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Citations for Merck :
3851 - 3900 of 3939 citations for 1 1 Dimethylsila 11 Crown 4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and equal amounts of proteins were separated by SDS-PAGE (10% or 4-20% gradient gels) and transferred to Immobilon-P membranes (Merck-Millipore). After chemiluminescence detection ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were blocked in 10% normal goat serum in TBS-T for one hour at room temperature and then incubated in the following primary antibodies suspended in blocking buffer overnight at 4°C: pS129 (El-Agnaf laboratory, 1µg/ml 27) and 5G4 (MABN389, Merck, 2µg/ml) combined with either MGO-aSyn (1µg/ml ...
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bioRxiv - Plant Biology 2021Quote: ... the washed seedlings were transferred into TetSee X solution (15% Na-deoxycholate, 25% urea, 10 % glycerol, 5% 2’2-thiodiethanol [Merck, Product No. 166782], 1% Triton X-100) and kept for 3 days at 4°C with daily changes of the TetSee X solution ...
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bioRxiv - Neuroscience 2020Quote: ... Cell lysis of all samples was performed after two washes with ice-cold PBS by incubating cells for 20 min in ice-cold 1% Triton X100 in PBS with protease inhibitor cocktails (Merck, Calbiochem set III, Cat. No. 539134), subsequent thorough scraping and sonication.
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bioRxiv - Neuroscience 2020Quote: ... Bacteria were resuspended in (50mM HEPES, 250mM NaCl, 10% glycerol plus 1% Triton X-100, supplemented with protease inhibitors (Merck, Calbiochem set III, Cat. No. 539134) and subsequently sonicated ...
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bioRxiv - Microbiology 2020Quote: ... First strand cDNA preparations were diluted 1:10 with RNase-free water and subjected to RT-qPCR using SYBR Green JumpStart Taq ReadyMix (Merck Life Science UK Ltd., Gillingham, UK), with primers specified in Table S2 ...
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bioRxiv - Cell Biology 2023Quote: ... non-cholesterol precursor sterols and 5a-cholestanol, 50 μg 5α-cholestane (Serva, Heidelberg, Germany) (50 μl from a stock solution of 5α-cholestane in cyclohexane [Merck KGaA, Darmstadt, Germany]; 1 mg/ml), and one μg epicoprostanol (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... At 5 hpf embryos were placed into 10 cm Petri dishes containing E3 medium (control) and E3 medium with 1 μM VPA (Sigma-Aldrich, P4543; Merck Life Science Srl, Milan, Italy) for 24 or 48 h ...
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bioRxiv - Neuroscience 2024Quote: ... The membranes were later incubated at room temperature for 1 hour with 5% fat free milk in DPBS (D1408, Merck Life Science, Milan, Italy, pH 7.4) and subsequently incubated with the primary antibody overnight at 4 °C (Table 1) ...
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bioRxiv - Microbiology 2020Quote: ... Expanded and chopped gels were stored at 4°C in ddH2O immobilized prior to imaging on PDL-coated glass chambers (Merck, 734-2055).
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bioRxiv - Microbiology 2020Quote: ... HPLC analysis was performed using a Waters e2695 separation module (Waters) equipped with a LiChrospher 100 RP-18 column (250 × 4 mm, φ = 5 μm; Merck Millipore) at 50°C ...
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bioRxiv - Microbiology 2021Quote: Embedding in Lowicryl K4M (Chemische Werke Lowi, Waldkraiburg, Germany) was carried out on Vero cells fixed either in 4% formaldehyde (Merck, Darmstadt, Germany) or in 2% glutaraldehyde at 4°C ...
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bioRxiv - Immunology 2020Quote: Survival was determined by assessing the fraction of vital cells at defined time points during culture by flow cytometric exclusion of 4’,6-Diamidin-2-phenylindol (DAPI) (Merck, Darmstadt, Germany) and Annexin V-APC (BD Biosciences ...
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bioRxiv - Neuroscience 2022Quote: ... spherical assembloids were collected in a PCR tube and immediately incubated in BBB working medium with 10 μM 4 kDa dextran-FITC (FD4; Merck, cat. #46944) or 10 μM fluorescently labeled human transferrin (Tf488 ...
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Farnesyltransferase inhibition overcomes the adaptive resistance to osimertinib in EGFR-mutant NSCLCbioRxiv - Cancer Biology 2022Quote: ... fixed with paraformaldehyde 4 % solution for 10 min and stained with a solution containing PBS - 0.5% crystal violet (Sigma-Aldrich/Merck, ref: C3886) – 25% methanol for 10 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... The cortices were homogenized mechanically in PBS by pipetting up and down with a 5mL plastic pipette, centrifuged (500 x g, 7 min, 4 °C) and resuspended in DMEM (Sigma-Aldrich, Merck, Switzerland) containing 5% FCS (Biochrom ...
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bioRxiv - Biochemistry 2020Quote: ... concentration and buffer exchange to TE with an Amicon Ultra-4 centrifuge filter unit (30 kDa cut-off, Merck Millipore, Cat#UFC803024).
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bioRxiv - Physiology 2021Quote: ... Membranes blocked with 5% non-fat milk in PBS-T or Tris-Buffered Saline TBS (TBS-T) (RT, 60min) and immunoblotted using the following primary antibodies (4°C, overnight): rabbit anti-pSmad1/5/8 (Merck Millipore, AB3848), rabbit anti-pSmad2 (Cell Signaling ...
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bioRxiv - Physiology 2021Quote: ... Lysates were centrifuged (16,000 g, 10 min, 4°C) and supernatants measured for protein content using infrared spectroscopy (Merck Millipore, Darmstadt, Germany). Lysates were then reduced ...
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bioRxiv - Immunology 2021Quote: ... These fractions were then pooled and buffer exchanged using SnakeSkin™ dialysis tubing (10 MWCO, Pierce Cat#68100) followed by further dialysis and concentration using Amicon Ultra-4 10kDa centrifugal units (Merck, Cat# UFC801096). The clinically relevant mAbs tested here were in-house produced versions of ...
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bioRxiv - Synthetic Biology 2021Quote: ... Protein containing fractions were pooled and buffer exchanged using SnakeSkin™ dialysis tubing (10 MWCO, Pierce Cat#68100) followed by further dialysis and concentration using Amicon Ultra-4 10kDa centrifugal units (Merck, Cat# UFC801096), as described previously (5).
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bioRxiv - Biophysics 2020Quote: Upon deposition the samples were washed 2 times 15 minutes with PBS prior to overnight fixation with 4% paraformaldehyde (PFA, Merck, Darmstadt, Germany). Washing steps and incubation took place under agitation at 4 °C unless stated otherwise ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant was filtrated through a filter paper (Whatman No. 4, Whatman plc, Kent, UK) and a membrane filter (MF-milliporeTM 0.22 μm, Merck KGaA, Darmstadt, Germany). The solution was cleaned up through a Waters Sep-Pak C18 column (Waters ...
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bioRxiv - Microbiology 2022Quote: ... Slides were mounted in CitiFluor™ CFM3 mounting medium (proSciTech, Australia) containing 3 μg/uL 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI; Merck, Germany) to stain nucleic acids ...
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bioRxiv - Molecular Biology 2023Quote: ... and loaded in 4–12% NuPAGE Bis-Tris gel (Fisher, 10247002) for subsequent electrophoresis and transference to nitrocellulose membrane (Merck-Sigma, GE10600003). Precipitated RNA was analyzed by autoradiography and FLAG-PTBP protein levels were measured by western blot.
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bioRxiv - Biochemistry 2023Quote: ... cells were harvested by centrifugation at 5,000 × g and 4°C for 5 min and lysed with BugBuster Protein Extraction Reagent (Merck Millipore, Burlington, MA) prepared in 20 mM sodium phosphate buffer (pH 7.4 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... rat slices were exposed to 3 μM dihydroethidium (DHE) while in humans’ sections it was added 4 μM DHE (Merck, Darmstadt, Germany) for 30 minutes at 37ºC 51 ...
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bioRxiv - Neuroscience 2022Quote: ... Mice were administered a lethal dose of sodium pentobarbital and transcardially perfused with Ringer’s solution followed by paraformaldehyde (PFA, 4 %, Merck Millipore, Darmstadt, Germany) in 125 mM phosphate buffer (PB) ...
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bioRxiv - Neuroscience 2022Quote: ... Sonicated sample was diluted with 3.5 ml PBS and concentrated to 500 μl by diafiltration using an Amicon® Ultra-4 Centrifugal Filter Unit with 30 KDa cutoff (Merck UFC803024) to remove traces of sarkosyl ...
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bioRxiv - Neuroscience 2022Quote: ... and concentrated to 300 ul by diafiltration using an Amicon® Ultra-4 Centrifugal Filter Unit with 30 kDa cutoff (Merck UFC803024) to remove the potentially unincorporated dye ...
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bioRxiv - Neuroscience 2023Quote: GNAO1+/G203R#2 cells at the stage of neuronal rosettes (30 day of differentiation) were fixed in 4% paraformaldehyde solution (Merck Life Sciences) for 15 min at room temperature and washed thrice with 1X PBS (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... The flow-through was further concentrated from 10 ml volume in an Amicon® Ultra-4 Centrifugal Filter 50 kDa unit (Merck Millipore) and stored at 4 °C ...
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bioRxiv - Plant Biology 2024Quote: The following chemicals were used in the DEEP-Clear (Pende et al., 2020) adapted version to plant tissues: PFA (paraformaldehyde) (CAS no. 30525-89-4, Merck, http://www.merck.com/), xylitol (CAS no ...
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bioRxiv - Synthetic Biology 2024Quote: ... Protein containing fractions were pooled and buffer exchanged using SnakeSkin dialysis tubing (10 MWCO, Pierce Cat#68100) followed by further dialysis and concentration using Amicon Ultra-4 10kDa centrifugal units (Merck, Cat# UFC801096), as described previously (44).
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bioRxiv - Microbiology 2024Quote: ... the same dilutions were also asdded to Eosin Methylene Blue Agar and MacConkey Agar both supplemented with cefotaxime (4 mg/L) (Merck, Darmstadt, Germany) for the Extended-spectrum beta-lactamase phenotype (37) ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue homogenisation and western blotting was performed with 50 μg of total protein as previously described.15 Membranes were incubated overnight with gentle agitation at 4 °C with the MSH3 antibody (Merck MSH3MAB(1F6)) in 5% milk PSB-T (phosphate buffer saline (PBS) ...
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bioRxiv - Bioengineering 2023Quote: ... at 40,000 xg for 20 min at 4°C and the supernatant was filtered through a 0.45 µm Millex® syringe filter (Merck, Darmstadt, Germany). Clarified lysate was loaded onto a nickel (II ...
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bioRxiv - Biochemistry 2023Quote: Total cell lysates of HEK293 cells stably expressing C-terminally Flag M2-tagged SIRT4 were incubated overnight at 4°C with anti-Flag M2 agarose beads (Merck, Darmstadt, Germany). The beads were washed three times with washing buffer (50 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were washed in 1x PBS and subsequently mixed with 100 μL 4% SDS (Carl Roth GmbH, Karlsruhe, Germany) and 2% beta-Mercaptoethanol (Merck, Darmstadt, Germany) in PBS ...
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bioRxiv - Bioengineering 2023Quote: ... Conditioned medium was then centrifuged at 1500 rpm for 5 min and concentrated 30x times in Amicon® Ultra-4 Centrifugal Filter Unit with 30 kDa MWCO cut-off (Merck Millipore). Concentrated conditioned medium was then filtered using 0.2 μm filters and frozen until use.
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bioRxiv - Developmental Biology 2022Quote: ... 32.8 mM 20:4 and 60 mM 22:6) and pipetted dropwise into N2 media containing 3 mM fatty acid-free BSA (Merck, Cat. #A8806) at 37°C with constant stirring to obtain final concentrations of 3 mM 18:0 ...
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bioRxiv - Biochemistry 2022Quote: Collected samples from the first main peak were concentrated by ultrafiltration on 10K Amicon® Ultra-4 Centrifugal Filters Units (Merck Millipore) in a swinging-bucket rotor 4 000 g ...
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bioRxiv - Biochemistry 2022Quote: ... The TEV cleaved complex was concentrated to around 500 μl using a 4 ml Amicon® Ultra centrifugal filter concentrator (Merck Millipore) with a membrane molecular weight cut off 10 kDa ...
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bioRxiv - Cell Biology 2023Quote: ... The blots were developed in a solution of nitroblue tetrazolium chloride (NBT) and 5-brom-4-chlor-3-indoxylphosphate (BCIP; Merck, Darmstadt, Germany) for 5–30 min at RT ...
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bioRxiv - Biophysics 2023Quote: ... containing 0.3□mM biotin and concentrated at +4 °C to 25 μl volume using Amicon ®Ultra 10 kDa MWCO centrifugal filters (Merck Millipore).
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bioRxiv - Molecular Biology 2024Quote: ... a total of 0.4–0.5 μg of plasmid and dsRNAs were transfected into BmN4 cells (4–6 × 104 cells per glass bottom 35 mm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche), and the cells were fixed 5–6 days later ...
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bioRxiv - Neuroscience 2024Quote: ... Homogenized tissue was centrifuged at 16,000 g for 45 min at 4 °C and the remaining supernatant was spun in 3 kDa cut-off Amicon® centrifugal filter tubes (Merck Millipore) at 14,000 g for 90 min at 4 °C ...
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bioRxiv - Plant Biology 2024Quote: In some of the experiments the samples were exposed to 5 mM concentration of the phosphatase inhibitor NaF (CAS#: 7681-49-4; Merck, Darmstadt, Germany) that prevents the dephosphorylation of LHCII and therefore also the reversal of light acclimation from state 2 to state 1 (Rintamäki et al ...
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bioRxiv - Microbiology 2024Quote: ... the SN was concentrated by ultrafiltration at 4000□g and 4°C using 100 K Amicon® Ultra-15 centrifugal filters (Merck Millipore). The concentrated SN was filtered through a 0.45-µm-pore-size filter ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... a mixture of the antibody constructs with a 10-fold molar excess of DFO*-NCS (5 mg/mL in DMSO) (ABX, Radeberg, Germany) was incubated ON at 4 °C with gentle shaking (Eppendorf ThermoMixer, Merck, Darmstadt, Germany). The DFO*-derivatized constructs were purified by SE-HPLC under the same conditions as described above.