Labshake search
Citations for Roche :
6551 - 6600 of 8217 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... then lysed on ice for 15 minutes in non-denaturing lysis buffer (150 mM KCl, 10 mM MgCl2, 5mM HEPES, 1% IGEPAL) supplemented with protease inhibitors (Roche Complete EDTA-free ...
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bioRxiv - Neuroscience 2022Quote: ... and proteins were transferred to an activated (100% ethanol, 1 min; followed by two washing steps with water) PVDF membrane (Roche Diagnostics GmbH ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM TCEP 10 % v/v glycerol) supplemented with 4x EDTA-free protease inhibitor cocktail tablets (Roche, cat. No. 11836153001) and 4 μg/ml DNase I ...
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bioRxiv - Developmental Biology 2022Quote: ... before being incubated in TNTw/block for 1 hour followed by an ON incubation with anti-DIG or anti-Fluo horseradish peroxidase (Roche). Post-antibody washes and the TSA reactions were repeated as for the first probe ...
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bioRxiv - Cell Biology 2022Quote: ... Thawed pellets were resuspended with 200-300 µL native lysate buffer (1% NP-40 with 1X cOmplete protease inhibitor cocktail [SKU, 11836145001, Roche] ...
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bioRxiv - Cell Biology 2022Quote: ... At the completion of the assay islets were lysed in RIPA buffer (Pierce) containing 1 mM PMSF and EDTA-free protease inhibitor cocktail (Roche)) at 95°C for 5 minutes ...
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bioRxiv - Cell Biology 2022Quote: Protein lysates were collected from worms using RIPA buffer (50 mM Tris-HCl, 250 mM sucrose, 1 mM EDTA, and Roche protease inhibitor tablet ...
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bioRxiv - Biophysics 2022Quote: ... cells were gently washed twice with 500 μl cold PBS and directly lysed in 50 μl of lysis buffer (PBS, 1% (v/v) Triton X-100 and protease inhibitor cocktail (Roche)) ...
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bioRxiv - Cancer Biology 2022Quote: ... at 1:500 for 32 min was used followed with the secondary antibody OmniMap anti-rabbit HRP (760-4311, Roche). Antigen-antibody complexes were revealed with ChromoMap DAB Kit (Roche ...
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bioRxiv - Biochemistry 2022Quote: ... 150 ng of U1 RNA (TMG or NAD cap) and 150 ng of HIV-1 mRNA were mixed with ResoLight dye (1x, Roche) and annealing buffer (10 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: Mouse pancreata from adult mice (9-14 week old) were perfused with a solution containing 1 mg/mL collagenase-P (Roche) in Hank’s buffered salt solution (HBSS ...
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bioRxiv - Cancer Biology 2022Quote: ... The eluted chromatin was then treated with RNaseA (10mg/mL) for 1 hour at 37°C and with Proteinase K (Roche) for 2 hours at 55°C ...
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bioRxiv - Cell Biology 2022Quote: ... Both Caco2 and HepG2 cells were transfected with 1 µg of pooled plasmid using X-tremeGENE9 Transfection Reagent (Roche:6365779001) following manufacturer recommendations ...
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bioRxiv - Cell Biology 2022Quote: ... isolated from each cell line was collected by centrifugation (30 000 x g, 4°C, 10 min) and resuspend in 1 x PBS supplemented with protease inhibitors (Roche) to protein concentration 1.5 mg/ml ...
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bioRxiv - Developmental Biology 2022Quote: LC-MS-based quantification of methyl-cytosines was performed on 1 μg of DNA degraded to nucleosides with nuclease P1 (Roche), snake venom phosphodiesterase (Worthington ...
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bioRxiv - Genetics 2022Quote: ... COS-7 cells transiently expressing POPDC constructs with the appropriate epitope tag were lysed using a 1% (v/v) Triton X-100 based lysis buffer supplemented with cOmplete protease inhibitor cocktail (Roche). Lysates were subjected to Co-IP using the ProFoundTM c-Myc Tag IP/co-IP Kit (Thermo Scientific ...
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bioRxiv - Evolutionary Biology 2022Quote: Slides were blocked for two hours at room temperature in blocking buffer composed of 1% Roche blocking reagent (Roche #11096176001) and 20% sheep serum ...
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bioRxiv - Developmental Biology 2022Quote: ... and 0.25 µM forward and reverse primers (Extended Data Table 1) on a LightCycler 480/II 384 (Roche, serial #6073). Four technical replicates were performed per biological replicate qPCR primer set ...
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bioRxiv - Microbiology 2022Quote: ... lacking the predicted signal peptide (amino acids 1-29) was amplified from genomic DNA (isolated using the High Pure Template kit, Roche) by PCR using the Phusion Hot start II High Fidelity DNA Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2022Quote: ... Sections after the post-hybridization washing and blocking steps were incubated with peroxidase-conjugated anti-digoxigenin sheep antibody (1:1000; 11207733910, Roche) in TS 7.5 containing 1% Blocking Reagent at 4°C for overnight ...
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bioRxiv - Microbiology 2022Quote: ... Beads were then washed three times with 400 μL wash buffer (25 mM Tris-HCl pH 8, 150 mM NaCl, 1 mM EDTA pH 8, cOmplete protease inhibitor cocktail (Roche), 5% glycerol) ...
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bioRxiv - Microbiology 2022Quote: ... A cell pellet was resuspended in 250 μL Tris-EDTA buffer and 50 μL 30 g L-1 lysozyme (Roche) in Tris-EDTA buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... 30 mM imidazole and 10 % (v/v) glycerol] supplemented with 1 mM PMSF and complete EDTA-free protease inhibitor cocktail (Roche). After centrifugation at 45 000 x g supernatant was filtered (0.45 µm ...
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bioRxiv - Plant Biology 2022Quote: ... 10% [v/v] glycerol, 10 mM EDTA, 1% [v/v] NP-40, and complete EDTA-free protease inhibitor cocktail [Roche]). The extracts were centrifuged at 20,000 × g at 4°C for 15 min ...
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bioRxiv - Plant Biology 2022Quote: ... then ground in 500 μl freshly prepared lysis buffer (25 mM Tris HCl pH 8, 150 mM NaCl, 1% SDS, 1x cOmplete ULTRA protease inhibitor (Roche), and 1x PhosSTOP (Roche)) ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were incubated with 8-keto-NeuAc derivatives for 24 h and proliferation measured using WST-1 (Roche Applied Science) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μg of total RNA from each sample was converted into cDNA with First Strand cDNA Synthesis Kit (Roche, #04897030001). Primers used in the RT-qPCR are listed in Supplementary Table S1 ...
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bioRxiv - Molecular Biology 2023Quote: ... upon which cells were washed with 2x ice-cold PBS and lysed in RIPA buffer with 1× EDTA-free protease inhibitor cocktail (Roche). Lysates were then separated on a 17.5% acrylamide gel for 6h at 150V and transferred onto a 0.22µM nitrocellulose membrane using semi-dry transfer ...
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bioRxiv - Molecular Biology 2023Quote: ... and each was lysed by trituration in 1 mL ice-cold PBS with 0.25% Triton-X containing cOmplete™ mini EDTA-free protease inhibitor cocktail tablet (Roche) at a concentration of 10% w/vol followed by a 15-minute incubation on ice ...
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bioRxiv - Biochemistry 2022Quote: ... 0.05% (v/v) Tween-20) supplemented with 10 μg/mL DNase I and 1 cOmplete protease inhibitor cocktail tablet (Roche) per L buffer ...
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bioRxiv - Cell Biology 2023Quote: Parental and Haspin knockout cells stably expressing GFP-TAF5 were obtained by cotransfection with pcDNA5/FRT/TO/GFP-TAF5 (Antonova et al., 2018) and a pPGKpuro resistance plasmid (in a 9:1 ratio) using Xtreme Gene 9 (Roche). After 48 h ...
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bioRxiv - Plant Biology 2023Quote: ... the AIRs were treated with 1 unit per AIR mg of type I α-amylase (Roche 10102814001, Roche GmbH, Germany). The de-starched AIRs ...
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bioRxiv - Cell Biology 2023Quote: ... The microsome fraction was solubilized in 1% Triton X-100 in PBS (10 mg microsome per ml) with protease inhibitor cocktail (complete mini, Roche) by rocking at 4°C for 60 min ...
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bioRxiv - Cell Biology 2022Quote: HeLa cells in 10-cm Petri dishes were lysed in 1 ml RIPA buffer (100 mM Tris HCl, pH 7.5, 150 mM NaCl, 1% Igepal, 0.1% deoxycholate, 0.1% SDS, supplemented with Roche protease inhibitor cocktail), under constant rotation for 30 min at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... the cells washed twice with cold PBS and then scraped from the cell culture dishes in 1 mL PBS with cOmplete protease inhibitor by Roche. The cells were washed once with lysis buffer 1 (140 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... and cells were scraped in an ice-cold homogenization buffer (10 mM Tris-HCl pH 7.4, 1 mM EGTA, 0.5 mM EDTA, 0.25 M sucrose, supplemented with Roche protease inhibitor cocktail) and homogenized with ten strokes of a loose-fitting Dounce homogenizer ...
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bioRxiv - Cell Biology 2023Quote: ... Tissues were incubated in digestion buffer (0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum) for 45 minutes at 37°C while agitating at 750 rpm on an Eppendorf ThermoMixer ...
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bioRxiv - Genomics 2022Quote: ... RNA-seq libraries were prepared using 1 μg of total RNA input using KAPA stranded mRNA-seq kit for Illumina (Roche) as per manufacturer’s instruction and paired-end sequenced on NovaSeq 6000 (Illumina).
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bioRxiv - Cell Biology 2023Quote: Cell pellets were lysed in TBS-T lysis buffer (1% Triton X-100 in 50 mM Tris-HCl, 150 mM KCl, and a cocktail of protease inhibitors (Roche)) at 4°C and incubated for 1 hr with intermittent vortexing ...
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bioRxiv - Biochemistry 2023Quote: The cell pellets were re-suspended in 2 ml of mitochondrial isolation buffer (200 mM sucrose, 10 mM Tris HCl, 1 mM EGTA/Tris, protease inhibitor cocktail (Roche), pH7.4 (adjusted with 1M HEPES)) ...
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bioRxiv - Biochemistry 2023Quote: ... The pellet was resuspended in 30 mL of 40 mM Tris pH 8 supplemented with 1 tablet of EDTA-free protease inhibitor tablet (Roche), and 0.1 mM PMSF ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was then washed and incubated 1 hs at RT with goat anti-mouse secondary antibody conjugated to HRP (Roche) at 1/20000 dilution in PBST - 5% milk.
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bioRxiv - Developmental Biology 2023Quote: ... Larvae were hybridized with digoxigenin (DIG)-labeled riboprobes overnight at 65°C and incubated overnight with 1/2000 alkaline phosphatase-conjugated anti-DIG Fab fragment (Roche) at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: RNA sequencing (RNAseq) libraries were prepared using 1 µg of total RNA using the KAPA stranded mRNA-seq kit (Roche) according to the manufacturer’s specifications ...
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bioRxiv - Plant Biology 2023Quote: ... the AIRs were treated with 1 unit per AIR mg of type I α-amylase (Roche 10102814001, Roche GmbH, Germany). The de-starched AIRs ...
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bioRxiv - Genetics 2023Quote: To extract genomic DNA cells were resuspended in genomic lysis buffer (300 mM NaCl, 1% SDS, 20 mM EDTA) and incubated with Proteinase K (Roche) at 55 °C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... CVF samples were normalized to a protein concentration of 1 mg/mL using sterile PBS and EDTA-free protease inhibitor cocktail (Roche) was added ...
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bioRxiv - Physiology 2023Quote: ... in PEB Buffer (150 mM NaCl, 50 mM Tris-HCl, 5mM EDTA, 1% NP-40) containing proteases (cOmplete™, Roche) and phosphatase (Phosphatase Inhibitor Cocktail 2 ...
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bioRxiv - Plant Biology 2023Quote: ... After nuclei were resuspended in RIPA buffer (1x PBS, 1% IGEPAL CA-630, 0.5% Sodium deoxycholate, 0.1 % SDS, Roche Complete tables) and the DNA was fragmented to 200-500 bp by sonication ...
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bioRxiv - Cell Biology 2023Quote: Cells were plated in 96-well plates and treated as indicated for 72 hours before performing MTT survival assay using Cell Proliferation Kit 1 MTT (Roche) following the manufacturer’s instructions ...