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Citations for Merck :
3451 - 3500 of 3939 citations for 1 1 Dimethylsila 11 Crown 4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The pre-cleared lysates were then incubated overnight at 4°C with polyclonal rabbit anti-TipR antibodies (1:400 dilution) (Kirkpatrick and Viollier, 2014) or monoclonal rabbit anti-HA antibodies (1:250 dilution) (Clone 114-2C-7, Merck Millipore). ...
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bioRxiv - Microbiology 2023Quote: Washed DPI-treated VBNC Mtb cells were resuspended with fresh Dubos broth and the suspension was diluted to 1:10 with Dubos broth with 0.1% (w/v) BSA containing adenylyl cyclase inhibitor SQ22536 (Merck, Darmstadt, Germany) or protein kinase inhibitor H89 (Abcam ...
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bioRxiv - Biochemistry 2023Quote: Hippocampal cells (5.104 cells/well) were treated with 10 and 30 μmol L-1 H2O2 solutions [from H2O2 stock solution 30% (Merck, Germany), into supplemented neurobasal medium] fresh made ...
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bioRxiv - Immunology 2023Quote: ... and PBMCs (1 × 106 cells per well) were seeded in 96-well plates in RPMI supplemented with 1% PNS and 10% AB human serum (Merck, UK) and stimulated with SARS-CoV-2 specific peptides pools ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transfected with 1-2 µg of pcDNA5 FRT/TO plasmids containing the gene of interest using GeneJuice transfection reagent (Cat#70967, Merck Millipore) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... For fluorescent imaging the 30 µM protein solutions were supplemented with 300 nM (100:1 molar ratio) NTA-Atto 550 (Sigma Aldrich, Merck). Movies were acquired at 37°C with a PerkinElmer UltraVIEW VoX microscope equipped with Yokogawa CSU-X1 spinning disk head and Nikon TiE microscope body with an Apo 60x/1.49 numerical aperture (NA ...
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bioRxiv - Bioengineering 2023Quote: An immunofluorescence assay was performed in cryosections of rd10 animals using the following antibodies: rabbit polyclonal cone arrestin (1/5000, Merck Millipore), mouse monoclonal rhodopsin (1/1000 ...
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bioRxiv - Immunology 2023Quote: ... A549 cell were incubated with lentiviral supernatants diluted 1:20 (v/v) in culture medium containing 10 µg/ml polybrene (Merck Millipore) and centrifuged at 805xg at 20 °C for 30 min ...
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bioRxiv - Genomics 2023Quote: ... 20 µL of frozen cells were lysed by a 3-min incubation at 100°C in 5% boiling SDS with 2x final concentration of Protease Inhibitor Cocktail Set 1 (Merck Chemicals). After centrifugation (5 min 15000g) ...
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bioRxiv - Neuroscience 2023Quote: Place 100 μl trypsin and 100 μl collagenase (per 2 animals) in a 35 mm dish: 2 mg ml-1 Trypsin (Merck - 85450C) and 682 U ml−1 Collagenase in Ca2+ and Mg2+ free medium
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bioRxiv - Plant Biology 2023Quote: A barrel of a 1 mL needleless syringe is filled with 800 µl of an aqueous (double-distilled water) ‘infiltration solution’ that contains 1 mM of the fluorophore pyranine (pKa in aqueous solution: 7.3; Merck, Darmstadt, Germany). The water serves for extracting the apoplastic solutes ...
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bioRxiv - Immunology 2024Quote: ... The cells were washed three times in PBS 1× and incubated for 1 hour at 37°C with 40 μL of mouse anti-DENV complex monoclonal antibody MAB8705 (Merck Millipore) diluted 1:200 in PBS 1× ...
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bioRxiv - Immunology 2024Quote: ... For analysis of splenic myeloid populations spleens were dissociated using scissors in 1.2 mL of digestion media containing 1 mg/mL collagenase D (Merck Life Sciences) and 0.1 mg/mL DNase I (Merck Life Sciences ...
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bioRxiv - Cell Biology 2024Quote: ... and the pellet containing the cells was resuspended in 1 mL PBS supplemented with protease inhibitor (cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail; Merck). The cells were lysed by 20 passages through a bead homogenizer (16 µm bead ...
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bioRxiv - Cell Biology 2024Quote: ... and the pellet was resuspended in 1 mL PBS supplemented with protease inhibitor (cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail; Merck). The cells were lysed by approx ...
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bioRxiv - Cancer Biology 2024Quote: ... in T-BST for 1 h at room temperature or Re-Blot Plus Strong Antibody Stripping solution for 15 min at room temperature (Merck, #2504). Quantification of protein bands was performed using ImageJ analysis software (release 1.53c).
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bioRxiv - Genetics 2024Quote: ... The cells were first arrested at G1 using 3.0 µg mL-1 α-factor at 30°C (Merck, Cat. No. T6901). After 90 min ...
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bioRxiv - Plant Biology 2024Quote: ... Blotted proteins were then visualised using goat anti-rabbit HRP secondary antibody (Thermo Fischer Scientific) at dilution 1: 900 and ECL Western HRP substrate (Merck Millipore).
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bioRxiv - Pathology 2024Quote: Quarter kidneys stored at −80°C were placed in 200 µl RIPA buffer (150 mM NaCl, 10 mM Tris HCl, 1% Na deoxycholate, 0.1% SDS) with protease inhibitor (Merck, cat# 05892791001). To this ...
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bioRxiv - Systems Biology 2024Quote: ... Media was designed based on the Delft composition with a glucose concentration of 9 g/L and the addition of 1 mL/L of Antifoam 204 (Merck, Germany). The bioreactors were inoculated at an OD600 of 0.1 ...
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bioRxiv - Immunology 2024Quote: ... deparaffination was followed by 1-hour incubation in room temperature with the primary antibody (monoclonal rat, MAB386; Merck Millipore, Darmstadt, Germany) diluted 1:800 in dilution buffer (Agilent Dako ...
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bioRxiv - Microbiology 2024Quote: ... a red tetrazolium salt that turns yellow when cleaved to formazan by mitochondrial dehydrogenases (WST-1 Assay Protocol for Cell Viability, MERCK®). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... The chromatin pellet was then digested in 100 μl of water supplemented with 1 μl of Benzonase (25–29 units, Merck Millipore) for 15 min at 37°C in a thermomixer at 1,400 rpm.
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bioRxiv - Molecular Biology 2024Quote: ... 200 μl of Protein powder solutions (1 mg/ml) were added to 3 cm NG plates with 10 µM 5-FU (Merck, #F6627). After the plates had dried ...
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bioRxiv - Cancer Biology 2024Quote: ... The shRNA mediated knockdown of the respective EWSR1::ETS fusion oncogenes was induced by addition of 1 μg/mL dox (Merck, Germany) to the cell culture medium ...
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bioRxiv - Cell Biology 2024Quote: ... Thawed Matrigel was diluted 1/50 in cold E8 media and was then added to the cell culture dishes (Merck, #Z755923). Coated dishes were incubated at room temperature for 1 hour and used immediately or kept in the fridge for later application.
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bioRxiv - Neuroscience 2024Quote: ... fish were briefly rinsed in water and embedded in 1% agarose (Carl Roth, Germany) in 1.8 ml vials (Nunc CryoTube Vials, Merck (Sigma-Aldrich), Germany) ...
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bioRxiv - Microbiology 2024Quote: ... ParT (S48C)-His6 and ParT (Q271C)-His6 aliquots were supplemented with 1 mM tris (2-carboxyethyl) phosphine hydrochloride (Merck; cat# C4706) before flash-freezing in liquid nitrogen.
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bioRxiv - Microbiology 2024Quote: SFPQ and FLAG RIPs were performed in TREx-BCBL-1 RTA cells using EZ-Magna RIP RNA binding Immunoprecipitation Kit (Merck Millipore) as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies were incubated for 90 minutes at room temperature: anti-rabbit Alexa Fluor® 647 (1:800; Merck Millipore, #AP187SA6), anti-mouse Alexa Fluor® 488 (1:800 ...
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bioRxiv - Molecular Biology 2024Quote: ... and 300 µg of protein was then incubated with 1 µg of a mouse anti-53BP1 antibody (1μg/ml, Merck millipore, MAB3802) O/N at 4 °C ...
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bioRxiv - Bioengineering 2024Quote: ... The activated microgels were then resuspended in 3 mL of PBS and 50 µL of 100 U mL−1 thrombin (Merck T1063) and stirred at room temperature overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 million cells per mL were suspended in 1 mL of a 1.2% sodium alginate solution (Merck, Saint-Quentin-Fallavier, France). Beads were formed by dripping the cell suspension into a sterile 100 mM CaCl₂ solution (VWR ...
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bioRxiv - Synthetic Biology 2024Quote: ... LCC and Fast PETase. Single colonies (E. coli XJb (DE3)) were cultured in 1 mL of overnight autoinduction media (Merck, USA) supplemented with 50 µg/mL kanamycin ...
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bioRxiv - Biophysics 2024Quote: ... and resuspended in 1 mL PBS supplemented with protease inhibitor and protease inhibitors (cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail; Merck). The cells were lysed by 20 passages through a bead homogenizer (16 µm bead ...
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bioRxiv - Biochemistry 2024Quote: ... These sections were then treated with 1 mL of artificial cerebrospinal fluid which composed of 120mM NaCl (Merck Cat. No. S9888), 2.5 mM KCl (Merck Cat ...
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bioRxiv - Cell Biology 2024Quote: ... SRM medium was supplemented with same concentrations of LDN and SB and adding 1 μM of Smoothened Agonist (SAG; Merck Millipore), 2 μM of Purmorphamine (Tocris Bioscience) ...
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bioRxiv - Developmental Biology 2024Quote: ... the lung distal regions were cut into small pieces and dissociated in 5 mL of enzyme mixture (0.125 mg/mL Collagenase, Merck, C9891; 1 U/mL Dispase, Thermo Fisher Scientific, 17105041; 10 U/mL DNAase, Merck, D4527) at 37°C for 1 hour with rotation ...
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bioRxiv - Developmental Biology 2024Quote: ... All ingredients of the “Amino acid mixture” (Supplemental Table 1) were weighed out and added to a glass beaker containing ultrapure water (Milli-Q Water Purification System, Merck Millipore). Tyrosine was separately pre-dissolved in 2.8 M KOH ...
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bioRxiv - Biophysics 2020Quote: mamM CTD gene from Magnetospirillum gryphiswaldense MSR-1 (UniProt Q6NE57 residues 215-318) was previously cloned into pET28a(+) vector (Novagen, Merck Biosciences, Germany)22 ...
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bioRxiv - Cell Biology 2020Quote: ... GFP (1:2000) (Faix et al., 2001) or mouse monoclonal antibody against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:1000; #CB1001-500UG, Merck (Darmstadt, Germany)) and anti-fascin antibody 5E2 (undiluted hybridoma supernatant) ...
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bioRxiv - Neuroscience 2020Quote: ... individual COs were transferred to an Eppendorf tube containing CMM medium with 1:100 of virus containing medium and 5µg/ml Polybrene (Merck; TR-1003-6) and incubated over-night ...
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bioRxiv - Immunology 2021Quote: ... wells washed with 1 X PBS and incubated with 50 µL of incomplete media containing 5 µM Laurdan (Avanti® Polar Lipids, Merck, USA) for 30 min at 37°C ...
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bioRxiv - Genomics 2020Quote: ... C57BL/6 ESCs were transduced with the lentivirus at a multiplicity of infection (MOI) ranging from 0.1 to 1 with 5 μg/μL polybrene (Merck Millipore, Cat# TR1003G). From day three onward (e.g ...
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bioRxiv - Genomics 2021Quote: ... The cloned shRNAs against the respective EWRS1-ETS fusion oncogenes was induced by addition of 1 µg/ml dox (Merck, Darmstadt, Germany) to the cell culture medium ...
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bioRxiv - Bioengineering 2021Quote: ... and the remaining skin was cut in strips of approximately 4 cm length and 1 mm width and finally incubated in 10 ml skin digestion solution (2.0 U/mL dispase D4693, Merck KGaA, in PBS-) at 4°C overnight ...
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bioRxiv - Biochemistry 2021Quote: ... After adding 1 drop of bovine thrombin (Biomed, Lublin, Poland) to 2 drops of fibrinogen (1mg/ml; Sigma-Aldrich, St. Louis, MO; Merck KGaA) the cells were entrapped within the fibrin clots ...
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bioRxiv - Neuroscience 2021Quote: ... the slices were processed with 1% H2O2 in phosphate buffer to deactivate the intrinsic peroxidase and then incubated with an anti-c-Fos antibody (1:10,000, rabbit; Merck KGaA, Darmstadt, Germany) in 10% normal goat serum in phosphate-buffered saline with 0.3% Triton X-100 (PBS-X ...
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bioRxiv - Neuroscience 2020Quote: ... glial glutamate transporter GLT1 (guinea pig, polyclonal, synthetic peptide from the C-terminus of rat GLT1, Merck, AB1783, AB_90949, dilution 1:1,000), GFP (chicken ...
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bioRxiv - Cell Biology 2021Quote: ... Blots were then reprobed with monoclonal anti-β-actin-peroxidase clone AC-15 antibody (a3854, Merck, Saint Louis, MO, dilution 1:5000) in TBS/T for 90 minutes ...