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Citations for Roche :
6451 - 6500 of 9754 citations for Monoisodecyl Phthalate 100 Ug Ml In Mtbe Unlabeled Mono 3 7 Dimethyl 1 Octyl Phthalate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Cells were pelleted and washed with 1 pellet volume of cold RP buffer (retromer purification buffer: 50 mM Tris pH 8.0, 300 mM NaCl, 1 mM MgCl2, 1 mM PMSF, Roche complete protease inhibitor).
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bioRxiv - Developmental Biology 2022Quote: ... The samples were then washed with 0.1% PBST and incubated in 1% blocking buffer (1% blocking reagent [Roche, 11175041910] in Maleic acid) for 1 h ...
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bioRxiv - Genetics 2019Quote: ... resuspended in 60 ml hypotonic lysis buffer (20 mM HEPES-KOH pH 7.5, 5 mM NaCl, 1 mM MgCl2, 1 mM PMSF and EDTA free protease inhibitor mixture from Roche and Thermo Scientific) and incubated for 15 min on ice ...
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bioRxiv - Molecular Biology 2020Quote: ... 15 mM MgOAc, 15 mM Tris-HCl pH 7.4, 0.5% NP40, 1 mM DTT, 1 μl RNAsin, Roche protease inhibitors without EDTA). The cells were lysed by one freeze-thaw cycle in large volume (15–50 ml ...
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bioRxiv - Cell Biology 2020Quote: ... The remaining cell pellet was weighed and resuspended in a 1:1 ratio with ice-cold 1x PBS with a protease inhibitor tablet (Roche Life Sciences) and 1mM PMSF ...
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bioRxiv - Physiology 2021Quote: ... 1:500 goat anti-rabbit (AlexaFlour 488; A-11008) and 1:1000 4′,6-diamidino-2-phenylindole (DAPI; Roche 10236276001; Basel, Switzerland). After secondary incubations ...
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bioRxiv - Cell Biology 2022Quote: ... three hearts per experimental group were homogenized into ice-cold isolation buffer (300 mM KCl, 30 mM PIPES pH 6.6, 0.5% NP-40, 1× protease inhibitor [Roche], 1× phos-stop [Roche]), followed by centrifugation at 15,000 rpm for 10 minutes in a tabletop centrifuge at 4°C to remove insoluble proteins ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM β-glycerophosphate and 1% Triton) containing protease inhibitor cocktail (Rowe Scientific; CP2778) and PhosSTOP EASYpack Phosphatase Inhibitor Cocktail (Roche; 4906837001) on ice for 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Five hunderd mL Lysis Buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% SDS, 1 mM PMSF, and EDTA-free protease inhibitor cocktail from Roche, dissolved in PBS) was added and cells were harvested from the Transwell membrane using a disposable cell scrapper and transferred to an Eppendorf tube ...
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bioRxiv - Neuroscience 2022Quote: Cells were homogenized in 1 % NP40 lysis buffer (20 mM HEPES pH7.5, 150 mM NaCl, 1 % NP-40, 10 % Glycerol and Roche protease inhibitor mix) on ice ...
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bioRxiv - Neuroscience 2022Quote: ... 1% sodium deoxycholate, 50 mM NaF, 2 mM EDTA, 2 mM DTT, 0.2 mM Na orthovanadate, 1 X Roche protease inhibitor #11836170001). Lysates were sonicated and centrifuged to remove debris ...
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bioRxiv - Neuroscience 2022Quote: ... Coronal sections containing the medial prefrontal cortex (mPFC) or hippocampus were incubated with (1) an antibody cocktail of rat anti-HA (1:200, Cat. No. 10145700, Roche Diagnostics, USA) with rabbit anti-PV (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were pelleted and washed with 1 pellet volume of cold RP buffer (Retromer purification buffer: 50 mM Tris pH 8.0, 300 mM NaCl, 1 mM MgCl2, 1 mM PMSF, Roche complete protease inhibitor). Pellets were either processed immediately or flash-frozen in liquid nitrogen and stored at -80°C ...
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bioRxiv - Cell Biology 2024Quote: ... the full-length Treacle was amplified by PCR from cDNA with primer set #1 (Supplementary Table 1) using KAPA High-Fidelity DNA Polymerase (KAPA Biosystems, KE2502). The forward and reverse primers contained XhoI sites ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were stained with secondary antibodies (1:300) for 2 hours at room temperature and nuclei stained with DAPI (1:1000, Roche, Munich, Germany). The cells were mounted using the ProLong Diamond antifade mountant (Thermo ...
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bioRxiv - Cell Biology 2023Quote: ... Nuclei pellets were resuspended in 100 µl of nuclei lysis buffer (20 mM HEPES pH 7.9, 0.1 M NaCl, 1mM EDTA, 1 mM EGTA, 1mM DTT, 25 % glycerol, 0.5 mM AEBSF, 1×Roche complete protease inhibitor cocktail) for 20 min at 4 °C with rotation ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was extracted using NETN buffer (20 mM Tris-HCl pH 8, 420 mM NaCl, 1 mM EDTA, 0.5% IGEPAL, 1 mM DTT, and Roche Protease Inhibitor Cocktail) and were resolved in 8% Bis-Tris SDS-PAGE gels ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM EDTA pH 8 and 10 mM NaF completed with 1 × protease inhibitor cocktail (Pierce, #A32955) and 1 × phosphatase inhibitor cocktail (Roche PhosSTOP, #490684001). The total protein concentration was determined using Bradford assay (Protein Assay Reagent ...
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bioRxiv - Neuroscience 2024Quote: ... 150 mM NaCl, 1 mM CaCl2, 1 mM MgCl2, 0.5% NP-40 [Thermo Scientific, Waltham, MA, USA], EDTA-free protease inhibitor [Roche, Basel, Switzerland]). Lysed astrocytes were collected into 1.5 mL microcentrifuge tube ...
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Conserved Cis-Acting Range Extender Element Mediates Extreme Long-Range Enhancer Activity in MammalsbioRxiv - Genomics 2024Quote: ... to prepare for an hour long blocking in 10% lamb serum/TBST and an overnight incubation at 4°C in a 1% lamb serum containing Anti-Dig-AP antibody (Roche, 1:5000). The next morning ...
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bioRxiv - Biophysics 2021Quote: ... Primary antibodies were: rat-anti-HA (1:5,000; Roche Applied Science catalog ...
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bioRxiv - Cancer Biology 2021Quote: ... 5mM EDTA) with 1× cOmplete protease inhibitors (Roche 11697498001), incubated on ice for 20 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... 1 cOmplete EDTA-free protease inhibitor cocktail tablet (Roche), after which the lysate was clarified by centrifugation at 30,000 x g for 60 min at 4 °C in a JA 25.50 rotor in an Avanti J-E centrifuge (Beckman) ...
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bioRxiv - Cell Biology 2019Quote: ... mouse monoclonal anti-GFP (Roche, 11814460 001, 1:2000), monoclonal mouse anti V5 (Invitrogen R960-25 ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM NaVO3 and a protease inhibitor tablet (Roche)) ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 tablet miniComplete EDTA free protease inhibitor (Roche) per 10 mL ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by biotinylated donkey-anti-sheep (1/500, Roche). ABC kit from Vector Laboratory was used followed by fluorescent tyramide staining (Alexa fluor 555 or 488 conjugated tyramide from Molecular Probes) ...
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bioRxiv - Developmental Biology 2020Quote: ... A 1/2500 dilution of anti-DIG (Roche, 1109327490) in 1x MABT + 20% heat inactivated sheep serum was then added and slides were placed in a humified chamber and incubated overnight at room temperature ...
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bioRxiv - Genomics 2020Quote: ... 1% sodium deoxycholate) containing 1x protease inhibitor cocktail (Roche). Next ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 x EDTA-free Complete Protease Inhibitor Cocktail (Roche) and 110 U/ml of RNasin (Promega) ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated with DAPI (1:10000, Roche) for 5 min and imaged using a fluorescent microscope (Olympus BX51) ...
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bioRxiv - Neuroscience 2021Quote: ... and mouse anti-GFP antibody (1:500, Roche, RRID:AB_390913) diluted in TNB was added to each slide under a bridged coverslip and incubated at 4°C overnight ...
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bioRxiv - Neuroscience 2021Quote: ... rat monoclonal anti-HA (Roche, clone 3F10, 1:1,000), and guinea pig polyclonal anti-PSD-95 (Frontier Institute ...
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bioRxiv - Neuroscience 2021Quote: ... a concentration of 1:500 anti-GFP (Roche 11814460001) and 1:5000 anti-mouse HRP (Millipore AP308P ...
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bioRxiv - Neuroscience 2021Quote: ... and 1 uL of anti-GFP antibody (Roche 11814460001) were used to pull down DMA-1::GFP and KPC-1::GFP overnight at 4C ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse anti-GFP (7.1+13.1, Roche, 11814460001; 1:1000), rabbit anti-Caveolin (BD Biosciences 610059 ...
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bioRxiv - Molecular Biology 2021Quote: ... 1% NP-40 and COMPLETE protease inhibitor cocktail (Roche). Equal amounts of crude cell extracts (100 µg total protein ...
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Proteasome granular localization is regulated through mitochondrial respiration and kinase signalingbioRxiv - Cell Biology 2022Quote: ... Antibodies used were anti-GFP (1:500; Roche, #11814460001), and anti-Pgk1 (1:10,000 ...
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bioRxiv - Genomics 2020Quote: ... 1× Protease Inhibitor Cocktail (Roche, 11 873 580 001)] and incubation at 12 °C on a ThermoMixer for 30 min ...
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bioRxiv - Genetics 2019Quote: ... 1% NP40) supplemented with protease inhibitors (Roche Mini tablets). The tissue was disrupted with a plastic pestle and incubated by end-over-end rotation for 15 minutes at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... 1/10 volume of Protein-G agarose beads (Roche) was then added and the sample rotated for 30 min at RT °C ...
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bioRxiv - Genomics 2020Quote: ... 1% sodium deoxycholate) containing 1x protease inhibitor cocktail (Roche), incubated for 20 min on ice ...
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bioRxiv - Biochemistry 2019Quote: ... 1× cOmplete EDTA-free protease inhibitor (Roche, Basel, Switzerland), 100 mM ATP ...
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bioRxiv - Cell Biology 2019Quote: ... and 1 cOmplete Protease Inhibitor Cocktail tablet (Roche Diagnostics) added prior to lysis ...
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bioRxiv - Biochemistry 2019Quote: ... mouse-anti-GFP (1:1000) (Roche Cat# 11814460001, RRID:AB_390913), and rabbit-anti-Hxk1 (1:3000 ...
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bioRxiv - Cell Biology 2020Quote: ... 1% NP-40) supplemented with protease inhibitor cocktail (Roche). Protein concentration was determined using bicinchoninic acid (BCA ...
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bioRxiv - Cell Biology 2019Quote: ... 1:500 Rat anti-HA (Roche Diagnostics, Indianapolis, IN), 1:500 Rat anti-C-Myc (BioRad ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 mM Pefabloc and Complete Protease Inhibitor Cocktail (Roche). Lysis was carried out at 4°C for 20 min followed by 10 min centrifugation at 18,000g at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... 1 tablet of complete Inhibitor cocktail EDTA free (Roche), per 50ml ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM DTT) supplemented with Complete protease inhibitor (Roche). Lysates were rotated at 4°C for 20 minutes prior to maximum speed centrifugation at 4°C ...