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Citations for Roche :
5651 - 5700 of 5959 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... treated with 1 µL RNase A (Thermo) for 30 minutes followed by 1 µL Proteinase K (20 mg/mL, Roche) for 1 hour at 37°C ...
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bioRxiv - Physiology 2023Quote: FDB muscles were carefully dissected from the foot and placed into DMEM with 0.5-1mg/ml Collagenase A (Roche 10103578001). Fibers were digested at 37°C overnight ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and then subjected to a transcardial perfusion with calcium-free Tyrode’s solution containing protein phosphatase-inhibitors (+PPIs) (1 tablet PhosSTOP per 10 ml) (Roche 04906845001) followed by Zamboni’s fixative containing 4% paraformaldehyde and 0.2% picric acid in 0.1 M phosphate buffer pH 7.4 +PPIs ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed by sonication and French Press in purification buffer (50mM sodium phosphate buffer pH 7, 300 mM NaCl) supplemented with 5 ug/mL DNaseI (Roche), 5ug/mL RNAse (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2023Quote: ... For Salm protein purification from bacterial lysates (lisozima 0,5mg/mL, Imidazol 10mM and protease inhibitors cOmplete™ Protease Inhibitor Cocktail tablets from Roche) we used 3 rounds of 3 minutes sonication with 15 s on/off cycles ...
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bioRxiv - Biophysics 2023Quote: ... Pellets were resuspended in 7 ml of buffer A supplemented with the addition of 200 μl of protease inhibitor cocktail (one tablet (Roche), dissolved in 1 ml buffer A) ...
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bioRxiv - Microbiology 2023Quote: ... A total amount of 1010 bacteria were pelleted at 4°C and resuspended in 1 mL of buffer A (20 mM Tris-HCl pH 8, 200 mM MgCl2) supplemented with protease inhibitor cocktail (PIC, Roche) and lysozyme to a final concentration of 0.5 mg/mL ...
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bioRxiv - Biochemistry 2023Quote: ... SRA01/04 cell lysates were obtained using RIPA lysis buffer (Beyotime) supplemented immediately before use with 10 μl ml-1 phosphatase and protease inhibitors (PhosSTOPTM, Roche). The bicinchoninic acid assay (Beyotime ...
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bioRxiv - Neuroscience 2023Quote: ... microvessels obtained in the pellet were combined into a new conical sterile tube and further digested with collagenase-dispase (1 mg/ml, Roche) and DNase I (6.7 μg/ml ...
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bioRxiv - Immunology 2023Quote: ... Kidneys were homogenized using the Miltenyi gentleMACS Dissociator and digested for 20 minutes at 37°C in RPMI with 2 mg/mL collagenase D (Roche) and 0.1 mg/mL DNAse I ...
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bioRxiv - Cell Biology 2023Quote: ... Two brains were transferred to a pre-chilled 30-mL glass tube Wheaton homogenizer containing 5 mL of cold ‘solubilisation-plus’ buffer (solubilisation buffer with 1X COMPLETE protease inhibitor and 1X PhosSTOP phosphatase inhibitor (Roche)) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Genomic DNA was normalized to 20ng/ml and 100ng were used as template for amplification of barcodes using KAPA Hifi HotStart Ready Mix (Roche), applying the following PCR program ...
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bioRxiv - Immunology 2023Quote: ... tumor tissue was dissected into approximately 1– 5 mm3 fragments and digested with collagenase Type D (2 mg/ml; Roche) and DNase I (1 mg/ml ...
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bioRxiv - Bioengineering 2023Quote: ... were purchased from Lonza and cultured in endothelial media (VascuLife, Lifeline cell systems) on T75 or T150 flasks coated with 50 μg/ml rat tail collagen I (Roche). HUVEC were transduced to stably express cytoplasmic RFP (LentiBrite RFP Control Lentiviral Biosensor ...
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bioRxiv - Microbiology 2023Quote: ... coli cell pellets was carried out by resuspending pellets in 50 mL of lysis buffer (50 mM NaPO4, 300 mM NaCl, 20 mM imidazole, 10 mM 2-mercapoethanol, protease inhibitor (Roche), DNAse (Sigma) ...
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ER mediates spatial regulation of lysosome-endosome interactions via motion switch at junction sitesbioRxiv - Cell Biology 2023Quote: ... A total of ∼1×107 HEK293T cells expressing GFP-STARD3 were lysed with 1 mL lysis buffer supplemented with a protease inhibitor cocktail (cOmplete, Roche). Lysates were clarified by centrifugation at 12000 g for 60 min and were mixed with the beads coated with 2 mg GST/GST-VAPA-MSP for 60 min at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... 0.05% (v/v) Tween-20) supplemented with 10 μg/mL DNase I and 1 cOmplete protease inhibitor cocktail tablet (Roche) per L buffer ...
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bioRxiv - Neuroscience 2023Quote: ... pH 8.0, incubated in a hybridization buffer [(50% formamide, 5× SSPE, 0.1% SDS, and 1 mg/ml Yeast tRNA (Roche Diagnostics)] containing the Avidin solution (Vector Laboratories ...
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bioRxiv - Cell Biology 2022Quote: ... the cells washed twice with cold PBS and then scraped from the cell culture dishes in 1 mL PBS with cOmplete protease inhibitor by Roche. The cells were washed once with lysis buffer 1 (140 mM NaCl ...
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bioRxiv - Immunology 2023Quote: ... the common bile duct was clamped and the pancreatic duct was perfused with 3-5 mL solution of collagenase P in HBSS-1% HEPES (Roche). The pancreas was then harvested and transferred to a 50mL conical tube containing 5mL of collagenase P solution and kept on ice until all organs were collected ...
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bioRxiv - Immunology 2023Quote: ... 0.5 –1 L of the crude lysates were treated with 5 mg/mL each of DNase I and RNAse (Roche) for one hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Infected cells were selected 48 h after transduction with antibiotic supplemented media containing either 0.1 mg/mL Hygromycin B (Roche, H3274), 5 μg/mL Blasticidin S Hydrochloride (Gibco ...
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bioRxiv - Biochemistry 2023Quote: The cell pellets were re-suspended in 2 ml of mitochondrial isolation buffer (200 mM sucrose, 10 mM Tris HCl, 1 mM EGTA/Tris, protease inhibitor cocktail (Roche), pH7.4 (adjusted with 1M HEPES)) ...
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bioRxiv - Biochemistry 2023Quote: ... The pellet was resuspended in 30 mL of 40 mM Tris pH 8 supplemented with 1 tablet of EDTA-free protease inhibitor tablet (Roche), and 0.1 mM PMSF ...
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bioRxiv - Cell Biology 2023Quote: ... the cell monolayers were scraped into 10 ml KPBS (136 mM KCl and 10 mM KH2PO4, pH 7.25, containing protease (Roche, 1836153001) and phosphatase inhibitors (PhosSTOP Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... CVF samples were normalized to a protein concentration of 1 mg/mL using sterile PBS and EDTA-free protease inhibitor cocktail (Roche) was added ...
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bioRxiv - Immunology 2023Quote: ... were isolated from spleens of naïve WT C57BL/6 mice by cutting samples into small pieces and incubating at 37°C for 45 minutes with 1U/ml DNase I (Roche) and 10U/ml collagenase D (Roche ...
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bioRxiv - Immunology 2023Quote: ... Lung tissues were homogenized with a GentleMACS dissociator (Miltenyi) and digested with a cocktail of Liberase (70 μg/mL, Roche) and aminoguanindine (10mM ...
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bioRxiv - Physiology 2023Quote: ... The signal was then visualized by incubating the slides for 48 hours in a solution containing 0.33 mg/mL NBT (Nitro-blue tetrazolium chloride, Roche, 11383213001) and 0.165 mg/mL BCIP (5-Bromo-4-chloro-3-indolyl phosphate ...
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bioRxiv - Cell Biology 2023Quote: ... and the cell pellet was resuspended in 1 ml vPBS (PBS, 46 mM sucrose, 10 mM glucose) containing EDTA-free protease inhibitors (Roche). The washed cells were pelleted by centrifugation (750xg ...
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bioRxiv - Systems Biology 2024Quote: ... The lungs were perfused through the pulmonary artery and inflated via the airway with DMEM containing 1 mg/ml Collagenase/Dispase (Roche), 3 U/ml Elastase (Worthington) ...
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bioRxiv - Immunology 2023Quote: ... minced whole spleens and pancreatic lymph nodes (PancLN) were digested in basal RPMI 1640 supplemented with 25 ug/ml Liberase TM (Roche) and 25 ug/ml DNase I for 20 min under agitation at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Cells were harvested by centrifugation at 7000 xg for 15 minutes and were lysed in 10 ml PBS supplemented with protease inhibitor (cOmplete, Mini, EDTA-free, Roche). NatT was purified by immunoprecipitation using Anti-FLAG magnetic beads (Merk ...
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bioRxiv - Biophysics 2023Quote: Cell lysate powder from 12 L of cell culture was resuspended in 300 mL of room temperature eIF4G Lysis Buffer supplemented with 2 EDTA-free protease inhibitor tablets (Roche), 20mM imidazole ...
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bioRxiv - Cancer Biology 2023Quote: ... the skin from euthanized neonatal mice of indicated genotypes was incubated with 250 units/mL dispase (Roche, Catalog No. 04942078001) in KSFM medium (Thermo Fisher ...
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bioRxiv - Developmental Biology 2023Quote: ... Larvae were dissociated using a combination of enzymatic disruption using Liberase (Roche, 05 401 119 001, 5 μg/mL final), DNaseI (Sigma ...
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bioRxiv - Biophysics 2024Quote: ... Tumor spheroids were digested into single cell suspensions using 0.028 Wünsch units/ml of Liberase TM Research Grade (Roche, Mannheim, Germany) and 20 μg/ml of DNase I in L-15 with 10 % CS for 1 hour at 37 °C and 250 RPM on an orbital shaker ...
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bioRxiv - Bioengineering 2024Quote: ... livers were perfused at low flow for about 10 min with digestion buffer (low-glucose DMEM supplemented with 1 mM Hepes) containing freshly added Liberase (32 μg/ml; Roche). Digestion was stopped using isolation buffer (low-glucose DMEM supplemented with 10% FBS) ...
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bioRxiv - Plant Biology 2024Quote: ... A total of 5 mL of either test buffer supplemented with 1 mM phenylmethylsulfonyl fluoride and one protease inhibitor tablet (Roche) was then to resuspend the fine powder ...
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bioRxiv - Cell Biology 2024Quote: ... The minced hearts were transferred to digestion buffer (20mM BDM, 1.5mg/ml Roche Collagenase/Dispase enzyme mix in Leibovitz L15 medium)(Roche, 10269638001)(cellgro ...
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bioRxiv - Cell Biology 2024Quote: ... dissected TA muscles were first digested with 10ml muscle digestion buffer (DMEM containing 1% penicillin/streptomycin, 0.125mg/ml Dispase II (Roche, 04942078001), and 10mg/ml Collagenase D (Roche ...
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bioRxiv - Genomics 2024Quote: ... were digested for 1 hour at 37°C under 110 rpm shaking conditions in DMEM supplemented with 2 mg/mL collagenase D (Roche) and 0.4 mg/mL Dispase (Gibco) ...
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bioRxiv - Physiology 2024Quote: Dissected tips of digits and foot pads of mice were minced and incubated in 0.25mg/mL liberase TL (Roche, 5401119001) for 45 min at 37 °C with pipetting every 10min ...
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bioRxiv - Biophysics 2024Quote: ... 50 uL of 0.2 nM Cy3-GAF-DBD in Imaging Buffer (50 mM NaCl, 50 ug/mL BSA (Roche 10711454001), 0.05% NP40 (Sigma I8896) ...
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bioRxiv - Biochemistry 2024Quote: ... For lysis the pellets are thawed and resuspended in 2 ml of lysis buffer containing benzonase and cOmplete protease inhibitors (Roche), and processed as described for small scale expression in E ...
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bioRxiv - Cancer Biology 2024Quote: ... in buffer (10 mM Tris-Cl pH8.0, .25 mM EDTA, 100mM NaCl, 0.5% SDS 1mg/ml proteinase K) (Roche Cat #03508838103) at 55 °C for 4 hours to remove proteins ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with a 20-fold excess of unlabeled oligonucleotide with the same sequence as the labeled one (to prevent reannealing) and 1 µl of Proteinase K (18 mg/ml) (Roche). After deproteination for 30 min at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: Xenograft OS tumors were harvested and microdissected 12 d after 143B OS cell inoculation and digested with collagenase type I and II (1 mg/ml; Roche) digestion for 45 min ...
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bioRxiv - Cell Biology 2024Quote: ... The tissue was then washed twice in PBS with antibiotic and antimycotic and digested with 2mg/ml collagenase P (Roche) in DMEM (Gibco ...
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bioRxiv - Plant Biology 2024Quote: ... Total 2.5 g powder of each sample was resuspended in 30-ml 1% TFA with 250 μl protease inhibitor cocktail (Roche) and then centrifuged at 12,000 ×g for 20 min at 4℃ ...