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Citations for Roche :
5301 - 5350 of 5959 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... Trypsin activity was then inhibited by adding to the homogenate bovine serum albumin (BSA) fatty acid free (0.25 mg/mL) and protease inhibitor cocktail (PIC, Roche).
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bioRxiv - Microbiology 2021Quote: ... 109 CFUs of bacteria were resuspended in 150 µL of buffer TE (10 mM Tris-HCl, 1 mM EDTA, pH 8.0) with 5 mg mL-1 of lysozyme (Roche) for 5 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Nuclei pellets were resuspended in 1 ml nuclei permeabilization buffer (5 % BSA, 0.2 % IGEPAL-CA630, 1mM DTT and cOmpleteTM, EDTA-free protease inhibitor cocktail (Roche) in PBS ...
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bioRxiv - Microbiology 2019Quote: ... The cell pellets were resuspended in 1 ml ZAP buffer containing a protease inhibitor (50 mM Tris-HCl pH 7.5, 200 mM NaCl, 1 complete tablet per 50 ml buffer (Roche)) and cells disrupted three times for 45 sec at 6 m/s using an MP Biomedicals™ Fastprep-24 machine ...
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bioRxiv - Immunology 2021Quote: ... ankle joints were teased apart and shaken at 110 rpm at 37°C for 25 minutes with 2.68mg/mL collagenase D (Roche) in RPMI 1640 (Gibco ...
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bioRxiv - Biophysics 2020Quote: ... a 500 mL cell pellet of Sf9 cells was thawed in 50 mL lysis buffer (25 mM HEPES pH 7.4, 1 M KCl, 10% glycerol, Roche protease inhibitor ...
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bioRxiv - Neuroscience 2020Quote: ... the small intestines were cut into 2-5mm2 pieces and put into digestion solution (0.75mg/ml Liberase TH Research Grade (Roche), 12 U/ml Dispase ...
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bioRxiv - Neuroscience 2022Quote: ... Pronase solution was removed and 2 ml of reconstitution solution (1% fetal bovine serum + DNAse (1:10,000, Roche #47167280001)) in oxygenated ACSF was added ...
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bioRxiv - Biochemistry 2022Quote: ... 10 mM MgCl2, 10% glycerol, 300 mM NaCl, 0.2 U/mL DNase 1, 1 mM PMSF, complete proteinase inhibitor mixture; Roche) and homogenized with an EmulsiFlex for 20 min ...
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bioRxiv - Cell Biology 2022Quote: ... The pellet was resuspended with 3 ml S1 solution (0.25 M sucrose, 10 mM MgCl2, 1x Complete protease inhibitor cocktail (Roche)) by pipetting up and down ...
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bioRxiv - Cell Biology 2022Quote: ... the dorsal skin of C57BL/6 neonates was collected and incubated overnight with 1 mg/mL collagenase/dispase (Roche). The dermis and epidermis were separated by incubating the skin with 0.25% trypsin/10 mM EDTA in phosphate-buffered saline (PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... 5mM DTT) supplemented with 0.325 mg/mL lysozyme and a cocktail of protease inhibitors (Roche, Complete™ EDTA free). Samples were sonicated ...
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bioRxiv - Developmental Biology 2022Quote: ... The alkaline phosphatase activity was visualized by incubating the specimens in NTMT containing 0.07 mg/ml nitroblue-tetrazolium chloride (Roche), and 0.035 mg/ml 5-bromo-4-chloro-3-indolyphosphatase (Roche) ...
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bioRxiv - Cell Biology 2022Quote: Tissue leukocytes were extracted from surgically dissociated lung tissue by enzymatic digestion with 2ml enzyme mix (RPMI with 0.625 mg/ml collagenase D (Roche), 0.85 mg/ml collagenase V (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... filtered conditioned medium was loaded onto a 5 ml cOmplete™ His-tag Purification Column (Roche Diagnostics, Vilvoorde, Belgium). The protein was eluted with 250 mM imidazole in HBS after which the imidazole was removed using a HiPrep™ 26/10 Desalting column (GE Healthcare ...
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bioRxiv - Pathology 2022Quote: Kidneys and livers were minced into small pieces and digested (1mL; RPMI, GibcoTM ThermoFisher Scientific # 61870044, 0.2 mg/mL Liberase, Roche # 5401127001 ...
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bioRxiv - Immunology 2022Quote: Enrichment and staining of lung immune cells were performed as detailed elsewhere [2,3] after treatment with 400 U/ml type IV collagenase and DNase I (Roche) for a 30-minute incubation at 37°C and homogenization by use of GentleMacs (Miltenyi Biotech) ...
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bioRxiv - Microbiology 2022Quote: ... lung samples of infected mice were harvested and homogenized in 1 ml PBS using the MagNA Lyser (Roche Diagnostics). The homogenates were clarified by centrifugation at 15,000 rpm for 5 min ...
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bioRxiv - Biophysics 2022Quote: ... The sinoatrial node explants from old animals were first incubated for 20 min in Low-Calcium Tyrode’s containing twice the concentration of collagenase B used in the young (Cf = 1.08 U/ml, Roche 11088807001), followed by the same 30 min digestion with the three other enzymes at the same concentration used for young tissues ...
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bioRxiv - Molecular Biology 2022Quote: ... β-DDM in 60 ml of 20 mM Bis-tris propane pH 8.0 with 10 % glycerol and EDTA-free Complete protease inhibitors (Roche) for 20 min at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The clarified cell lysate was bound to 10 mL of cOmplete His-Tag purification resin (Roche, Germany, 30 min), washed with 1 L base buffer ...
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bioRxiv - Microbiology 2022Quote: Fresh pre-cut pancreatic tissue (2 × 2 mm) were digested with a solution of collagenase P in HBSS-1% HEPES (0.75 mg/mL, Roche) at 37°C for 7 min with shaking ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were washed 2x in PBS + 0,1% Tween-20 (PBS-T) followed by 15 min permeabilization in 1- μg/ml proteinase K (Roche) and 3 washes in PBS-T ...
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bioRxiv - Immunology 2020Quote: Lungs were aseptically isolated from mice and incubated at 37°C in 10% FBS RPMI media containing 1.4 mg/ml collagenase A (Roche) and 30 μg/ml DNase I for 60 min ...
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bioRxiv - Microbiology 2021Quote: ... which was freed from muscle tissue with a scalpel and floated on PBS containing 2.86 mg/ml dispase II (Roche) for 60min with the epithelial side facing upwards to separate the epithelial tissue from the lamina propria ...
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bioRxiv - Genomics 2021Quote: ... and then resuspended in 3 mL of lysis buffer (160 nM NaCl, 20 mM, Tris-HCl pH7.5, 1 mM EDTA, protease inhibitor cocktail (Roche)) ...
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bioRxiv - Neuroscience 2020Quote: Whole cerebral organoids were washed three times in 1× PBS and dissociated in 2 mL of Accutase (StemPro) containing 0.2 U/μL Dnase I (Roche) for 45 min at 37°C ...
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bioRxiv - Immunology 2020Quote: ... Small intestines were minced into small pieces and digested in RPMI medium containing 0.1 mg/ml DNase I (Roche) and 100 mg/ml Collagenase VIII (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... For experiments in which islets were analyzed using flow cytometry single cell suspensions were generated by digestion with 0.4 Wunsch Units/ml Collagenase D (Roche) and 250 µg/ml DNAse I (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... Platelets (3 x 105 platelets/μl) were allowed to spread on the coated surface after the addition of 0.01 U/ml thrombin (Roche) and Ca2+ ...
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bioRxiv - Microbiology 2020Quote: ... Mycelia pellets were thawed on ice and resuspended in 0.4 mL ice-cold lysis buffer (20 mM Tris pH 8.0, 5 mM EDTA, 1x EDTA-free protease inhibitors [Roche]) and sonicated (5x 15 sec on/15 sec off at 5-micron amplitude) ...
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bioRxiv - Immunology 2020Quote: ... the pellet was resuspended in 50 mL membrane wash buffer supplemented with 1X protease inhibitor (Roche cat no. 04693159001) and 10mg/mL lysozyme and let shake on ice for 30 minutes before sonication at 70% amplitude ...
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bioRxiv - Microbiology 2021Quote: ... 150 mM NaCl) and resuspended in 1 ml Tris-Buffered saline supplemented with protease inhibitor (cOmplete Protease Inhibitor, Roche). Bacterial lysis was done by enzymatic cell wall degradation (30 min at 37°C with 10K/ml mutanolysine ...
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bioRxiv - Biochemistry 2021Quote: ... Pellets were then incubated in 300 ul 10mM Tris-HCl (pH 7.5) solution containing 100ug/mL proteinase K (Roche) at 60C overnight followed by 30 min at 90C to denature Proteinase K ...
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bioRxiv - Biochemistry 2021Quote: ... Active Superose 6 fractions with an apparent molecular mass of 400 - 600 kDa were pooled and supplemented with 0.25 mg/ml insulin (Roche). Pooled fractions were applied to a Poros 20 HE column (Applied Biosystems ...
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bioRxiv - Biophysics 2021Quote: ... Platelet activation was achieved by complementing the Tyrodes buffer with 2 mM Ca2+ and the addition of 0.01 U/ml Thrombin (10602400001, Roche) to the platelet suspension.
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bioRxiv - Immunology 2022Quote: ... The remaining tissue was then incubated with constant shaking at 37C in RPMI media containing 0.1mg/ml collagenase/dispase (Roche) and 20µg/ml DNase (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... The pellet was resuspended in lysis buffer (50mM Tris-HCl, pH 7.4; 150mM NaCl; 10ug/mL DNase I; one tablet Roche cOmplete EDTA free protease inhibitor cocktail ...
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bioRxiv - Microbiology 2022Quote: ... cells were lysed with lysis buffer (final concentration: 50 μg ml−1 lysozyme, 0.8% NP-40, 1 × protease inhibitor (Roche), 250 U ml−1 benzonase ...
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bioRxiv - Biophysics 2022Quote: ... resuspended in 20mM Tris-HCl pH 8.0 (30 ml per liter of culture) containing complete EDTA-free protease inhibitor cocktail (Roche) and lysed using a Microfluidizer M-110L (Microfluidics ...
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bioRxiv - Immunology 2022Quote: ... Collected tissue was mechanically dissociated by chopping followed by enzymatic digestion for 45 minutes at 37°C in DMEM containing collagenase D (1.5 mg/ml, Roche) and DNase I (60 U/ml ...
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bioRxiv - Immunology 2019Quote: Purified B cells were cultured either in medium alone or in the presence of 1.56-25ng ml− of recombinant BAFF for the indicated time and stained with Annexin V (Roche) and 7-aminoactinomycin D (7-AAD ...
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bioRxiv - Developmental Biology 2020Quote: ... The resulting organoids were further digested with warm 0.25% trypsin for 1 min with constant pipetting followed by treatment with 5 mg/mL Dispase (Roche) for 5 min in at 37°C ...
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bioRxiv - Immunology 2020Quote: ... minced with scissors and digested at 37°C for 20-30 minutes in digestion buffer (0.52 U/ml Liberase TM [Roche] and 60 U/ml DNase I [Roche] in RPMI 1640 [Cellgro] with 5% fetal bovine serum) ...
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bioRxiv - Pathology 2020Quote: Adult mouse pancreas from Sox9: eGFP transgenic mice (33) were harvested and digested in 1.4 mg/mL collagenase-P (Roche) at 37 °C for 20-30 min ...
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bioRxiv - Immunology 2020Quote: Mice were euthanized and lungs were gently homogenized in HEPES buffer containing Liberase Blendzyme 3 (70 μg/ml; Roche) and DNaseI (30 μg/ml ...
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bioRxiv - Genetics 2021Quote: ... Isolation of cfDNA from 2 mL of plasma was performed using the semi-automated MagNA Pure 24 System (Roche) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... at 37°C in a 0.4 μm filtered solution of 0.5 mg/mL collagenase D (Roche, Indianapolis, IN, USA), 1 Unit/mL Dispase (Stemcell Technologies ...
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bioRxiv - Microbiology 2021Quote: HEK293T cells transiently transfected with WT EphA2-eYFP were selected with 1.6 mg/ml G-418 solution (Roche; 4727878001) for 12 days to generate a stable cell line ...
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bioRxiv - Synthetic Biology 2021Quote: ... 0.1% SDS) and resuspension of the nuclei in 0.9 mL Covaris shearing buffer (with 1× protease inhibitors complete mini (Roche)) ...