated on 12 % SDS-PAGE gels and transferred to PVDF membranes (Roche, Switzerland). After blocking with Tris-buffered saline with 0.1% Tween 20 and 5 % nonfat dry milk ...
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bioRxiv - Immunology 2021Quote: ... membranes were washed three times in TBS-T followed by blocking in 10% (v/v) western blotting reagent (Roche, Basel, Switzerland) for 1–2 h at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein lysate of 10-20 μg was run on 10–15% SDS-PAGE gel and transferred toa PVDF membrane (Roche, USA). The membrane was incubated for 60 min at room temperature in 5% BSA solution ...
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bioRxiv - Plant Biology 2021Quote: ... 150mM NaCl] supplemented with 1% Tween® 20 for 30 min before probing the membrane with either rat monoclonal anti-HA antibody (3F10, Roche) or mouse monoclonal ANTI-FLAG® antibody conjugated to HRP (M2 ...
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bioRxiv - Plant Biology 2020Quote: ... transferred to a PVDF membrane and immunoblotted with rat monoclonal anti-HA (High Affinity, clone 3F10, Roche, www.roche.com; 1:2000 dilution) and hybridized with peroxidase conjugated goat anti-rat (Polyclonal ...
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bioRxiv - Plant Biology 2021Quote: ... The digested DNA was separated in a 1 % agarose gel at 50 Volts for 72 hours at 4°C and transferred to a nylon membrane (Roche®) overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... The membrane was washed with Tris-buffered saline Tween and the signal visualized using the Supersignal ECL reagent (Roche, Indianapolis, IN). Quantification of western blot signal was performed by using ImageJ.
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bioRxiv - Cell Biology 2020Quote: ... membranes were incubated in 1% Triton X-100 for 30 min on ice followed by treatment with proteinase K (Roche 3115879001) at the indicated concentrations at 37°C for 5 min ...
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bioRxiv - Molecular Biology 2022Quote: ... The fragments were separated on 0.7% agarose gels buffered with 40 mM Tris-acetate at 4V/cm for 6 h and transferred overnight to nylon membranes (Roche, Switzerland) by alkaline transfer ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were size fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred into a polyvinylidene difluoride membrane (Roche, Basel, Switzerland). Membranes were incubated (overnight ...
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bioRxiv - Pathology 2024Quote: ... The digested products were electrophoresed in 1% (w/v) agarose gel and then transferred onto positively charged Nylon membranes (Roche, Germany). For both assays ...
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bioRxiv - Genetics 2022Quote: ... 10 μg total proteins were separated on a 12% SDS-PAGE gel and transferred to the PVDF membranes (Roche, CA, USA) using an electro-transfer apparatus (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... total RNA (50 μg) was separated on 1% agarose–formaldehyde gels and transferred to Hybond-N+ nylon membranes (Roche, Basel, Switzerland). Northern blotting was conducted with biotin-labeled DNA (bio-CTGTAGAAAGTCTGCTGATCGATACCGCGACG ...
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bioRxiv - Genetics 2023Quote: Cell migration assays were performed with the xCELLigence Biosensor System using specifically designed 16-well plates equipped with membranes having 8-μm pores (CIM-plate 16; Roche Diagnostics). Cells in serum-free medium were seeded in the upper chambers ...
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bioRxiv - Molecular Biology 2020Quote: Cell viability was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Roche, Mannheim, Germany), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 μl 1xTaqMan Gene Expression Assay primer and 10 μl 1x FastStart Universal Probe Master-mix (Roche, 04913957001) were applied ...
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bioRxiv - Cancer Biology 2021Quote: ... the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay (11465007001, Roche Diagnostics, Mannheim, Germany) was performed according to manufacturer’s instructions ...
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bioRxiv - Epidemiology 2020Quote: ... Real-time PCR assays were performed with LightCycler® 480 Probe Master Mix 1× (Roche Applied Science, Germany), using 200 nM of primers and probes in a final volume of 12 µl and 2 µl of control DNA were added ...
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bioRxiv - Pathology 2020Quote: ... Radioimmunoprecipitation assay buffer (Biosesang, Inc., Seongnam, Korea) with a protease inhibitor cocktail (Roche Diagnostics, Indianapolis, IN,v USA) was prepared ...
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bioRxiv - Microbiology 2020Quote: Protein was extracted from cells in Radioimmunoprecipitation assay (RIPA) lysis buffer containing 1X Complete Protease Inhibitor Cocktail (Roche) and 1X Phenylmethylsulfonyl fluoride (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell proliferation was assessed by WST-1 assay as per manufacturer’s protocol (Roche Applied Science, Indianapolis, IN, USA). Colony formation assay was performed with MethoCult methylcellulose-based media as per manufacturer’s protocol (Stemcell Technologies ...
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bioRxiv - Physiology 2020Quote: ... The expression of all genes was related to an internal housekeeping gene assay for Actb (Roche, Welwyn, UK) as described previously (O’Hara et al. ...
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bioRxiv - Cancer Biology 2021Quote: For HA pulldown assays the cells were lysed with 500 µL of IP Lysis Buffer containing cOmplete (Roche) protease inhibitor cocktail and incubated at 4°C for 35 min on a rotator followed by centrifugation at 15000 rpm for 15 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... The Roche Elecsys anti-SARS-CoV-2 assay was performed on Roche Cobas e411 (Roche Diagnostics, Indianapolis, IN). The Elecsys antinSARSnCoV-2 assay uses a recombinant protein representing the N antigen for the determination of antibodies against SARSnCoVn2 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Three replicates of each TaqMan® assay were run on a LightCycler® II 480 (Roche, Basel, Switzerland) real time PCR machine in a 384-well format ...
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bioRxiv - Microbiology 2020Quote: Protein was extracted from cells in Radioimmunoprecipitation assay (RIPA) lysis buffer containing 1X Complete Protease Inhibitor Cocktail (Roche) and 1X Phenylmethylsulfonyl fluoride (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: The cytotoxicity assays were performed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Roche, ref:11465007001) protocols according to the previous study [47] with few modifications ...
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bioRxiv - Genomics 2020Quote: ... Specimens in May were tested using the Cobas® SARS-CoV-2 RT-PCR assay (Roche Diagnostics, USA). Respiratory samples archived at the Clinical Laboratory ...
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bioRxiv - Neuroscience 2021Quote: ... PCR primers for the genes of interest were designed by the Universal Probe Library Assay Design Center (Roche) (primer sequences listed in key resource table).
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bioRxiv - Molecular Biology 2022Quote: ... The enriched FLC-Venus protein was detected by western blot assay with the antibody anti-GFP (11814460001, Roche). Signals were visualized with chemiluminescence (34095 ...
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bioRxiv - Pathology 2024Quote: Cholesterol and triglyceride levels were quantified in liver protein lysates and EDTA-plasma using enzymatic colorimetric assays (c.f.a.s. cobas, Roche Diagnostics) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... CMV infection/reactivation was determined by pp65 antigenemia assays prior to 2010 and by CMV qPCR (Roche Diagnostics) from 2010 onward.
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bioRxiv - Microbiology 2022Quote: ... DNA concentrations were estimated using qPCR and the Kapa Library Quantitation Assay for Illumina (Kapa Biosystems, Wilmington, MA). Genomic DNA was sequenced on an Illumina NovaSeq 600 sequencer (Illumina ...
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bioRxiv - Immunology 2023Quote: Total murine plasma cholesterol and triglyceride concentrations were measured with colorimetric assays (CHOD-PAP and CPO-PAP, Roche) using a microplate reader (Infinite F200 PRO ...
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bioRxiv - Microbiology 2024Quote: ... RT-qPCR assays were conducted using Hieff qPCR SYBR green master mix (Yeasen) and qPCR systems from Roche according to the manufacturers’ instructions ...
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bioRxiv - Microbiology 2024Quote: HIV-1 RNA levels were measured in cell-free CSF and plasma at each site using the ultrasensitive Amplicor HIV-1 Monitor assay (versions 1.0 and 1.5; Roche Molecular Diagnostic Systems ...
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bioRxiv - Neuroscience 2024Quote: ... Aβ42 and Aβ40 was measured using Elecsys assays in accordance with the manufacturer’s instructions (Roche Diagnostics International Ltd). CSF Aβ42/Aβ40 was used to define Aβ positivity according to previously established cutoffs of < 0.0855 ...
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bioRxiv - Cancer Biology 2024Quote: ... or TaqMan assays (human ETC genes) on a real-time fluorescence qPCR instrument (LightCycler 480II, Roche Life Science). The expression of relative mRNA was normalized to that of the housekeeping gene 18S ...