Labshake search
Citations for Roche :
5951 - 6000 of 7005 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 1 μg of RNA was converted to cDNA using an oligo dT primer and the Transcriptor First Strand cDNA Synthesis kit (Roche Molecular Systems). qPCR was performed using PowerUp SYBR Green (Applied Biosystems Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... Total RNA was then provided to the Functional Genomics Laboratory at UC Berkeley where RNA-seq libraries were prepared by Oligo dT enrichment followed by a stranded Illumina library prep protocol with the KAPA mRNA HyperPrep kit (Kapa Biosystems, KK8580). Libraries were checked for quality on an AATI Fragment analyzer (Agilent ...
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bioRxiv - Pathology 2022Quote: ... Extraction of total nucleic acid was performed using the MagNA Pure 96 DNA and Viral NA Small Volume Kit in the MagNA Pure 96 system (Roche, Penzberg, Germany), and eluted in a volume of 50μl Roche Tris-HCl elution buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA was diluted to a final concentration of 5 ng/μl and RT–qPCR was performed with KAPA SYBR® FAST qPCR kits (KAPA Biosystems) on a C1000 thermal cycler ...
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bioRxiv - Genomics 2020Quote: ... Whole transcriptome libraries were constructed using the KAPA Stranded mRNA-seq library kit (Roche Sequencing and Life Science, Kapa Biosystems, Wilmington, MA) from 250ng of total input RNA with the Beckman Coulter Biomek FXP automation system (Beckman Coulter ...
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bioRxiv - Plant Biology 2020Quote: ... Blots were hybridized with a digoxygenin (DIG)-labeled ZmPEPC promoter-specific probe synthesized using primers (Fwd 5’-TCCCGAGTTCCTAACCACAG; and Rev 5’-GTGGCTGAGGCTTCTTTTTG) and the PCR DIG Probe Synthesis Kit (Roche Diagnostics, Switzerland). The signals were detected by CDP-Star (Roche Diagnostics ...
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bioRxiv - Synthetic Biology 2020Quote: ... taiwanensis VLB120 was used as the template for the PCR and was isolated using the High Pure PCR Template Preparation Kit (Hoffmann-La-Roche, Basel, Switzerland). Plasmids were constructed by Gibson assembly using the NEBuilder Hifi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Cell Biology 2020Quote: ... Mip1α and housekeeping gene β-actin was evaluated by quantitative RT-PCR using the LightCycler 480 SYBR Green 1 Master kit and LightCycler 480 II (Roche, Indianapolis, IN) and oligos ...
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bioRxiv - Microbiology 2020Quote: ... and then the pooled libraries were diluted for accurate qPCR quantification using KAPA Library Quantification Kit for Illumina platforms (KAPA Biosystems, MA). The libraries pools were adjusted to a final concentration of 11.5 pM (for V2 kits ...
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bioRxiv - Genetics 2021Quote: ... 500 ng total RNA was used for generating strand-specific RNA-seq libraries using the KAPA Stranded RNA-Seq Kit with RiboErase (Kapa Biosystems, MA). RNA-seq libraries were sequenced on an Illumina NextSeq 500 platform with paired-end 75 bp read length.
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bioRxiv - Immunology 2021Quote: ... 5 μl of the resulting cDNA-containing reverse transcription mixes were then used as templates for 25 μL PCR reactions using the KAPA HiFi PCR kit with GC buffer (Roche Diagnostics, #07958846001) and the following thermal cycling conditions ...
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bioRxiv - Genetics 2020Quote: ... A small portion of the vitelline glands from the posterior part of each fluke was used for DNA extraction using the High Pure DNA Extraction Kit (Roche, Mannheim, Germany) following the manufacturer’s protocols ...
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bioRxiv - Genetics 2021Quote: The bone libraries were prepared in a dedicated low copy DNA laboratory using the KAPA Hyper Prep Kit (Roche Sequencing, Wilmington, MA). Library preparation followed the manufacturer’s recommendations using 15 µM 8-bp dual-indexed adapters ...
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bioRxiv - Genetics 2021Quote: ... The T7 promoter was added to the 5’ and 3’ ends to synthesize the sense and anti-sense probes using the DIG RNA Labeling Kit (Roche, Cat# 11175025910). In situ hybridization was performed as previously described [65,66] with minor modifications ...
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bioRxiv - Physiology 2020Quote: ... Tissues were homogenised in assay buffer provided in the kit with the addition of 5 μL of protease inhibitor cocktail (Roche, Basel Switzerland) and centrifuged at 800 × g for 10 minutes at 4°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Library preparation was carried out using KAPA Hyper Prep and HiFi HS Library Amplification kits (F. Hoffmann-La Roche AG, Basel, Switzerland) and with iTru i5 and i7 dual-indexing primers (BadDNA ...
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bioRxiv - Cell Biology 2020Quote: ... Southern blotting was performed as instructed in the instruction manual for the DIG-High Prime DNA Labeling and Detection Starter Kit II (Roche Cat. 11585614910).
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bioRxiv - Evolutionary Biology 2021Quote: ... and the product was used for the second round PCR using the primers F and R-T7 for generating antisense probe and primers R and F-T7 for generating sense probe used in ISH assays with the DIG RNA Labeling Kit (Roche, Mannheim, Germany),the primers were shown in table 1 ...
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bioRxiv - Developmental Biology 2021Quote: ... Sequencing libraries were prepared starting from 500 ng for each DNA sample with the KAPA HyperPrep Kit (Hoffman-La Roche, Basel, Switzerland) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Qualitative and quantitative analysis of the NGS library preparation process was conducted using a chip-based electrophoresis and libraries were quantified using a qPCR-based quantification kit (Roche, Cat. # KK4824). Sequencing was performed using Illumina NextSeq500 platform (Illumina Inc) ...
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bioRxiv - Molecular Biology 2021Quote: RNA template (500 ng) was used for one-step RT-PCR amplification protocol (SYBR Green I RNA amplification kit, Roche, Basel, Switzerland). Primers were designed using the LC Primer/Probe design software (Roche ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The primary antibody was either visualized with the multimer-technology based UltraView Universal DAB Detection kit (Ventana® BenchMark® XT; Roche) or a fluorochrome-labeled secondary antibody (Alexa-conjugated ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA probes were synthesized by in vitro transcription under the drive of SP6 promoter with the DIG RNA Labeling Kit (Roche, Mannheim, Germany); Then ...
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bioRxiv - Cancer Biology 2020Quote: ... The libraries were quality controlled on an Agilent 2100 Bioanalyzer with the DNA 7500 assay for size and the concentration was estimated using quantitative PCR with the KAPA Library Quantification Kit Illumina® Platforms (Roche Kapa Biosystems). To obtain sufficient amount of libraries for sequencing it was necessary for the low input libraries (0,1 - 0,2 ug ...
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bioRxiv - Microbiology 2020Quote: ... and prepared for shotgun metagenomic sequencing using the Kapa Hyperprep Plus kit with 3 rounds of PCR amplification (Kapa Biosystems, Wilmington, MA), and sequenced to an average depth of 7 gbp per fraction on the Illumina NovaSeq (Illumina ...
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bioRxiv - Genomics 2021Quote: ... and then either the remaining components of the PowerSoil Pro kit or DNA purification beads from Kapa Biosciences (Roche, Burgess Hill, UK) and either the MPBio SuperFastPrep-2 (MPbio ...
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bioRxiv - Cancer Biology 2021Quote: ... Femurs were then decalcified and paraffin sections (5-μm thickness) were stained with hematoxylin and eosin or reticulin (Reticulum II Staining Kit, Roche, Tucson, AZ) to assess fibrosis ...
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bioRxiv - Microbiology 2020Quote: ... Total nucleic acid was extracted from the lysate using the Roche MagNA Pure Compact Nucleic Acid Isolation Kit I and the MagNA Pure Compact instrument (Roche, Indianapolis, Indiana). Dual-indexed sequencing libraries were prepared from the lysates using Nextera XT DNA Library Preparation Kit (Illumina FC-131-1096) ...
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bioRxiv - Microbiology 2020Quote: Each 300 μL of GEA supernatant sample was extracted using the High Pure PCR Template Preparation Kit (Roche Life Science, Mannheim, Germany), a kit based on silica-membrane spin columns technology ...
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bioRxiv - Molecular Biology 2021Quote: ... the probe for RNA FISH was prepared with 2 µg of BAC RP183A17 using Nick translation kit (10976776001) purchased from Roche (Basel, Switzerland). Salmon sperm DNA and cot1 DNA were added to the probe to mask the repetitive sequence ...
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bioRxiv - Immunology 2020Quote: ... CD206 and CD64 was performed by Fast Start DNA Masterplus SYBR Green I kit on the Light Cycler 480 (Roche, Applied Science). Relative RNA expression levels were normalized to the geometric mean of two reference genes RPL37A and ACTB ...
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bioRxiv - Immunology 2020Quote: ... cultured in vitro at 30 °C and at 37 °C in EMJH medium was also extracted using a High Pure RNA Isolation kit (Roche Applied Science) following the manufacturer’s recommendations ...
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bioRxiv - Genetics 2020Quote: ... The membrane was hybridized using digoxigenin-labeled DNA probes (S4 Table) for mPSP based on the DIG-High Prime DNA Labeling and Detection Starter Kit II protocol (Roche, Mannhein, Germany). For western blotting ...
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bioRxiv - Microbiology 2022Quote: ... Samples were transferred to a high pure extender assembly from the High Pure Viral Nucleic Acid Large Volume kit (Roche, Mannheim, Germany) and centrifuged for 8 min with 1,500 rpm at room temperature ...
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bioRxiv - Genetics 2022Quote: ... to evaluate library size and adapter dimer presence before the library concentration was determined via quantitative real time PCR using the KAPA library quantification kits (KK4824, Roche, Basel, Switzerland) on the QuantStudio7 or ViiA7 (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... The libraries were quantified by fluorometry on a Qubit instrument (LifeTechnologies, Carlsbad, CA) and by qPCR with a Kapa Library Quant kit (Kapa Biosystems-Roche) prior to sequencing ...
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bioRxiv - Bioengineering 2022Quote: ... Acetate concentration in the supernatant was measured using the Cedex Acetate V2 Bio HT kit on a Cedex HT system (Roche Custom Biotech). OD600 was measured using a Ultrospec 2100 Spectrophotometer (Amersham Biosciences).
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bioRxiv - Developmental Biology 2022Quote: ... Antisense and sense RNA probes of TaDOF5.6 and TaDOF3.4 genes were synthesized using a DIG RNA Labeling Kit (SP6/T7) (Roche, Cat No. 11175025910, USA). The oligonucleotide primers for in situ hybridization are listed in Table S28.
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bioRxiv - Genomics 2022Quote: ... The resulting libraries were purified following the Nextera XT protocol (0.6× ratio) and quantified by qPCR using Kapa Library Quantification kit (Kapa Biosystems, Cat. KK4824). Four plates were pooled together on a NextSeq 550 high-output flow cell or NextSeq 2000 P2-100 flow cell with 26 bp in read 1 and 50 bp in read 2 ...
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bioRxiv - Genomics 2022Quote: ... The amplification of 16S rRNA genes was conducted using the KAPA2G™ Robust HotStart ReadyMix PCR Kit (Kapa Biosystems, Wilmington, MA, USA) in a total volume of 25□μl ...
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bioRxiv - Plant Biology 2022Quote: ... The enriched biotinylated DNA fragments were subjected to library construction on beads using the KAPA HyperPrep Kit for Illumina (Roche, Basel, Switzerland) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: EMSA DNA fragments were labelled with digoxigenin (DIG) following the manufactureŕs instructions (DIG Gel Shift Kit 2nd Generation; Roche Diagnostics, IN, USA) and 30 fmol of labelled DNA was incubated with different amounts of purified HaWRI1_DBD recombinant protein (0-1000 ng ...
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bioRxiv - Plant Biology 2022Quote: ... The libraries were assessed for size and purity using the Agilent 2100 Bioanalyzer and quantitated by qPCR using a KAPA Biosystems Library Quantitation kit (Roche, Pleasanton, CA). Libraries were normalized ...
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bioRxiv - Pathology 2022Quote: RNA was reverse-transcribed and amplified using the TaqMan Fast Virus 1-Step Master Mix RT-qPCR kit (LifeTechnologies, Carlsbad, CA) on the LightCycler 480 or LC96 instrument (Roche, Indianapolis, IN), and quantified by interpolation onto a standard curve made up of serial tenfold dilutions of in vitro transcribed RNA ...
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bioRxiv - Immunology 2022Quote: ... cells were collected 24 h after incubation with 40 ng/ml IL-10 following the protocol of the High Pure RNA Isolation Kit (Roche, Basel, Switzerland).
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bioRxiv - Microbiology 2022Quote: ... then guide sequences were amplified from gDNA and the plasmid pool by nested PCR using KAPA HIFI Hotstart PCR kit (Kapa Biosystems, KK2501), as previously described in (Young et al. ...
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bioRxiv - Physiology 2022Quote: ... The PCR products of target genes were purified and used as templates for probe preparation using DIG or fluorescein RNA labeling kits (Roche, Basel, Switzerland). The pituitary sections were hybridized with the antisense RNA probes for fshb and lhb as we previously reported [52] ...
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bioRxiv - Cell Biology 2022Quote: cDNA was synthesized from 100–500 ng of total RNA in 10 µl reactions with a Transcriptor First Strand cDNA Synthesis Kit (Roche, Basel, Switzerland) according to the manufacturer’s protocol using random hexamer primers and an MJ Mini Personal thermal cycler (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... cDNAs and final libraires were run on an Agilent 4200 TapeStation to check for quality and were quantified using KAPA Library Quantification Kit (Roche, Basel, Switzerland).
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bioRxiv - Microbiology 2024Quote: ... while supernatants from enriched soil/water samples and rectal swabs were extracted using the High Pure PCR Template Preparation kit (Roche, Mannheim, Germany) according to the manufacturer’s instructions ...