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Citations for Roche :
5351 - 5400 of 5959 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... we collected feces of 90 adults that had fed on Arabidopsis wild type leaves for one day in a total volume of 1 mL 20 mM MES buffer pH 6.5 containing protease inhibitors (cOmplete, EDTA-free, Roche). After homogenization with metal beads for 3 min at 25 Hz in a TissueLyzer II and centrifugation at 4 °C for 10 min at 16,000 × g ...
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bioRxiv - Genetics 2021Quote: ... Bacteria were pelleted and resuspended in lysis buffer (50 mM Tris pH 8.8, 200 mM NaCl, 2 mM DTT, 1 mM PMSF, 1 mg/ml lysozyme, 1x Roche cOmplete™ EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Microbiology 2019Quote: ... cell pellets were resuspended in 200 µl of TE buffer supplemented with 2.5 mg/mL lysozyme and 1x cOmplete mini EDTA-free protease inhibitor cocktail (Roche), samples were incubated at 37°C for 30 min ...
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bioRxiv - Developmental Biology 2021Quote: Adult and E18.5 mouse pancreas from Sox9-eGFP transgenic mice (Gong et al., 2003) were harvested and digested in 1.4 mg/mL collagenase-P (Roche) at 37 °C for 20-30 min ...
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bioRxiv - Neuroscience 2019Quote: ... one series of frozen brain slices was homogenized in 1 ml PBS containing complete protease inhibitor (Roche Applied Science) and 1 mM AEBSF (Sigma ...
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bioRxiv - Neuroscience 2020Quote: Cultured cells were lysed in RIPA buffer containing protease inhibitor cocktail (1 tablet/15 mL) (Roche Diagnostics, Mannheim, Germany), sonicated and stored at −80°C until analysis ...
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bioRxiv - Cancer Biology 2019Quote: ... 1 mM Na3VO4 and supplemented with a protease inhibitor cocktail (1 tablets to 10 ml of lysis buffer, Roche) for 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... 0,25 % NP-40 and supplemented with a protease inhibitor cocktail (1 tablets to 10 ml of lysis buffer, Roche) for 10 minutes at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... and incubated on a rocker for 10 minutes with 0.4 mL RIPA lysis buffer containing protease inhibitors (cOmpleteTM tablets, Roche) and phosphatase inhibitors (PhosSTOPTM tablets ...
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bioRxiv - Genomics 2021Quote: ... The skin pieces were then placed in an enzyme cocktail consisting of 3.3 mg/ml Collagenase-P (Roche, 11249002001), 3.3 mg/ml Dispase II and 1.5 mg/ml DNase I (STEMCELL Technologies ...
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bioRxiv - Immunology 2021Quote: ... brain and dura mater were chopped and subject to enzymatic digestion in Hanks’ Balanced Salt Solution (HBSS) containing 2.5 mg/mL collagenase D (Roche) and 0.1 mg/mL DNase I (Roche ...
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bioRxiv - Immunology 2021Quote: ... Livers and liver tumours were excised and then minced with scissors before incubation in RPMI/10% (v/v) FBS/2mM EDTA/1.2mg/mL Collagenase D (Roche)/1mg/mL DNase I (Roche ...
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bioRxiv - Genetics 2021Quote: ... 0.1% SDS) and resuspension of the nuclei in 0.9 mL Covaris shearing buffer (with 1 × protease inhibitors complete mini (Roche)) ...
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bioRxiv - Cell Biology 2021Quote: ... for 90 minutes at 37°C and digested overnight at 37°C in 1.5 mg/ml collagenase B solution (Roche) under continuous agitation ...
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bioRxiv - Neuroscience 2021Quote: The tissue was dissociated in 300 µL of 0.25% trypsin-EDTA solution supplemented with 10 U/mL recombinant DNase I (Roche) for 15 minutes at 37°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 30 animals per group were dissected in CMFB (CMF containing 0.5% BSA) and dissociated using 1:50 Liberase (2.5mg/mL, Roche 5401135001) in CMFB at 30°C with agitation at 300 rpm for 30 minutes on an Eppendorf ThermoMixer ...
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bioRxiv - Immunology 2021Quote: ... then incubated for 45 min with constant shaking with “digest buffer” (RPMI; 10% FBS; 1mg/ml collagenase/dispase (Roche); 20μg/ml DNAse 1 (Sigma)) ...
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bioRxiv - Genomics 2019Quote: ... Nuclei pellets were resuspended in 1 ml nuclei permeabilization buffer (5 % BSA, 0.2 % IGEPAL-CA630, 1mM DTT and cOmpleteTM, EDTA-free protease inhibitor cocktail (Roche) in PBS ...
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bioRxiv - Immunology 2021Quote: B16F10 cells expressing ovalbumin (B16-ova, (Dieterich et al., 2017)) were cultured in DMEM supplemented with 10% FBS and 1.5 mg/ml G418 (Roche).
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bioRxiv - Immunology 2021Quote: Freshly collected feces were resuspended at 0.1mg/mL in 1X PBS containing EDTA-free Complete Mini Protease Inhibitor Cocktail Tabs (Roche) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Single-cell suspensions of the lung were prepared by digesting minced lung lobes for 30 min at 37°C with 0.2 U/ml Liberase TL (Roche) and 80 U/ml DNase (Life Tech ...
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bioRxiv - Immunology 2020Quote: ... Cells were isolated from equivalent areas of skin tissue (~30mm2) by mincing and digesting the tissue with 0.28 units/ml Liberase TM (Roche) and 0.1 mg/ml of Deoxyribonuclease I (Worthington ...
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bioRxiv - Neuroscience 2020Quote: The frozen half-brains were weighed and homogenized in 1 ml PBS containing complete protease inhibitor (Roche Applied Science) and 1 mM AEBSF (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... cells were collected by centrifugation (4,650 × g, 20 min) and resuspended in 15 ml of TNG buffer supplemented with Protease Inhibitor Cocktail (Roche). Cells from the SPA tagged protein cultures were mixed with the control WT or HA-tagged protein cultures at a 1:1 ratio ...
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bioRxiv - Immunology 2019Quote: Tissues from donor 390c were manually diced and <5.0g was added per Miltenyi C tube with 5mL tissue dissociation media (1x Liberase TL (0.13U/mL/DNase; Roche) and Benzonase nuclease (10U/mL ...
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bioRxiv - Immunology 2020Quote: ... The organs were chopped mechanically with scalpels and transferred into gentleMACS C tubes containing DMEM supplemented with 1μg/mL Collagenase D (Roche) and 25mg/mL DNase I (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... tissue was cut into pieces and digested for 15 minutes in a shaking water bath held at 37°C in a 0.5mg/ml solution of Dispase II (Roche) in PBS ...
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bioRxiv - Immunology 2021Quote: ... Right lung lobes were chopped finely and digested in pre-warmed RPMI1640 with ‘collagenase cocktail’ (0.625mg ml−1 collagenase D (Roche), 0.425mg ml−1 collagenase V (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... and the pellet was resuspended in 1-2 ml of lysis buffer (50 mM Tris-HCl, pH 8.0; 150 mM NaCl; protease inhibitor [Roche]), depending on the size of the cell pellet ...
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bioRxiv - Developmental Biology 2020Quote: ... The remainder of the fracture callus was minced and disassociated manually in Collagenase/Dispase (1 µg/ml, Roche, #10269638001) followed by digestion for 1 hour at 37 degrees ...
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bioRxiv - Developmental Biology 2020Quote: ... the tissue was minced and enzymatically digested in DMEM/F12 media containing 2 mg/ml Collagenase A (Roche #11088793001), 100 U/ml Hyaluronidase (Sigma #H3506) ...
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bioRxiv - Immunology 2021Quote: ... the tissues were finely chopped and transferred into 5ml polystyrene tubes () containing digestion buffer (5ml/sample) made with RPMI medium + Liberase TL (100μg/ml; Roche) + DNaseI (100μg/ml ...
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bioRxiv - Genomics 2021Quote: ... tissue was cut into 1mm x 1mm cubes and placed in 0.25mg/ml liberase TH (Roche Diagnostics, Indianapolis, USA) dissociation medium ...
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bioRxiv - Genomics 2021Quote: ... the tissue was chopped into 1mm x1mm cubes and placed in 0.25mg/ml liberase TH (Roche Diagnostics, Indianapolis, USA) dissociation medium ...
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bioRxiv - Microbiology 2022Quote: ... a 1% agarose gel block embedded with enterococci was treated with lysozyme solution (10 mg/mL; Roche, Basel, Switzerland) at 37 °C for 6 h ...
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bioRxiv - Immunology 2022Quote: ... T cells were isolated by dissociating tumor tissue in the presence of collagenase D (1 mg/ml) (Roche-Merck) for 10 min at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 ml urea buffer (8M urea, 1mM EDTA, 10mM TrisHCl pH 7.5, 1 mM PMSF, 1x Roche protease inhibitor) was then added and the sample was gently mixed ...
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bioRxiv - Molecular Biology 2022Quote: ... cells from 100 mL exponential phase cultures resuspended in 1.5 mL lysis buffer (buffer W with added cOmplete Mini protease inhibitor cocktail, Roche) were lysed by repeated sonication (10×20 s ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified supernatant was filtered through a 0.45 μM PES syringe filter and incubated with 10 mL of cOmplete His-Tag purification beads (Roche) for 1 h with gentle rocking ...
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bioRxiv - Biochemistry 2022Quote: ... The cell lysate was diluted to 0.8 mg/ml total protein concentration and 0.47% NP40 using dilution buffer (PBS plus protease inhibitor cocktail (Roche 04693159001) and precleared using Invitrogen™ Dynabeads™ MyOne™ Streptavidin T1 magnetic beads for 2-4 hours at 4°C during gentle rotation ...
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bioRxiv - Bioengineering 2022Quote: Mouse hearts were harvested from euthanized mice and enzymatically digested with collagenase A (1 mg/mL; Roche Life Science) and dispase (2.5 U/mL ...
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bioRxiv - Biophysics 2022Quote: ... Frozen bacteria containing full-length or ΔUBLΔUBA UBQLN constructs were resuspended in Buffer A (50 mM Tris pH 8, 1 mM MgCl2, 1 mM PMSF, and 0.2 mg/mL DNase I and Roche Complete Mini protease inhibitor cocktail) ...
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bioRxiv - Cancer Biology 2022Quote: ... and glands minced with surgical scissors before enzymatical dissociation for 1.5h in DMEM/F12 (1:1) supplemented with 2 mg mL−1 collagenase (Roche) + Gentamicin (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... finely chopped and incubated for 2 hours in FBS free DMEM media containing Liberase™ TL (0.25mg/ml; Roche) and DNAse I (0.5mg/ml ...
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bioRxiv - Genomics 2022Quote: ... the captured DNA was amplified for ten cycles using KAPA HiFi HotStart ReadyMix (6.25 ml, Roche, catalog number 7958935001) and purified in 1.8 x volume of Agencourt AMPure beads (Beckman Coulter ...
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bioRxiv - Genomics 2022Quote: ... Trim24 knockout TC-1 ES cells (background 129S6/SvEvTac) carrying an RMCE selection cassette (described previously82) were selected under hygromycin (250μg/ml, Roche) for 10 days ...
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bioRxiv - Neuroscience 2022Quote: ... 3 mL of Tris (pH = 8.5) and 3 tablets each of the protease/phosphatase inhibitors PhosStop and Complete Mini (Roche). Tissues were then lysed in Precellys Homogenizer Bertin (program 6 x 20s pulse ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were harvested by cell scraping into 2 ml of PBS with a protease inhibitor cocktail (Roche cOmplete 4693159001). The cell suspension was centrifuge at 200xg for 10 min ...
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bioRxiv - Physiology 2023Quote: ... enzymatic digestion was carried out for 20-25 min at 36 °C in the low-Ca2+ solution containing Liberase TH Research Grade (0.15 mg/mL; Roche) and elastase (0.5 mg/mL ...
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bioRxiv - Microbiology 2022Quote: ... Cells were lysed with lysis buffer (final concentration: 50 μg/ml lysostaphin, 0.8% NP-40, 1x protease inhibitor (Roche), 250 U/ml benzonase and 1 mM MgCl2 in PBS ...