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Citations for Roche :
6601 - 6650 of 8217 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... were homogenized in lysis buffer (PBS containing 0.3% Triton X-100, 1 mM sodium orthovanadate and protease inhibitors (Roche, 4693132001). Total protein of each sample was determined using the Bio-Rad DC kit (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... Frozen cell pellets were resuspended in 300 μL of cold hypotonic buffer (10 mM Tris-HCl pH 8, 1.5 mM MgCl2, 1 mM KCl, 10X PhosphoSTOP (Roche, 4906845001), 2X Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and lysed in 50 μL of lysis buffer (50 mM Tris, pH 8, 150 mM NaCl, 1% Triton-X-100, 1x cOmplete EDTA-free protease inhibitor cocktail [Roche]) at 4 °C for 15 min ...
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bioRxiv - Immunology 2024Quote: ... 1 μg of total RNA was reverse transcribed using the Roche Transcriptor First Strand cDNA synthesis kit (Roche Life Sciences) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... antibody in-situ stainings were done as described previously (Haussmann et al., 2008) for validation using rat anti-HA (MAb 3F10, 1:20; Roche), rabbit anti-GFP (Molecular Probes ...
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bioRxiv - Cell Biology 2024Quote: ... dissected TA muscles were first digested with 10ml muscle digestion buffer (DMEM containing 1% penicillin/streptomycin, 0.125mg/ml Dispase II (Roche, 04942078001), and 10mg/ml Collagenase D (Roche ...
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bioRxiv - Immunology 2024Quote: ... and lung lobes were finely minced and incubated in 15 mL of complete media containing 1 mg/mL collagenase (Roche) and 17 U/mL DNase I (Sigma Aldrich ...
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bioRxiv - Genetics 2024Quote: The entire ∼8kb NR2E3 locus was amplified using NR2E3 F and NR2E3 R primers (Table 1) with the Expand Long Template PCR System (Roche) buffer system 1 ...
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bioRxiv - Biophysics 2024Quote: ... 2.0 × 106 cells/mL of WT Neuro2a or OptoTau KI cells were incubated in 10 mM of HEPES buffer supplemented with 1% protease inhibitor (cOmplete, Roche), 1 µM of okadaic acid (Cayman Chemical) ...
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bioRxiv - Immunology 2024Quote: ... the tissue samples were dissected into smaller sections with sterile scalpels and incubated in 100 μl of PBS and 50 μl of Liberase TL (1 mg/ml; Roche) for one hour at +37°C ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were first incubated with 5 µl anti-GFP antibody (in house) for 1 hour rotating at 4°C and subsequently mixed with 50 µl protein G-agarose (50% slurry, Roche) and incubated for 1 hour rotating at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... of RIPA buffer (150 mM NaCl, 50 mM Tris pH 8.0, 1% NP40, 0.5% Na-deoxycholate, 0.1% SDS and Roche complete protease inhibitors) using a Teflon homogenizer ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells were lysed in NP40 cell lysis buffer (10mM Tris-Cl, pH 7.4, 1% NP40, 1X Roche Complete protease inhibitors) at 4C for 1 hour followed by centrifugation at 18,000 g for 10 mins and supernatant collected as sample for analysis ...
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bioRxiv - Genomics 2024Quote: ... Indexing PCR was performed to add Nextera index adapters (1 μM final, Integrated DNA Technology) using KAPA HiFi reagents (#KK2102, Roche). Libraries were pooled in equal volumes and a final 0.8x cleanup was performed with homebrew SeraMag beads before measuring the sample concentration and sizing ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were incubated overnight at 4°C with a primary monoclonal rat anti-HA antibody (1:500; Roche, #11867423001) diluted in 3% BSA in PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... and diluted by adding 800 μl of dilution buffer (16.7 mM Tris-HCl [pH 8.0], 1.2 mM EDTA, 167 mM NaCl, 1.1% Triton X-100, 0.1 mM PMSF, 1% Roche protease inhibitor cocktail tablet). The sample was then sonicated using Bioruptor (Diagenode) ...
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bioRxiv - Molecular Biology 2024Quote: ... we incubated sections 24 minutes at 37⁰C with anti-GFP (1:300, #598, MBL) followed by secondary antibody incubation with OmniMap anti-Rb HRP (760-4311, Roche) at room temperature for 20 minutes ...
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bioRxiv - Immunology 2024Quote: ... incubation buffer containing a peroxidase-labeled anti-DNA mAb (Cell Death Detection ELISA, 11544675001, Roche Life Sciences, dilution 1:10). The plate was incubated for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples used in FISH analyses were incubated in anti-DIG-POD antibody solution (1:2000 dilution; Roche Diagnostics, Mannheim, Germany) rocking overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... The primary antibodies used in this study were an anti-HA rat monoclonal antibody (1:500 dilution, clone 3F10, Roche) and an anti-heat shock protein 70 (HSP70 ...
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bioRxiv - Microbiology 2024Quote: Duplicate aliquots of 1 mL were removed from each flask for subsequent RNA extraction with the High Pure RNA Isolation Kit (Roche). Extraction was conducted following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... 1% Triton X-100, supplemented with protease inhibitor cocktail (cat. #A32965, Pierce) and phosSTOP Phosphatase Inhibitor Cocktail (cat. #4906837001, Roche)) ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with a 20-fold excess of unlabeled oligonucleotide with the same sequence as the labeled one (to prevent reannealing) and 1 µl of Proteinase K (18 mg/ml) (Roche). After deproteination for 30 min at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: Xenograft OS tumors were harvested and microdissected 12 d after 143B OS cell inoculation and digested with collagenase type I and II (1 mg/ml; Roche) digestion for 45 min ...
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bioRxiv - Neuroscience 2024Quote: ... Larvae were blocked and then incubated with 1/4000 concentration of anti-DIG Fab fragment conjugated with alkaline phosphatase (Roche) overnight at 4ºC ...
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bioRxiv - Neuroscience 2024Quote: ... The mixture for qPCR reaction was prepared in a final volume of 20 μl containing 1 μl cDNAs and 10 μl of LightCycler 480 SYBR Green I Master (Roche) in the presence of primers at 500 nM ...
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bioRxiv - Plant Biology 2024Quote: Seed histological sections were washed with Phosphate Buffered Saline solution (PBS) and digested with 1 mg/ml proteinase-K (Hoffmann-La Roche). Subsequently ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were washed 3x 15 minutes in room temperature TBST (Tris-buffered saline with 1% Tween 20) and blocked at room temperature for one hour in 10% DIG buffer (Roche) in TBST.
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bioRxiv - Immunology 2024Quote: ... Quantitative RT-PCR analysis was performed with selective primers (Supplementary Table 1) using the Light Cycler 480 SYBR Green I Master X2 Kit (Roche), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Probed membranes were washed and incubated with horseradish peroxidase-conjugated anti-mouse or anti-rabbit Abs for 1 hour and developed with Lumi-LightPLUS Western Blotting Substrate (Roche). Quantification was performed on Lumi-Light autoradiography films using ImageJ software.
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bioRxiv - Immunology 2024Quote: Cell lysates were prepared by lysing cells for 1 h at 4 °C in RIPA buffer supplemented with cOmplete protease inhibitor cocktail (Roche). The cell lysate concentrations were quantified using a BCA protein assay kit (ThermoFisher) ...
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bioRxiv - Cancer Biology 2024Quote: ... Total proteins from T-ALL#1 cells were extracted with a RIPA buffer containing a cocktail of 1Xprotease inhibitors (11836145001, Roche) and 1X phosphatase inhibitors (P2850 ...
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bioRxiv - Immunology 2024Quote: ... whole pituitaries or separate posterior and anterior pituitaries were mechanically lysed by suspending and digested with 50 µg/ml DNase 1 (Roche) and 1 mg/ml Collagenase D (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... life-Tein LLC) was mixed with 50µl of PBS containing 1% Tween20 and 5ng/mL anti-HA-Peroxidase 3F10 antibody (Roche #12013819001) and transferred to the wells ...
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bioRxiv - Bioengineering 2024Quote: ... or 1 M NaCl (for ori designs), 20 µg/mL DNase (#A3778, ITW Reagents) and protease inhibitor cocktail (04693132001, Roche)) ...
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bioRxiv - Neuroscience 2024Quote: 1 µg of each total RNA sample was used to synthetize cDNA with Transcriptor First Strand cDNA synthesis Kit (Roche) according to the manufacturer’s instructions with the additional denaturation step at 65°C for 10 min ...
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bioRxiv - Neuroscience 2024Quote: Cells were harvested using a lysis buffer (TBS with 1% Triton X-100, 1x Roche EDTA free cOmplete protease inhibitor, 1x Roche pSTOP phosphatase inhibitors ...
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bioRxiv - Cancer Biology 2024Quote: ... desoxycholic acid-Na-salt 1%, SDS 0.1%) (Serva electrophoresis, Germany) containing protease inhibitor cocktail and phosphatase inhibitor cocktail (Roche, Switzerland). Western blots were performed following routine protocols67 ...
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bioRxiv - Plant Biology 2024Quote: ... 1 mL of the total protein extract was incubated with 20 μL of Anti-HA Affinity Matrix (rat IgG1, Roche) or Anti-FLAG M2 Affinity Gel (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... cells were lysed in a NP-40 buffer (50 mM Tris, 150 mM NaCl, 1% Nonidet P-40, and Complete Mini protease inhibitor cocktail (Roche), Flag-tagged M117 protein was precipitated with anti-Flag and protein G sepharose ...
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bioRxiv - Systems Biology 2024Quote: ... Each cell pellet was resuspended in roughly 0.9 ml of lysis buffer consisting of 1% SDS and 200 mM EPPS with protease inhibitors (Roche 11836170001). The cell mixture was boiled at 95°C for 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... solution then stained for 12 - 18 hours at 4°C in buffer containing 10% HISS and alkaline phosphatase-conjugated sheep anti-digoxigenin antibody (1:2000, Roche).
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bioRxiv - Biochemistry 2024Quote: ... and 0.5% sodium deoxycholate,100 µL buffer and ∼20 µL beads per 10 mg tissue) with 1 x PhosSTOP phosphatase inhibitor cocktail (Roche). ∼50 µg protein from each sample was then digested in two steps by Lys-C and trypsin ...
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bioRxiv - Molecular Biology 2024Quote: ... FISH probes were made by labelling 1 μg of DNA with BIO-nick translation kit or DIG-nick translation kit (Roche). Following addition of hybridization mix slides were incubated in a thermocycler with the following program ...
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bioRxiv - Genomics 2024Quote: ... Probes were added together (each at a 1:50 dilution) to hybridization buffer (70% deionized formamide, 10 mM Tris-HCl pH 7.5, 0.5% 10x Roche blocking solution). Diluted probes were denatured at 90°C and coverslips were denatured at 85°C ...
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bioRxiv - Cell Biology 2024Quote: ... resuspended in with TritonX-100 buffer (1% Triton X-100, 0.5 M EDTA, PBS, ddH2O) with protease/phosphatase inhibitors (cOmplete™ Protease Inhibitor Cocktail (Roche), PhosSTOP phosphatase inhibitor cocktail (Roche) ...
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bioRxiv - Genomics 2024Quote: ... 1 μg of total RNA from each sample was converted into cDNA with First Strand cDNA Synthesis Kit (Roche, #04897030001). The qPCR was performed using LightCycler 480 SYBR Green I Master (Roche ...
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bioRxiv - Systems Biology 2024Quote: ... then primary antibodies or lectins added at the following dilutions in IF blocking buffer: rat anti-HA 1:1000 (Roche), biotinylated GNL 1:1000 (Vector Labs) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... beads were washed between 10-15 times with wash buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1 mM DTT, and protease inhibitors, cOmplete Mini, EDTA-free, Roche, #4693159001). and ...
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bioRxiv - Neuroscience 2024Quote: ... mouse brains were homogenized at a 1:10 (w/vol) ratio of the brain in 1X TBS supplemented with protease inhibitor cocktail (Roche), using a Bead Beater (1 min ...