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Citations for Roche :
5401 - 5450 of 5959 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... 1.5 ml 5 M NaCl, 12.5 μL 2 M Spermidine, final volume brought to 50 ml with dH2O, complemented with one Roche Complete Protease Inhibitor EDTA-Free tablet) ...
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bioRxiv - Neuroscience 2024Quote: ... Ganglia were then treated for 20 min at 37°C with 3 mg/ml collagenase (type I; Roche Diagnostics) and 3 mg/ml dispase II (Roche Diagnostics ...
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bioRxiv - Molecular Biology 2024Quote: ... at 10 mL/g of cell pellet) including protease inhibitors (complete protease inhibitor cocktail tablet, Roche Life Sciences, UK). The suspension was lysed using an Emulsiflex extruding homogenizer (Avestin ...
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bioRxiv - Biochemistry 2024Quote: ... 3 mM magnesium chloride) supplemented with 1 cOmplete protease inhibitor cocktail tablet per 50 ml of lysis buffer (Roche) and mechanically disrupted in a Dounce homogenizer ...
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bioRxiv - Cell Biology 2024Quote: ... and the cell pellet was gently resuspended in 1 ml ice-cold vPBS containing EDTA-free protease inhibitor (Roche). The cells were again pelleted by centrifugation (1000 x g ...
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bioRxiv - Bioengineering 2024Quote: ... The media was removed and 150 μL of serum free RPMI containing 0.25 mg/mL Liberase TL (Roche 054010120001) were added to the samples ...
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bioRxiv - Molecular Biology 2023Quote: ... MIO-M1 cells were transfected with 20 µg/ml of pEGFP or pEGFP-SIK2 vector using FugeneHD (Roche, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK cells were lysed in 1xPBS pH 7.4 containing Protease Inhibitor Cocktail tablet (1 tablet/10 mL) (Roche Diagnostics) and separated in 4–16% gradient Native pre-cast gel (SERVA Electrophoresis GmbH) ...
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bioRxiv - Molecular Biology 2024Quote: ZNF274 expression was induced with 1µg/ml doxycycline and verified via western blot with an anti-HA antibody (1:1000, ref.12013819001, Roche) and anti-beta actin antibody (1:1000 ...
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bioRxiv - Genomics 2024Quote: ... whole blood (14.0 – 17.0 mL per subject) was collected using Cell-Free DNA Collection tubes (Roche Diagnostics; Rotkreuz, Switzerland), with no in-clinic processing (e.g. ...
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bioRxiv - Cell Biology 2024Quote: ... 50 µg of cryoground tissue powder was placed in a 1.5mL lobind tube and brought to 1 mL with lysis buffer (1% triton, 1x PBS, 1x Roche Complete protease inhibitor cocktail ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed by sonication in 50 mL lysis buffer (20 mM Tris pH 8.5, 200 mM NaCl, 10% glycerol, 1 mM PMSF, 2x Roche protease inhibitor tablets ...
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bioRxiv - Genetics 2023Quote: ... 10% Glycerol, 0.2% NP-40, 5mM MgCl2, 5mM DTT, containing one tablet per 10 ml of protease inhibitor cocktail, Roche). Cell debris was removed by centrifugation at 12 Krpm at 4°C for 20 min and the supernatant was recovered ...
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bioRxiv - Neuroscience 2024Quote: ... Cerebral cortex was dissociated by trituration in DMEM/F12 containing 10% FBS and 20 µg/ml DNase I (Roche) using a fire-polished Pasteur pipette ...
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bioRxiv - Cancer Biology 2024Quote: ... Remaining red blood cells were resuspended in extraction buffer (50 mM Tris [pH8.0], 1 mM EDTA [pH8.0], 0.5% SDS and 1 mg / ml Proteinase K [Roche, Nutley, NJ]) and incubated overnight at 55°C ...
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bioRxiv - Developmental Biology 2024Quote: ... ovaries were crosslinked in 250 µL YSS buffer (50 mM pH 8.0 Tris HCl, 50 mM NaCl, 0.1% NP40, 1 U/mL of RNAsin (Roche, 3335402001), protease inhibitor (Thermo Scientific ...
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bioRxiv - Immunology 2024Quote: ... at a ratio of 1:3 (cell:bead) in the presence of 2000 IU/mL recombinant human IL-2 (TECIMTM, Hoffman-La Roche provided by NCI repository ...
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bioRxiv - Immunology 2024Quote: ... at a ratio of 1:3 (cell:bead) in the presence of 2000 IU/mL recombinant human IL-2 (TECIMTM, Hoffman-La Roche provided by NCI repository ...
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bioRxiv - Immunology 2024Quote: ... The digestion was stopped by adding one volume of 2X STOP Buffer (340mM NaCl, 20mM EDTA, 4mM EGTA, 0.02% Digitonin, 50ug/mL RNase A (Cat #3335399001, Roche), 50ug/mL Linear Acrylamide (Cat #K548 ...
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bioRxiv - Immunology 2024Quote: ... followed by tissue digestion for 1 h at 37°C with Iscov’s digestion media containing 1 mg/ml Liberase TL (Roche), and 0.5 mg/ml DNAse (Thermo ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Hippocampus was then sliced with a scalpel and digested for 1 h at room temperature in HBSS supplemented with 0.5 mg/ml collagenase D (Roche) and 10 µg/ml DNase I (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2024Quote: ... kidney was thawed in 1 ml of hypotonic buffer (10 mM Tris pH 7.5, 150 mM sucrose including EDTA-free cOmplete protease inhibitor (Roche)) and homogenized using bead mill (5 cycles ...
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bioRxiv - Biochemistry 2023Quote: ... benzonase (to 1 µg/mL) and cOmplete EDTA-free Protease Inhibitor Cocktail (Roche, 2 tablets/L initial culture volume) were added to the cell suspension and stirred at RT for 30 mins ...
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bioRxiv - Biophysics 2024Quote: ... were resuspended in 20 mM Tris (pH 7.4) and 300 mM NaCl (50 mL volume) containing complete protease inhibitor cocktail (Roche) and lysed by soni- cation ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was stored as flow through and the beads were washed 6 times with 1 ml RIPA buffer complemented with 0.25× protease inhibitor (cOmplete, Roche). Bound proteins were eluted from agarose beads with 1 bead volume of Laemmli buffer heated at 95°C for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... bacterial cell pellets were resuspended in 1.5 ml of lysis buffer (25 mM Tris pH 7.5, 150 mM NaCl, benzonase, EDTA-free protease inhibitor cocktails (ROCHE)) and disrupted at 4°C with 0.1 mm glass beads and using a PRECELLYS 24 homogenizer ...
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bioRxiv - Microbiology 2022Quote: ... and the right inferior lung lobes were digested in 50 μL of 5 mg/mL of Liberase Tm (Roche) and 12.5 μL of 10 mg/mL of DNase I (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... cells were released by incubating gels in a cell culture incubator in a protease solution of 2.5 mg/mL collagenase D (Roche) in PBS until gels were fully degraded ...
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bioRxiv - Microbiology 2022Quote: ... according to the manufacturer’s instructions and maintained in RPMI GlutaMax supplemented with 10% FCS and 30 U/ml recombinant IL-2 (Roche).
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bioRxiv - Biochemistry 2022Quote: Cells were thawed and resuspended in 250 mL of ice-cold hypotonic lysis buffer (10 mM HEPES, pH 7.5, containing Roche protease inhibitor tablets and 0.1 mU/mL apyrase to prevent separation of the GPCR-miniG complex) ...
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bioRxiv - Molecular Biology 2022Quote: ... flow cytometry analysis was performed on cell suspensions from kidney homogenates that were digested with 0.2 mg/ml liberase (Roche) and 0.1 mg/ml DNase I (Roche ...
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bioRxiv - Biochemistry 2023Quote: ... 0.75%/0.15% dodecyl maltoside/cholesteryl hemisuccinate (DDM/CHS) with 2 mg/ml iodoacetamide and a Protease Inhibitor tablet (Roche). After 4 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... was purified via Immobilized Metal Affinity Chromatography (IMAC) using a prepacked 5 mL cOmpleteTM His-Tag purification column (Roche) equilibrated in HEPES-buffered saline buffer (HBS ...
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bioRxiv - Developmental Biology 2023Quote: ... dissected tissues were dissociated for 30 mins at 37 °C in 200ul of LiberaseTM (Roche, 100ug/ml in PBS). 2 mL of cold PBS (without ca/Mg ...
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bioRxiv - Neuroscience 2023Quote: ... Tissues were washed in HBSS and then dissociated with 1mg/ml of collagenase and dispase (Roche, Indianapolis, IN, USA) for 45 mins at 37 degrees ...
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bioRxiv - Genomics 2023Quote: The captured DNA was amplified for ten cycles using KAPA HiFi HotStart ReadyMix (6.25 ml, Roche, catalog number 7958935001) and purified in 1.8 x volume of Agencourt AMPure beads (Beckman Coulter ...
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bioRxiv - Developmental Biology 2023Quote: ... and thus we dechorionated Stage 24-25 embryos (or 2 to 2.5 dpf) pupfish by incubating them on 20 mg/ml Pronase (Roche) in water for 2.5 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... Mouse atria were minced and digested with mild agitation for 30 minutes at 37°C in DMEM containing 1% P/S and 0.14 Wunsch U ml-1 Liberase (5401119001, Roche). MAFs were enriched via negative selection with magnetic beads against mouse CD45 (leukocytes) ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5 mM dithiothreitol (DTT)) containing 10 µg/ml phenylmethyl-sulfonyl fluoride (PMSF) and Complete EDTA-free protease cocktail (Roche). Cells were incubated on ice for 10 min and lysed by passing five times through a 27-Gauge needle ...
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bioRxiv - Biochemistry 2023Quote: ... prior to resuspension in 25 mL ice-cold water with another tablet of cOmplete™ ULTRA protease inhibitor (Roche), followed by centrifugation for 30 minutes at 15000×g and 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Frozen pellets were thawed on ice and lysed with 20mls buffer per gram of pellet (50mM HEPES, 500mM NaCl, 0.5mM TCEP, 1U/ml DNase supplemented with Roche cOmplete™ ...
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bioRxiv - Genetics 2023Quote: ... incubated the islets for 10 min at 37°C in 2 mL PBS with 4U Dispase I (Roche Diagnostics) / 2U DNase I (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... the livers were cut and separated with forceps and digested in 3mg/mL Liberase (589994, Roche ® Life Science) and 3mg/mL DNase I (Roche ...
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bioRxiv - Immunology 2023Quote: ... The fixed cells were collected by scraping in 0.5 ml of ice cold 1x PBS supplemented with 1x cOmplete proteinase inhibitor (Roche) for U2OS and by centrifugation for SUDHL4 at 1000g for 5 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... The epidermis and dermis layers were separated after incubation at 37°C for 1h in 0.4 mg/mL dispase II (Roche). The epidermis was collected and then cut into small pieces using scalpels and incubated for 30 minutes with agitation at 37°C in RPMI containing 10% FCS and 0.5 mg/mL DNAse I ...
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bioRxiv - Molecular Biology 2023Quote: ... 75 μl of the sample were treated for 30 min at 50°C with 1.2 mg/ml Proteinase K (Roche) in Proteinase K Buffer (100 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2023Quote: ... Minced lung was enzymatically dissociated to a single cell suspension using 1 mg/mL collagenase A (Roche, Cat# 10103578001), 2-4U/mL elastase (Worthington ...
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bioRxiv - Physiology 2023Quote: Approximately 50 mg of adipose tissue was homogenized in 1 mL of lysis buffer (250 mM sucrose, 50 mM Tris Cl, pH 7.0, with protease inhibitor cocktail (11873580001, Roche)) ...
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bioRxiv - Immunology 2023Quote: ... Brain and spinal cord were cut into pieces and digested for 30 min at 37 °C with collagenase D (3.75 mg/ml; Roche) and DNase I (1 mg/ml ...
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bioRxiv - Systems Biology 2023Quote: ... The left lobes of the lung were then inflated with digestion solution containing 1.5 mg/mL of Collagenase A (Roche) and 0.4 mg/mL DNaseI (Roche ...