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Citations for Merck :
3551 - 3600 of 3939 citations for 1 1 Dimethylsila 11 Crown 4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Actin was detected with monoclonal mouse antibody coupled with horseradish peroxidase (HRP; 1:75,000; A3854, Sigma-Aldrich, Merck, Saint-Quentin Fallavier, France). Membranes were exposed to the appropriate peroxidase-conjugated secondary antibodies (1:5000 goat anti-guineapig IgG A108P or 1:10000 sheep anti-mouse IgG A5906 ...
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bioRxiv - Bioengineering 2024Quote: ... The extracts were prepared by incubating the formulations (1 ml, prepared as detailed above) in 3.3 ml DMEM HG medium (Merck, Cat. No. D6429) for 24 h at 37°C at a surface-to-volume ratio of 3 cm2/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... Chromatin was then sheared either by passaging 10 times through a 27G needle attached to a 1 ml syringe or by the addition of Benzonase® Nuclease (Merck, UK). Needle-sheared nuclear fractions were centrifuged at 13,000 x g for 10 minutes (4°C ...
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bioRxiv - Biophysics 2024Quote: ... The membrane pellet was resuspended in extraction buffer (25mM MOPS-NaOH, 150mM NaCl, 50mM EDTA, 10mM DTT, 0.1 mg/ml lysozyme, 1% lauroylsarcosine (Merck, Cat. No. L5777), pH 7.0 ...
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bioRxiv - Cell Biology 2024Quote: ... HaCaT KCs were cultured for 4 hours after seeding in DMEM supplemented with 10% FBS (HyClone) and 1× antibiotic–antimycotic mixed stock solution (Nacalai Tesque) on 3.0-µm-pored cell culture inserts (Merck KGaA, Darmstadt, Germany). The inserts were pre-coated with CELLstart (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... The extracted 212Pb in 0.1 M HCl obtained from the emanation generator was adjusted to pH 5-6 with 5 M sodium acetate (Merck, Darmstadt, Germany) and mixed with TCMC-mAbs with specific activities of 1-50 MBq/mg ...
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bioRxiv - Molecular Biology 2021Quote: ... Slices were then stored at 4°C in a PBS-solution containing 0.05% of Proclin (Merck, Darmstadt, Germany) until further processing.
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bioRxiv - Biophysics 2020Quote: ... Eluted fractions containing monomeric H57-scFV were concentrated with 10 kDa Amicon® Ultra-4 centrifugal filters (MERCK), treated with a 3C protease (71493 ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.2 buffer were concentrated to 10 mg/ml using the Amicon Ultra-4 Centrifugal Filter Unit (Merck/Millipore ...
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bioRxiv - Molecular Biology 2020Quote: ... The Bruce-4 cell line was maintained in DMEM supplemented with 15% FCS and ESGRO (Merck Millipore, UK) on a 0.1% gelatine-coated dish at 10% CO2.
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bioRxiv - Molecular Biology 2021Quote: ... treated with PBS-formaldehyde (4%) for 20 mins at room temperature and stained with crystal violet stain (Merck). After extensive washing ...
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bioRxiv - Pathology 2022Quote: ... trans-cardial perfusion was performed into the mice and rats with PBS followed by 4% paraformaldehyde (PFA, Merck). The kidneys were harvested and the samples were post-fixed in 4% PFA at 4°C for 90 min ...
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bioRxiv - Bioengineering 2022Quote: ... Sliced sections were permeabilized and blocked with 0.1% Tween-20 and 4% donkey serum (Merck Millipore, Burlington, MA) in Tris-buffered saline ...
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bioRxiv - Cell Biology 2022Quote: ... coli cells using 0.2 mM IPTG for 4 hours at 30 °C and purified on flag beads (Merck) and eluted using flag peptide buffer (200 µg/ml flag peptide in 10 mM Tris-HCL ...
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bioRxiv - Cell Biology 2022Quote: ... lysates were incubated overnight under rotation at 4°C with ANTI-FLAG® M2 Affinity Gel beads (Merck) to pull down FLAG-containing SNAP-GLP-1R ...
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bioRxiv - Neuroscience 2021Quote: ... Protein wet transfer was performed overnight at 4°C using an Immobilon®-FL PVDF membrane (Merck™). Next day ...
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bioRxiv - Biochemistry 2021Quote: ... were incubated overnight at 4°C with rotation with 20 µg/ml anti-FLAG M2 antibody (Merck, F3165) and 50 µl/ml of pre-washed Pierce magnetic protein A/G beads (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... 2 μL lipid extract was injected on a LiChroCART 250–4 LiChrospher® Si 60 (5 μm) (Merck) maintained at 25 °C ...
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bioRxiv - Immunology 2020Quote: ... fixed with paraformaldehyde (4% w/v (Applichem) in phosphate-buffered solution (PBS)) and used for Giemsa-staining (Merck) to calculate the number of perivascular neutrophils ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates were harvested in 4°C RIPA buffer with protease inhibitor (cOmplete, EDTAfree Protease Inhibitor Cocktail, Merck) and homogenised by sonication for 30 sec ...
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bioRxiv - Immunology 2021Quote: ... Frozen tissues were sectioned to a thickness of 5 μm and fixed with 4% paraformaldehyde (Merck, Kenilworth, NJ). Slides were incubated with 1% BSA in PBST for 1 h to block the non-specific antibody binding ...
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bioRxiv - Immunology 2023Quote: ... Fractions containing monomeric and labeled 14.4.4 scFVs were concentrated with Amicon Ultra-4 centrifugal filters (10 kDa cut off, Merck) to arrive at a protein concentration between 0.2 mg ml-1 and 1 mg ml-1 ...
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bioRxiv - Molecular Biology 2023Quote: ... pooled according to purity and afterwards concentrated using Amicon Ultra-4 centrifuge filters (30 kDa cutoff, Merck Millipore). The purified octamers were validated by SDS-PAGE ...
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bioRxiv - Biophysics 2023Quote: ... Peak fractions were pooled and concentrated in an Amicon Ultra-4 Ultracell 30kDa centrifugal filter (Merck-Millipore #UFC803024). Aliquots were snap frozen and stored at −80 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Nuclear translocation of FLAG-ER-SAMD1 was induced by adding 200 nM 4-OHT (Merck; 68392-35-8) for 24 h.
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bioRxiv - Neuroscience 2023Quote: ... and the slices were placed on Millicell membranes (3–4 slices per membrane, 0,4 µm Millicell, Merck Millipore). Slices were cultured in DMEM/F-12 with GlutaMax ...
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bioRxiv - Pathology 2023Quote: ... The samples were resuspended in 10 µl 4 M GdCl (Guanidinium Hydrochloride) in 50 mM HEPES (#G3272, Merck) and sonicated in a water bath sonicator for 10 min ...
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bioRxiv - Neuroscience 2023Quote: ... and then protein was loaded into Superdex 200 and concentrated using Amicon Ultra-4 (3K cut off, Merck). The labeling percentage was calculated according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... Peak fractions were pooled and concentrated in an Amicon Ultra-4 Ultracell 30kDa centrifugal filter (Merck-Millipore #UFC803024). Aliquots were snap frozen and stored at −80 °C ...
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bioRxiv - Biophysics 2023Quote: ... Peak fractions were pooled and concentrated in an Amicon Ultra-4 Ultracell 30kDa centrifugal filter (Merck-Millipore #UFC803024). Aliquots were snap frozen and stored at −80°C ...
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bioRxiv - Cell Biology 2023Quote: ... sections were fixed with 4% PFA and titrated to pH 5 using a buffer containing sodium acetate (Merck) and sodium tartrate-dehydrate (Merck) ...
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bioRxiv - Microbiology 2024Quote: ... Products were separated on a LiChroCART 125-4 RP-18 end-capped 5 µm column (Merck, Darmstadt, Germany) with a solvent system of methanol and phosphoric acid (0.1% ...
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bioRxiv - Molecular Biology 2022Quote: ... The Swi2C-Swi5 complex was concentrated using an Amicon Ultra-4 (30 k) centrifugal filter (UFC803008, Merck Millipore). After cleavage ...
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bioRxiv - Molecular Biology 2022Quote: ... The Swi2CS-Swi5 complex was concentrated using an Amicon Ultra-4 (10 k) centrifugal filter (UFC801008, Merck Millipore).
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bioRxiv - Molecular Biology 2022Quote: ... The Swi2S-Swi5 complex was concentrated using an Amicon Ultra-4 (50 k) centrifugal filter (UFC805008, Merck Millipore), applied to a 5 mL HiTrap Heparin column ...
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bioRxiv - Cell Biology 2022Quote: ... and concentrated at 4 °C approximately 300 times using a 10 kDa Centricon Plus-70 centrifugal unit (Merck Millipore ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: HepaRG cells were washed twice with 100 μl PBS pH 7.4 and fixed with paraformaldehyde solution (4% in PBS pH 7.4, Merck) for 40 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... cell culture medium was removed and cells fixed with 100μl of 4% para-formaldehyde (PFA) (Merck, London UK) for 20 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... pH 4.2) and 4-methylumbelliferyl-2-acetamido-2-deoxy-b-D-glucopyranoside (2 mM; #474502, Sigma-Aldrich/Merck). The reaction was stopped by 5 volumes of glycine and Na2CO3 ...
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bioRxiv - Cell Biology 2022Quote: E10.5 embryos were fixed in 4% PFA/PBS for 1.5 h followed by incubation in 15% sucrose (Merck)/PBS for 2 h and overnight incubation in 30% sucrose/PBS ...
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bioRxiv - Biochemistry 2023Quote: ... at 4 °C for 30 s and concentrated in a 100 kDa Amicon Ultra centrifugal filter (Merck Millipore), which was equilibrated with 1% GDN in IP buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... concentrated with an Amicon Ultra-4 centrifugal filter with a 5 kDa molecular weight cutoff (UFC8003, Merck Millipore), snap frozen and stored at -80 °C either directly (used for cryo-EM ...
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bioRxiv - Cell Biology 2023Quote: ... resuspended in PBS containing 0.2μM DAPI and maintained at 4°C until measurement by IFC or mounted in Mowiol (Merck) on a microscopy slide.
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bioRxiv - Immunology 2023Quote: ... Tissue samples (200 mg) were digested in 4 ml of EMSURE ISO nitric acid 65% solution (Merck, #1.00456.2508) using MARS 6 microwave extraction system ...
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bioRxiv - Plant Biology 2024Quote: ... IZEs were cultured on a medium with 2,4-D and treated with different yucasin (Merck; #23711-26-4) concentrations (0 ...
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bioRxiv - Immunology 2024Quote: ... Cell supernatants were harvested 5 days post-transfection by centrifuging for 10 min at 4,000 g and 4 °C and sterile filtering through a 0.45 μm filter followed by a 0.22 μm filter (Merck). cOmplete mini EDTA-free protease inhibitor cocktail was added to the supernatants at 1 mg/mL (Roche).
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bioRxiv - Biochemistry 2024Quote: ... Coverslips were rinsed in distilled water and mounted using Mowiöl (12 ml of 0.2 M Tris buffer pH 7.5, 6 ml distilled water, 6 g glycerol, 2.4 g Mowiöl 4-88, Merck Millipore). Samples were imaged immediately or within the next 48 h ...
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bioRxiv - Biochemistry 2024Quote: ... 500 μM of the protease inhibitor 4-(2-aminoethyl)-benzolsulfonyfluorid hydrochloride (BioChemica) and 5 U/mL-benzonase (Merck) were added (final concentrations) ...
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bioRxiv - Biophysics 2024Quote: ... concentrated using 4 mL and 0.5 mL 30 kDa MW cut-off centrifugal filters (Amicon Ultra, Merck Millipore) to concentrations >1 µM ...
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bioRxiv - Biochemistry 2024Quote: ... Then the mixtures were concentrated to about 400 μM of protein concentration using Amicon Ultra-4 (Merck Millipore). Crystallization screening was performed using different commercially available crystallization screen by the sitting drop vapor-diffusion method at 4°C and 20°C ...