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Citations for Roche :
6251 - 6300 of 9754 citations for Monoisodecyl Phthalate 100 Ug Ml In Mtbe Unlabeled Mono 3 7 Dimethyl 1 Octyl Phthalate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... and resuspended in 100 μl of protein breakage buffer (10 mM Tris-HCl pH 7.5, 1 mM EDTA pH 7.5, 2.75 mM DTT, 1 x Roche EDTA-free protease inhibitors) and one volume of glass beads (0.5mM zirconia/silica glass beads ...
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bioRxiv - Microbiology 2021Quote: ... 1mM EDTA, pH 8.0; 1 mM EGTA, pH 8.0; 0.65% NP-40; 1 mM DTT, 1x Roche Complete protease inhibitors cocktail). Nuclei were pelleted at 3,000 x g for 20min at 4°C and cytoplasmic fraction was removed ...
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bioRxiv - Molecular Biology 2020Quote: ... 200 mM NaCl, 2 mM MgAc2, 1% [w/v] LMNG, 1 mM DTT, 1x complete EDTA-free protease inhibitor cocktail [Roche, Germany]). After 30 min ...
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bioRxiv - Immunology 2022Quote: ... counted and lysed in m-RIPA buffer (1% NP40, 1% sodium deoxycholate, 150mM NaCl, 50mM Tris pH 7.5, Complete protease inhibitors; Roche, Basel, Switzerland). CANX and T6BP were immunoprecipitated using anti-CANX antibody PA5-34754 (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: The following conjugated antibodies were used: sheep polyclonal anti-digoxigenin alkaline phosphatase (1:2000 - 1:5000; Roche Life Science, Cat#11093274910), mouse monoclonal anti-βACTIN (clone AC-15 ...
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bioRxiv - Physiology 2020Quote: ... in lysis buffer (50 mM Tris, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.5% Na-deoxycholate, 0.1% SDS, protease inhibitors [Roche], phosphatase inhibitors [Roche]). Lysates were incubated for 15 minutes on ice and centrifuged twice at 15,000 x g for 20 minutes at 4°C to remove insoluble material ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant was again discarded and 0.6 ml Storage buffer (10% glycerol, 1 mM EDTA, 10 mM TrisHCl pH 7.5, 25 mM NaCl, 1 mM PMSF, 1x Roche protease inhibitor) was added on top of the pellet ...
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bioRxiv - Systems Biology 2021Quote: ... HEK293 cells were lysed in the lysis buffer (150 mM NaCl, 50 mM Tris pH 7.5, 1 % Triton, 1 x EDTA-free Roche protease inhibitors) and incubated on ice for 30 mins prior to a double sonication at 12 % for 3 seconds each (1 min on ice between sonications) ...
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bioRxiv - Microbiology 2021Quote: ... falciparum ACT1 (final dilution 1:500; a kind gift from Jake Baum) or rat anti-HA (1:1000; Roche, Basel, Switzerland) for HA-tagged GAP45 and AMA1 ...
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bioRxiv - Immunology 2020Quote: ... 0.1% NP-40 (Nonidet P40, Applichem) and 1 pill of protease inhibitor EDTA-free (cOmplete Tablets, Mini EDTA free, Roche diagnostics) per 30 mL lysate ...
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bioRxiv - Biophysics 2019Quote: ... cells were harvested by centrifugation and resuspended in lysis buffer (20 mM Tris pH 7.5, 1 mM EDTA, 200 mM NaCl, 1 mM βMe, Roche protease inhibitor) and frozen ...
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bioRxiv - Microbiology 2020Quote: ... from persons with established infection (both ART naïve and receiving ART) with HIV-1 VL data (COBAS AmpliPrep/COBAS TaqMan® HIV-1 Test, v2.0, Roche Molecular Diagnostics) were included to evaluate the impact of VL on the performance of the ARCHITECT.STOP was a prospective study evaluating the recommended diagnostic algorithm for detection of acute HIV infection and linkage to enhanced partner services in New York City ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were lysed for 10 min on ice in 3 ml ChIP lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris pH 8.1, 1 mM PMSF, 1,5x Roche protease inhibitor mix). The DNA was sheared by sonication (4x 7 cycles ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 h at room temperature and incubated with primary antibodies overnight at 4°C against either HA (1:5000, Roche 12013819001), FLAG (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... and incubated overnight with primary antibody (monoclonal anti-FLAG, 1:1000; Sigma-Aldrich, #F1804-1MG; or anti-GFP, 1:1000, Roche #11814460001 in 3% bovine serum albumin (BSA)/TBS-T ...
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bioRxiv - Plant Biology 2023Quote: ... pH 7.9, 50% glycerol, 75 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 1 mM PMSF and Roche protease inhibitor cocktail), then 100 μl prechilled nuclei lysis buffer (10 mM HEPES ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were then washed with 0.1% PBST and incubated in 1% blocking buffer (1% blocking reagent [Roche] in Maleic acid) for 1 h ...
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bioRxiv - Plant Biology 2023Quote: ... 50 mM Tris/HCl pH7.5, 1 mM EDTA, 300 mM NaCl, 1 mM DTT, 0.1% [v/v] Nonidet P-40/Igepal, Roche cOmpleteTM protease inhibitor) was added to grinded-frozen samples ...
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bioRxiv - Biochemistry 2023Quote: ... and 0.1% SDS) with 1 mM phenylmethylsulfonyl fluoride (PMSF) and 1 X EDTA-free mammalian protease inhibitor cocktail (Roche Diagnostics, 11836170001). Cell pellets from control and subject fibroblasts and corresponding overexpression cells were solubilized in 1% n-dodecyl-β-D-maltoside (DDM ...
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bioRxiv - Cell Biology 2022Quote: ... 200 mM NaCl; 2 mM MgAc; 1% [w/v] LMNG, 1 mM DTT, 1x complete EDTA-free protease inhibitor cocktail [Roche, Germany]) per 1 g of cell pellet and incubated for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and incubated overnight at 4°C in 1% goat serum in MABT with 1∶5000 alkaline phosphatase-coupled anti-Digoxigenin antibody (Roche Diagnostics). On the third day ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The libraries were diluted 1:10 000 and 1:20 000 and quantified in triplicate using the KAPA Library Quantification Kit (Kapa Biosystems). Equimolar samples were pooled ...
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bioRxiv - Cancer Biology 2023Quote: ... washed in ice-cold PBS and lysed (1% SDS, 10 mM Tris-HCl, 1 mM NaVO3, cOmpleteTM Mini Protease Inhibitor (Roche, #11836153001), pH 7.5 ...
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bioRxiv - Developmental Biology 2024Quote: ... the samples were incubated for 1.5h in PBSTw-1%BSA before being incubated with anti-DIG-POD antibody (Roche, 1:2000) and other primary antibodies o/n at 4°C ...
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bioRxiv - Biophysics 2024Quote: ... Cultures were grown until an A600 of 0.6-0.8 was reached and protein over-expression was induced by incubating with 1 mM isopropyl-β-d-1-thiogalactopyranoside (IPTG, Roche) for 16 hours at 18°C.
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Tumor microenvironment acidosis favors pancreatic cancer stem cell properties and in vivo metastasisbioRxiv - Cancer Biology 2024Quote: ... Cells were grown to ∼80% confluency, washed in PBS and lysed (1% SDS, 10 mM Tris-HCl, 1 mM NaVO3, and protease inhibitors (Roche, #11836153001), pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... Pellets were suspended in 50 ml of denaturation buffer (8 M GdmCl, 50 mM Tris-HCl, pH 8.0) with one protease inhibitor tablet (Roche) per L medium ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was collected and incubated with 20 ml of Eps8L2 Lysis Buffer without lysozyme nor Pefabloc® (Roche), and 4% Triton X-100 for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... mechanically disintegrated and partially digested in a solution of collagenase and trypsin [2 mg/ml collagenase (Roche or Sigma), 2 mg/ml trypsin (Dutcher Dominique or Sigma) ...
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bioRxiv - Cell Biology 2020Quote: Genomic DNA was extracted from sorted cells by first decrosslinking cells in a solution of 10 mM Tris pH 7.5 and 10 mg/mL Proteinase K (Roche) in PBS at 55°C for 24 hours ...
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bioRxiv - Immunology 2021Quote: ... Culture media was supplemented with 500 U/mL human IL-2 (Tecin from Roche, kindly provided by the NIH) starting on culture day 2 ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were hybridized with 30μg/mL of Digoxigenin (DIG) conjugated telomere probe (CCCTAACCCTAACCCTAACCCTAA-DIG, IDT) in DIG Easy Hyb buffer (Roche) overnight at 42°C ...
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bioRxiv - Immunology 2021Quote: ... the fibrin gel portions were cut into small pieces to be properly digested in an enzymatic solution of Collagenase/Dispase (0.5 mg/mL) (Roche) and DNase I (0.5 mg/mL ...
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bioRxiv - Microbiology 2021Quote: ... Epidermal and dermal sheets were prepared after incubation overnight at 4°C with 4 U/ml dispase II (Roche) in PBS by gently separating dermis and epidermis each as intact sheets (Rahn et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... Dissected lungs and kidneys were washed in DMEM/F12 and then incubated in 200ng/ml Collagenase/Dispase (Roche; 10269638001) in DMEM/F12 for 10 min (kidney ...
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bioRxiv - Neuroscience 2022Quote: ... DRGs from E13.5 embryos were dissected and placed in droplets of 2 mg/ml collagen (Roche Diagnostic, catalog#11179179001) together with COS1 aggregates transfected with either secreted Myc-Sema3A or control PAY1-GFP ...
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bioRxiv - Cancer Biology 2022Quote: Pancreatic tumors harvested from PKT mice were enzymatically digested using solution of 0.6 mg/ml of collagenase P (Roche), 0.8 mg/ml Collagenase V (Sigma Aldrich) ...
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bioRxiv - Developmental Biology 2022Quote: ... fins were incubated for 20 min at 28°C with vigorous shaking in a solution of Liberase DH Research Grade (0,05mg/ml in 1x PBS, Roche). Cell suspensions were passed through a 30 μm filter (CellTricks ...
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bioRxiv - Immunology 2022Quote: The lung tissue from infant and adult post-septic mice was digested with Liberase TL (0.2 mg / mL, Roche) and DNase I (0.5 mg / mL ...
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bioRxiv - Immunology 2019Quote: ... MLN from indicated groups of mice were dissected into small pieces and digested with freshly made enzyme mix comprised of RPMI-1640 containing 0.12 mg/ml Collagenase P (Roche), 0.4 mg/ml Dispase (Roche ...
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Caspase-8 Modulates Angiogenesis By Regulating A Cell Death Independent Pathway In Endothelial CellsbioRxiv - Developmental Biology 2019Quote: ... Tissue from Casp-8WT and Casp-8ECko pups from the same litter were pooled and digested in collagenase I/DNAse I (0.1mg/ml, Roche) at 37°C ...
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bioRxiv - Developmental Biology 2019Quote: ... whole E16.5 – E18.5 lungs were manually dissociated with a pestle in separate microcentrifuge tubes and then incubated with 2.5 mg/mL Collagenase A (Roche) and 20 ug/mL DNAse 1 (Sigma ...
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bioRxiv - Cancer Biology 2019Quote: ... coli cells containing the overexpressed RNF114 fusion protein were re-suspended in 80 mL of lysis buffer (50 mM Tris, pH 7.5, 150 mM NaCl, 2 Roche protease inhibitor tablets [without EDTA] ...
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bioRxiv - Developmental Biology 2019Quote: ... 1X Denhardt solution) containing 1μg/ml DIG labeled RNA probes (prepared with a DIG RNA labeling mix, Roche, 11277073910) for overnight at 65°C in a humid chamber ...
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bioRxiv - Genomics 2019Quote: ... Samples from the head skin of Prss53-/- and WT rabbits were homogenized and lysed in RIPA buffer supplemented with 2.5 μL/mL protease inhibitor cocktail (Roche) on ice for 30 min ...
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bioRxiv - Cell Biology 2019Quote: ... and the skin samples were cut into small pieces and incubated in 0.33 mg/ml of Liberase TM Research Grade (05401119001, Roche) at 37 °C for 30 min ...
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Genomic organization of the autonomous regulatory domain of eyeless locus in Drosophila melanogasterbioRxiv - Genetics 2021Quote: ... The fixed embryos were resuspended in 2.5 ml of ice-cold lysis buffer (10 mM Tris-pH-8, 10 mM NaCl, 0.2% NP40 with Roche protease inhibitor cocktail freshly added ...
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bioRxiv - Cell Biology 2021Quote: ... for 90 min at 37 °C and digested overnight at 37 °C in 1.5 mg/ml collagenase B solution (Roche). Then ...
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bioRxiv - Immunology 2021Quote: ... Lungs were dissected and digested for 30min at 37°C using RPMI media (2% FBS+ Pen/Strep, glutamine, 10mM HEPES) containing 0.5mg/mL Collagenase D (Roche). The digested fragments were passed through a 70μm cell strainer (Greiner bio-one ...
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bioRxiv - Microbiology 2021Quote: ... frozen pellets were thawed on ice in 50 mL conical flasks with pre-chilled lysis buffer (50 mM HEPES – KOH pH 7.6, 500 mM KCl, Roche Protease Inhibitors tablet (0.5 tablet/L of culture) ...