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Citations for Roche :
6401 - 6450 of 9754 citations for Monoisodecyl Phthalate 100 Ug Ml In Mtbe Unlabeled Mono 3 7 Dimethyl 1 Octyl Phthalate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Tissues were washed in HBSS and then dissociated with 1mg/ml of collagenase and dispase (Roche, Indianapolis, IN, USA) for 45 mins at 37 degrees ...
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bioRxiv - Developmental Biology 2023Quote: ... dissected tissues were dissociated for 30 mins at 37 °C in 200ul of LiberaseTM (Roche, 100ug/ml in PBS). 2 mL of cold PBS (without ca/Mg ...
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bioRxiv - Genetics 2023Quote: ... incubated the islets for 10 min at 37°C in 2 mL PBS with 4U Dispase I (Roche Diagnostics) / 2U DNase I (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Frozen pellets were thawed on ice and lysed with 20mls buffer per gram of pellet (50mM HEPES, 500mM NaCl, 0.5mM TCEP, 1U/ml DNase supplemented with Roche cOmplete™ ...
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bioRxiv - Biochemistry 2023Quote: ... prior to resuspension in 25 mL ice-cold water with another tablet of cOmplete™ ULTRA protease inhibitor (Roche), followed by centrifugation for 30 minutes at 15000×g and 4 °C ...
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bioRxiv - Immunology 2023Quote: ... The fixed cells were collected by scraping in 0.5 ml of ice cold 1x PBS supplemented with 1x cOmplete proteinase inhibitor (Roche) for U2OS and by centrifugation for SUDHL4 at 1000g for 5 min at 4°C ...
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bioRxiv - Genomics 2023Quote: The captured DNA was amplified for ten cycles using KAPA HiFi HotStart ReadyMix (6.25 ml, Roche, catalog number 7958935001) and purified in 1.8 x volume of Agencourt AMPure beads (Beckman Coulter ...
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bioRxiv - Developmental Biology 2023Quote: ... and thus we dechorionated Stage 24-25 embryos (or 2 to 2.5 dpf) pupfish by incubating them on 20 mg/ml Pronase (Roche) in water for 2.5 hours ...
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bioRxiv - Molecular Biology 2023Quote: Tissue disruption began physically using a pestle in 100μL of a digestion mix composed of 1mg/mL Liberase TM (5401119001, Roche), 1mg/mL Collagenase D (11088858001 ...
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bioRxiv - Immunology 2023Quote: ... The epidermis and dermis layers were separated after incubation at 37°C for 1h in 0.4 mg/mL dispase II (Roche). The epidermis was collected and then cut into small pieces using scalpels and incubated for 30 minutes with agitation at 37°C in RPMI containing 10% FCS and 0.5 mg/mL DNAse I ...
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bioRxiv - Immunology 2023Quote: ... The epidermis and dermis layers were separated after incubation at 4°C for 16h in 0.2 mg/mL dispase II (Roche). Epidermal layers were fixed in 4% Para-formaldehyde for 20 min at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... 75 μl of the sample were treated for 30 min at 50°C with 1.2 mg/ml Proteinase K (Roche) in Proteinase K Buffer (100 mM Tris-HCl ...
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bioRxiv - Physiology 2023Quote: Approximately 50 mg of adipose tissue was homogenized in 1 mL of lysis buffer (250 mM sucrose, 50 mM Tris Cl, pH 7.0, with protease inhibitor cocktail (11873580001, Roche)) ...
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bioRxiv - Immunology 2023Quote: ... Brain and spinal cord were cut into pieces and digested for 30 min at 37 °C with collagenase D (3.75 mg/ml; Roche) and DNase I (1 mg/ml ...
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bioRxiv - Systems Biology 2023Quote: ... The left lobes of the lung were then inflated with digestion solution containing 1.5 mg/mL of Collagenase A (Roche) and 0.4 mg/mL DNaseI (Roche ...
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MET functions in tumour progression and therapy resistance are repressed by intronic polyadenylationbioRxiv - Molecular Biology 2023Quote: ... and reconstituted at 0.5mg/ml in water and then further diluted to a 0.1 mg/ml stock solution in PBS (gibco) 0.1% BSA (Roche, #10735086001). Agarose was purchased from Invitrogen (#16500-500).
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bioRxiv - Developmental Biology 2023Quote: ... were placed in 250µL of ice-cold disassociation media (sterilized 1x PBS and 0.04% bovine serum albumin, 1.2 U/mL Dispase II (Roche 04942078001), 1 mg/mL Collagenase IV (Gibco 17104–019) ...
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Emergence of RNA-guided transcription factors via domestication of transposon-encoded TnpB nucleasesbioRxiv - Genetics 2023Quote: Flash-frozen cell pellets were resuspended in 1.2 ml RIP lysis buffer supplemented with cOmplete Protease Inhibitor Cocktail (Roche) and SUPERase•In RNase Inhibitor (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... cells were lysed in 100μL or 200μl of 2% SDS lysis buffer (2% SDS, 50mM Tris-HCl pH 7.5, 5mM EDTA, 15U/mL DnaseI (Roche), cOmplete mini EDTA-free protease inhibitor tablet (Roche ...
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bioRxiv - Biochemistry 2023Quote: ... then the pellet was resuspended in 40 ml binding buffer (50 mM NaPi, 300 mM NaCl, 5 mM imidazole, pH 7.5) containing protease inhibitor (Roche cOmplete EDTA-free protease inhibitor cocktail ...
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bioRxiv - Molecular Biology 2024Quote: ... at 10 mL/g of cell pellet) including protease inhibitors (complete protease inhibitor cocktail tablet, Roche Life Sciences, UK). The suspension was lysed using an Emulsiflex extruding homogenizer (Avestin ...
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bioRxiv - Bioengineering 2024Quote: ... The media was removed and 150 μL of serum free RPMI containing 0.25 mg/mL Liberase TL (Roche 054010120001) were added to the samples ...
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bioRxiv - Molecular Biology 2023Quote: ... flash-frozen pellets were first resuspended in 1.2 mL RIP lysis buffer supplemented with cOmplete Protease Inhibitor Cocktail (Roche) and SUPERase•In RNase Inhibitor (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... thrombus samples were obtained and homogenized with 0.3 ml of PBS (pH 7.2) containing Complete Protease Inhibitor Mixture (Roche Diagnostics). The homogenates were centrifuged at 12,000 g for 15 min ...
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bioRxiv - Cell Biology 2023Quote: ... 5 % glycerol and 0.5 mM TCEP supplemented with 5 Units/mL Benzonase and an EDTA-free protease inhibitor cocktail (Roche)) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were detached 24 h after transfection with Trypsin-EDTA (0.25% wt/vol) and re-plated onto 10 µg/ml fibronectin (Roche) pre-coated MatTek dishes with glass bottoms ...
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bioRxiv - Molecular Biology 2023Quote: SDGC-SEC-purified stress granule cores (strain JD1370) were incubated at 0.23 A260 units/mL with 2 or 4 units of RNase H (10786357001; Roche) in 40 μL of RNase H buffer (20 mM HEPES-KOH pH 8.0 ...
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bioRxiv - Biophysics 2024Quote: ... were resuspended in 20 mM Tris (pH 7.4) and 300 mM NaCl (50 mL volume) containing complete protease inhibitor cocktail (Roche) and lysed by soni- cation ...
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bioRxiv - Physiology 2024Quote: Islets were isolated by the Vanderbilt Islet and Pancreas Analysis (IPA) core by injecting 0.6 mg/ml collagenase P (Roche) into the pancreatic bile duct followed by a Histopaque-1077 (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... On the following day 10 µl MTT labeling reagent (5 mg/ml) of the Cell Proliferation Kit I (Roche) was added and cells were incubated at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... spores were then washed twice with sterile PBS and resuspended in a 1.0 mg/mL Proteinase K (Roche Laboratories) in 10mM Tris-HCl (Sigma-Aldrich) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Both forebrains and connective tissues were dissociated in the solution containing 2mg/ml Collagenase/Dispase (Roche, Basel, Switzerland; 10269638001) in Hanks’ Balanced Salt solution ...
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bioRxiv - Systems Biology 2024Quote: ... then resuspended in 1 mL ice-cold resuspension buffer (50 mM HEPES pH 7.2 with cOmplete Mini EDTA-free Protease Inhibitor Cocktail (Roche)) ...
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bioRxiv - Cancer Biology 2024Quote: The epidermis was separated from the dermal layer via digestion in Dispase II (final conc. 2.7 U/mL in PBS, Roche) for 30 min on ice ...
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bioRxiv - Immunology 2024Quote: ... kidneys were cut and digest for 30 min at 37°C with 0.5mg/ml collagenase D (Roche, Mannheim, Germany) and 0.05 mg/ml DNase I (Roche ...
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bioRxiv - Microbiology 2024Quote: ... Clarified supernatant was filtered through a 0.45 µM PES syringe filter and incubated with 10 mL of cOmplete His-Tag purification beads (Roche) for 1 h with gentle rocking ...
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bioRxiv - Immunology 2024Quote: Lung tissue was prepared by coarsely chopping the tissue followed by incubation in a 0.5 mg/mL solution of collagenase D (Roche) and 500 Units of DNase (Sigma Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... Cells were lysed with 30 µL lysis buffer (final concentration: 50 μg/ml lysozyme, 0.8% NP-40, 1x protease inhibitor (Roche), 250 U/ml benzonase and 1 mM MgCl2 in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mM MgCl2, 1 mM EGTA, 0.1% [v/v] NP-40, 1 mM DTT, 5% [v/v] glycerol and Roche Complete Protease Inhibitors) and homogenized using a high-performance disperser (Fisher) ...
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bioRxiv - Cell Biology 2020Quote: HEK293T cells were lysed in 1% DMNG buffer (150mM NaCl, 50mM Tris-HCl pH 7.5, 5mM MgCl2, 1% DMNG, protease inhibitors (Roche diagnostics, EDTA free) for 90 min ...
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bioRxiv - Biochemistry 2019Quote: ... from 4L of bacterial culture was resuspended in 30 mL lysis buffer (50 mM Tris-HCl pH 8.3, 1 mM EDTA, 150 mM NaCl, 10% glycerol, 5 mM DTT, 1 mM PMSF, and Roche protease inhibitor cocktail) and sonicated on ice ...
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bioRxiv - Molecular Biology 2019Quote: ... The enzyme digestion was carried out by digestion buffer (calcium-free Tyrode buffer supplemented with hyaluronidase [Worthington, 0.1 mg·ml-1, LS005477] and collagenase type B [Roche, 0.35 U·ml-1, 11088807001]). The left ventricle was collected after 10 minutes’ digestion and cut into small pieces ...
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bioRxiv - Biophysics 2019Quote: ... Cells were then incubated for 1 h at 4° C in PBS + 1% BSA with a 1:1000 dilution of rat anti-HA monoclonal antibody (Roche; Basel, Switzerland) and washed 5×10 min on ice with PBS + 1% BSA ...
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bioRxiv - Cell Biology 2019Quote: ... 2 mM MgCl2, 1 mM EGTA, 0.1% [v/v] NP-40, 1 mM DTT, 5% [v/v] glycerol and Roche Complete Protease Inhibitors) and homogenized using a high-performance disperser (Fisher) ...
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bioRxiv - Plant Biology 2021Quote: ... Three volumes of lysis buffer (50 mM Tris-Cl pH 7.4, 150 mM KCl, 1% Triton-X100, Protease inhibitor 1 X [Roche], NEM 20 μM) was added to 2 g of powdered tissue ...
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bioRxiv - Cell Biology 2021Quote: ... 1.5 mM MgCl2, 1 mm EDTA, 0.5 mM DTT, 50 mM NaF, 50 mM sodium pyrophosphate, 1 mM Na3VO4 and Roche protease inhibitor cocktail) by glass bead lysis ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were collected via centrifugation and lysed in lysis buffer (1.5M KCl, 20 mM Tris pH 8.0, 20 mM Imidazole pH 8, 1 mM DTT, 1 tablet of Roche protease inhibitor cocktail). Sonication was performed to further lyse cells and to shear DNA ...
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bioRxiv - Molecular Biology 2022Quote: ... The adhered cells were then gently washed with cold PBS and lysed in 1% NP-40 lysis buffer (50mM Tris-HCl pH 8.0, 150 mM sodium chloride, 1% NP-40, Roche Complete protease inhibitor). The lysate was then cleared by centrifugation at 17,000 x g for 10 min at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... 0.2% NP-40, 10% glycerol, 1 mM EDTA, 1 mM PMSF, 20 µM MG132, 5 mM DTT and Roche protease inhibitor #5892953001), and incubated for 1 h on a rotating wheel ...
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bioRxiv - Plant Biology 2021Quote: Frozen inflorescence tissue was ground with liquid nitrogen and resuspended in lysis buffer (50 mM Tris pH 8, 150 mM NaCl, 5 mM MgCl2, 10% glycerol, 1% IGEPAL, 0.5 mM DTT, 1 mM PMSF, 1X Roche protease inhibitor cocktail) and homogenized with mixing for 15 minutes at 4°C ...