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Citations for Merck :
751 - 800 of 1623 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The proteins were separated via 15% SDS-PAGE and transferred to nitrocellulose membranes (Merck-Millipore). Western blot analyses were then conducted using PilA- ...
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bioRxiv - Neuroscience 2022Quote: ... 25,000 cells were plated on ELISpot IP Filter Plates (PVDF membrane, 0.45 um, Merck; MSIPN4550) and fixed at 50°C for 1 h before treatment with 1 ug/ml proteinase K (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... the cells on the transwell membrane were permeabilized in 0.5% Triton-X (Merck, Darmstadt, Germany) in PBS for ten minutes at 4 °C and then washed in 0.1% Tween20 in PBS (PBST ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was pre-hybridized for 30 minutes in Perfect Hyb plus (Merck, Sigma Aldrich) at 50°C in rotation ...
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bioRxiv - Microbiology 2024Quote: ... we had to switch to a 1 µm PTFE membrane (Omnipore, Merck Millipore, Darmstadt, Germany). Filters were stored on dry ice or −80 °C until DNA extraction.
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bioRxiv - Molecular Biology 2024Quote: ... Protein was transferred to an Immobilon®-FL PVDF membrane (Merck Millipore, Billerica, MA, USA) and blocked in Licor blocking buffer for 120 minutes (Licor ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were resolved via SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membrane (Merck, GE10600023) with a wet electroblotting system (Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: ... the protein was filtered through an Ultrafree-MC centrifugal device with hydrophilic PTFE membrane (Merck) at 10,000 x g ...
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bioRxiv - Molecular Biology 2023Quote: ... membranes were rinsed in water prior to immunodetection with 3,3’,5,5’-tetramethylbenzidine (Sigma-Aldrich/Merck).
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bioRxiv - Cell Biology 2023Quote: ... The membranes were washed with TBS 5× 5 min and developed using BCIP/NTP (Merck). The reaction was stopped using MQH2O ...
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bioRxiv - Biochemistry 2023Quote: ... membranes were probed with an anti-poly-ADP-ribose (1:1000 in 10% milk, Merck) as well as anti- APLF and anti-GST as loading controls.
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bioRxiv - Cancer Biology 2023Quote: ... membranes were stained with Ponceau S and subsequently blocked in 4% BSA (A8022, Sigma/Merck) in 1x PBS-Tween (137 mM NACl ...
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bioRxiv - Microbiology 2023Quote: ... virus stocks were concentrated on a 100 KDa cellulose membrane (Amicon Ultra-15, Merck Millipore) and rinsed three times successively with PBS ...
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bioRxiv - Biophysics 2023Quote: ... The obtained solutions were purified using Amicon centrifugal devices (Amicon Ultra-0.5,100 kDa membrane, Merck). The solution of ssDNA-SWCNTs was added to the filtration device and rinsed eight times (at 4,000 × g for 2 min ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were transferred to PolyVinyliDene Fluoride 0.45 µm membranes (Immobilon-Fl, Merck Millipore, Darmstadt, Germany). Membranes were saturated 1 hour with a Tris-Buffered Saline blocking solution (TBS blocking solution ...
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bioRxiv - Neuroscience 2023Quote: ... The proteins were transferred to FL-PVDF membranes (cat. No. IPFL00010; Merck Millipore; Massachusetts, USA). The membranes were incubated with ms anti-Y1-R (1:500 ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blotted with the following antibodies: anti-human PSEN1-CTF (MAB5643; Merck Millipore); anti-PEN2 (D6G8 ...
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bioRxiv - Biochemistry 2023Quote: ... membranes were blocked with 5% BSA in TSMT (20 mM Tris; 150 mM NaCl, Merck; 1 mM CaCl2 ...
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bioRxiv - Cell Biology 2023Quote: ... The resolved proteins were then transferred to the PVDF membrane (0.45 μm, cat. IPVH00010, Merck) as described previously123 ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were separated using SDS-PAGE and transferred to polyvinylidene difluoride membranes (Immobilon-FL, Merck). The membranes were blocked for 30 min with Blocking-One or Blocking-One P (Nacalai Tesque ...
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bioRxiv - Biochemistry 2023Quote: ... and concentrated using a Centricon with a 10 kDa cutoff membrane (Merck Millipore, Burlington, MA). The concentrated sample was then purified by size exclusion chromatography using a HiLoad 26/600 Superdex 200 column (Cytiva) ...
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bioRxiv - Physiology 2023Quote: ... The resolved proteins were then transferred to the PVDF membrane (0.45 μm, cat. IPVH00010, Merck) as described previously107 ...
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bioRxiv - Genomics 2023Quote: ... and finally concentrating viral particles on 0.1 μm pore filters (Merck, Millipore (Durapore PVDF Membrane)) ...
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bioRxiv - Immunology 2023Quote: ... Protein bands were visualized by treating the membrane with Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore ...
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bioRxiv - Microbiology 2023Quote: ... for 90 minutes at 100 V and blotted onto Immobilon-FL PVDF membranes (Merck Millipore). The transfer was performed a constant voltage of 30 V for 30 minutes using semi-dry transfer system ...
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bioRxiv - Physiology 2023Quote: The resolved proteins were then transferred to the PVDF membrane (0.45 μm, cat. IPVH00010, Merck) as described previously23 ...
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bioRxiv - Pathology 2024Quote: ... Proteins were then transferred from the gel onto to a polyvinylidene fluoride membrane (Merck Millipore) via electroblotting at 120 V for 60 minutes in transfer buffer containing 20% methanol ...
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bioRxiv - Microbiology 2024Quote: ... the separated proteins were semi-dry blotted onto an Immobilon-FL PVDF-Membrane (Merck, #IPFL00010) at a constant voltage of 30 V for 30 min ...
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bioRxiv - Genetics 2024Quote: ... Proteins were then separated using 10% SDS-PAGE and transferred to PVDF membranes (Merck Millipore). PVDF membranes were then blocked by 5% BSA ...
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bioRxiv - Neuroscience 2024Quote: The isolated peripheral nerve endings were collected on the Isopore polycarbonate membrane filters (Merck, GTTP04700). The excess supernatant was vacuum-filtered ...
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bioRxiv - Molecular Biology 2024Quote: ... Membrane fractions were buffer-exchanged to PBS with Amicon Ultra 2-mL filters (Merck Millipore) and purified via FPLC with a Ni2+ column (Thermofisher).
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bioRxiv - Microbiology 2024Quote: ... The proteins in the gel were transferred onto polyvinylidene difluoride membranes (Merck Millipore, Billerica, MA). After protein transfer ...
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bioRxiv - Pathology 2024Quote: ... Separated proteins were transferred onto polyvinylidene difluoride membranes (Immobilon-P; Merck Millipore, Burlington, MA, USA). The membranes were reacted with the following primary antibodies ...
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bioRxiv - Genetics 2024Quote: ... Proteins separated by 10% SDS-PAGE were transferred onto a PVDF membrane (Immobilon-E, Merck) and blocked in 5% milk in Tris-Buffer Saline (TBS ...
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bioRxiv - Cell Biology 2024Quote: ... Protein lysates were separated by SDS-PAGE gels and transferred to PVDF membranes (Merck, IPVH00010). Membranes were stained with Ponceau-S ...
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bioRxiv - Neuroscience 2024Quote: ... and transferred to polyvinylidene fluoride (PVDF) membranes (0.45 μm pore size; Merck Millipore Ltd., Ireland) at 385 mA for 1 hour ...
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bioRxiv - Cell Biology 2024Quote: ... The suspension was concentrated by vacuum filtration onto a 0.45 µm membrane filter (Merck, HVWG04700), which was was placed onto an agar plate and the yeast paste was scraped using a pipette tip ...
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bioRxiv - Microbiology 2024Quote: ... 10ml of the sonication liquid was filtered aseptically using a 0.2µm nitrocellulose membrane (Sartorius, Merck). This membrane was then placed into a 50ml falcon tube containing 20ml of nutrient broth (Oxoid ...
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bioRxiv - Physiology 2024Quote: ... membranes were blocked in 5 % milk (w/v) in TBS-T (200 mM Tris (Merck), 1.36 mM NaCl (Merck) ...
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bioRxiv - Cell Biology 2024Quote: ... by SDS-PAGE followed by transferring onto methanol-activated Immobilon-PSQ PVDF transfer membrane (Merck). Membrane was blocked with 5% skimmed milk in Tris-buffered saline plus 0.1% Tween-20 (TBS-T ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were further washed with PBST three times and developed with a chemiluminescent substrate (Merck).
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentration of each lysate was determined using a BCA assay (Merck, 71285).
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bioRxiv - Cell Biology 2022Quote: Wound healing assays were performed in Corning® 6-well plates (Merck, UK). HDF and MSC were individually labelled with cell tracking dyes eBioscience™ CFSE (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Protein extracts were quantified using Direct Detect® Assay-free Cards (Merck; DDAC00010) or Pierce Bicinchoninic Bcid (BCA ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Cell viability assays were carried out using WST-1 Cell Proliferation Reagent (Merck). A498 cells were plated onto clear 96-well plates at 4,000 cells per well and left to adhere for 24 hours ...
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bioRxiv - Biochemistry 2023Quote: ... Assays were quenched using 100 μL 5 M H2SO4 (Merck, Rahway, United States). Ethylene and ethane were detected via gas chromatography using the gas chromatograph (GC ...
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bioRxiv - Cancer Biology 2023Quote: Cell viability was also assessed with MTT assay (Millipore, Merck Life Science, Germany). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... and the protein concentration was determined by bicinchoninic acid (BCA) assay (Merck Millipore) and by UV-visible spectroscopy using an ε of 24 mM⁻¹·cm⁻¹ at 280 nm ...
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bioRxiv - Physiology 2023Quote: ... and protein concentration in the supernatant was determined by BCA assay (Merck B9643). Finally ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cell viability assay was performed by propidium iodide (PI) (P4864, Merck, Gillingham, UK) staining followed by flowcytometry analysis [29] ...