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Citations for R&D Systems :
2951 - 3000 of 3214 citations for 8 1 Piperazinyl imidazo 1 2 a pyrazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... and Alexa Fluor 647-conjugated Human NCAM-1/CD56 (MC-480) live stain (catalog #FAB24081R-100UG) were purchased from R&D Systems (Minneapolis, MN, USA) as well as Human Three Germ Layer 3-Color Immunocytochemistry Kit (catalog #SC022 ...
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bioRxiv - Developmental Biology 2023Quote: The following primary antibodies were used at indicated concentrations for the immunostaining of compresstome sections or iDisco whole lobe samples: goat anti-EPCR (R&D Systems, AF2749, 1:100), rat anti-Endomucin (Santa Cruz Biotech ...
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bioRxiv - Neuroscience 2023Quote: The following antibodies were used for in vitro biotinylation (dilutions for each antibody are indicated in parentheses): chicken polyclonal antibody anti-neurofascin (1:1000; R&D Systems Cat# AF3235, RRID:AB_10890736), rabbit polyclonal anti-NrCAM (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: The following antibodies were used for immunofluorescence studies (dilutions for each antibody are indicated in parentheses): chicken polyclonal anti-neurofascin (1:500; R&D Systems Cat# AF3235, RRID:AB_10890736), chicken polyclonal anti-MAP2 (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... Media was replaced with DMEM containing gentamicin and ciprofloxacin HCl alone or containing antibiotics and 10% NCS and/or 200 ng/mL Recombinant Human Insulin growth factor 1 (R&D Systems Cat#: 291-G1) for indicated minpost-treatment ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were washed and blocked in PBS with 2% donkey serum and 2% bovine serum albumin for an hour and incubated with rabbit anti-IBA1 (WAKO, cat#019-19741, RRID:AB_839504, 1:500) and goat anti-GPNMB (R&D Systems, cat#AF2550, RRID:AB_416615, 3µg/ml) primary antibodies in PBS with 2% donkey serum overnight at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... A “type-1” like cytokine cocktail consisted of 10 ng/ml of recombinant mouse tumor necrosis factor-α (TNF-α, R&D Systems, Minneapolis, MN), 5 ng/ml of recombinant mouse interleukin-1β (IL-1β ...
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bioRxiv - Cancer Biology 2023Quote: Ascites collected from RD and IF mice (n=4/ group) were pooled and diluted 1:3 and applied in the adipokine array (ARY-013, R&D systems, Minneapolis, MN, USA). The proteome profiler adipokine array detects 38 adipokines (Table S4 ...
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bioRxiv - Cancer Biology 2023Quote: ... and the signalling ligands identified from the ligand-receptor analysis (Figure 4C): murine WNT5A (250 ng mL−1, R&D Systems 645-WN-010/CF), murine SEMA3A (250 ng mL−1 ...
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bioRxiv - Cell Biology 2022Quote: Proteasome activity was assessed similarly by incubating cells with genetic (7 days) or chemical (1 hour) perturbations with Me4BoVS (R&D system; I-190-050). After incubation at 37ºC for 2 hours ...
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bioRxiv - Developmental Biology 2023Quote: Flow cytometry performed within 1-6 days investigated NOTCH1 expression using anti-NECD1 antibody AF5267 and Notch ligand binding for DLL1-Fc (R&D Systems, Inc., Minneapolis, MN) and DLL4-Fc (Aro Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% FBS and 1% penicillin/streptomycin with macrophage-colony stimulating factor (M-CSF, 30 ng/mL; 416-ML, R&D systems, Minneapolis, MN, USA) for 4 days to obtain BMMs ...
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bioRxiv - Synthetic Biology 2023Quote: ... EV samples at a concentration of 1 × 1010 particles/mL were incubated with AF647-labelled anti-TSPAN2 antibody (R&D systems, FAB7876R-100UG, 0.5 µL) or a mixture of APC-labelled anti-CD9 (Miltenyi Biotech ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transferred to wet ice and the remaining surface receptor was labeled with 1:100 anti-rabbit antibody conjugated to PE (F0110, R&D Systems, Minneapolis, MN, USA) in FACS buffer for 30 min on ice and protected from light ...
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bioRxiv - Neuroscience 2023Quote: ... Medium was then changed to 500 µl OPC base medium (untreated control (=UC)) or OPC base medium supplemented with 1 ug/ml Osteopontin (cat#: 6359-OP, R&D Systems, Minneapolis, MN, USA) (OPN) ...
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bioRxiv - Neuroscience 2023Quote: ... A Nanoject II (Warner Instruments) was used to infuse 1 μl of vehicle (DMSO + saline) or AQ-RA 741 (R&D SYSTEMS, in DMSO + saline) before stimulus presentation on experimental days 1 and 2 under isoflurane anesthesia ...
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bioRxiv - Synthetic Biology 2023Quote: ... PD-1-DNR interaction with PD-L1 was assessed by staining with 1 μg Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein (R&D Systems, 1019-B7-100), followed by anti-hFc antibody.
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bioRxiv - Immunology 2024Quote: Caspase-1 levels in macaque serum samples were quantitatively determined using the Quantikine™ ELISA Human Caspase-1/ICE Immunoassay (Catalog# DCA100, R&D Systems, Minneapolis, MN). The assay was conducted following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... 1:100 diluted human plasmas and a dilution series of the indicated neutralizing anti-IFN antibodies (IFNβ, PBL assay science, 31410-1; IFNλ1: R&D systems, MAB15981; IFNλ2/3: R&D systems, MAB1587), or mock ...
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bioRxiv - Cancer Biology 2024Quote: ... spheres incubated under non adherent conditions for 5 days were challenged with the addition of 500 ng/ml human recombinant Netrin-1 (R&D Systems #6419-N1-025) for a period of 20 minutes before collection ...
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bioRxiv - Cancer Biology 2024Quote: Cell culture plates for cell invasion assays were prepared by adding 150 μL of 1 mg/mL Cultrex Reduced Growth Factor Basement Membrane Extract (R&D Systems, 3433-005-R1) to a 24-well cell culture insert with an 8 μm pore size (Falcon ...
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bioRxiv - Neuroscience 2024Quote: Animals were randomly assigned to experimental groups and injected intrathecally with saline or CCL2 (1 µg/rat, dissolved in 0.1% BSA in sterile saline; R&D Systems #3144-JE-025/CF) on postoperative days (PODs ...
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bioRxiv - Molecular Biology 2024Quote: ... non-fat dried milk in TTBS at room temperature for 1 h and incubated overnight at 4 °C with specific anti-IL-1β (R&D Systems, Minneapolis, MN, USA), anti-phospho-NF-κB p65 (Ser536 ...
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bioRxiv - Developmental Biology 2020Quote: ... or Tgfb1/2/3 ligands (10ng/ml, each) (R&D systems, 240-B/302-B2/243-B3) was placed in the upper and lower chambers ...
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bioRxiv - Immunology 2022Quote: ... CFSE-labelled cells were then resuspended in R10 media supplemented with recombinant IL-2 (R&D system) and 2-mercaptoethanol (Themo Fisher Scientific ...
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bioRxiv - Physiology 2019Quote: ... for 72 hours prior to stimulation with 10 ng/mL IFN-α (R&D Systems, 11101-2). Media was prepared by adding 120 μL of 10 mg/mL stock 13C11 15N2 L-tryptophan in 1x PBS to 240 mL of RPMI (Corning ...
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bioRxiv - Immunology 2021Quote: ... and 2.5 ng/ml recombinant mouse IL-15 and 100 units of IL-2 (R&D Systems).
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bioRxiv - Bioengineering 2021Quote: ... ELISA kits for FGF-2 and TGF-β1 were purchased from R&D Systems (Minneapolis, MN, USA) while the ELISA kit for Nodal was obtained from Abcam (Cambridge ...
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bioRxiv - Cell Biology 2020Quote: Supernatant levels of BMP2 were quantified using a BMP-2 Quantikine ELISA kit (R&D Systems #DBP200), without significant deviations from manufacturer’s instructions.
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bioRxiv - Physiology 2020Quote: ... Each treatment infusion was 2 μL of PBS or leptin (10μg/2μL, R&D Systems, Minneapolis, MN). Data collected 30 minutes prior and 30 minutes after the ICV injections were excluded from the analysis ...
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bioRxiv - Microbiology 2020Quote: ... The transduced cells were stained with anti-human ACE-2 polyclonal goat IgG (AF933, R&D Systems) primary antibody at 1 ug/mL and donkey anti-goat IgG conjugated to Alexa Fluor 488 (ab150129 ...
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bioRxiv - Immunology 2022Quote: ... The binding kinetics for the interaction between hmAbs and SARS-CoV-2 Spike protein (R&D Systems) was determined by injecting four concentrations of SARS-CoV-2 Spike (25 nM highest concentration ...
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bioRxiv - Cell Biology 2022Quote: ... mice were given intra-articular injections of recombinant mouse R-spondin 2 (500 ng, R&D Systems) or vehicle (phosphate-buffered saline ...
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bioRxiv - Cancer Biology 2021Quote: ... a standard direct ELISA was performed with DuoSet Ancillary Reagent Kit 2 (R&D systems, Minneapolis, MN). After coating wells with recombinant human EGFR (1μg/mL) ...
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bioRxiv - Cell Biology 2019Quote: ... the substrates were incubated for 2 h with human E-cadherin-Fc (R&D system, Minneapolis, MN) at 1 μg / cm2 ...
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bioRxiv - Developmental Biology 2021Quote: ... Media was changed every 2 days using differentiation media supplemented with 100ng/ml VEGF (R&D Systems) and 50ng/ml bFGF (R&D Systems) ...
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bioRxiv - Immunology 2020Quote: ... Collected ILCs were rested in 10ng/mL IL-2 and IL-7 (R&D Systems, Minneapolis, MN) in T-cell media (TCM ...
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bioRxiv - Microbiology 2022Quote: ... n = 4 each) were stained with mouse anti-SARS-CoV-2 N monoclonal antibody (R&D systems, Clone 1035111 ...
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bioRxiv - Genetics 2023Quote: ... and receptor activator of nuclear factor kappa-B ligand (RANKL) (2 ng/mL; R&D systems; USA) to induce osteoclastogenesis ...
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bioRxiv - Immunology 2023Quote: ... LCN2 in the clear supernatants was quantified using Mouse Lipocalin-2/NGAL DuoSet ELISA (R&D systems) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ELISA plates were coated with 2 µg/ml mouse anti-His tag antibody (R&D Systems, MAB050) and blocked as described above ...
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bioRxiv - Biochemistry 2023Quote: The human ADGF (Recombinant Human Adenosine deaminase 2/CECR1) #7518-AD-010 was from R&D Systems, Minneapolis ...
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bioRxiv - Immunology 2019Quote: ... Levels of IL-1β and TNF-α in 200 μl culture medium were measured by ELISA kits according to manufacturer’s instructions (DY401 for IL-1 β and DY410 for TNF-α, R&D systems, Minneapolis, MN, USA). Cleavage of pro-IL-1β and pro-caspase-1 was assessed by western blot of 50 μg of protein extracts of cell lysates ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were pre-treated for 1 day with vehicle control (0.1% BSA in 1X PBS) or 400 ng/ml human recombinant IL-1RA (R&D Systems, Minneapolis, MN; 280-RA/CF) 0 in the DMEM/10% FBE growth medium ...
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bioRxiv - Neuroscience 2020Quote: ... while MABs differentiation into myotubes was initiated in the opposite compartments using MAB differentiation medium containing 2% horse serum (Cat N° 16050122) and 1% sodium pyruvate in DMEM/F12 supplemented with 0.01 μg/ml recombinant human agrin protein (R&D Systems, Cat N° 6624-AG-050). At day 21 ...
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bioRxiv - Microbiology 2021Quote: ... PMA differentiated THP-1 cells were incubated 2 h prior to infection and maintained throughout with either,1 μg/mL mouse monoclonal anti-human IL-10 antibody (R&D Systems. Ref: AF-217-NA), or equivalent concentrations of human IgG (Invitrogen ...
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bioRxiv - Bioengineering 2019Quote: ... cells were plated at 1 million cells per well in 2mL mESC media (without LIF) + 25 μg/L Activin A (R&D Systems 338-AC-050/CF), 5 μg/L BMP4 (PeptroTech 315-27) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were starved in 0.1% FBS for 24 hours prior to addition of EGF (R&D systems #236-EG, diluted in 1 mg/ml BSA/PBS) to 25 ng/μl or carrier alone for 5 min at 37ºC ...
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bioRxiv - Molecular Biology 2023Quote: ... The sections were blocked with 5% skimmed milk in TBS-T at room temperature for 60 min and then incubated with rabbit anti-mMCP-6 antibody (R&D Systems, MN, USA, 1:500) at 4 °C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... HSC cells were cultured in serum-free DMEM with 1% PSG and subjected to the different treatments: TGFβ2 (2.5 ng/ml; R&D Systems, Minneapolis, MN, USA; for 3 d), the ROCK inhibitor Y27632 (10 μM ...