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Citations for Roche :
6051 - 6100 of 7005 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Final cDNA libraries were checked for quality by TapeStation and qPCR (Kapa’s library quantification kit for Illumina Sequencing platforms, catalogue# KK4835, Kapa Biosystems, Wilmington MA). Samples were clustered and sequenced using NovaSeq S2 reagents (NovaSeq S2 Run ...
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bioRxiv - Plant Biology 2024Quote: ... The relative expression of genes in leaves was determined by reverse transcription quantitative PCR performed on an Applied Biosystems StepOne Real-Time PCR System with KAPA SYBR® FAST qPCR Master Mix (2X) Kit (Kapa Biosystems) according to the protocol of the manufacturer ...
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bioRxiv - Plant Biology 2024Quote: ... DNA fragments were then subjected to a next-generation sequencing library preparation procedure consisting of end repair and Illumina adaptor ligation using the KAPA HTP kit (Roche, Basel, Switzerland). Individual index sequences were added to each library ...
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bioRxiv - Molecular Biology 2024Quote: ... was annealed with Coccus-R primer (5′–ACG– TCA–GAA–TCG–CTG–C–3′) and analyzed using FastStart Essential DNA Green Master kit (Roche, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... DNA from the EM1 virus was labelled to be hybridized against the membrane using the DIG High Prime DNA Labelling and Detection Starter Kit II (Roche, Mannheim, Germany), following manufacturer’s instructions.
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bioRxiv - Pathology 2024Quote: ... The mutants were further confirmed by Southern blot assay using DIG-High Prime DNA labeling and detection starter kit I (Cat. no. 11745832910, Roche, Mannheim, Germany). For complementation ...
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bioRxiv - Plant Biology 2024Quote: ... A final DNA amplification step was carried out using the KAPA HiFi Real-Time PCR Library Amplification Kit (Kapa Biosystems, Boston, USA). Amp-DAP DNA libraries were purified with Agencourt AMPure XP beads (Beckman Coulter ...
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bioRxiv - Immunology 2024Quote: ... 7.4-10 ng of amplified cDNA was used to prepare libraries with the KAPA Hyper Prep Kit (Kapa Biosystems cat. no. KK8504) using 8 cycles of PCR ...
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bioRxiv - Neuroscience 2024Quote: ... DNA was isolated from tail clips taken from neonatal mice during sacrifice using the Kapa Express Extract Kit (Kapa Biosystems, Cat. #KK7100) with one minor modification – sample lysis was assisted via physical homogenization using a 1.5 mL tube plastic pestle ...
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bioRxiv - Evolutionary Biology 2024Quote: A PCR free Illumina library (based on the same HMW extraction) was prepared using the Kapa Hyper Prep Kit (Roche, Basel, Switzerland), following manufacturer instructions ...
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bioRxiv - Neuroscience 2024Quote: ... For bulk RNA-seq strand-specific polyA-enriched RNA libraries were prepared using the KAPA Stranded mRNA Sample Preparation Kit according to the manufacturer’s protocol (Kapa Biosystems, MA, USA). Briefly ...
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bioRxiv - Plant Biology 2024Quote: ... The labeled RNA probes were synthesized using in vitro transcription in the presence of Digoxigenin-label by RNA polymerases T7 or SP6 provided with DIG RNA labeling kit (Roche Diagnostics, Switzerland). The tissue sections of 10 µm thickness were sectioned by MICROM HM325 rotary microtome followed by deparaffinization with 100% lemosol for 10 mins and hydrated in graded ethanol series ...
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bioRxiv - Neuroscience 2024Quote: ... Libraries were then quantified at the Genome Quebec Innovation Centre (Montreal, Quebec) using a KAPA Library Quantification kit (Kapa Biosystems, United States), and average fragment size was determined using a LabChip GX (PerkinElmer ...
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bioRxiv - Genetics 2024Quote: Paired-end sequencing libraries for QTL-Seq analysis were prepared with >1 µg of DNA using a KAPA HyperPrep PCR-free kit (Roche, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... The cDNA synthesis was performed via reverse transcription using TaqMan™ Reverse Transcription Reagents Kit (Applied Biosystems, Roche Molecular System, Darmstadt, Germany).
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bioRxiv - Bioengineering 2024Quote: The libraries were quantified using KAPA Library Quantificatoin kits for Illumina Sequecing platforms according to the qPCR Quantification Protocol Guide (KAPA BIOSYSTEMS, #KK4854) and qualified using the TapeStation D1000 ScreenTape (Agilent Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... Details of these procedures were described in our previous study with a modification where CD8 was visualized by a DISCOVERY Yellow detection kit (Roche #760-228) after being stained with the primary and secondary antibodies.(16 ...
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bioRxiv - Genetics 2024Quote: ... was performed according to standard protocol (Thisse and Thisse, 2008) on embryos at 48 hours post fertilization (hpf) using DIG RNA Labeling Kits (Roche, Basel, Switzerland). Anti-sense and sense RNA probes were synthesized using T7 or T3 RNA polymerases (Promega ...
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bioRxiv - Evolutionary Biology 2024Quote: ... sheared to a target fragment size of 400–600 bp with a QSonica Q800R sonicator (Qsonica, Newtown, CT, U.S.A.) into a genomic DNA library preparation protocol (KAPA HyperPrep Kit, KAPA Biosystems, Wilmington, MA, U.S.A.) for targeted enrichment of UCEs following Faircloth et al ...
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bioRxiv - Microbiology 2020Quote: ... Protein lysates were obtained by lysing cells in 0.1% SDS and protease inhibitor cocktail tablets (Roche #04693124001). Cell lysates were separated by SDS-PAGE (Bio-Rad 4-20% Mini-PROTEAN TGX Precast Gel ...
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bioRxiv - Molecular Biology 2020Quote: Proliferative capacity of cells was analysed using 5-bromo-2’-deoxy-uridine (BrdU) labelling (Roche, Basel, Switzerland). Cells seeded on coverslips were pulsed with 10 μM BrdU for 60 min at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Whole cell extracts were prepared by lysis in RIPA buffer supplemented with protease and phosphatase inhibitors (Roche). Protein concentration was quantified by BCA assay (Thermo Fisher ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were lysed with lysis buffer (500 μL) containing the cOmplete protease inhibitor cocktail (Roche, cat#11697498001). The biotinylated surface proteins were excluded with NeutrAvidin agarose gel (Pierce ...
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bioRxiv - Cell Biology 2020Quote: ... at pH 7.4] and seeded at a density of 50–75 × 103 cells/plate on laminin (Roche) coated plates ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were harvested on ice in Pierce IP lysis buffer and EDTA-free protease inhibitor cocktail (Roche), sonicated ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed 3x with ice cold PBS and lysed with lysis buffer with protease inhibitors (Roche). Cell lysate was centrifuged for 30 min at 15000 g and supernatant was applied to streptavidin-conjugated magnetic beads (Dynabeads® MyOne™ Streptavidin C1 ...
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bioRxiv - Cell Biology 2020Quote: Mammalian cells were lysed in Radioimmunoprecipitation assay buffer (RIPA) buffer with the addition of protease (Roche cOmplete), and phosphatase inhibitors (GB Sciences) ...
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bioRxiv - Immunology 2021Quote: Macrophage cell lysates were lysed in radioimmunoprecipitation assay (RIPA) buffer containing protease inhibitor cocktails and PhosSTOP (Roche). Protein concentration was measured using the BCA Protein Assay Kit (Pierce) ...
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bioRxiv - Immunology 2021Quote: The cell pellets were lysed in 8M urea/50mM Tris pH 8.1/100mM NaCl + protease inhibitors (Roche) and quantified by BCA assay (Pierce) ...
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bioRxiv - Immunology 2021Quote: ... Cells were lysed in IP Lysis Buffer (87787, Pierce™) supplemented with protease inhibitors (Complete mini, Roche), and IP was performed with antibodies against survivin (RnD AF886 ...
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bioRxiv - Neuroscience 2020Quote: ... cells were fixed with 4%PFA and 100 μl of Annexin-V-Alexa 568 labeling solution (Roche) and 50 μM Sytox Green dye (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... and pLenti-Luc-GFP (6 µg) into HEK293T cells using X tremeGENE HP DNA Transfection Reagent (Roche) according to the manufacturer′s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... coli cell lysate (in Buffer C supplemented with a cocktail of protease inhibitors (cOmplete, EDTA-free (Roche)) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were resuspended in AYE media containing a complete mini EDTA-free protease inhibitor cocktail tablet (Roche) and adjusted to an OD600 of 20 ...
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bioRxiv - Microbiology 2021Quote: Cells were washed in PBS and lysed in NTEN buffer supplemented with phosphatase and protease inhibitors (Roche). Protein concentrations were determined using a BCA assay (Pierce) ...
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bioRxiv - Neuroscience 2021Quote: ... for 5 minutes and cells were washed in cold PBS supplied with protease inhibitor cocktail (11873580001, Roche). Cells were stored as dry cell pellet at -80°C until further processed ...
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bioRxiv - Neuroscience 2020Quote: ... The cell suspension was filtered through a 70 µm filter with DNAse (66 U/mL) (Roche Diagnostics). Cell pellet was resuspended in 25% density gradient and centrifuged at 521 g for 20 min at 18° C ...
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bioRxiv - Cell Biology 2021Quote: Cells were re-suspended in lysis buffer containing 2x concentration of PIC (Roche complete, EDTA-free #05056489001) and 2 mM PMSF ...
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bioRxiv - Cell Biology 2021Quote: ... 2 mM β-mercaptoethanol and supplemented with complete EDTA-free cocktail tablets (1 tablet/50ml cells; Roche), 0.01 mg/ml DNase (Sigma ...
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bioRxiv - Systems Biology 2020Quote: ... The cell pellets were then lysed by adding 10 M urea containing complete protease inhibitor cocktail (Roche) and Halt™ Phosphatase Inhibitor (Thermo) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell pellets were lysed in 250 μl IP Lysis Buffer (Pierce) supplemented with complete protease inhibitors (Roche) for 30 minutes at 4 °C ...
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bioRxiv - Microbiology 2021Quote: Culture density was measured with a haemocytometer (BSFs) or a CASY model TT cell counter (Roche Diagnostics) with a 60 µm capillary and exclusion of particles with a pseudo diameter below 2.0 µm (PCFs).
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bioRxiv - Cell Biology 2021Quote: Total proteins were extracted from cells using RIPA lysis buffer with a protease inhibitor cocktail (Roche, Germany). The proteins were quantified using Thermo Scientific Pierce BCA Protein Assay ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were transfected with a total of 1 µg of DNA using Fugene 6 (Roche, Branchburg, NJ) according to the manufacturer’s instructions
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bioRxiv - Molecular Biology 2022Quote: ... the cell culture suspension was supplemented with DNase I and EDTA-free protease inhibitor cocktail tablet (Roche) and lysed by passing through an EmulsiFlex-05 cell disruptor (Avestin ...
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bioRxiv - Molecular Biology 2022Quote: ... the cell culture suspension was supplemented with DNase I and EDTA-free protease inhibitor cocktail tablet (Roche) and cells were lysed by passing through an EmulsiFlex-05 cell disruptor (Avestin ...
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bioRxiv - Molecular Biology 2022Quote: ... Single-cell library quantification is completed using a Roche LightCycle96 using FastStart Essential Green Master (Roche 06402712001) and KAPA Library Quant (Illumina ...
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bioRxiv - Cancer Biology 2022Quote: ... DF-1 cells were transfected with the relevant RCAS viral plasmids using Extreme-Gene 9Transfection reagent (Roche) accordingly to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were fixed with 2% paraformaldehyde/ 2% sucrose for 20 min and stained with DAPI (#10236276001, Roche) to visualize anaphases ...
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bioRxiv - Microbiology 2020Quote: Protein lysates of HEK-293T cells were extracted with RIPA buffer (PIERCE) and protease inhibitor cocktail (Roche). Loading buffer (Life Technologies ...