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Citations for Roche :
5251 - 5300 of 5959 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 10 μg/mL DNase I) containing a single dissolved cOmplete EDTA-free Protease Inhibitor Cocktail tablet (Roche: 11873580001). The mixture was first gently homogenized with a Biospec Tissue Tearor (Dremel ...
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bioRxiv - Cell Biology 2023Quote: ... or MDCK-Ecad Res cells were pre-treated with Mitomycin C at a concentration of 10μg/ml (Roche) for a duration of 1 hour to inhibit cell proliferation ...
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bioRxiv - Biochemistry 2023Quote: ... 10% glycerol and 0.1% NP-40 supplemented with 0.1 mg of lysozyme/ml and complete inhibitor cocktail (Roche)) and sonicated to disrupt the cells ...
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bioRxiv - Plant Biology 2024Quote: ... supplemented with 1 tablet of protease inhibitor cocktail per 10 ml buffer (cOmplete™ Proteasehemmer-Cocktail, ©Roche) with an ultrasonic homogenizer (Hielscher Ultrasonics UP200St ...
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bioRxiv - Microbiology 2024Quote: ... The remaining 90% of each sample was immunoprecipitated with 1:1000 anti-HA antibody (0.5 mg/mL Roche Rat Anti-HA High Affinity [11867423001] ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed with wash buffer (50 ml total H2O with 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, and one Roche Complete protein inhibitor tablet (Millipore ...
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bioRxiv - Microbiology 2024Quote: ... The pellet was subjected to enzymatic lysis with 90 µl of 50 mg/ml lysozyme (Roche, Mannheim, Germany) and 50 µl of 5 KU/ml mutanolysin from Streptomyces globisporus ATCC 21553 (MilliporeSigma ...
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bioRxiv - Immunology 2024Quote: ... Mouse lungs were excised and lightly homogenized in HEPES buffer containing Liberase Blendzyme 3 (70 μg/ml; Roche) and DNaseI (30 μg/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... Reactions were stopped by adding 0.5 μl ethylenediaminetetraacetic (0.5 M EDTA) and 1 μl Proteinase K (19 mg/ml, Roche), and incubated at 50°C for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... The pellet was washed in buffer A three times and then homogenized in 400 ul of buffer B (250 mM sucrose, 10 mM Tris HCl [pH 8.0], 10 mM MgCl2, 1 mM EGTA, 1X protease-inhibitor cocktail [11697498001, Roche], 0.1% Triton X-100, and 0.25% NP-40) for 10 min at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... Pellets were suspended in 50 ml of denaturation buffer (8 M GdmCl, 50 mM Tris-HCl, pH 8.0) with one protease inhibitor tablet (Roche) per L medium ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was collected and incubated with 20 ml of Eps8L2 Lysis Buffer without lysozyme nor Pefabloc® (Roche), and 4% Triton X-100 for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... The pellet was lysed with 20 ml of Eps8L2 Lysis Buffer (10 mM Tris.HCLpH8, 150 mM NaCl, 1 mMEDTA, lysozyme 0.1 mg/ml, Pefabloc® (Roche) 0.5 mg/ml ...
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bioRxiv - Cell Biology 2019Quote: ... mechanically disintegrated and partially digested in a solution of collagenase and trypsin [2 mg/ml collagenase (Roche or Sigma), 2 mg/ml trypsin (Dutcher Dominique or Sigma) ...
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bioRxiv - Cell Biology 2020Quote: Genomic DNA was extracted from sorted cells by first decrosslinking cells in a solution of 10 mM Tris pH 7.5 and 10 mg/mL Proteinase K (Roche) in PBS at 55°C for 24 hours ...
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bioRxiv - Immunology 2021Quote: ... Culture media was supplemented with 500 U/mL human IL-2 (Tecin from Roche, kindly provided by the NIH) starting on culture day 2 ...
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bioRxiv - Developmental Biology 2021Quote: ... 2% blocking powder from Roche, 5X SSC, 0.1% TritonX, 0.1% CHAPS from Sigma Aldrich, 50% formamide, 1 mg/ml tRNA from Roche, 5 mM EDTA from Sigma and 50 μg/ml Heparin from Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were hybridized with 30μg/mL of Digoxigenin (DIG) conjugated telomere probe (CCCTAACCCTAACCCTAACCCTAA-DIG, IDT) in DIG Easy Hyb buffer (Roche) overnight at 42°C ...
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bioRxiv - Immunology 2021Quote: ... the fibrin gel portions were cut into small pieces to be properly digested in an enzymatic solution of Collagenase/Dispase (0.5 mg/mL) (Roche) and DNase I (0.5 mg/mL ...
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bioRxiv - Biophysics 2021Quote: ... The cell pellet was resuspended in 1 ml of polysome lysis buffer supplemented with EDTA-free protease inhibitors (Roche) and was grounded in liquid nitrogen using an RNase-free mortar and pestle ...
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bioRxiv - Developmental Biology 2021Quote: ... Dissected lungs and kidneys were washed in DMEM/F12 and then incubated in 200ng/ml Collagenase/Dispase (Roche; 10269638001) in DMEM/F12 for 10 min (kidney ...
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bioRxiv - Neuroscience 2022Quote: ... DRGs from E13.5 embryos were dissected and placed in droplets of 2 mg/ml collagen (Roche Diagnostic, catalog#11179179001) together with COS1 aggregates transfected with either secreted Myc-Sema3A or control PAY1-GFP ...
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bioRxiv - Cancer Biology 2022Quote: Pancreatic tumors harvested from PKT mice were enzymatically digested using solution of 0.6 mg/ml of collagenase P (Roche), 0.8 mg/ml Collagenase V (Sigma Aldrich) ...
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bioRxiv - Developmental Biology 2022Quote: ... fins were incubated for 20 min at 28°C with vigorous shaking in a solution of Liberase DH Research Grade (0,05mg/ml in 1x PBS, Roche). Cell suspensions were passed through a 30 μm filter (CellTricks ...
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bioRxiv - Immunology 2022Quote: The lung tissue from infant and adult post-septic mice was digested with Liberase TL (0.2 mg / mL, Roche) and DNase I (0.5 mg / mL ...
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bioRxiv - Genomics 2020Quote: ... Day 4 (HH21-24) and day 7 (HH30-31) ventricular samples were digested in 1.5mg/mL collagenase type II/ dispase (Roche) for one cycle of 20 minutes and one cycle of 10 minutes under mild agitation at 37°C ...
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bioRxiv - Immunology 2019Quote: ... MLN from indicated groups of mice were dissected into small pieces and digested with freshly made enzyme mix comprised of RPMI-1640 containing 0.12 mg/ml Collagenase P (Roche), 0.4 mg/ml Dispase (Roche ...
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Caspase-8 Modulates Angiogenesis By Regulating A Cell Death Independent Pathway In Endothelial CellsbioRxiv - Developmental Biology 2019Quote: ... Tissue from Casp-8WT and Casp-8ECko pups from the same litter were pooled and digested in collagenase I/DNAse I (0.1mg/ml, Roche) at 37°C ...
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bioRxiv - Genetics 2019Quote: ... DNA was eluted in 100μl of TE + 1%SDS and the beads were washed and eluted a second time with 100μl of TE + 1%SDS + 5 μl of 20mg/mL Proteinase K (Roche). Following overnight incubation at 65°C ...
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bioRxiv - Developmental Biology 2019Quote: ... whole E16.5 – E18.5 lungs were manually dissociated with a pestle in separate microcentrifuge tubes and then incubated with 2.5 mg/mL Collagenase A (Roche) and 20 ug/mL DNAse 1 (Sigma ...
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bioRxiv - Cancer Biology 2019Quote: ... coli cells containing the overexpressed RNF114 fusion protein were re-suspended in 80 mL of lysis buffer (50 mM Tris, pH 7.5, 150 mM NaCl, 2 Roche protease inhibitor tablets [without EDTA] ...
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bioRxiv - Developmental Biology 2019Quote: ... 1X Denhardt solution) containing 1μg/ml DIG labeled RNA probes (prepared with a DIG RNA labeling mix, Roche, 11277073910) for overnight at 65°C in a humid chamber ...
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bioRxiv - Microbiology 2019Quote: ... The solution was then supplemented with 20 mM MgSO4 and treated with 50 μg mL-1 RNase A (Roche) and 10 μg mL-1 DNase (Sigma-Aldrich ...
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bioRxiv - Genomics 2019Quote: ... Samples from the head skin of Prss53-/- and WT rabbits were homogenized and lysed in RIPA buffer supplemented with 2.5 μL/mL protease inhibitor cocktail (Roche) on ice for 30 min ...
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bioRxiv - Cell Biology 2019Quote: ... and the skin samples were cut into small pieces and incubated in 0.33 mg/ml of Liberase TM Research Grade (05401119001, Roche) at 37 °C for 30 min ...
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Genomic organization of the autonomous regulatory domain of eyeless locus in Drosophila melanogasterbioRxiv - Genetics 2021Quote: ... The fixed embryos were resuspended in 2.5 ml of ice-cold lysis buffer (10 mM Tris-pH-8, 10 mM NaCl, 0.2% NP40 with Roche protease inhibitor cocktail freshly added ...
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bioRxiv - Cell Biology 2021Quote: ... for 90 min at 37 °C and digested overnight at 37 °C in 1.5 mg/ml collagenase B solution (Roche). Then ...
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bioRxiv - Immunology 2021Quote: ... Lungs were dissected and digested for 30min at 37°C using RPMI media (2% FBS+ Pen/Strep, glutamine, 10mM HEPES) containing 0.5mg/mL Collagenase D (Roche). The digested fragments were passed through a 70μm cell strainer (Greiner bio-one ...
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bioRxiv - Microbiology 2021Quote: ... frozen pellets were thawed on ice in 50 mL conical flasks with pre-chilled lysis buffer (50 mM HEPES – KOH pH 7.6, 500 mM KCl, Roche Protease Inhibitors tablet (0.5 tablet/L of culture) ...
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bioRxiv - Neuroscience 2021Quote: ... and the reaction was stopped with the addition of 100μL fetal bovine serum (FBS; Anprotec, Bruckberg, Germany) and 100μL DNase I (0.5 mg/mL; Roche, Basel, Switzerland). After dissociation ...
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bioRxiv - Cancer Biology 2021Quote: Human tumor samples were collected on different days right after surgery and digested in Hank’s Balanced Salt Solution supplemented with 2 mg/mL collagenase A (Roche), 2.5 U/mL hyaluronidase (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2020Quote: Muscles were dissected and minced to a pulp using forceps then digested with collagenase B solution (0.75 U/mL final, from Roche) with dispase II (1.2 U/mL final ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were harvested and incubated in buffer A (50 mM MMT pH 8.0/300 mM KCl/10 mM MgCl2/5 % glycerol) containing 1 g L-1 lysozyme/2.5 U mL-1/SmDNAse//complete protease inhibitor cocktail (Roche) for 1 h prior to lysis using EmulsiFlex C5 (Avestin ...
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bioRxiv - Immunology 2020Quote: ... The red blood cell layer was lysed with 2 mL of Red Blood Cell Lysis Buffer (Cat# 11814389001, Roche) in a 15 mL tube ...
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bioRxiv - Neuroscience 2020Quote: ... They were rehydrated in decreasing concentrations of methanol and embryos 24 hpf and older were permeabilized using Proteinase K (10μg/ml, Roche). Embryos were refixed in 4% paraformaldehyde prior to hybridization ...
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bioRxiv - Cancer Biology 2020Quote: Tumors were mechanically dissociated and digested for 45 min at 37 °C in RPMI 1640 with 37.5 µg/mL Liberase TM (Roche) and 8,000 U/mL DNase I ...
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bioRxiv - Cell Biology 2021Quote: To isolate mammary epithelial cells the third and fourth pairs of mammary glands were removed from 4 to 6-week-old s-SHIP–GFP transgenic (Tg11.5kb–GFP) mice, minced with scissors and digested in digestion medium (DMEM/F12, 2 mg ml−1collagenase I (Roche), 5 mg ml−1 insulin (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... Nuclei pellets were resuspended in 1 ml nuclei permeabilization buffer (5 % BSA, 0.2 % IGEPAL-CA630, 1mM DTT and cOmpleteTM, EDTA-free protease inhibitor cocktail (Roche) in PBS ...
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bioRxiv - Biochemistry 2020Quote: ... 3,000 rpm) and resuspended in 20 mL lysis buffer (buffer A including one EDTA-free protease inhibitor tablet (Roche) and 10 mg/mL lysozyme ...
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bioRxiv - Microbiology 2021Quote: ... 200 μg each lysate was pre-cleared with 20 μl of Protein A agarose beads (3 mg/ml, Roche) in 200 μl for 1 hour at 4°C on a Labquake Rotator (Barnstead Thermolyne) ...