Labshake search
Citations for Roche :
6001 - 6050 of 7005 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... size and concentration of cDNA libraries were checked using the High Sensitivity NGS kit Fragment Analyzer and qPCR (ROCHE Light Cycler 480). Libraries were sequenced using an Illumina Novaseq 6000 sequencer as single-end 100 base reads ...
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bioRxiv - Neuroscience 2024Quote: ... cDNAs and final libraires were run on an Agilent 4200 TapeStation to check for quality and were quantified using KAPA Library Quantification Kit (Roche, Basel, Switzerland).
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bioRxiv - Genomics 2024Quote: ... Hybridization was carried out with SeqCap RNA Choice Probes using the KAPA HyperCapture Reagent and Bead kit (v2, Roche Sequencing Solutions, Inc.) according to SeqCap RNA Enrichment System User’s Guide (v.1.0 ...
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bioRxiv - Genomics 2023Quote: ... Illumina-compatible sequencing libraries were prepared from 20 ng of purified DNA in 50 μL of TE buffer using the KAPA Hyperprep Kit with Library Amplification Primer Mix (Roche, CAT #07962363001) and 6 cycles of PCR amplification ...
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bioRxiv - Physiology 2023Quote: ... 500ng of total purified RNA was used for library preparation with the KAPA RNA HyperPrep Kit with RiboErase (HMR) for Illumina Platforms (KAPA Biosystems KK8561) according to the manufacturers protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were prepared by the Van Andel Institute Genomics Core from 500 ng of total RNA using the KAPA stranded mRNA kit (v4.16) (Kapa Biosystems, Wilmington, MA). RNA was sheared to 300-400 bp ...
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bioRxiv - Physiology 2024Quote: ... RNA probes were synthesised and labelled using the T7-ScribeTM Standard RNA IVT Kit (Madison, Wisconsin, United States) and Digoxigenin-11-UTP (Roche, Basel, Switzerland). Embryos at various stages were fixed in 4% paraformaldehyde overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... For this 2 µl of RNA sample were mixed with the PCR ingredients of the one-step LightCycler 480 RNA Master Hydrolysis Probes kit (Roche Holding AG), the JFH1-specific probe (5’-6FAM-AAA GGA CCC AGT CTT CCC GGC AA-TMR-3’ ...
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bioRxiv - Microbiology 2023Quote: One microgram of RNA of each isolate was converted into cDNA using the Transcriptor high-fidelity cDNA synthesis kit (Roche, Basel, Switzerland). RT-PCR was performed in 96-well plates using the PowerUp SYBR green master mix (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... The concentration of ligated fragments in each library was quantified with the KAPA Library Quantification Kits for Illumina platforms (Roche/KAPA Biosystems, KK4824) on a Roche LightCycler 480 Instrument (Roche) ...
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bioRxiv - Cancer Biology 2023Quote: ... The bulk transcriptome of these cases was obtained after macrodissection of the whole tumor area on the same block as the one used for the IHC and obtained from FFPE sections using a high-purity FFPE RNA isolation kit (Roche, Basel, Switzerland) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... a maximum of 1 μg of sheared DNA was used for library preparation using the KAPA HTP Prep Kit (KAPA Biosystems, KK8234) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: Cytotoxicity was assessed by measuring the release of lactate dehydrogenase (LDH) into the culture supernatant using the cytotoxicity detection kit PLUS (Roche, Basel, Switzerland). 100 μl cell-free supernatant ...
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bioRxiv - Microbiology 2023Quote: Transcriptional start sites (TSS) of rokL6 and sco1448 were determined by 5’ RACE using a 5’/3’ RACE Kit 2nd generation (Roche, California, US). Total RNA (2 μg ...
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bioRxiv - Biochemistry 2023Quote: ... Final quantification of the RNA-seq library was done with the Library Quantification Kit for Illumina® platforms (KAPA Biosystems, M.A, U.S.A.). The C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The primary antibody was either visualized with the multimer-technology based UltraView Universal DAB Detection kit (Ventana® BenchMark® XT; Roche) or a fluorochrome-labeled secondary antibody (Alexaconjugated ...
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bioRxiv - Pathology 2023Quote: Cell death was assessed in tissue sections using terminal deoxynucleotidyl transferase-mediated dUTP nick- end labeling (TUNEL, Roche TUNEL kit (12156792910; Roche, Basel, Switzerland), as previously described (Cade et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Deletion of FGSG_09786 was further verified by Southern blot with the Digoxigenin High Prime DNA Labeling and Detection Starter Kit I (Roche, Mannheim, Germany). Using a similar strategy ...
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bioRxiv - Cell Biology 2023Quote: ... qRT-PCR analysis was carried out using the qScript One-Step PCR kit (Quanta Biosciences, Gaithersburg, MD) on a LightCycler 96 System (Roche, Indianapolis, IN). Fold-change values were calculated using the 2-ΔΔCt method ...
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bioRxiv - Developmental Biology 2023Quote: ... for 5 min or TRAP staining with a TRAP/ALP Stain Kit (FUJIFILM Wako Pure Chemical Corporation) for 30 min or ALP staining with NBT/BICP solution (Roche; 1:100) for 15 min followed by AR staining prepared from 1% AR Solution at pH 6.3-6.4 (MUTO PURE CHEMICALS CO. ...
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bioRxiv - Evolutionary Biology 2023Quote: The Illumina library was prepared with 250 ng of unsheared DNA using the Kapa Hyperplus Library Preparation Kit with only one third of the volume reactions as described in the manufacturer’s protocol (Kapa Biosystems, Wilmington, MA). The total fragmentation volume was 16.66 μl and was incubated at 37°C for 7:45 min ...
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bioRxiv - Evolutionary Biology 2024Quote: Paired-end sequencing libraries for QTL-Seq analysis were prepared using >1 μg of pooled DNA with a KAPA HyperPrep PCR-free kit (Roche, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... while supernatants from enriched soil/water samples and rectal swabs were extracted using the High Pure PCR Template Preparation kit (Roche, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Samples were transferred to a high pure extender assembly from the High Pure Viral Nucleic Acid Large Volume kit (Roche, Mannheim, Germany) and centrifuged for 8 min with 1,500 rpm at room temperature ...
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bioRxiv - Genetics 2022Quote: ... to evaluate library size and adapter dimer presence before the library concentration was determined via quantitative real time PCR using the KAPA library quantification kits (KK4824, Roche, Basel, Switzerland) on the QuantStudio7 or ViiA7 (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... The libraries were quantified by fluorometry on a Qubit instrument (LifeTechnologies, Carlsbad, CA) and by qPCR with a Kapa Library Quant kit (Kapa Biosystems-Roche) prior to sequencing ...
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bioRxiv - Bioengineering 2022Quote: ... Acetate concentration in the supernatant was measured using the Cedex Acetate V2 Bio HT kit on a Cedex HT system (Roche Custom Biotech). OD600 was measured using a Ultrospec 2100 Spectrophotometer (Amersham Biosciences).
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bioRxiv - Developmental Biology 2022Quote: ... Antisense and sense RNA probes of TaDOF5.6 and TaDOF3.4 genes were synthesized using a DIG RNA Labeling Kit (SP6/T7) (Roche, Cat No. 11175025910, USA). The oligonucleotide primers for in situ hybridization are listed in Table S28.
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bioRxiv - Genomics 2022Quote: ... The resulting libraries were purified following the Nextera XT protocol (0.6× ratio) and quantified by qPCR using Kapa Library Quantification kit (Kapa Biosystems, Cat. KK4824). Four plates were pooled together on a NextSeq 550 high-output flow cell or NextSeq 2000 P2-100 flow cell with 26 bp in read 1 and 50 bp in read 2 ...
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bioRxiv - Genomics 2022Quote: ... The amplification of 16S rRNA genes was conducted using the KAPA2G™ Robust HotStart ReadyMix PCR Kit (Kapa Biosystems, Wilmington, MA, USA) in a total volume of 25□μl ...
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bioRxiv - Plant Biology 2022Quote: ... The enriched biotinylated DNA fragments were subjected to library construction on beads using the KAPA HyperPrep Kit for Illumina (Roche, Basel, Switzerland) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: EMSA DNA fragments were labelled with digoxigenin (DIG) following the manufactureŕs instructions (DIG Gel Shift Kit 2nd Generation; Roche Diagnostics, IN, USA) and 30 fmol of labelled DNA was incubated with different amounts of purified HaWRI1_DBD recombinant protein (0-1000 ng ...
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bioRxiv - Plant Biology 2022Quote: ... The libraries were assessed for size and purity using the Agilent 2100 Bioanalyzer and quantitated by qPCR using a KAPA Biosystems Library Quantitation kit (Roche, Pleasanton, CA). Libraries were normalized ...
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bioRxiv - Pathology 2022Quote: RNA was reverse-transcribed and amplified using the TaqMan Fast Virus 1-Step Master Mix RT-qPCR kit (LifeTechnologies, Carlsbad, CA) on the LightCycler 480 or LC96 instrument (Roche, Indianapolis, IN), and quantified by interpolation onto a standard curve made up of serial tenfold dilutions of in vitro transcribed RNA ...
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bioRxiv - Immunology 2022Quote: ... cells were collected 24 h after incubation with 40 ng/ml IL-10 following the protocol of the High Pure RNA Isolation Kit (Roche, Basel, Switzerland).
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bioRxiv - Microbiology 2022Quote: ... then guide sequences were amplified from gDNA and the plasmid pool by nested PCR using KAPA HIFI Hotstart PCR kit (Kapa Biosystems, KK2501), as previously described in (Young et al. ...
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bioRxiv - Genetics 2023Quote: ... and ceramic beads (Precellys Lysing Kit CkMix, Precellys) in lysis buffer (Lysis Buffer 1X, Milliplex) to which a cocktail of protease inhibitors (Roche Complete Mini) and phosphatase inhibitors (PhosSTOP EASYpack ...
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bioRxiv - Cancer Biology 2023Quote: ... 300 µL of the aqueous phase was mixed with 375 µL ethanol and loaded onto a column from the hiPure miRNA purification kit (Roche, Cat. 5080576001). The samples were then washed and eluted according to the manufacturer’s instructions.
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bioRxiv - Genomics 2023Quote: Approximately 500ng Total RNA was used for RNA-seq library preparation by following the KAPA Stranded RNA-Seq Kit with RiboErase (HMR) sample preparation guide (Cat. No: KR1151, KAPA Biosystems, USA). The first step in the workflow involved the depletion of rRNA by hybridization of complementary DNA oligonucleotides ...
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bioRxiv - Cancer Biology 2022Quote: ... Real-time quantitative PCR was performed using gene-specific qPCR primers listed in Supplementary Materials and the KAPA SYBR Fast qPCR Master Mix (2X) kit (Kapa Biosystems, #KK4602), and ran on CFX96 Real-Time PCR Detection System (BIO-RAD ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Whole-genome libraries were prepared from sheared samples using the KAPA Hyper Plus library preparation kit (KK8514, Roche Diagnostics, Santa Clara, California), NEBNext Multiplex Oligos for Illumina (E7600 ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was synthesized by a reverse transcriptase reaction with 500 ng of total RNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche, Basal, Switzerland), and was used as a template for qPCR with LightCycler 480 SYBR Green I Master (Roche ...
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bioRxiv - Microbiology 2023Quote: ... a 710 bp region was amplified by PCR using previously described primers (45) and the KAPA HiFi HotStart ReadyMix PCR Kit (Roche, Basel, Switzerland) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Average library size and quality control were performed using a Fragment Analyzer (High Sensitivity NGS kit) and qPCR (Roche Light Cycler 480). CUT&RUN libraries were sequenced on a NovaSeq 6000 (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA samples were submitted to the Dana-Farber Molecular Biology Core Facility for mRNA sequencing using the Roche Kapa mRNA Hyper Prep kit (Roche, Basel, Switzerland) with samples sequenced on an Illumina NextSeq or NovaSeq ...
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bioRxiv - Microbiology 2024Quote: ... the RNA-seq directional libraries were generated for 1 μg rRNA-depleted RNA using the KAPA Stranded mRNA-Seq Kit (Kapa Biosystems, KK8421). The rRNA-depleted RNA was fragmented ...
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bioRxiv - Genomics 2024Quote: ... Both DNA samples were also converted into Illumina sequencing libraries with the KAPA HyperPrep library construction kit (Roche Sequencing Solutions, Indianapolis, IN) and paired-end sequenced for 150 cycles on a NovaSeq6000 (Illumina ...
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bioRxiv - Neuroscience 2024Quote: ... The concentration of each library was accurately determined through qPCR utilizing the KAPA library Quantification Kit according to the manufacturer’s protocol (KAPA Biosystems/Roche cat# KK4824) to produce cluster counts appropriate for the Illumina NovaSeq6000 instrument ...
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bioRxiv - Neuroscience 2024Quote: ... Accurate quantification of the final libraries for sequencing applications was determined using the qPCR-based KAPA Biosystems Library Quantification kit (Kapa Biosystems, Inc.). Each library was diluted to a final concentration of 1.4 nM and pooled equimolar prior to clustering ...
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bioRxiv - Neuroscience 2024Quote: ... A DNA library targeting polyadenylated transcripts was constructed for each sample in a 12-cycle PCR (100ng total RNA, KAPA hyper mRNA stranded library prep kit, Roche, catalogue# KK8581). Final cDNA libraries were checked for quality by TapeStation and qPCR (Kapa’s library quantification kit for Illumina Sequencing platforms ...