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Citations for Merck :
3601 - 3650 of 3939 citations for 1 1 Dimethylsila 11 Crown 4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... The 1,2-Distearoyl-sn-glycero-3-phosphocholine (DSPC, CAS: 816-94-4) was sourced from Merck (Darmstadt, Germany). Cholesterol (Chol ...
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bioRxiv - Biophysics 2024Quote: ... The urified protein was concentrated using an Amicon® Ultra-4 centrifugal filter unit – 3000 NMWL (Merck-Millipore).
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bioRxiv - Cell Biology 2024Quote: ... and mounted on grease-free slides using Mowiol- based mounting medium (12% Mowiol 4-88 (Merck, 81381-250G), 30% glycerol ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was clarified by ultracentrifugation at 55,000 g for 60 min at 4°C and filtered through 0.45 µm (Durapore Merck) filters ...
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bioRxiv - Biochemistry 2024Quote: ... with the additional step that lysis was preceded by 4 h treatment with 10 μM MG132 (Merck, 4693159001) and 10 μM PR619 (Bio-Techne ...
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bioRxiv - Cell Biology 2020Quote: ... Beads were then washed 2x in Tween wash buffer (5 mM Tris-HCl [Sigma], 0.5 mM EDTA [AppliChem], 1 M NaCl [Merck], 0.05 % Tween-20 [Sigma]) and 1x in H2O ...
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bioRxiv - Microbiology 2021Quote: ... the cell layers were washed again with PBS and then lysed for 10 min with PBS containing 1% Elugent (Merck Millipore, Darmstadt, Germany) and 0,0625% Antifoam B (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2020Quote: Fibrinogen stock solutions were prepared by dissolving 1 g of human fibrinogen lyophilized from 20 mM citrate pH 7.4 solutions (Merck 341576, >90% clottable proteins) into distilled water as recommended by the manufacturer (final volume of app ...
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bioRxiv - Biochemistry 2020Quote: ... The imidazole of the elution fraction was removed until the concentration of imidazole was below 1 mM using Amicon® centrifugal filters (0.5 ml size, GlnA1: 30 kDa and GlnK1: 10 kDa) (Merck KGaA, Darmstadt, Germany). His6-sP26 was purified as described above with the following additional steps ...
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bioRxiv - Biophysics 2020Quote: ... functionalized with either 100 μg/mL poly-D-lysine overnight for microglia cells or with 0.2 mg/mL fibronectin (FC010, Merck, 1:5 in PBS) for 2h at 37°C for fibroblasts ...
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bioRxiv - Synthetic Biology 2021Quote: ... the diet for both the transgenic and wild-type strains was supplemented with CuSO4•5H2O (1 mM, Merck, CAS-no: 7758-99-9). Flies were reared in a controlled environment room at 25.0 °C ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 900 µl supernatant was transferred to a new tube and incubated with 25 µl (1/2 * OD in µl) of mouse-anti-FLAG antibody (clone M2, Merck/Sigma-Aldrich #F1804) at 4 °C for 45 min (rocking) ...
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bioRxiv - Cell Biology 2021Quote: ... Free floating brain sections and fixed astrocyte cultures were immunostained with the following antibodies (Table S2): Mouse anti-NeuN (1:100, Merck Millipore, Darmstadt, Germany), Rabbit anti-GFAP (1:200 ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated with secondary antibody for 1 hour at room temperature and proteins were detected using chemiluminescent HRP substrate (Merck-Millipore, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Biophysics 2022Quote: ... The PDMS particles were generated by vortex-mixing a solution of 1 g PDMS (10:1 w/w, base/curing agent) dispersed in 10ml of 2%w/v poly(ethylene glycol) monooleate (Merck Chemicals GmbH, Germany) aqueous solution ...
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bioRxiv - Biophysics 2022Quote: ... was washed three times in Tris-buffer (pH 7.48) and resuspended in 1 %w/v Pluronic® F-127 (Merck Chemicals GmbH, Germany) Tris-Buffer.
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bioRxiv - Microbiology 2021Quote: ... aspergillus minimal media (AMM) [33] were added in pre-wetted 96-well MultiScreen Filter Plates HTS (1 μm glass fiber filter, Merck Millipore, Darmstadt, Germany) and incubated for 15 min at 37°C with either 25 μL PBS or 25 μL [Fe]TAFC solution (control – uptake block ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in blocking solution (5% bovine serum albumin in PBST) for 60 min at room temperature and then incubated with anti-CB1R (Merck Millipore, 1:1000) in blocking solution overnight at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... PDGCLs were lentivirally transduced with the MISSION® shRNA vector pLKO.1-puro-CMV-Turbo green fluorescent protein (TurboGFP)_shnon-target (#SHC016, Sigma, part of Merck, Darmstadt, Germany) for cytosolic TurboGFP expression ...
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bioRxiv - Biochemistry 2022Quote: ... was added and incubated for 1 min at room temperature with gentle shaking (100 mM stock was prepared from fresh H2O2 Merck 30% w/w).
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bioRxiv - Biochemistry 2022Quote: ... the resulting solids were resuspended in 1 mL of UP-water and desalted using centrifugal filtration (Merck, Amicon® Ultra 10 kDa filters) at 14,000 rcf for 10 minutes at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Testes were washed three times (10 min each) with PBS-T and incubated with anti-H4ac (Merck Millipore #06-598, 1/500 dilution), anti-Histones (Millipore #MABE71 ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked in 5% BSA TBS-T or 5% skim milk TBS-T and incubated overnight at 4°C with the following primary antibodies diluted in 5 % BSA or 5% skim milk dissolved in TBS-T: pSer293-PDH (1:1,000) (Merck Millipore, Cat. no. ABS204), PDH (1:1,000 ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue sections from the in vivo experiment were incubated with a rabbit primary antibody for GluR2/3 (Merck Millipore; AB1506; 1:200) overnight and then with a secondary goat anti-rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Biochemistry 2024Quote: ... CHAPS was added to the dialyzed sample to concentration of 1% after which the MSP was concentrated to 20-40 mg/ml in centrifugal filter (Merck, MWCO 30 kDa), flash frozen and stored at -80°C.
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bioRxiv - Biophysics 2024Quote: ... The phages were then purified with two different protocols: Protocol 1: Phage lysates were filtered through 0.22 µm polysulfone membrane filters (Merck Millipore, Billerica, MA, USA) and were used for subsequent purification and concentration by cross-flow filtration using Sartorius Hollow fiber cartridges ...
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bioRxiv - Molecular Biology 2023Quote: ... Relative levels of pFlareG were calculated for each condition using GAPDH as a housekeeping gene and normalized relative to FLAG-PTBP2 protein levels immunoprecipitated checked by western blot using α-FLAG M2 antibody (1:500; Merck-Sigma, F3165) and IRDye 800RD (1:10000 ...
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bioRxiv - Neuroscience 2023Quote: ... Four small insertions were made and the retina was flat mounted on a filter paper annulus (1 mm inner radius; 0.8 µm hydrophilic MCE MF-MilliporeTM membrane filter, Merck Millipore Ltd., Tullagreen, Ireland). The retina was then mounted photoreceptor cell side up on a perforated 60-electrode MEA chip (60pMEA200/30iR-Ti ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were blocked using 2% normal goat serum and stained using the following primary antibodies in blocking solution overnight: anti-Ng2 (1:100, rabbit pAb, AB5320 Merck Millipore, Burlington, MA), anti-beta III tubulin (1:100 ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 mM EDTA) with 1 µl lyticase (17 U/ µl in 10 mM KPO4 pH 7, 50% glycerol, Merck >2000 U/mg L2524), heated to 50°C for 2 min before addition of 40 µl molten CleanCut agarose (Bio-Rad 1703594) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the frozen cell suspension was thawed and incubated with end-over-end mixing together with 1 mg/mL lysozyme (Merck KGaA, Darmstadt, Germany) for 30 min (all purification steps at 4 °C of on ice) ...
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bioRxiv - Plant Biology 2023Quote: ... The sections were then incubated for 1 h with 700 µL of a 10% (v/v) DMSO/3% (v/v) IGEPAL® CA-630 (Merck, Darmstadt, Germany) in MTSB ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then treated with antibodies against FLAG (Monoclonal ANTI-FLAG® M2 antibody produced in mouse; Merck CAT# F3165; 1:200 dilution) or CSNK1D (Anti-Casein Kinase 1 delta/CSNK1D antibody [AF12G4] ...
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bioRxiv - Plant Biology 2024Quote: ... or standards (gallic acid in the range of 0–80 µg ml-1) were mixed with 200 µl of 10% Folin-Ciocalteu’s phenol reagent (Sigma-Aldrich, Merck, Burlington MA, USA) and incubated at 25°C in the dark for 5 min ...
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bioRxiv - Cancer Biology 2024Quote: ... The following day the membrane was washed with T-PBS (0.1% tween) (15 min x 3 times) and appropriate secondary antibody: (i) anti-rabbit antibody conjugated with peroxidase enzyme (Merck Sigma, Burlington, MA) was dissolved in 5% BSA in T-PBS (0.1% tween ...
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bioRxiv - Neuroscience 2024Quote: ... sections were blocked 1h in 10% donkey serum in PBST and incubated overnight with primary antibodies for anti-GFAP (1:250, Merck Life Science, Hpa056030) and anti-MAP2 (1:200 ...
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bioRxiv - Genomics 2024Quote: ... with the following primary antibodies: goat anti-IL1RN (RD Systems Cat # AF280NA) in 1:1000 in blocking solution and mouse anti-β actin (MERCK Cat # A1978) and rabbit anti-vinculin (Cell Signaling Cat #18799 ...
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bioRxiv - Molecular Biology 2024Quote: ... The plate was washed three times with TBS-C blocking solution and incubated with goat-anti mouse IgG-HRP (A4416, Merck; dilution 1/10,000) added in TBS-C blocking solution and incubated during 1 h at RT ...
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bioRxiv - Immunology 2024Quote: ... 1-26 amino acids) was amplified by PCR using corresponding primers (Supplementary table 1) and subcloned into pET-32a (+) plasmid (Merck Millipore, Darmstadt, Germany). After confirmed by sequencing ...
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bioRxiv - Biochemistry 2024Quote: ... The QconCAT was precipitated and resuspended in 30 μL 25 mM ammonium bicarbonate with 0.1% (w/v) RapiGest™ SF surfactant (Waters, UK) and protease inhibitors (Roche cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail, Merck, UK).
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bioRxiv - Developmental Biology 2021Quote: ... after which they were washed three times in PBST and blocked overnight at 4°C in 10% serum (Merck) in PBS/0.3% TritonX100 ...
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bioRxiv - Molecular Biology 2020Quote: ... and incubated for 16h at 4°C with 2µL α-H3K27me3 antibody (#07-449; Merck Millipore, Burlington, MA, USA) or α-H3K4-me3 antibody (Rabbit monoclonal antibody (#04–745 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with the nucleus DNA stain 4′,6-diamidino-2-phenylindole (DAPI) (1μg/ml D9542-10MG, Merck Life Science UK Ltd ...
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bioRxiv - Microbiology 2020Quote: ... RNA pellet thus obtained by centrifugation at 16,000 × g for 20 min (4°C) was washed using 70% ethanol (Merck) and resuspended in nuclease-free water after air drying ...
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bioRxiv - Physiology 2020Quote: ... Proteins in the conditioned medium were concentrated up to 1.2ug/ul of total protein using cellulose membrane Ultra-4 Centrifugal Filter Unit of 3KDa cut-off (Merck) as per the manufacturer instructions.
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bioRxiv - Biophysics 2020Quote: ... fluorescently-labeled and biotinylated H57-scFV were again concentrated with 10kDa Amicon®Ultra-4 centrifugal filters (UFC801024, MERCK) and stored in PBS supplemented with 50% glycerol at −20°C ...
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bioRxiv - Biophysics 2021Quote: ... cells were washed 2x 4 min in PBS and subsequently overlaid with 0.1 mg/ml collagen IV (C7521, Merck) and placed at 4°C overnight (ON) ...
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bioRxiv - Cancer Biology 2021Quote: Cells grown on coverslips were washed with PBS and incubated 15 min in 4% formaldehyde (formalin solution buffered at pH 6.8, Merck). Cells were washed once with 50 mM ammonium chloride (Carl Roth ...
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bioRxiv - Biochemistry 2021Quote: ... cells were trypsinized and seeded for selection in complete media supplemented with 4 μg/mL puromycin (Calbiochem, Merck Millipore). All cell lines were passaged 3 times before use and kept in culture supplemented with 2 μg/mL puromycin and/or stored in liquid nitrogen.
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bioRxiv - Cell Biology 2021Quote: ... and then equal volumes were concentrated 50-fold using Amicon Ultra-4 centrifugal filter units (Merck Millipore, Watford, U.K.). For analysis of cell-associated proteins ...