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Citations for R&D Systems :
3151 - 3200 of 3214 citations for 8 1 Piperazinyl imidazo 1 2 a pyrazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... cells were harvested and transferred to a 24 well plate and cultured in the presence of rhuIL-2 (10 U/mL, R&D System) to expand the T cells ...
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bioRxiv - Microbiology 2023Quote: ... between 0.5-1.5×106 splenocytes were plated into 96-well u-bottom plates containing 10 ng/mL recombinant mouse IL-2 (R&D Systems). Samples were stimulated with PepMixTM Pan-Poxviridae peptide pool (JPT ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed three times with 2% PBS-BSA and incubated with secondary anti-goat IgG (H+L) APC-conjugated antibody (F0108, R&D systems) in 2% PBS-BSA for 20 minutes at room temperature at 0.05 µg/106 cells for ACE2-A549 cells or at 0.1 µg/106 cells for Vero E6 cells ...
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bioRxiv - Molecular Biology 2023Quote: ... TSP2 concentrations in LX-2 cell lysates were determined using enzyme-linked immunosorbent assays following the manufacturer’s instructions (Quantikine® ELISA, #DTSP20, R&D Systems) (16) ...
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bioRxiv - Immunology 2023Quote: ... was pre-incubated with either 10 µl biotinylated SARS-CoV-2 spike protein (3 nM final concentration; R&D Systems cat# BT10549), or with 10 µl biotinylated irrelevant antigen (3 nM final concentration ...
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bioRxiv - Neuroscience 2023Quote: Adherent human iPSCs were grown to near confluence before the differentiation to heterogenous spinal/hindbrain neurons was initiated by changing the media for two days to media d1-2 (200 nM LDN193189 [Tocris, 6053], 10 µM SB431542 [Tocris, 1614], 25 ng/mL SHH-C25II [R&D Systems, 464-SH-025] ...
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bioRxiv - Microbiology 2023Quote: ... separated and blotted onto 0.2 μm nitrocellulose as above and incubated overnight at 4°C in blocking buffer supplemented with 2 μg/ml polyclonal sheep IgG anti-LL-37 (R&D Systems). Membranes were washed in wash buffer (PBS with 0.05% Tween ...
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bioRxiv - Immunology 2023Quote: ... Unpurified transient transfection supernatant samples (50 µl) were pre-incubated with biotinylated SARS-CoV-2 spike protein (R&D Systems cat# BT10549) (50 µl ...
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bioRxiv - Microbiology 2022Quote: ... were obtained from healthy human blood donors after preparing blood monocyte monolayers and culturing for 2 days in medium containing macrophage colony-stimulating factor (R&D Systems) as described previously (56 ...
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bioRxiv - Immunology 2022Quote: ... The single memory B cells were cultured in complete RPMI 1640 medium supplemented with IL-2 (10 U/ml, R&D Systems), IL-21 (10 U/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... The lower compartment contained 500 μl of either cancer cell line derived conditioned medium or control media (serum free medium; 2% serum control; CCL19 (20 ng/mL, R&D Systems). The PBMCs were allowed to migrate from the upper chamber to the lower one for 3 hours ...
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bioRxiv - Bioengineering 2024Quote: ... CAR T cells for the initial challenge day 0 imaging experiment were stained with the following antibody mix in FACS buffer (PBS 1x + 2% FCS + 2mM EDTA): anti-RQR8 mAb clone QBEND10-APC (R&D Systems), anti-CD4 mAb clone VIT4-VioBlue (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2024Quote: ... cells from re-challenge plates were stained with the following antibody mix in FACS buffer (PBS1x + 2% FCS + 2mM EDTA): anti-RQR8 mAb clone QBEND10-APC (R&D Systems), anti-CD4 mAb clone VIT4-VioBlue (Miltenyi Biotec) ...
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bioRxiv - Bioengineering 2024Quote: MDA-MB-231 spheroids were formed starting from a culture of 10,000 cells per spheroid mixed with their medium and a 2% v/v Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract (BioTechne, R&D systems) solution in 96-well round-bottom plates (Corning Costar Ultra-Low Attachment Multiple Well Plate) ...
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bioRxiv - Cancer Biology 2024Quote: A pool of plasma from 2 to 4 mice was loaded on the membrane of the Proteome Profiler Mouse XL cytokine Array (ARY028, R&D systems) following manufacturer’s recommendations ...
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bioRxiv - Immunology 2024Quote: ... then maintained in RPMI-1640 (ATCC) + 10% FBS + 100 U/mL P/S + 30 U/mL of recombinant human IL-2 (R&D Systems). For the pilot experiment in which CD4 cells with a defined CAR BC were spiked into CD8 cells (Figure 1D-F) ...
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bioRxiv - Immunology 2024Quote: ... All primary cells were maintained in RPMI-1640 (ATCC) + 10% FBS + 100 U/mL P/S + 30 U/mL of recombinant human IL-2 (R&D Systems) at 37°C in 5% CO2.
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bioRxiv - Immunology 2021Quote: ... Human T cells were also cultured under conditions where 5 µg/ml neutralizing anti-IL-2 antibody (R&D Systems, Minneapolis, MN, USA) or 10 ng/ml IL-12 (PeproTech ...
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bioRxiv - Microbiology 2021Quote: Human IL-29/IL-28B (IFN-lambda 1/3) concentration in SARS-CoV-2 infected epithelium basal media was quantified using ELISA technics following manufacturer’s recommendations (R&D systems, Minneapolis, USA). 100 μl of media was used for each point.
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bioRxiv - Cancer Biology 2022Quote: ... culture medium was supplemented with 0.1 to 0.5 % BME (Cultrex Reduced Growth Factor Basement Membrane Extract, type 2, Pathclear, R&D Systems, 3533-005-02). We observed batch-to-batch variations of this product ...
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bioRxiv - Immunology 2022Quote: ... were incubated for 30 min at 20°C in 50 μl RPMI-HEPES in the absence of BCS with 2 μg/ml anti-ACE2 antibody (R&D Systems, #AF933) (Hoffmann et al. ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were blocked in 2% goat serum for 45 mins at room temperature and incubated with anti-Snail (R&D Systems, Minneapolis, MN), anti-Zeb1 (Invitrogen ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were kept in enriched N-2 medium with 10 ng/ml of basic fibroblast growth factor (R&D systems, Minneapolis, MN, USA) added every 24 h and medium changed every alternate day to keep the cells in an undifferentiated and proliferative state ...
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bioRxiv - Immunology 2021Quote: ... Cyclooxygenase-2-derived prostaglandin E2 (PGE2) and LTB4 release were measured with enzyme-linked immunosorbent assay kits (ELISA; R&D systems, Minneapolis, MN) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... 2) anti-CD31 antibodies (#550274, BD Biosciences, San Jose, CA, USA; #ab28364, Abcam, Cambridge, MA, USA; #BBA7, R&D systems, Minneapolis, MN, USA); 3 ...
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bioRxiv - Cell Biology 2021Quote: ... Media was replaced after 48 hours (Day 2) with RPMI-1640 supplemented with B27 without insulin and 5 µM IWP2 (R&D Systems #3533). Media was again replaced after an additional 48 hours (Day 4 ...
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bioRxiv - Neuroscience 2023Quote: ... 3mg of nuclear lysates were used for IP with 2 μg IgG or 5 μg primary antibodies (normal mouse IgG, Santa Cruz Biotechnology, sc-2025; normal rabbit IgG, R&D Systems, AB-105-C ...
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bioRxiv - Microbiology 2023Quote: ... Murine IP-10 production was measured from 100 μL of the supernatant from the BMDMs using Mouse CXCL10/IP-10/CRG-2 Duo Set ELISA (R&D systems, #Dy466) according to the manufacturer’s protocol.
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bioRxiv - Biophysics 2023Quote: ... and rTv20S-inhibitor complex were diluted with 50 mM HEPES pH 7.5 then mixed with 2 μM MV151 (R&D Systems #I-190). After MV151 addition ...
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bioRxiv - Neuroscience 2023Quote: ... ectodermal paired box 6 and SRY-box transcription factor 2 (PAX6/SOX2, Biolegend, San Diego, CA, USA / R&D Systems, Minneapolis, MN, USA), and forkhead box protein A2 (FOXA2 ...
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bioRxiv - Cell Biology 2022Quote: ... viability was measured with the BioTek Synergy HT plate reader using Gen5 software after a 2 hour incubation in Alamar blue (R&D Systems, AR002). Wells without cells and incubated in Alamar blue were used as a background reading.
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bioRxiv - Bioengineering 2023Quote: ... Plates were incubated with PBSTBA diluted 500 ng/mL (25 ng/well) recombinant biotinylated human ACE-2 (R&D Systems, Cat# BT933-020) for 2 h at RT ...
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bioRxiv - Immunology 2021Quote: ... 2×105 isolated TILs have been cultured in vitro in 96well plate in presence of 20ng/ml mouse IL-2 (R&D System 402-ML), 20ng/ml mouse IL-7 (R&D System 407-ML ...
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bioRxiv - Cell Biology 2021Quote: ... Thy1+ Rep-MEFs were cultured in ‘iMPC medium’ containing 2 μg/mL of dox and administrated three small molecules: 5 μM of Forskolin (F) (R&D Systems, 1099/50), 5 μM of RepSox (R ...
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bioRxiv - Neuroscience 2020Quote: ... mouse PFC fractions were dissociated with a tissue homogenizer in 100 μl of 0.5 × cell lysis buffer 2 diluted in PBS (R&D Systems., Minnesota, United State). Tissues were lysed at room temperature for 30 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... and from Days 2 to 5 CHIR99021 was withdrawn and replaced with 100 ng/mL Activin A (R&D Systems, 338-AC-050/CF). Media change was performed every day by discarding spent media ...
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bioRxiv - Immunology 2022Quote: ... Five million PBMC were suspended in 40 ml R10 supplemented with 10 ng/ml recombinant human IL-2 (R&D Systems, 202-IL-050) together with 10 million K562-mbIL21-41BBL feeder cells irradiated with 100 Gy ...
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bioRxiv - Bioengineering 2022Quote: ... Supernatant was collected and cytokines were quantified using mouse cytokine & chemokine convenience 6-Plex kit (IL-2, IL-4, IL-5, IL-10, IFN-γ and TNF-α; # LXSAMSM-06, R&D systems) using Luminex xMAP according to manufacturers protocols.
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bioRxiv - Developmental Biology 2022Quote: ... re-plated and incubated in 10-cm non-adherent Petri dishes in cSFDM containing 2 ng/ml rhActivin A (R&D Systems, #338-AC), 3 ng/ml rhBMP4 (R&D Systems ...
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bioRxiv - Cell Biology 2024Quote: ... Naïve T-cells were incubated in complete medium supplemented with 10 IU/mL recombinant human IL-2 (R&D Systems, 202-IL-050/CF). As a positive control ...
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bioRxiv - Cancer Biology 2023Quote: ... supernatants from the A20 and DO-11.10 cells were collected for IL-2 detection by enzyme-linked immunosorbent assays (ELISA) using commercially available sandwich-based kit (R&D systems, Cat# M2000), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... the quantity of IL-2 released by Jurkat-CD16 cells was measured in the cell supernatant by colorimetry using a human IL-2 DuoSet ELISA DY202-05 Kit (R&D Systems, Minneapolis, MN, USA).
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bioRxiv - Bioengineering 2021Quote: ... After 48 hours the cells were fed with stage 2 differentiation medium consisting of STEMDiff APEL supplemented with 50 ng/mL of VEGF 165 (R&D Systems, 293-VE-010), 25 ng/mL of BMP4 (R&D Systems ...
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bioRxiv - Bioengineering 2021Quote: ... PBMCs were stimulated for 2 days with CD3/CD28 T cell activation beads (Thermo # 11131D) in the presence of 40IU/mL IL2 (R&D Systems # 202-IL-010) and incubated at 37°C/5% CO2 ...
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bioRxiv - Bioengineering 2022Quote: ... PBMCs were stimulated for 2 days with CD3/CD28 T cell activation beads (Thermo # 11131D) in the presence of 40IU/mL IL2 (R&D Systems # 202-IL-010) in AIM V (Thermo ...
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bioRxiv - Bioengineering 2022Quote: ... PBMCs were stimulated for 2 days with CD3/CD28 T cell activation beads (Thermo # 11131D) in the presence of 40IU/mL IL2 (R&D Systems # 202-IL-010) in AIM V (Thermo ...
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bioRxiv - Cancer Biology 2020Quote: ... Endothelial cells were seeded at a density of 2×105 per well on the insert coated with Cultrex rat collagen I (R&D Systems, Minneapolis, Minnesota, USA), and the insert was placed in the above-mentioned six-well plates ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and incubated with recombinant SARS-CoV-2 Spike protein receptor binding domain (RBD) fused to the human IgG1 Fc domain (R&D Systems 10499-CV-100) or control recombinant human IgG1 Fc protein (R&D Systems 10499-CV-100 ...
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bioRxiv - Immunology 2020Quote: ... For iTreg cell differentiation we additionally added the following cytokines and blocking antibodies: rmIL-2 and rmTGF-β (both: R&D Systems, 5 ng/ml), anti-IL-4 (clone ...
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bioRxiv - Immunology 2022Quote: ... Fecal LCN2 levels in the supernatant fraction were estimated using a Duoset murine LCN-2 ELISA kit (R&D Systems DY1857-05, Minneapolis, MN, USA) based on provided directions.