Labshake search
Citations for Roche :
5751 - 5800 of 7005 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... One μg of total RNA was used for polyA-RNA isolation and library preparation using the KAPA mRNA Hyperprep kit (Roche, KK8580) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Quantification of specific gene transcripts was achieved through droplet digital PCR (ddPCR) using the Digital LightCycler 5× RNA Master Kit (Roche, swiss) with specific primers and probes ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing libraries were produced by conducting seven PCR cycles using a KAPA LTP library preparation kit (Roche Sequencing and Life Science) with NEBNext multiplex oligos for Illumina (New England Biolabs) ...
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bioRxiv - Microbiology 2024Quote: ... DNA_P4 was MagNA Pure 96 DNA and Viral NA Small Volume Kit on the MagNA Pure 96 Instrument (Roche, Basel, Switzerland) with proteinase K pre-treatment step and standard buffers using the DNA Blood ds SV Protocol optimized for double stranded DNA and Next Generation Sequencing (Table 1) ...
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bioRxiv - Genomics 2024Quote: ... The duplicate reactions were pooled and the libraires were enriched for viewpoints of interest in a double capture procedure using the KAPA Hyper Capture Reagent Kit (Roche, 9075828001). A total of 8 µg of indexed sample per biological replicate was used as input for the first capture ...
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bioRxiv - Immunology 2024Quote: ... Poly(A)-enriched cDNA libraries were generated using the Illumina TruSeq sample preparation kits and quality and quantity verified using the bioanalyzer and qPCR (KAPA Biosystems). Using dual-index barcoding methods to reduce index hopping ...
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bioRxiv - Immunology 2024Quote: ... Indexed libraries that meet appropriate cut-offs for both are quantified by qRT–PCR using a commercially available kit (KAPA Biosystems). Insert size distribution was determined with the LabChip GX or Agilent Bioanalyzer ...
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bioRxiv - Immunology 2024Quote: ... PN-1000250).The concentration of each library was accurately determined through qPCR using the KAPA library Quantification Kit according to the manufacturer’s protocol (KAPA Biosystems/Roche) to achieve the desired cluster counts for the Illumina NovaSeq6000 instrument ...
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bioRxiv - Physiology 2024Quote: ... Indexed libraries that met appropriate cut-offs for both quantity and quality were quantified by qRT-PCR using a commercially available kit (KAPA Biosystems) and insert size distribution determined with the LabChip GX or Agilent Bioanalyzer ...
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bioRxiv - Immunology 2024Quote: ... The final libraries were analyzed using the Agilent bioanalyzer and the libraries were quantified using the Kapa SYBR FAST Universal qPCR kit for Illumina sequencing (Kapa Biosystems). The individual libraries were diluted to 6 nM and 2 uL of each added to a library pool ...
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bioRxiv - Immunology 2024Quote: ... cDNA was reverse transcribed using a HiScript III 1st Strand cDNA Synthesis Kit (Vazyme) and qPCR was performed using ChamQ SYBR® qPCR Master Mix (Vazyme) with a LightCycler 96 instrument (Roche). The relative mRNA expression was calculated by the value of 2-ΔCt normalized to 18S ...
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bioRxiv - Molecular Biology 2024Quote: ... Real-time PCR was performed to assess the concentration of target rRNA using SYBR Green Master Mix kit (Roche, Basel, Switzerland) in accordance with the manufacturer’s recommendations on LightCycler® 96 Real-Time PCR System (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries for RNAseq were prepared for each sample with a KAPA stranded mRNA-seq kit as per manufacturer’s recommendations (Roche, Indianapolis, IN); briefly ...
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bioRxiv - Neuroscience 2024Quote: ... Insert sizes were evaluated via Caliper GX (Perkin-Elmer, USA) and libraries quantitated with Kapa SYBR FAST Universal kit (Roche, USA). Sequencing was performed on the Illumina NovaSeq 6000 platform to generate 100M paired reads per sample at 150 nucleotide read lengths ...
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bioRxiv - Genomics 2024Quote: ... Final library quality was assayed using instrumentation described above as well as qPCR using the Kapa Library quantification kit for Illumina (Roche Inc.), before sequencing with other libraries on a quarter Illumina Novaseq S4 flowcell with 2*150 bp paired-end mode at the Norwegian Sequencing Centre.
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bioRxiv - Microbiology 2024Quote: ... mixed with 15 μL of a PCR mix with 1X LightCycler FastStart DNA Master Hybridisation Probes kit (Roche diagnostics, Mannheim, Germany), 0.5 U of UDG (Roche Diagnostics ...
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bioRxiv - Cancer Biology 2024Quote: ... The number of PCR cycle used for library amplification was determined by KAPA library quantification kit for Illumina platform (KAPA biosystems). Each Hi-C library was sequenced to obtain 500M reads PE100 with biological duplicates to achieve a total of 1 B reads for each condition.
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bioRxiv - Microbiology 2024Quote: ... library preparation for metagenomic analysis was conducted using the KAPA Hyper PCR-free library preparation kit (Kapa Biosystems, Roche, Basel, Switzerland), following the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2024Quote: ... library preparation for metagenomic analysis was conducted using the KAPA Hyper PCR-free library preparation kit (Kapa Biosystems, Roche, Basel, Switzerland), following the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2024Quote: ... all extracted genomes were used as the PCR templates and the sgRNA-coding sequences with iBAR were amplified using KAPA HiFi HotStart ReadyMix PCR kit (Roche, #KK2631). The DNA amplification was performed under the following condition ...
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bioRxiv - Molecular Biology 2024Quote: ... and an equimolecular pool of libraries was titrated by quantitative PCR using the “Kapa-SYBR FAST qPCR kit for LightCycler480” (Kapa BioSystems) and a reference standard for quantification ...
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bioRxiv - Microbiology 2024Quote: ... Plasma creatinine was measured in freshly thawed plasma samples using the CREP2 kit (Catalog Number 03263991190) on a Cobas 311 analyser (Roche, USA). Assays were performed by a commercial laboratory in the UK ...
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bioRxiv - Immunology 2024Quote: ... The concentration of each library was accurately determined through qPCR utilizing the KAPA library Quantification Kit according to the manufacturer’s protocol (KAPA Biosystems/Roche) to produce cluster counts appropriate for the Illumina NovaSeq6000 instrument ...
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bioRxiv - Molecular Biology 2024Quote: ... activity was measured in a kinetic assay,39 using a commercial kit (Sentinel Diagnostics 17234H) adapted for use on either a Cobas Fara or Mira analyser (Roche Diagnostics). Intra-assay and inter-assay precision were CV < 4% and CV < 8% respectively.
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bioRxiv - Plant Biology 2024Quote: ... In total of 1 μg total RNA was used for first-strand cDNA synthesis using Transcriptor First Strand cDNA synthesis kit (Roche, Switzerland) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... We purified the AAV genomes from the envelope-AAV samples using the High Pure Viral Nucleic Acid Kit (Roche, Indianapolis, IN). For all AAV-based vectors ...
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bioRxiv - Cell Biology 2024Quote: ... Programmed cell death was visualized with TUNEL assay according to manufacturer’s instructions for TUNEL TMR red kit (11767291910, 5% TUNEL enzyme; Roche Diagnostic Corp).
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bioRxiv - Genomics 2024Quote: ... cDNA was synthesized from 100 ng of total RNA per sample using the Transcriptor First Strand cDNA Synthesis Kit (Roche, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... PCR amplification of libraries equivalent to 4 pg of DNA was performed with the KAPA HiFi Uracil+ kit (Roche, Cat No.KK2802) and primers having a Y-adaptor sequence with a phosphorothioate bond(* ...
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bioRxiv - Genomics 2024Quote: ... 10-30ng of the immunoprecipitated material was used for ChIP-seq library preparation using the KAPA Hyper prep kit (KAPA Biosystems) and applying 7-8 cycles of amplification ...
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Developmental functions of rapgef1b in neural crest specification and presomitic mesoderm patterningbioRxiv - Developmental Biology 2024Quote: ... The sequence-verified plasmid was linearised using Nco1 and antisense digoxigenin (DIG)-labeled riboprobes were synthesized using the DIG RNA labeling kit (Roche #11175025910). (Iyer et al ...
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bioRxiv - Cancer Biology 2024Quote: ... and 0.6 to 5 nanograms of enriched DNA was used for library preparation using the Kapa HyperPrep Kit (Roche, Cat. # 07962347001) following EpiCypher’s parameters for indexing PCR and library amplification as described in the CUT&RUN Library Prep Manual ...
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bioRxiv - Cell Biology 2024Quote: ... Indexed libraries that meet appropriate cut-offs for both are quantified by qRT-PCR using a commercially available kit (KAPA Biosystems) and insert size distribution determined with the Lab Chip GX or Agilent Bioanalyzer ...
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bioRxiv - Cell Biology 2024Quote: ... and 500 ng RNA was used as input for polyadenylate-tailed RNA enrichment and library preparation using the KAPA mRNA HyperPrep Kit (Kapa Biosystems). Assessment of library quality and quantification was carried out using Bioanalyzer High Sensitivity DNA analysis and the Qubit High Sensitivity fluorometry assay according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... PCR was performed with the single stranded oligo pool used as a template by forward and reverse primers (complementary to underlined sequences indicated above) with KAPA HiFi HotStart ReadyMix PCR Kit (50-196-5217; Roche Diagnostics) and purified with NucleoSpin Gel and PCR Clean-Up (740609 ...
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bioRxiv - Cancer Biology 2024Quote: ... Libraries were quantified for paired-end sequencing (101bp x 101bp) on the Illumina NovaSeq 6000 using Kapa Library Quantification Kits (Roche KK4824).
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bioRxiv - Developmental Biology 2024Quote: ... Hox probes and Hoxdn C-terminal probes were produced using PCR and transcribed using the DIG-RNA Labelling Kit (Roche, #11175025910) with T7 polymerase ...
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bioRxiv - Developmental Biology 2024Quote: ... The sequence-verified plasmid was linearised using Nco1 and antisense digoxigenin (DIG)--labeled riboprobes were synthesized using the DIG RNA labeling kit (Roche #11175025910). (51).
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed in ice-cold FACS buffer (2% FBS, (0.5 ug/ml) DNase I (Roche), 2 mM EDTA in PBS) ...
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bioRxiv - Immunology 2021Quote: Splenocytes lysates were prepared using Cell Lysis Buffer (#9803, CellSignaling Technology) supplemented with protease inhibitors (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... After dissection cell suspensions were prepared by vortexed with 1.5μl 1x Liberase TM (Roche, LIBTM-RO) in 500 μl cold PBS ...
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bioRxiv - Neuroscience 2021Quote: Cell lysates in 1% sarcosyl were digested with 75 µg/ml proteinase K (PK; Roche, Switzerland) for 1 h at 37 °C and subsequently denatured in an equal volume of 2x sample loading buffer at 110 °C ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed three times with 1X PBS supplemented with protease inhibitor cocktail without EDTA (Roche) and solubilized in Native PAGE sample buffer supplemented with 10% glycerol ...
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bioRxiv - Immunology 2021Quote: ... Viral plasmids were transduced in HEK-293T cells using X-tremeGENE HP DNA transfection reagent (Roche). After the addition of X-tremeGENE HP to the viral plasmids the solution was mixed in 100 µL of DMEM medium without serum or antibiotics ...
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bioRxiv - Neuroscience 2020Quote: ... Treated or untreated transfected HEK cells were lysed in cold IP buffer with protease inhibitors (Roche), and centrifuged for 10 minutes and 5,000 rpm at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... and a single-cell suspension was prepared by digesting the tissue in collagenase type A (Roche) and 25 μg/ml DNase (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed twice in ice-cold phosphate buffered saline containing protease inhibitor cocktail tablets (Roche). Cells were scraped into conical tubes ...
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bioRxiv - Bioengineering 2021Quote: HEK293T cells were lysed in RIPA lysis buffer (Applygen) supplemented with complete protease inhibitor cocktail (Roche) 48 h after transfection ...
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bioRxiv - Cell Biology 2021Quote: ... after translations the SP cells were incubated on ice with 10 µg/ml proteinase K (Roche) in the presence of 1 mM CaCl2 for 25 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were harvested and resuspended in 2 ml of 1X PBS containing protease inhibitor cocktail (Roche) and 500μl of resuspended cells was taken as a control for uncrosslinked sample (referred to as -DSG in the text) ...